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General Protocols

General Necessities
Preparation of antibiotics, growth media, stock solutions and more.
Glycerol Stock
Preparing the bacteria for long term storage in the -80 °C freezer.
Streaking Glycerol Stock
After storage in the -80 °C, the bacteria can be prepared for usage by streaking them on agar plates.
Nanodrop
Nanodrop is used to determine the concentration of a DNA sample.
Agarose Gel Electrophoresis
Agarose Gel Electrophoresis is used to determine the length of DNA samples.
SDS-Page
SDS-Page is used to determine the size of a protein.

Traditional Cloning & BioBricking

PCR Amplification
PCR Purification
Digestion
Ligation
LIU PCR

Gibson Assembly

Vector Linearization

Linearize the vector in which you want to insert the gBlocks
Gibson Assembly

Transformation, Protein Expression & Purification

Transformation into NovaBlue
Plating
Colony Picking and Colony PCR
Sequencing
Miniprep
Small culturing
Protein expression
Protein Purification using a His-tag
Protein Purification using a Strep-tag

Testing

Assay
Fluorescence measurements

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 </body>
 <h1>General Protocols</h1>
-<h6>General Necessities <span style="display:inline-block; width:100px;"></span>Preparation of antibiotics, growth media, stock solutions and more.<br/>
+<h6>General Necessities
-Glycerol Stock <span style="display:inline-block; width:100px;"></span> Preparing the bacteria for long term storage in the -80 °C freezer.<br/>
+<br/><i>Preparation of antibiotics, growth media, stock solutions and more.</i><br/>
-Streaking Glycerol Stock <span style="display:inline-block; width:100px;"></span> After storage in the -80 °C, the bacteria can be prepared for usage by streaking them on agar plates.<br/>
+Glycerol Stock
-Nanodrop<span style="display:inline-block; width:100px;"></span> Nanodrop is used to determine the concentration of a DNA sample.<br/>
+<br/><i>Preparing the bacteria for long term storage in the -80 °C freezer.</i><br/>
-Agarose Gel Electrophoresis<span style="display:inline-block; width:100px;"></span>Agarose Gel Electrophoresis is used to determine the length of DNA samples.<br/>
+Streaking Glycerol Stock
-SDS-Page<span style="display:inline-block; width:100px;"></span>SDS-Page is used to determine the size of a protein.</h6><br/>
+<br/><i>After storage in the -80 °C, the bacteria can be prepared for usage by streaking them on agar plates.</i><br/>
+Nanodrop
+<br/><i>Nanodrop is used to determine the concentration of a DNA sample.</i><br/>
+Agarose Gel Electrophoresis
+<br/><i>Agarose Gel Electrophoresis is used to determine the length of DNA samples.</i><br/>
+SDS-Page
+<br/><i>SDS-Page is used to determine the size of a protein.</i></h6><br/>
 <h1>Traditional Cloning & BioBricking</h1>

Difference between revisions of "Team:TU-Eindhoven/Project/Protocols"

Revision as of 14:13, 1 November 2017

General Protocols

Traditional Cloning & BioBricking

PCR Amplification PCR Purification Digestion Ligation LIU PCR

Gibson Assembly

Vector Linearization Linearize the vector in which you want to insert the gBlocks Gibson Assembly

Transformation, Protein Expression & Purification

Transformation into NovaBlue Plating Colony Picking and Colony PCR Sequencing Miniprep Small culturing Protein expression Protein Purification using a His-tag Protein Purification using a Strep-tag

Testing

Assay Fluorescence measurements

PCR Amplification
PCR Purification
Digestion
Ligation
LIU PCR

Vector Linearization

Linearize the vector in which you want to insert the gBlocks
Gibson Assembly

Transformation into NovaBlue
Plating
Colony Picking and Colony PCR
Sequencing
Miniprep
Small culturing
Protein expression
Protein Purification using a His-tag
Protein Purification using a Strep-tag

Assay
Fluorescence measurements