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− | {{UAlberta | + | {{UAlberta}} |
− | + | ||
<html> | <html> | ||
<head> | <head> | ||
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<meta name="viewport" content="width=device-width, initial-scale=1"> | <meta name="viewport" content="width=device-width, initial-scale=1"> | ||
</head> | </head> | ||
− | <body> | + | <body> |
− | <div id="ua- | + | |
+ | <nav id="ua-navbar" class="navbar navbar-default navbar-fixed-top"> | ||
+ | <div class="container"> | ||
+ | <div class="navbar-header"> | ||
+ | <button type="button" class="navbar-toggle collapsed" data-toggle="collapse" data-target="#navbar-collapse" aria-expanded="false"> | ||
+ | <span class="sr-only">Toggle navigation</span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | </button> | ||
+ | <a class="navbar-brand" href="#"><img src="https://static.igem.org/mediawiki/2017/6/65/T--UAlberta--Logo-Header.svg" height="50px" width="auto" /></a> | ||
+ | </div> | ||
+ | <div id="navbar-collapse" class="collapse navbar-collapse"> | ||
+ | <ul class="nav navbar-nav navbar-right"> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Team <span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="#">Team</a></li> | ||
+ | <li><a href="#">Collaborations</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Project <span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="#">Description</a></li> | ||
+ | <li><a href="#">Design</a></li> | ||
+ | <li><a href="#">Experiments</a></li> | ||
+ | <li><a href="#">Notebook</a></li> | ||
+ | <li><a href="#">Interlab</a></li> | ||
+ | <li><a href="#">Contribution</a></li> | ||
+ | <li><a href="#">Model</a></li> | ||
+ | <li><a href="#">Results</a></li> | ||
+ | <li><a href="#">Demonstrate</a></li> | ||
+ | <li><a href="#">Improve</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Parts <span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="#">Parts</a></li> | ||
+ | <li><a href="#">Basic Parts</a></li> | ||
+ | <li><a href="#">Composite Parts</a></li> | ||
+ | <li><a href="#">Part Collection</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Human Practices <span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="#">Silver HP</a></li> | ||
+ | <li><a href="#">Integrated and Gold</a></li> | ||
+ | <li><a href="#">Public Engagement</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"> | ||
+ | <a href="#" class="dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Awards <span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="#">Applied Design</a></li> | ||
+ | <li><a href="#">Entrepreneurship</a></li> | ||
+ | <li><a href="#">Hardware</a></li> | ||
+ | <li><a href="#">Measurement</a></li> | ||
+ | <li><a href="#">Model</a></li> | ||
+ | <li><a href="#">Plant</a></li> | ||
+ | <li><a href="#">Software</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | </nav> | ||
+ | |||
+ | <div id="ua-background" class="carousel slide carousel-fade" data-ride="carousel"> | ||
+ | <div class="carousel-inner" role="listbox"> | ||
+ | <div class="item active"> | ||
+ | </div> | ||
+ | <div class="item"> | ||
+ | </div> | ||
+ | <div class="item"> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="ua-home" class="text-center"> | ||
<div class="overlay"> | <div class="overlay"> | ||
<div class="container-fluid"> | <div class="container-fluid"> | ||
<div class="row"> | <div class="row"> | ||
− | <div class="col-lg- | + | <div class="col-lg-2 col-md-2 hidden-sm hidden-xs"> |
+ | <a href="#ua-background" class="left carousel-control" data-slide="prev"> | ||
+ | <span class="glyphicon glyphicon-chevron-left"></span> | ||
+ | <span class="sr-only">Previous</span> | ||
+ | </a> | ||
+ | </div> | ||
+ | <div class="col-lg-8 col-md-8 col-sm-12 col-xs-12"> | ||
<div id="ua-header"> | <div id="ua-header"> | ||
− | <h1> | + | <h1>Welcome to team: <strong>UAlberta</strong></h1> |
+ | <p>University of Alberta, Edmonton, Canada</p> | ||
</div> | </div> | ||
− | <a href="#ua- | + | <a href="#ua-home-abstract" class="fa fa-angle-down smooth"></a> |
+ | <ol class="carousel-indicators"> | ||
+ | <li data-slide-to="0" class="active" data-target="#ua-background"></li> | ||
+ | <li data-slide-to="1" data-target="#ua-background"></li> | ||
+ | <li data-slide-to="2" data-target="#ua-background"></li> | ||
+ | </ol> | ||
+ | </div> | ||
+ | <div class="col-lg-2 col-md-2 hidden-sm hidden-xs"> | ||
+ | <a href="#ua-background" class="right carousel-control" data-slide="next"> | ||
+ | <span class="glyphicon glyphicon-chevron-right"></span> | ||
+ | <span class="sr-only">Next</span> | ||
+ | </a> | ||
</div> | </div> | ||
</div> | </div> | ||
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</div> | </div> | ||
