Revision as of 22:33, 1 November 2017

For the OR2W1 in pSB1C3 plasmid, one reaction used the BglII and PstI enzymes. The expected products for this reaction were two bands at 2.3kb and 0.8kb. Because the BglII site is unique to OR2W1 coding sequence, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. The second reaction used the MscI enzyme. The expected products for this reaction were also two bands at 1.9kb and 1.2kb. Because both the pSB1C3 plasmid and OR10G4 coding sequence each contain a single MscI site, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. As shown by the image of the gel with the restriction endonuclease reaction products, the band patterns reveal a colony containing the pSB1C3 plasmid with the OR2W1 coding sequence.

This is an OR sequence that responds to hexanol.

For the OR10G4 in pSB1C3 plasmid, one reaction used the EcoRI and BamHI enzymes. The expected products for this reaction were two bands at 2.3kb and 0.7kb. Because the BamHI site is unique to OR10G4 coding sequence, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. The second reaction used the BlpI and PstI enzymes. The expected products for this reaction were also two bands at 2.3kb and 0.7kb. Because the BlpI site is unique to OR10G4 coding sequence, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. As shown by the image of the gel with the restriction endonuclease reaction products, the band patterns reveal a colony contains the pSB1C3 plasmid with the OR10G4 coding sequence.

TEL: 845.938.3915

@@ Line 840: / Line 840: @@
 	<div id="vbid-b34e5300-r2wbnbrn" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_BODY">
-		<p class="font_7" style="text-align: justify;">Human olfactory receptor, family 2, subfamily W, member 1 (OR2W1). Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) that share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome.<br></p><p class="font_7" style="text-align: justify;"><br></p><p class="font_7" style="text-align: justify;">This is an OR sequence that responds to hexanol.<br></p><p><br></p>
+		<p class="font_7" style="text-align: justify;">For the OR2W1 in pSB1C3 plasmid, one reaction used the BglII and PstI enzymes. The expected products for this reaction were two bands at 2.3kb and 0.8kb. Because the BglII site is unique to OR2W1 coding sequence, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. The second reaction used the MscI enzyme. The expected products for this reaction were also two bands at 1.9kb and 1.2kb. Because both the pSB1C3 plasmid and OR10G4 coding sequence each contain a single MscI site, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. As shown by the image of the gel with the restriction endonuclease reaction products, the band patterns reveal a colony containing the pSB1C3 plasmid with the OR2W1 coding sequence.<br></p><p class="font_7" style="text-align: justify;"><br></p><p class="font_7" style="text-align: justify;">This is an OR sequence that responds to hexanol.<br></p><p><br></p>
 	</div>
@@ Line 1,010: / Line 1,010: @@
 	<div id="vbid-b34e5300-jfpmjajc" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu   allow-mobile-hide" data-menu-name="PREVIEW_BODY">
-		<p>Human olfactory receptor, family 10, subfamily G, member 4 (OR10G4). Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) that share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome.</p><p><br></p><p>This is an OR sequence that responds to vanillin.<br></p><p><br></p>
+		<p>For the OR10G4 in pSB1C3 plasmid, one reaction used the EcoRI and BamHI enzymes. The expected products for this reaction were two bands at 2.3kb and 0.7kb. Because the BamHI site is unique to OR10G4 coding sequence, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. The second reaction used the BlpI and PstI enzymes. The expected products for this reaction were also two bands at 2.3kb and 0.7kb. Because the BlpI site is unique to OR10G4 coding sequence, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. As shown by the image of the gel with the restriction endonuclease reaction products, the band patterns reveal a colony contains the pSB1C3 plasmid with the OR10G4 coding sequence.<br></p><p><br></p>
 	</div>

Difference between revisions of "Team:USMA-West Point/Basic Part"

Revision as of 22:33, 1 November 2017

USMA-West_Point

USMA-WEST POINT

Basic Parts

OR2W1 in pSB1C3

Part:BBa_K2222001

OR10G4 in pSB1C3

Part:BBa_K2222000

DEPARTMENT OF CHEMISTRY AND LIFE SCIENCE

BARTLETT HALL, ROOM 400, WEST POINT, NY 10996, USA