Difference between revisions of "Team:USMA-West Point/Demonstrate"

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<h1>Demonstrate</h1>
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<h3>Gold Medal Criterion #4</h3>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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<h4> What should we do for our demonstration?</h4>
 
  
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<h5> Standard teams </h5>
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
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<h5> Special track teams </h5>
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<p><span style="font-family: &quot;open sans&quot;, sans-serif; font-size: 16px;">Here dissected chicken dorsal root ganglia (DRGs) were nucleofected with plasmid OR2W1. The neurons were infiltrated with  which in the presence of calcium fluoresces. During an action potential, calcium enters the cell during depolarization which occurs when the ligand binds to the OR2W1 receptors. The change in fluorescence demonstrates the function of this device.</span></p>
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-47ddf2a4-vfvbl1q1" data-preview-style="style-47ddf2a4-a8ududu2" data-style="style-7f715-7lb5zosuoo" data-orig-thumb-height="1753" data-orig-thumb-width="895" data-vbid="vbid-47ddf2a4-vfvbl1q1" data-bgimg="https://lh3.googleusercontent.com/YMfGaZKgomwnf7oAeYuS1_W6UM84jfV7OMiqKW0y9u4QCFPrmHRkk8sERj0e7W3y9NuLEKgl4kdb0qnq">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-47ddf2a4-vr71v3pf-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
 +
<a class="image-link top-layer not-wrapping" href="/vbid-47ddf2a4-pa19imep"  data-link-type="LIGHTBOX" target="_self" ></a>
  
 +
<div  id="vbid-47ddf2a4-vr71v3pf" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/YMfGaZKgomwnf7oAeYuS1_W6UM84jfV7OMiqKW0y9u4QCFPrmHRkk8sERj0e7W3y9NuLEKgl4kdb0qnq=s300);"  data-orig-width="895" data-orig-height="1753" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 
</div>
 
</div>
 +
  
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 +
 +
<div class="preview-form order-handle">
 +
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-47ddf2a4-w6eyskhc"  class="sub item-box  page-box style-47ddf2a4-a8ududu2          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-47ddf2a4-a8ududu2" data-preview-styleid='style-47ddf2a4-a8ududu2' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-47ddf2a4-w6eyskhc" data-preview-style="style-47ddf2a4-a8ududu2" data-style="style-7f715-7yhglr7kca" data-orig-thumb-height="1668" data-orig-thumb-width="895" data-vbid="vbid-47ddf2a4-w6eyskhc" data-bgimg="https://lh3.googleusercontent.com/Y-rfj9DAeV32fl82do8Y4zWj710Esg-fa6wOT4x54WfPq5m7uzdCqY5zzz2y8TBArwPV14rrp-O6cGKk">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-47ddf2a4-6pun72q7-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<a class="image-link top-layer not-wrapping" href="/vbid-47ddf2a4-lbtcwrw9"  data-link-type="LIGHTBOX" target="_self" ></a>
 +
 +
<div  id="vbid-47ddf2a4-6pun72q7" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/Y-rfj9DAeV32fl82do8Y4zWj710Esg-fa6wOT4x54WfPq5m7uzdCqY5zzz2y8TBArwPV14rrp-O6cGKk=s300);"  data-orig-width="895" data-orig-height="1668" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 +
 +
<div class="preview-form order-handle">
 +
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-47ddf2a4-3agw4z4z"  class="sub item-box  page-box style-47ddf2a4-a8ududu2          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-47ddf2a4-a8ududu2" data-preview-styleid='style-47ddf2a4-a8ududu2' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-47ddf2a4-3agw4z4z" data-preview-style="style-47ddf2a4-a8ududu2" data-style="style-7f715-nmxoztwtzr" data-orig-thumb-height="1742" data-orig-thumb-width="867" data-vbid="vbid-47ddf2a4-3agw4z4z" data-bgimg="https://lh3.googleusercontent.com/1tOaKl-kRyfikqOIFRwlWoRncfaM3wqxWVKETLFgrsmN9Glcoj-Zd2wk6kDUhHNP4qZhsSXPoVLwETuqoBo">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-47ddf2a4-y2ojlb72-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<a class="image-link top-layer not-wrapping" href="/vbid-47ddf2a4-pa19imep"  data-link-type="LIGHTBOX" target="_self" ></a>
 +
 +
<div  id="vbid-47ddf2a4-y2ojlb72" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/1tOaKl-kRyfikqOIFRwlWoRncfaM3wqxWVKETLFgrsmN9Glcoj-Zd2wk6kDUhHNP4qZhsSXPoVLwETuqoBo=s300);"  data-orig-width="867" data-orig-height="1742" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 +
 +
<div class="preview-form order-handle">
 +
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
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 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-47ddf2a4-tkqswdku"  class="sub item-box  page-box style-47ddf2a4-a8ududu2          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-47ddf2a4-a8ududu2" data-preview-styleid='style-47ddf2a4-a8ududu2' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-47ddf2a4-tkqswdku" data-preview-style="style-47ddf2a4-a8ududu2" data-style="style-7f715-6fzkkkwai3" data-orig-thumb-height="1191" data-orig-thumb-width="855" data-vbid="vbid-47ddf2a4-tkqswdku" data-bgimg="https://lh3.googleusercontent.com/lunI1RYPeLAcbDP3HkmQO9DOqISZwX4ff3WJkbT3EcbQCaDjlybVA5cR69IPm2k6wZhK2-sGrp7behW8jg">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-47ddf2a4-askoytqy-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<a class="image-link top-layer not-wrapping" href="/vbid-47ddf2a4-pa19imep"  data-link-type="LIGHTBOX" target="_self" ></a>
 +
 +
<div  id="vbid-47ddf2a4-askoytqy" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/lunI1RYPeLAcbDP3HkmQO9DOqISZwX4ff3WJkbT3EcbQCaDjlybVA5cR69IPm2k6wZhK2-sGrp7behW8jg=s300);"  data-orig-width="855" data-orig-height="1191" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 +
 +
<div class="preview-form order-handle">
 +
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- GALLERIES END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-0d980238-qqcb9mt2"  class="master item-box  gallery-box style-0d980238-6rkb49z5    button-effects btn_hover3      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-0d980238-6rkb49z5" data-preview-styleid='style-0d980238-6rkb49z5' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-0d980238-6rkb49z5 content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-0d980238-6rkb49z5" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-0d980238-qqcb9mt2" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-0d980238-6rkb49z5" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/jpruneski8"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-0d980238-3icuax6a"  class="sub item-box  page-box style-0d980238-6rkb49z5          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-0d980238-6rkb49z5" data-preview-styleid='style-0d980238-6rkb49z5' data-preset-type-id="UNRESOLVED">
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Demonstrate


Demonstrate



Here dissected chicken dorsal root ganglia (DRGs) were nucleofected with plasmid OR2W1. The neurons were infiltrated with which in the presence of calcium fluoresces. During an action potential, calcium enters the cell during depolarization which occurs when the ligand binds to the OR2W1 receptors. The change in fluorescence demonstrates the function of this device.




( by USMA-West Point iGEM Team (ANM, MM, JP, JKW ©