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| − | .go .four:hover{background-position:-95px -190px}
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| − | .go .bottom{background-position:-8px -240px;height:60px}
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| − | .go .bottom:hover{background-position:-95px -240px}
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| − | </style>
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| − | <style>
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| − | body {
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| − | background-color:#e8f1f5;
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| − | font-size: 16px;
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| − | z-index:9999;
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| − | }
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| − | #title hr {
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| − | border: 0 none;
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| − | height: 12px;
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| − | margin: 1.3em auto;
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| − | width: 63px;
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| − | #title h1{
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| − | clear: both;
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| − | <div class="container"></div>
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| − | <div id="title">
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| − | <h1>Attributions</h1>
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| − | <hr>
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| − | </div>
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| − | <div id="tongyong">
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| − | <section class="main">
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| − | <div id="gotop" name="gotop"></div>
| + | |
| − | <h2>What We Did in This Summer</h2>
| + | |
| − | <p>In this summer, we designed genetic circuits, validated new technology and achieved application of heavy metal enrichment, for the first time on a cell chasis with synthetic genome. </p>
| + | |
| − | <p>We started from synthetic yeast to acquire more innovative genetic circuit.</p>
| + | |
| − | <p>We utilized the forefront of genome rearrangement technique, SCRaMbLE, to obtain double resistance to copper and cadmium by rapid evolution.</p>
| + | |
| − | <p>We upgraded exsisting parts for the purpose of more sensitive biosensor which had a wider response range to copper.</p>
| + | |
| − | <p>We employed our genetic circuit to realize biological enrichment of copper and cadmium.</p>
| + | |
| − | <p>We successfully modelled bistability, biosensor and biological adsorption.</p>
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | <h3>Team members</h3>
| + | |
| − |
| + | |
| − |
| + | |
| − | <p><i>Hui Xu</i>, the instructor of our team, He tirelessly and patiently assisted us in project design, experiment operation, wiki construction, human practice，and modeling.</p>
| + | |
| − | | + | |
| − | <p><i>Mingzhe Han</i>, the student leader of Team Tianjin this year, is in charge of the overall managment, Cu ion's detection, error-prone PCR, and part of verification of Mating Switcher.</p>
| + | |
| − | <p><i>Xinyu Chen</i>, is responsible for establishment of mating switcher characterization and also participate in biosensor for Cu Ion's detection experiment.</p>
| + | |
| − | <p><i>Xinyue Cao</i>, a primary experimental operator for SCRaMbLE and a designer of flocculation system, is also responsible for the knockout of <i>HMRa</i>. Besides, she is one of the speakers for <i>CCiC</i> meeting and giant jamboree presentation.</p>
| + | |
| − | <p><i>Li Sun</i>, one of the members of experiment, is responsible for lab safety. Besides, she also offered help for Human Practice.</p>
| + | |
| − | <p><i>Xinru Wang</i>, one of principals for human practice, is also responsible for collecting some data for drawing the pollution map.</p>
| + | |
| − | <p><i>Weixian Fu</i>, modeling constructor of Bistable Model, Functional switch Model and Global Cu and Cd Pollution Map，is also partly responsible for experiment operation.</p>
| + | |
| − | <p><i>Lingyi Zhou</i>, a successful lab operator, spared time to edit the handbook, drew illustrations and make the cartoon for our wiki.</p>
| + | |
| − | <p><i>Manlin Yang</i>, the main art; the designer of team clothes, logo, poster and presentation.</p>
| + | |
| − | <p><i>Yushuang Wang</i>, one of the primary experiment operators for SCRaMbLE and a designer of flocculation system, is responsible for part of Wiki writing. Also, she is one of the speakers.</p>
| + | |
| − | <p><i>Yangteng Ou</i>, one of the experiment operators for SCRaMbLE and a designer of flocculation parts, is also involved in writing wiki, producing ideas, making videos, and doing the last presentation.</p>
| + | |
| − | <p><i>Shiyu Zhang</i>, main principal for experiment operation, is responsible for the verification of mating swicher system.</p>
| + | |
| − | <p><i>Wenqing Guo</i>, main principal for designing and constructing the devise of mating type switcher, is also the team photograoher.</p>
| + | |
