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Gel electrophoresis after colony PCR showed that most colonies merely contained the original dimer (around 1700), but two colonies did give good results (around 4200). The sequencing services of StarSEQ confirmed that the formation of a tetrameric 14-3-3 with GFP was finally successful.</h6><br/><br/> | Gel electrophoresis after colony PCR showed that most colonies merely contained the original dimer (around 1700), but two colonies did give good results (around 4200). The sequencing services of StarSEQ confirmed that the formation of a tetrameric 14-3-3 with GFP was finally successful.</h6><br/><br/> | ||
− | <h6><div class="Figure_3"><img src="https://static.igem.org/mediawiki/2017/4/4c/T--TU-Eindhoven--14-3-3_tetramer_gel.png"> | + | <h6><div class="Figure_3"><img src="https://static.igem.org/mediawiki/2017/4/4c/T--TU-Eindhoven--14-3-3_tetramer_gel.png" width="500" height="500" /> |
<figcaption>Figure 1: 14-3-3 tetramer with GFP</figcaption></div></h6> | <figcaption>Figure 1: 14-3-3 tetramer with GFP</figcaption></div></h6> | ||
Revision as of 13:35, 1 November 2017