Difference between revisions of "Team:TU-Eindhoven/Project/Protocols"
| Line 30: | Line 30: | ||
</body> | </body> | ||
<h1>General Protocols</h1> | <h1>General Protocols</h1> | ||
| − | <h6>General Necessities | + | <h6>General Necessities<span style="display:inline-block; width:200px;"></span>Preparation of antibiotics, growth media, stock solutions and more.<br/> |
Glycerol Stock<br/> | Glycerol Stock<br/> | ||
Streaking Glycerol Stock<br/> | Streaking Glycerol Stock<br/> | ||
Revision as of 13:58, 1 November 2017
General Protocols
General NecessitiesPreparation of antibiotics, growth media, stock solutions and more.
Glycerol Stock
Streaking Glycerol Stock
Nanodrop
Agarose Gel Electrophoresis
SDS-Page
Traditional Cloning & BioBricking
PCR Amplification
PCR Purification
Digestion
Ligation
LIU PCR
Gibson Assembly
Vector Linearization
- Linearize the vector in which you want to insert the gBlocks
Gibson Assembly
Transformation, Protein Expression & Purification
Transformation into NovaBlue
Plating
Colony Picking and Colony PCR
Sequencing
Miniprep
Small culturing
Protein expression
Protein Purification using a His-tag
Protein Purification using a Strep-tag
Testing
Assay
Fluorescence measurements
