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</body> | </body> | ||
<h1>General Protocols</h1> | <h1>General Protocols</h1> | ||
− | <h6>General Necessities < | + | <h6>General Necessities |
− | Glycerol Stock < | + | <br/><i>Preparation of antibiotics, growth media, stock solutions and more.</i><br/> |
− | Streaking Glycerol Stock < | + | Glycerol Stock |
− | Nanodrop< | + | <br/><i>Preparing the bacteria for long term storage in the -80 °C freezer.</i><br/> |
− | Agarose Gel Electrophoresis< | + | Streaking Glycerol Stock |
− | SDS-Page< | + | <br/><i>After storage in the -80 °C, the bacteria can be prepared for usage by streaking them on agar plates.</i><br/> |
+ | Nanodrop | ||
+ | <br/><i>Nanodrop is used to determine the concentration of a DNA sample.</i><br/> | ||
+ | Agarose Gel Electrophoresis | ||
+ | <br/><i>Agarose Gel Electrophoresis is used to determine the length of DNA samples.</i><br/> | ||
+ | SDS-Page | ||
+ | <br/><i>SDS-Page is used to determine the size of a protein.</i></h6><br/> | ||
<h1>Traditional Cloning & BioBricking</h1> | <h1>Traditional Cloning & BioBricking</h1> |
Revision as of 14:13, 1 November 2017
General Protocols
General Necessities
Preparation of antibiotics, growth media, stock solutions and more.
Glycerol Stock
Preparing the bacteria for long term storage in the -80 °C freezer.
Streaking Glycerol Stock
After storage in the -80 °C, the bacteria can be prepared for usage by streaking them on agar plates.
Nanodrop
Nanodrop is used to determine the concentration of a DNA sample.
Agarose Gel Electrophoresis
Agarose Gel Electrophoresis is used to determine the length of DNA samples.
SDS-Page
SDS-Page is used to determine the size of a protein.
Traditional Cloning & BioBricking
PCR Amplification
PCR Purification
Digestion
Ligation
LIU PCR
Gibson Assembly
Vector Linearization
- Linearize the vector in which you want to insert the gBlocks
Gibson Assembly
Transformation, Protein Expression & Purification
Transformation into NovaBlue
Plating
Colony Picking and Colony PCR
Sequencing
Miniprep
Small culturing
Protein expression
Protein Purification using a His-tag
Protein Purification using a Strep-tag
Testing
Assay
Fluorescence measurements