Difference between revisions of "Team:TU-Eindhoven/Project/Protocols"
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Digestion<br/> | Digestion<br/> | ||
Ligation<br/> | Ligation<br/> | ||
| − | LIU PCR</h6 | + | LIU PCR</h6> |
<h1>Gibson Assembly</h1> | <h1>Gibson Assembly</h1> | ||
Revision as of 09:05, 1 November 2017
General Protocols
General Necessities
Glycerol Stock
Streaking Glycerol Stock
Nanodrop
Agarose Gel Electrophoresis
SDS-Page
Traditional Cloning & BioBricking
PCR Amplification
PCR Purification
Digestion
Ligation
LIU PCR
Gibson Assembly
Vector Linearization
Gibson Assembly
Transformation, Protein Expression & Purification
Transformation into NovaBlue
Plating
Colony Picking and Colony PCR
Sequencing
Miniprep
Small culturing
Protein expression
Protein Purification using a His-tag
Protein Purification using a Strep-tag
Transformation, Protein Expression & Purification
Transformation into NovaBlue
Plating
Colony Picking and Colony PCR
Sequencing
Miniprep
Small culturing
Protein expression
Protein Purification using a His-tag
Protein Purification using a Strep-tag