− | <div id="ua- | + | <div id="ua-home-abstract" class="text-center"> |
<div class="container"> | <div class="container"> | ||
+ | <h2>Abstract</h2> | ||
+ | <p>Team UAlberta aims to develop a buoyancy-based screening system for protein-protein interactions in <i>Escherichia coli</i>.</p> | ||
<div class="row"> | <div class="row"> | ||
− | <div class="col-lg- | + | <div class="col-lg-4 col-md-4 col-sm-4 col-xs-12"> |
− | < | + | <div class="col-lg-2 col-md-2 col-sm-2 col-xs-12"> |
− | + | <span class="fa fa-asterisk fa-3x" aria-hidden="true"></span> | |
− | <p> | + | </div> |
− | < | + | <div class="col-lg-10 col-md-10 col-sm-10 col-xs-12"> |
− | + | <p>What are the problems being addressed?</p> | |
− | + | </div> | |
− | + | </div> | |
− | <p> | + | <div class="col-lg-4 col-md-4 col-sm-4 col-xs-12"> |
− | < | + | <div class="col-lg-2 col-md-2 col-sm-2 col-xs-12"> |
− | < | + | <span class="fa fa-asterisk fa-3x" aria-hidden="true"></span> |
− | + | </div> | |
− | <p> | + | <div class="col-lg-10 col-md-10 col-sm-10 col-xs-12"> |
− | + | <p>What goals are we tying to achieve?</p> | |
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-lg-4 col-md-4 col-sm-4 col-xs-12"> | ||
+ | <div class="col-lg-2 col-md-2 col-sm-2 col-xs-12"> | ||
+ | <span class="fa fa-asterisk fa-3x" aria-hidden="true"></span> | ||
+ | </div> | ||
+ | <div class="col-lg-10 col-md-10 col-sm-10 col-xs-12"> | ||
+ | <p>How will we accomplish those goals?</p> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
+ | <a class="smooth" href="#ua-home-info">Read more</a> | ||
</div> | </div> | ||
</div> | </div> | ||
+ | |||
+ | <div id="ua-home-project" class="text-center"> | ||
+ | <div class="overlay"> | ||
+ | <div class="container"> | ||
+ | <h2>Project</h2> | ||
+ | <div id="ua-carousel-project" class="owl-carousel owl-theme"> | ||
+ | <div class="item"><a class="smooth" href="#ua-home-info"><span class="fa fa-cogs fa-lg" aria-hidden="true"></span>Goals</a></div> | ||
+ | <div class="item"><a class="smooth" href="#ua-home-info"><span class="fa fa-flask fa-lg" aria-hidden="true"></span>Project</a></div> | ||
+ | <div class="item"><a class="smooth" href="#ua-home-info"><span class="fa fa-check-circle-o fa-lg" aria-hidden="true"></span>Progress</a></div> | ||
+ | <div class="item"><a class="smooth" href="#ua-home-info">Project</a></div> | ||
+ | <div class="item"><a class="smooth" href="#ua-home-info">Project</a></div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="ua-home-info" class="text-center"> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="information-tabs"> | ||
+ | <div id="project-tab" class="info-tab active"> | ||
+ | <div class="col-lg-12 col-md-12"> | ||
+ | <h2>Project Description</h2> | ||
+ | <p>Team UAlberta aims to develop a buoyancy-based screening system for protein-protein interactions in <i>Escherichia coli</i>. This system relies on a bacterial two-hybrid system (BACTH) where the reconstitution of the T18 and T25 domains of adenylate cyclase, due to the interaction between two proteins fused to the two domains, will restore the activity of the adenylate cyclase depending on the strength of the protein-protein interaction. Reconstitution of adenylate cyclase restores the production of cAMP, which will then upregulate a cluster of genes controlling gas vesicle production, therefore conferring buoyancy to <i>E. coli </i>cells. Thus, the observed buoyancy in bacteria is ultimately dependent on the strength of the protein-protein interaction. The purpose of this system is to utilize buoyancy to physically separate bacteria expressing successful protein interactions from unsuccessful variants. Team UAlberta will first test and characterize the system using pairs of proteins with well-characterized interactions, such as leucine zippers. After initial testing, Team UAlberta aims to use the system in conjunction with directed evolution to engineer a potent peptide inhibitor of NHERF2, a target that has been implicated with breast cancer.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div id="description-tab" class="info-tab"> | ||
+ | <div class="col-lg-12 col-md-12"> | ||
+ | <h2>Other Description</h2> | ||
+ | <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean eget purus nisl. Quisque gravida vestibulum neque, vel ullamcorper erat finibus nec. Quisque vitae consequat eros. Proin finibus hendrerit velit. Duis non fermentum justo. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia Curae; Suspendisse ut iaculis justo. Donec sapien leo, volutpat vitae leo ac, ornare placerat turpis. Aliquam nulla massa, auctor vitae feugiat nec, gravida at mauris. Praesent dignissim mollis tempor. Phasellus nec nisi nisl. Ut tincidunt orci non sollicitudin iaculis. Suspendisse potenti.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <a class="btn" href="https://www.google.ca">Visit Page</a> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="ua-home-aesthetic" class="text-center"> | ||