| − | <p><i>Lin Tu</i>, one of the operators for experiment, participated in constructing the HO vector.</p>
| + | |
| − | <p><i>Yan Zheng</i>, a experimental operator of both Separation of different irons and HO vector, is also involved in writing Wiki.</p>
| + | |
| − | <p><i>Zhou Guo</i>, main principal of experiment in Separation of different irons . </p>
| + | |
| − | <p><i>Yangyang Miao</i>, a primary experimental operator and one of designers of Mating Switcher construction, is a multifunctional assistant who likes helping other partners with their experiment. Besides, he is also responsible for modeling in biosensor.</p>
| + | |
| − | <p><i>Yixuan Chen</i>, main principal of human practice and partly constructed the project. </p>
| + | |
| − | <p><i>Xiaopo Cheng</i>, one of the primary experimental operators for enrichment of heavy metals, is also responsible for modeling.</p>
| + | |
| − | <p><i>Gefei Zhang</i>, the wiki designer, is responsible for the design and construction of our wiki.</p>
| + | |
| − | <p><i>Zichen Liu</i>, the designer of our wiki, is responsible for the construction of our wiki.</p>
| + | |
| − | | + | |
| − | | + | |
| − | <h3>General Support</h3>
| + | |
| − | <p><a href="http://www.tju.edu.cn/english/">Tianjin University</a>, for their support in the competition.</p>
| + | |
| − | <p><a href="http://chemeng.tju.edu.cn/en/;jsessionid=464E46A052D5A36E75B4FD6FAED3F76A">School of chemical engineering and technology, Tianjin University</a>, for their support in the competition.</p>
| + | |
| − | <p>Our instructor <i><a href="http://chemeng.tju.edu.cn/cn/szdw?type=detail&id=262">Prof. Yingjin Yuan</a></i> and <i><a href="http://chemeng.tju.edu.cn/cn/szdw?type=detail&id=259">Dr. Bingzhi Li</a></i> : tremendous thanks for their constructive suggestions and meticulous guidance to our project.</p>
| + | |
| − | | + | |
| − | | + | |
| − | <h3>Verification of Mating Swicher Support</h3>
| + | |
| − | <p><i>Dr. Yi Wu</i>, The builder of synthetic chromosome <i>synX</i> who generously gave us the yeast <i>synX</i> and many useful suggestions to handle all kinds of experiment problems, supporting the iGEM team Tianjin all the time.</p>
| + | |
| − | <p><i>Dr. Ruiying Zhu</i>, generously sponsored us with plasmid pRS416 and the E.coli that carries the yEmRFP gene. Also she commented on our thoughts, which helped us establishing the system over and over again.</p>
| + | |
| − | <p><i>Dr. Xiaole Wu</i>, generously sponsored us with yEmRFP plasmid.</p>
| + | |
| − | <p><i>Dr. Sijie Zhou</i>, instructed us in some key procedures.</p>
| + | |
| − | <p><i>Prof. Kun Zhao</i>, gave us permission of using the fluorescence microscope.</p>
| + | |
| − | <p><i>Prof. Weiwen Zhang</i>, who gave us permission of using the fluorescence spectrophotometer.<p>
| + | |
| − | <p><i>Dr. Tao Sun</i> and <i>Dr. Huaqing Liu</i>, taught us the use of fluorescence spectrophotometer and the fluorescence microscope, as well as kindly gave us suggestions about design and measurement.</p>
| + | |
| − | | + | |
| − | | + | |
| − | <h3>Resistance to Heavy Metals Support</h3>
| + | |
| − | <p><i>Yalu Ma</i>, the teacher of inorganic chemistry at the school of science Tianjin University, borrowed us 0.3g of the cadmium ion.</p>
| + | |
| − | <p><i>Dr. Sijie Zhou</i>, gave us a lot of help on constructing CRE vector and SCRaMbLE’s method.</p>
| + | |
| − | | + | |
| − | | + | |
| − | <h3>Separation of Different Irons Support</h3>
| + | |
| − | <p><i>Jing Chang</i>, a kind teacher who gave us permission of using the Atomic Absorption Spectroscopy for measuring the concentration of copper ion and cadmium. </p>
| + | |
| − | | + | |
| − | | + | |
| − | <h3>Human Practice Support</h3>
| + | |
| − | <p><a href="https://www.genscript.com/">Genscript</a> sponsors us 40000 CNY</p>
| + | |
| − | <p><a href="https://www.enzyme.com/">Enzyme</a> sponsors us 5000CNY and some biology reagent which costs 10000 CNY</p>
| + | |
| − | <p><a href="http://www.generalbiol.com/">General Biology</a> sponsors us 15000 CNY</p>
| + | |
| − | <p><a href="http://www.ameritechsc.com.cn/">Ameritech</a> sponsors us 6000 CNY</p>
| + | |
| − | <p><a href="https://www.genewiz.com.cn/">Genewiz</a> sponsors us 10000 CNY</p>
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| − | </section>
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| − | </div>
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| − | <div class="go">
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| − | <a title="top" class="top" href="#gotop"></a>
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| − | <a title="middle" class="feedback" href="#middle"></a>
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| − | <a title="22" class="two" href="#two"></a>
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| − | <a title="33" class="three" href="#three"></a>
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| − | <a title="44" class="four" href="#four"></a>
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| − | <a title="bottom" class="bottom" href="#gobottom"></a>
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| − | function(callback) {
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| − | {{:Team:Tianjin/Templates/footer}}
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