General Necessities Preparation of antibiotics, growth media, stock solutions and more.
Glycerol Stock Preparing the bacteria for long term storage in the -80 °C freezer.
Streaking Glycerol Stock After storage in the -80 °C, the bacteria can be prepared for usage by streaking them on agar plates.
Nanodrop Nanodrop is used to determine the concentration of a DNA sample.
Agarose Gel ElectrophoresisAgarose Gel Electrophoresis is used to determine the length of DNA samples.
SDS-PageSDS-Page is used to determine the size of a protein.
Traditional Cloning & BioBricking
PCR Amplification
PCR Purification
Digestion
Ligation
LIU PCR
Gibson Assembly
Vector Linearization
Linearize the vector in which you want to insert the gBlocks
Gibson Assembly
Transformation, Protein Expression & Purification
Transformation into NovaBlue
Plating
Colony Picking and Colony PCR
Sequencing
Miniprep
Small culturing
Protein expression
Protein Purification using a His-tag
Protein Purification using a Strep-tag
