Difference between revisions of "Team:USMA-West Point/Demonstrate"

 
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<h1>Demonstrate</h1>
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<h3>Gold Medal Criterion #4</h3>
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<a href="https://2017.igem.org/Team:USMA-West_Point" data-link-type="EXISTING"  target="_self" >
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<br />
  
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve a their gold medals!
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Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.
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If you have built a proof of concept system, you can demonstrate it working under real world conditions. If you have built a biological device that is intended to be a sensor, can you show it detecting whatever it is intended to sense. If it is intended to work in the field, you can show how this might work using a simulated version in the lab, or a simulation of your device in the field.<strong> Please note biological materials must not be taken out of the lab</strong>.
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<p class="font_7" style="text-align: center; line-height: 1.5em;"><span style="background-color:#000000;">Here dissected chicken dorsal root ganglia (DRGs) were nucleofected with plasmid OR2W1. The neurons were infiltrated with which in the presence of calcium fluoresces. During an action potential, calcium enters the cell during depolarization which occurs when the ligand binds to the OR2W1 receptors. The change in fluorescence demonstrates the function of this device.</span><br></p>
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Special track teams can achieve this medal criterion by bringing their work to the Jamboree and showcasing it in the track event. Art & Design, Measurement, Hardware and Software tracks will all have showcase events at the Giant Jamboree.<strong> Please note biological materials must not be taken out of the lab</strong>.
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USMA-West_Point

DEMONSTRATE - iGem 01

usMA-WEST POINT


demonstrate



Here dissected chicken dorsal root ganglia (DRGs) were nucleofected with plasmid OR2W1. The neurons were infiltrated with which in the presence of calcium fluoresces. During an action potential, calcium enters the cell during depolarization which occurs when the ligand binds to the OR2W1 receptors. The change in fluorescence demonstrates the function of this device.



Fluorescent Calcium Imaging



DEPARTMENT OF CHEMISTRY AND LIFE SCIENCE



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