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<h6><div class="Figure_3"><img src="https://static.igem.org/mediawiki/2017/d/dc/T--TU-Eindhoven--methionine.png"> | <h6><div class="Figure_3"><img src="https://static.igem.org/mediawiki/2017/d/dc/T--TU-Eindhoven--methionine.png"> | ||
− | <figcaption>Figure | + | <figcaption>Figure 4: Primers insert extra 15 amino acids</figcaption></div></h6> |
<br><br> | <br><br> | ||
+ | <i>Second approach:</i><br> | ||
+ | We also planned on placing the construct in a pET28a(+) vector, with which we have more experience when it comes to expression. We designed two new gBlocks: one was quite similar to the one in pBAD-DEST49, which means it starts with Strep-tag II, followed by mCherry, ending with CT33. The other one starts with mCherry, followed by Strep-tag II, ending with CT33. These are called samples SM and MS respectively. These were placed into the pET28a(+) vector using NcoI and HindIII restriction sites and these also proved to be successful in gel electrophoresis of colony PCR and sequencing. The expected lengths are around 1200 bp, with sample SM being slightly shorter than MS. | ||
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+ | <h6><div class="Figure_3"><img src="https://static.igem.org/mediawiki/2017/a/ad/T--TU-Eindhoven--CT33_in_pET28.png" height="600" /> | ||
+ | <figcaption>Figure 5: Two different CT33 constructs (MS and SM) cloned into pET28a(+) vector</figcaption></div></h6> | ||
+ | <br><br> | ||
</html> | </html> | ||
{{TU-Eindhoven_footer}} | {{TU-Eindhoven_footer}} |
Revision as of 13:48, 1 November 2017