Difference between revisions of "Team:TU-Eindhoven/Results"

 
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<h2>The TU Eindhoven team of 2017 has won a golden medal and was nominated for Best New Application!</h2>
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<h1>Overview of generated results</h1>
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<h6>During iGEM we generated many different sorts of results. In the beginning of iGEM we mostly worked on getting the right DNA plasmids, by using different methods, like Gibson assembly and Traditional Cloning. In this period, the most important results included agarose gels and sequencing result (they are presented in <a href="https://2017.igem.org/Team:TU-Eindhoven/Results/DNA">"DNA results"</a>). After this period, we had to test the expression of our designed proteins and instead of agarose gels, SDS-PAGE gels became more important. The last period is marked by the "real" experiments, where we could finally test if our designed system indeed worked as planned. To get an overview of all the steps we performed in the lab, go see our <a href="https://2017.igem.org/Team:TU-Eindhoven/Team/Notebook">"Notebook"</a> page. <br/><br/>
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<b>The results of our Proof of Concept can be found <a href="https://2017.igem.org/Team:TU-Eindhoven/Demonstrate">here.</a> </b></br></br>
  
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As iGEM is a broad competition based around synthetic biology, we also did many other things, for example thinking about safety and the future, which are part of <a href="https://2017.igem.org/Team:TU-Eindhoven/Human_Practices">"Human Practices"</a>. </br></br>
  
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<h1>Results</h1>
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Another important part was making a model of our system. It took a lot of time to start up, but in the end we got to simulate our system (<a href="https://2017.igem.org/Team:TU-Eindhoven/Results/Model">"Model Results"</a>). During our collaboration with Potsdam we discovered that they had no team member with experience with modeling, so we offered to also model their system, the results can be found below our own results, but also on their WIKI page: <a href="https://2017.igem.org/Team:Potsdam/Model">"Potsdam"</a>). Additionally, their lack of experience an the time it took for us to start up with the model, inspired us to design a software tool: <a href="https://2017.igem.org/Team:TU-Eindhoven/Software">"NFsim manual for dummies"</a>. </h6> </br></br>
  
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<p>Here you can describe the results of your project and your future plans. </p>
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<h1>Accomplishments in short</h1>
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<li><span>Successfully designed, expressed and characterized multivalent constructs that will gelate after an inducer is added.</span></li>
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<li><span>Modelled the behavior of GUPPI and made a general tool for simulating other constructs with the same principle.</span></li>
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<li><span>Contacted stakeholders and integrated their input for a safe and useful design of GUPPI.</span></li>
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<li><span>Uploaded sequences of parts, characterized expression in the pBAD vector and delivered a codon optimized mCherry part.</span></li>
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<h5>What should this page contain?</h5>
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See our <a href="https://2017.igem.org/Team:TU-Eindhoven/Results/Medal_criteria">"Medal Criteria"</a> page for a broad list of all our accomplishments during iGEM.
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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<h5>You should also describe what your results mean: </h5>
 
  
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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<h5> Project Achievements </h5>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<h5>Inspiration</h5>
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<p>See how other teams presented their results.</p>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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Latest revision as of 10:18, 15 December 2017

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The TU Eindhoven team of 2017 has won a golden medal and was nominated for Best New Application!

Overview of generated results

During iGEM we generated many different sorts of results. In the beginning of iGEM we mostly worked on getting the right DNA plasmids, by using different methods, like Gibson assembly and Traditional Cloning. In this period, the most important results included agarose gels and sequencing result (they are presented in "DNA results"). After this period, we had to test the expression of our designed proteins and instead of agarose gels, SDS-PAGE gels became more important. The last period is marked by the "real" experiments, where we could finally test if our designed system indeed worked as planned. To get an overview of all the steps we performed in the lab, go see our "Notebook" page.

The results of our Proof of Concept can be found here.

As iGEM is a broad competition based around synthetic biology, we also did many other things, for example thinking about safety and the future, which are part of "Human Practices".

Another important part was making a model of our system. It took a lot of time to start up, but in the end we got to simulate our system ("Model Results"). During our collaboration with Potsdam we discovered that they had no team member with experience with modeling, so we offered to also model their system, the results can be found below our own results, but also on their WIKI page: "Potsdam"). Additionally, their lack of experience an the time it took for us to start up with the model, inspired us to design a software tool: "NFsim manual for dummies".


Accomplishments in short

  • Successfully designed, expressed and characterized multivalent constructs that will gelate after an inducer is added.
  • Modelled the behavior of GUPPI and made a general tool for simulating other constructs with the same principle.
  • Contacted stakeholders and integrated their input for a safe and useful design of GUPPI.
  • Uploaded sequences of parts, characterized expression in the pBAD vector and delivered a codon optimized mCherry part.

See our "Medal Criteria" page for a broad list of all our accomplishments during iGEM.




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