Difference between revisions of "Team:TU-Eindhoven/Project/Protocols"
| Line 25: | Line 25: | ||
Nanodrop<br/> | Nanodrop<br/> | ||
Agarose Gel Electrophoresis<br/> | Agarose Gel Electrophoresis<br/> | ||
| − | SDS-Page</h6 | + | SDS-Page</h6><br/> |
<h1>Traditional Cloning & BioBricking</h1> | <h1>Traditional Cloning & BioBricking</h1> | ||
| Line 32: | Line 32: | ||
Digestion<br/> | Digestion<br/> | ||
Ligation<br/> | Ligation<br/> | ||
| − | LIU PCR</h6> | + | LIU PCR</h6><br/> |
<h1>Gibson Assembly</h1> | <h1>Gibson Assembly</h1> | ||
<h6>Vector Linearization<br/> | <h6>Vector Linearization<br/> | ||
| − | Gibson Assembly< | + | Gibson Assembly</h6><br/> |
<h1>Transformation, Protein Expression & Purification</h1> | <h1>Transformation, Protein Expression & Purification</h1> | ||
| Line 47: | Line 47: | ||
Protein expression<br/> | Protein expression<br/> | ||
Protein Purification using a His-tag <br/> | Protein Purification using a His-tag <br/> | ||
| − | Protein Purification using a Strep-tag</h6><br/><br/> | + | Protein Purification using a Strep-tag</h6><br/> |
| + | |||
| + | <h1>Testing</h1> | ||
| + | <h6>Assay<br/> | ||
| + | Fluorescence measurements</h6><br/> | ||
Revision as of 09:08, 1 November 2017
General Protocols
General Necessities
Glycerol Stock
Streaking Glycerol Stock
Nanodrop
Agarose Gel Electrophoresis
SDS-Page
Traditional Cloning & BioBricking
PCR Amplification
PCR Purification
Digestion
Ligation
LIU PCR
Gibson Assembly
Vector Linearization
Gibson Assembly
Transformation, Protein Expression & Purification
Transformation into NovaBlue
Plating
Colony Picking and Colony PCR
Sequencing
Miniprep
Small culturing
Protein expression
Protein Purification using a His-tag
Protein Purification using a Strep-tag
Testing
Assay
Fluorescence measurements
