Difference between revisions of "Team:Toronto/Attributions"

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<div class="subsection">
 
<div class="subsection">
 
<h2>Wet Lab</h2>
 
<h2>Wet Lab</h2>
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<h3>Biozone</h3>
 
<h3>Biozone</h3>
 
<ul>
 
<ul>
 
<li>Dr. Sean Caffrey - Providing Lab Space</li>
 
<li>Dr. Sean Caffrey - Providing Lab Space</li>
 
<li>Dr. Grant Allen - Chemical Engineering - Providing Lab Space</li>
 
<li>Dr. Grant Allen - Chemical Engineering - Providing Lab Space</li>
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<li>
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<li>Dr. Elizabeth Edwards - Providing Lab space</li>
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Dr. Elizabeth Edwards <br>
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−
- Providing Lab space
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−
</li>
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<li>Dr. Radhakrishnan Mahadevan - supervisor</li>
 
<li>Dr. Radhakrishnan Mahadevan - supervisor</li>
 
<li>Endang Susilawati - BioZone Lab manager - Equipment & Biosafety training, Emotional Support</li>
 
<li>Endang Susilawati - BioZone Lab manager - Equipment & Biosafety training, Emotional Support</li>
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<li>Dr. Grant Allen - Chemical Engineering - Providing Lab Space</li>
 
<li>Dr. Grant Allen - Chemical Engineering - Providing Lab Space</li>
 
</ul>
 
</ul>
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<p>All the biological materials were handled in open bench areas. Escherichia coli (E.coli) was the chassis organism used in our study, which is exempt from the requirements of the NIH Guidelines<sup><a href="#ref3">[3]</a></sup>, as a risk group 1 organism<sup><a href="#ref4">[4]</a></sup> . We fused the protein binding domain of LacI (from E.coli) and the light oxygen voltage domain (LOV) of Avena sativa.</p>
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<p>We used a <span class="text-red">CI repressor</span> protein from Escherichia phage lambda in our switch. This sequence was obtained from a peer-reviewed article and has no indication of hazardous impact on eukaryotic cell lines.</p>
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<h3>Mahadevan Lab</h3>
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<p>The anti-CRISPR protein, <span class="text-red">AcrIIA4</span>, was obtained from Listeria monocytogenes prophages (risk group 1). The protein has been expressed in both bacterial and human cell lines in literature with no measurable toxicity.</p>
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<ul>
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<p>The anti-microbial resistance factors in our project were <span class="text-red">Standard Kanamycin</span> and <span class="text-red">Chloramphenicol</span> resistance casettes, which are commonly used in research and pose little health risk.</p>
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<li>Christian Euler - provided the sequence for LacILOV, pKDL071 plasmid, design help, troubleshooting assistance, Troubleshooting help and equipment training</li>
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<p>Our engineered system does not have any risk to humans or the environment since our <span class="text-red">Cas9</span> protein only targets metabolic genes like <span class="text-red">araC</span> in E.Coli as well as reporter genes like <span class="text-red">GFP</span> or <span class="text-red">mCherry</span>. Standard decontamination and containment through thermal sterilization and certified waste disposal would also mitigate any potential release in the environment.</p>
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<li>Ruhi Choudhary</li>
 +
<li>Kevin Correira - Gave us our nickname (wolf pack)</li>
 +
<li>Patrick Diep</li>
 +
<li>Kayla Nemr</li>
 +
<li>Naveen Venayak</li>
 +
<li>Aditya Vik Pandit</li>
 +
<li>Scott Proulx</li>
 +
<li>Kaushik Raj Venkatesan</li>
 +
<li>Benjamin Stordy</li>
 +
</ul>
 +
 
 +
<h3>Troubleshooting</h3>
 +
<ul>
 +
<li>Anna ????? - plate reader equipment training</li>
 +
<li>Tatiana ????? - Liquid Nitrogen Training</li>
 +
<li>Phil ????? - Package delivery</li>
 +
<li>Korchesca ????? - Yakunin Lab</li>
 +
</ul>
 +
 
 +
<h3>Maxwell Lab</h3>
 +
<ul>
 +
<li>Dr. Maxwell - antiCRISPR help</li>
 +
<li>Bianca - help in selecting specific Acr, project guidance regarding anticrispr portion of the project</li>
 +
</ul>
 +
 
 +
<h3>Others</h3>
 +
<ul>
 +
<li>Victoria’s lab - CRISPR assays ?????</li>
 +
<li>Guy from McMillan’s Lab - Project feedback ?????</li>
 +
</ul>
 
</div>
 
</div>
 
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<!-- subsection 2 END -->
  
 
<!-- subsection 3 -->
 
<!-- subsection 3 -->
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<div class="subsection">
 
<div class="subsection">
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<h2>Human Practices</h2>
 
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<p>In terms of future applications, temporal control of Cas9 activity through our switch could reduce off-target edits and increase the fidelity of genomic integrations through homology-directed repair, which should improve the safety of this technology. Although, from an ethical standpoint, there are several key shareholders including patients, medical practitioners and legislative and executive agencies that could be impacted by human gene editing. Thus, we conducted a systematic analysis of the considerations of each of those groups through interviews and a collaborative podcast series.</p>
 
