Difference between revisions of "Team:USMA-West Point/Basic Part"

 
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<h2 id="vbid-7d38c6a1-8ye3bio7" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >usMA-WEST POINT<br></h2>
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<span id="element-78132adefe1a4d4"  class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Basic Parts</span>
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<span id="element-78132adefe1a4d4"  class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Parts</span>
 
 
 
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<p class="font_7" style="text-align: justify;">Human olfactory receptor, family 2, subfamily W, member 1 (OR2W1). Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) that share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome.<br></p><p class="font_7" style="text-align: justify;"><br></p><p class="font_7" style="text-align: justify;">This is an OR sequence that responds to hexanol.<br></p><p><br></p>
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<p class="font_7" style="text-align: justify;">For the OR2W1 in pSB1C3 plasmid, one reaction used the BglII and PstI enzymes. The expected products for this reaction were two bands at 2.3kb and 0.8kb. Because the BglII site is unique to OR2W1 coding sequence, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. The second reaction used the MscI enzyme. The expected products for this reaction were also two bands at 1.9kb and 1.2kb. Because both the pSB1C3 plasmid and OR10G4 coding sequence each contain a single MscI site, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. As shown by the image of the gel with the restriction endonuclease reaction products, the band patterns reveal a colony containing the pSB1C3 plasmid with the OR2W1 coding sequence.<br></p><p class="font_7" style="text-align: justify;"><br></p><p class="font_7" style="text-align: justify;"><br></p><p><br></p>
 
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<p>Human olfactory receptor, family 10, subfamily G, member 4 (OR10G4). Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) that share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome.</p><p><br></p><p>This is an OR sequence that responds to vanillin.<br></p><p><br></p>
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<p>For the OR10G4 in pSB1C3 plasmid, one reaction used the EcoRI and BamHI enzymes. The expected products for this reaction were two bands at 2.3kb and 0.7kb. Because the BamHI site is unique to OR10G4 coding sequence, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. The second reaction used the BlpI and PstI enzymes. The expected products for this reaction were also two bands at 2.3kb and 0.7kb. Because the BlpI site is unique to OR10G4 coding sequence, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. As shown by the image of the gel with the restriction endonuclease reaction products, the band patterns reveal a colony contains the pSB1C3 plasmid with the OR10G4 coding sequence.<br></p><p><br></p>
 
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Latest revision as of 23:23, 1 November 2017

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USMA-West_Point


About - iGem 01

USMA-WEST POINT


Basic Parts



OR2W1 in pSB1C3



Part:BBa_K2222001



For the OR2W1 in pSB1C3 plasmid, one reaction used the BglII and PstI enzymes. The expected products for this reaction were two bands at 2.3kb and 0.8kb. Because the BglII site is unique to OR2W1 coding sequence, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. The second reaction used the MscI enzyme. The expected products for this reaction were also two bands at 1.9kb and 1.2kb. Because both the pSB1C3 plasmid and OR10G4 coding sequence each contain a single MscI site, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. As shown by the image of the gel with the restriction endonuclease reaction products, the band patterns reveal a colony containing the pSB1C3 plasmid with the OR2W1 coding sequence.






OR10G4 in pSB1C3



Part:BBa_K2222000



For the OR10G4 in pSB1C3 plasmid, one reaction used the EcoRI and BamHI enzymes. The expected products for this reaction were two bands at 2.3kb and 0.7kb. Because the BamHI site is unique to OR10G4 coding sequence, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. The second reaction used the BlpI and PstI enzymes. The expected products for this reaction were also two bands at 2.3kb and 0.7kb. Because the BlpI site is unique to OR10G4 coding sequence, then only a single band at 2.2kb would be expected if the colony contained only the empty pSB1C3 plasmid. As shown by the image of the gel with the restriction endonuclease reaction products, the band patterns reveal a colony contains the pSB1C3 plasmid with the OR10G4 coding sequence.




DEPARTMENT OF CHEMISTRY AND LIFE SCIENCE



BARTLETT HALL, ROOM 400, WEST POINT, NY 10996, USA


TEL: 845.938.3915