+ | <div class="overlay"> | ||
+ | <div class="container"> | ||
+ | <h2>Video Here?</h2> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!-- <div id="ua-home-links" class="text-center"> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <h2>Research</h2> | ||
+ | <div class="link-tabs"> | ||
+ | <div class="link-tab col-lg-4 col-md-4 col-sm-6 col-xs-12"><a href="#">Team</a></div> | ||
+ | <div class="link-tab col-lg-4 col-md-4 col-sm-6 col-xs-12"><a href="#">Team</a></div> | ||
+ | <div class="link-tab col-lg-4 col-md-4 col-sm-6 col-xs-12"><a href="#">Team</a></div> | ||
+ | <div class="link-tab col-lg-4 col-md-4 col-sm-6 col-xs-12"><a href="#">Team</a></div> | ||
+ | <div class="link-tab col-lg-4 col-md-4 col-sm-6 col-xs-12"><a href="#">Team</a></div> | ||
+ | <div class="link-tab col-lg-4 col-md-4 col-sm-6 col-xs-12"><a href="#">Team</a></div> | ||
+ | <div class="link-tab col-lg-4 col-md-4 col-sm-6 col-xs-12"><a href="#">Team</a></div> | ||
+ | <div class="link-tab col-lg-4 col-md-4 col-sm-6 col-xs-12"><a href="#">Team</a></div> | ||
+ | <div class="link-tab col-lg-4 col-md-4 col-sm-6 col-xs-12"><a href="#">Team</a></div> | ||
+ | <div class="link-tab col-lg-4 col-md-4 col-sm-6 col-xs-12"><a href="#">Team</a></div> | ||
+ | <div class="link-tab col-lg-4 col-md-4 col-sm-6 col-xs-12"><a href="#">Team</a></div> | ||
+ | <div class="link-tab col-lg-4 col-md-4 col-sm-6 col-xs-12"><a href="#">Team</a></div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> --> | ||
+ | |||
+ | <footer id="ua-footer"> | ||
+ | <div class="container"> | ||
+ | <div class="row"> | ||
+ | <div class="col-lg-9 col-md-9 hidden-sm hidden-xs"> | ||
+ | </div> | ||
+ | <div class="col-lg-3 col-md-3 col-sm-12 col-xs-12" > | ||
+ | <div class="text-center"> | ||
+ | <a class="fa fa-facebook-square fa-2x" aria-hidden="true" href="#"></a> | ||
+ | <a class="fa fa-twitter-square fa-2x" aria-hidden="true" href="#"></a> | ||
+ | <a class="fa fa-instagram fa-2x" aria-hidden="true" href="#"></a> | ||
+ | <a class="fa fa-github fa-2x" aria-hidden="true" href="#"></a> | ||
+ | <h2>igem.ualberta@gmail.com</h2> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </footer> | ||
+ | |||
+ | <script type="text/javascript" src="https://2017.igem.org/Template:UAlberta/JavaScript?action=raw&ctype=text/javascript"></script> | ||
</body> | </body> | ||
</html> | </html> | ||
− |
Revision as of 16:54, 30 August 2017
Abstract
Team UAlberta aims to develop a buoyancy-based screening system for protein-protein interactions in Escherichia coli.
What are the problems being addressed?
What goals are we tying to achieve?
How will we accomplish those goals?
Project Description
Team UAlberta aims to develop a buoyancy-based screening system for protein-protein interactions in Escherichia coli. This system relies on a bacterial two-hybrid system (BACTH) where the reconstitution of the T18 and T25 domains of adenylate cyclase, due to the interaction between two proteins fused to the two domains, will restore the activity of the adenylate cyclase depending on the strength of the protein-protein interaction. Reconstitution of adenylate cyclase restores the production of cAMP, which will then upregulate a cluster of genes controlling gas vesicle production, therefore conferring buoyancy to E. coli cells. Thus, the observed buoyancy in bacteria is ultimately dependent on the strength of the protein-protein interaction. The purpose of this system is to utilize buoyancy to physically separate bacteria expressing successful protein interactions from unsuccessful variants. Team UAlberta will first test and characterize the system using pairs of proteins with well-characterized interactions, such as leucine zippers. After initial testing, Team UAlberta aims to use the system in conjunction with directed evolution to engineer a potent peptide inhibitor of NHERF2, a target that has been implicated with breast cancer.
Other Description
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Aenean eget purus nisl. Quisque gravida vestibulum neque, vel ullamcorper erat finibus nec. Quisque vitae consequat eros. Proin finibus hendrerit velit. Duis non fermentum justo. Vestibulum ante ipsum primis in faucibus orci luctus et ultrices posuere cubilia Curae; Suspendisse ut iaculis justo. Donec sapien leo, volutpat vitae leo ac, ornare placerat turpis. Aliquam nulla massa, auctor vitae feugiat nec, gravida at mauris. Praesent dignissim mollis tempor. Phasellus nec nisi nisl. Ut tincidunt orci non sollicitudin iaculis. Suspendisse potenti.