Difference between revisions of "Team:TU-Eindhoven/Project/Protocols"
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SDS-Page</h6><br/> | SDS-Page</h6><br/> | ||
| − | <h1>Traditional Cloning & BioBricking</h1> | + | <h1>Traditional Cloning & BioBricking</h1> <ul> |
<h6>PCR Amplification<br/> | <h6>PCR Amplification<br/> | ||
PCR Purification<br/> | PCR Purification<br/> | ||
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<h1>Gibson Assembly</h1> | <h1>Gibson Assembly</h1> | ||
<h6>Vector Linearization<br/> | <h6>Vector Linearization<br/> | ||
| + | <li><span>Linearize the vector in which you want to insert the gBlocks<span></li> | ||
Gibson Assembly</h6><br/> | Gibson Assembly</h6><br/> | ||
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<h6>Assay<br/> | <h6>Assay<br/> | ||
Fluorescence measurements</h6><br/> | Fluorescence measurements</h6><br/> | ||
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Revision as of 13:56, 1 November 2017
General Protocols
General Necessities
Preparation of antibiotics, growth media, stock solutions and more.
Glycerol Stock
Streaking Glycerol Stock
Nanodrop
Agarose Gel Electrophoresis
SDS-Page
Traditional Cloning & BioBricking
- Linearize the vector in which you want to insert the gBlocks Gibson Assembly
PCR Amplification
PCR Purification
Digestion
Ligation
LIU PCR
Gibson Assembly
Vector Linearization
Transformation, Protein Expression & Purification
Transformation into NovaBlue
Plating
Colony Picking and Colony PCR
Sequencing
Miniprep
Small culturing
Protein expression
Protein Purification using a His-tag
Protein Purification using a Strep-tag
Testing
Assay
Fluorescence measurements
