Difference between revisions of "Team:TU-Eindhoven/Project/Protocols"
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<h1>General Protocols</h1> | <h1>General Protocols</h1> | ||
| − | <h6>General Necessities<span style="display:inline-block; width: | + | <h6>General Necessities <span style="display:inline-block; width:100px;"></span>Preparation of antibiotics, growth media, stock solutions and more.<br/> |
| − | Glycerol Stock<br/> | + | Glycerol Stock <span style="display:inline-block; width:100px;"></span> Preparing the bacteria for long term storage in the -80 °C freezer.<br/> |
| − | Streaking Glycerol Stock<br/> | + | Streaking Glycerol Stock <span style="display:inline-block; width:100px;"></span> After storage in the -80 °C, the bacteria can be prepared for usage by streaking them on agar plates.<br/> |
| − | Nanodrop<br/> | + | Nanodrop<span style="display:inline-block; width:100px;"></span> Nanodrop is used to determine the concentration of a DNA sample.<br/> |
| − | Agarose Gel Electrophoresis<br/> | + | Agarose Gel Electrophoresis<span style="display:inline-block; width:100px;"></span>Agarose Gel Electrophoresis is used to determine the length of DNA samples.<br/> |
| − | SDS-Page</h6><br/> | + | SDS-Page<span style="display:inline-block; width:100px;"></span>SDS-Page is used to determine the size of a protein.</h6><br/> |
<h1>Traditional Cloning & BioBricking</h1> | <h1>Traditional Cloning & BioBricking</h1> | ||
Revision as of 14:04, 1 November 2017
General Protocols
General Necessities Preparation of antibiotics, growth media, stock solutions and more.
Glycerol Stock Preparing the bacteria for long term storage in the -80 °C freezer.
Streaking Glycerol Stock After storage in the -80 °C, the bacteria can be prepared for usage by streaking them on agar plates.
Nanodrop Nanodrop is used to determine the concentration of a DNA sample.
Agarose Gel ElectrophoresisAgarose Gel Electrophoresis is used to determine the length of DNA samples.
SDS-PageSDS-Page is used to determine the size of a protein.
Traditional Cloning & BioBricking
PCR Amplification
PCR Purification
Digestion
Ligation
LIU PCR
Gibson Assembly
Vector Linearization
- Linearize the vector in which you want to insert the gBlocks
Gibson Assembly
Transformation, Protein Expression & Purification
Transformation into NovaBlue
Plating
Colony Picking and Colony PCR
Sequencing
Miniprep
Small culturing
Protein expression
Protein Purification using a His-tag
Protein Purification using a Strep-tag
Testing
Assay
Fluorescence measurements
