Team:TU-Eindhoven/Project/Protocols

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General Protocols

General Necessities
Preparation of antibiotics, growth media, stock solutions and more.
Glycerol Stock
Streaking Glycerol Stock
Nanodrop
Agarose Gel Electrophoresis
SDS-Page

Traditional Cloning & BioBricking

    PCR Amplification
    PCR Purification
    Digestion
    Ligation
    LIU PCR

    Gibson Assembly

    Vector Linearization
  • Linearize the vector in which you want to insert the gBlocks
  • Gibson Assembly

    Transformation, Protein Expression & Purification

    Transformation into NovaBlue
    Plating
    Colony Picking and Colony PCR
    Sequencing
    Miniprep
    Small culturing
    Protein expression
    Protein Purification using a His-tag
    Protein Purification using a Strep-tag

    Testing

    Assay
    Fluorescence measurements

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