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</div>
 
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<!-- subsection 3 END -->
 
  
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<hr>
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<h3>Vic Records</h3>
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<!-- Reference Subsection -->
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<ul>
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<div id="content-yellow" class="subsection">
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<li>Aaron (don’t know his last name ask Devaki) ?????</li>
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<h2 class="text-cyan">References</h2>
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</ul>
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<ol>
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−
<li id="ref1">Canadian Biosafety Standards and Guidelines - <a href="https://www.canada.ca/en/public-health/services/canadian-biosafety-standards-guidelines.html">https://www.canada.ca/en/public-health/services/canadian-biosafety-standards-guidelines.html</a></li>
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<h3>Video Interviews</h3>
−
<li id="ref2">Biosafety Team in the Office of Environmental Health and Safety - <a href="https://ehs.utoronto.ca/our-services/biosafety/">https://ehs.utoronto.ca/our-services/biosafety/</a></li>
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<ul>
−
<li id="ref3">NIH Guidelines - <a href="https://www.canada.ca/en/public-health/services/laboratory-biosafety-biosecurity/pathogen-safety-data-sheets-risk-assessment.html">https://www.canada.ca/en/public-health/services/laboratory-biosafety-biosecurity/pathogen-safety-data-sheets-risk-assessment.html</a></li>
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<li>Alice Wong</li>
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<li id="ref4">iGEM Risk Groups - <a href="https://2017.igem.org/Safety/Risk_Groups">https://2017.igem.org/Safety/Risk_Groups</a></li>
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<li>Anita Cameron</li>
−
</ol>
+
<li>Dr. Christopher Brittain</li>
 +
<li>Rabbi Michael Stroh</li>
 +
<li>Rabbi Aaron Greenberg</li>
 +
<li>Dr. Laura Prochazka</li>
 +
<li>Dr. Margaret Cheng</li>
 +
<li>Dr. Rodrigo Fernandez-Gonzalez</li>
 +
<li>Dr. Ronald Cohn</li>
 +
</ul>
 +
 
 +
<h3>Podcast</h3>
 +
<ul>
 +
<li>Jordan Thompson</li>
 +
<li>Dr. David McMillen</li>
 +
<li>iGEM Estonia</li>
 +
<li>iGEM Paris</li>
 +
<li>iGEM Uppsala</li>
 +
<li>iGEM Copenhagen</li>
 +
<li>Dr. Petri Lahtvee</li>
 +
</ul>
 +
 
 
</div>
 
</div>
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<!-- Reference Subsection END -->
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<!-- subsection 3 END -->
  
 
</div>
 
</div>

Revision as of 05:32, 31 October 2017

Attributions

Over the course of this project, iGEM Toronto has had considerable amount of help to overcome many hurdles. We will be forever in debt to the following individuals and organizations without whom this project would have been difficult to make a success.

Wet Lab

Biozone

  • Dr. Sean Caffrey - Providing Lab Space
  • Dr. Grant Allen - Chemical Engineering - Providing Lab Space
  • Dr. Elizabeth Edwards - Providing Lab space
  • Dr. Radhakrishnan Mahadevan - supervisor
  • Endang Susilawati - BioZone Lab manager - Equipment & Biosafety training, Emotional Support
  • Dr. Grant Allen - Chemical Engineering - Providing Lab Space
  • Dr. Grant Allen - Chemical Engineering - Providing Lab Space

Mahadevan Lab

  • Christian Euler - provided the sequence for LacILOV, pKDL071 plasmid, design help, troubleshooting assistance, Troubleshooting help and equipment training
  • Ruhi Choudhary
  • Kevin Correira - Gave us our nickname (wolf pack)
  • Patrick Diep
  • Kayla Nemr
  • Naveen Venayak
  • Aditya Vik Pandit
  • Scott Proulx
  • Kaushik Raj Venkatesan
  • Benjamin Stordy

Troubleshooting

  • Anna ????? - plate reader equipment training
  • Tatiana ????? - Liquid Nitrogen Training
  • Phil ????? - Package delivery
  • Korchesca ????? - Yakunin Lab

Maxwell Lab

  • Dr. Maxwell - antiCRISPR help
  • Bianca - help in selecting specific Acr, project guidance regarding anticrispr portion of the project

Others

  • Victoria’s lab - CRISPR assays ?????
  • Guy from McMillan’s Lab - Project feedback ?????

Vic Records

  • Aaron (don’t know his last name ask Devaki) ?????

Video Interviews

  • Alice Wong
  • Anita Cameron
  • Dr. Christopher Brittain
  • Rabbi Michael Stroh
  • Rabbi Aaron Greenberg
  • Dr. Laura Prochazka
  • Dr. Margaret Cheng
  • Dr. Rodrigo Fernandez-Gonzalez
  • Dr. Ronald Cohn

Podcast

  • Jordan Thompson
  • Dr. David McMillen
  • iGEM Estonia
  • iGEM Paris
  • iGEM Uppsala
  • iGEM Copenhagen
  • Dr. Petri Lahtvee

General Pages

Wet Lab

Dry Lab

Human Practices

iGEM Toronto