Difference between revisions of "Team:USMA-West Point/Design"

(Began Bioreactor and Fluidics Design)
 
(12 intermediate revisions by 4 users not shown)
Line 1: Line 1:
 
{{USMA-West_Point}}
 
{{USMA-West_Point}}
−
<html>
 
  
  
 +
<html xmlns="http://www.w3.org/1999/xhtml">
 +
<head><meta name='robots' content='noindex, nofollow'>
 +
<title>
 +
Design - iGem 01</title>
 +
<!-- META -->
 +
<meta charset="UTF-8">
 +
<meta name="viewport" content="width=device-width, maximum-scale=1"/>
 +
 +
 +
 +
 +
<script src="https://www.youtube.com/iframe_api"></script>
 +
<!-- CSS -->
 +
<link rel="stylesheet" href="//www.imcreator.com/css/common.css?v=1.4.1f">
 +
<link rel="stylesheet" type="text/css" href="//www.imcreator.com/all_css.css?v=1.4.1f" />
  
 +
 +
 +
<link rel="icon" type="image/png" href="https://lh3.googleusercontent.com/dd_e0xA19up9208Tv6odcjHEw6z4cKAA6fTgjZ9ynkKoSnr5R4vFxI7gZp6pnZH1Vi5T5f-fphjFsrLG=s30" />
 +
<link rel="apple-touch-icon" href="https://lh3.googleusercontent.com/dd_e0xA19up9208Tv6odcjHEw6z4cKAA6fTgjZ9ynkKoSnr5R4vFxI7gZp6pnZH1Vi5T5f-fphjFsrLG=s30" />
 +
 +
 +
 +
<link rel="stylesheet" type="text/css" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css" />
 +
 +
<link id="vbid-beedfe5c-txbf9ali-STRIPE_DATA" rel="stylesheet" type="text/css"  href="//www.imcreator.com/stripe_data_css?page_id=vbid-beedfe5c-txbf9ali-STRIPE_DATA&v=1.4.1f">
 +
 +
<link id="vbid-7d38c6a1-ojqdlra3-STRIPE_DATA" rel="stylesheet" type="text/css"  href="//www.imcreator.com/stripe_data_css?page_id=vbid-7d38c6a1-ojqdlra3-STRIPE_DATA&v=1.4.1f">
 +
 +
<link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/fonts.css?v=1.4.1f" />
 +
<link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/effects.css?v=1.4.1f" />
 +
<link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/lightbox.css?v=1.4.1f">
 +
 +
<link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/previewhelper.css?v=1.4.1f">
 +
 +
<link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/spimeview.css?v=1.4.1f">
 +
 +
<link id="vbid-beedfe5c-txbf9ali-STATIC_STYLE" rel="stylesheet" type="text/css" href="//www.imcreator.com/static_style?v=1.4.1f&vbid=vbid-beedfe5c-txbf9ali&caller=static">
 +
 +
 +
<!-- SCRIPT -->
 +
<script src="https://code.jquery.com/jquery-2.x-git.min.js" type="text/javascript"></script>
 +
<script src="https://maps.googleapis.com/maps/api/js?key=AIzaSyByWRZWFbQRxJM7SiNz2NURPDPJPqTtBQQ" type="text/javascript"></script>
 +
 +
<script src="//www.imcreator.com/js/xprs_helper.js?v=1.4.1f" type="text/javascript"></script>
 +
 +
<script src="https://imos004-dot-im--os.appspot.com/js/imos.js?v=1.4.1f" type="text/javascript"></script>
  
−
<div class="column full_size">
+
<script type="text/javascript" src="//www.imcreator.com/all_js.js?v=1.4.1f"></script>
−
<h1>Design</h1>
+
<script src="//www.imcreator.com/js/lib/touchswipe/jquery.mobile.custom.min.js"></script>
−
<p>
+
<script type="text/javascript">IMOS.pageView();</script>
−
Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
+
−
</p>
+
 +
 +
 +
<script src="//www.imcreator.com/js/preview_helper.js?v=1.4.1f"  type="text/javascript"></script>
 +
 +
 +
<!-- User analytics -->
  
−
<p>
 
−
This page is different to the "Applied Design Award" page. Please see the <a href="https://2017.igem.org/Team:USMA-West_Point/Applied_Design">Applied Design</a> page for more information on how to compete for that award.
 
−
</p>
 
  
 +
<!-- Serving analytics -->
 +
<script>
 +
var currentDomain =  window.location.host;
 +
if (currentDomain != "localhost:7000"){
 +
var _gaq = _gaq || [];
 +
_gaq.push(['_setAccount', 'UA-58695071-1']);
 +
_gaq.push(['_setDomainName', currentDomain]);
 +
_gaq.push(['_setAllowLinker', true]);
 +
_gaq.push(['_trackPageview']);
 +
}
 +
</script>
 +
 +
<!-- Label config-->
 +
<script>
 +
 +
var LABEL_CONFIG = {};
 +
 +
</script></head>
 +
 +
<body class="fast-animated-bg pattern-bg " data-ecommerce-solution="NONE" data-root-id="vbid-7d38c6a1-ojqdlra3" data-root-style-id="style-7d38c6a1-d2bgnenh" data-default-currency="USD" data-osid="osid--ec2fa157-ff75c294"  data-app-version="1.4.1f" data-caller="static"  data-ecommerce-dashboard="https://dashboard.shoprocket.co" data-static-server="//www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
 +
 +
<div id="xprs" data-website-name="iGem 01" class="xprs-holder" >
 +
<div class="main-page "  >
 +
<div id="content">
 +
<div  id="vbid-beedfe5c-txbf9ali"  class="master container style-beedfe5c-gjb1vmqo content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="Design"  data-itemslug="design" data-itemstyleid="style-beedfe5c-gjb1vmqo" data-margintop="" data-arranger="stripes" data-layout="multi" data-vbid="vbid-beedfe5c-txbf9ali" data-preset-type-id="ITEMS" data-preview-style="style-beedfe5c-gjb1vmqo" data-style="style-7f715-fyu5oogf7j" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="stripes"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="false"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT=""
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
 +
<!-- MENUS START -->
 +
<div  id="vbid-7d38c6a1-8otprari"  class="master item-box  header-box style-7d38c6a1-8jo8o4ao injected        " data-holder-type="header"" data-holder-type="header"  data-child-type="ORIGIN"  data-styleid="style-7d38c6a1-8jo8o4ao" data-preview-styleid='style-7d38c6a1-8jo8o4ao' data-preset-type-id="MENUS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="header-wrapper item-wrapper menus-wrapper ">
 +
 +
<div class="item-content leaf menu_layout header content" data-preview-style="style-7d38c6a1-8jo8o4ao" data-style="style-7f715-scjjarqgxu"  data-behavior-type="NOTHING" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-7d38c6a1-8otprari" data-bgimg="">
 +
<div class="preview-content-wrapper">
 +
<div class="preview-content-holder">
 +
<div class="left-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<div class="logo-holder">
 +
<!-- ICON TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
</div>
 +
<div class="helper-div">
 +
<div class="item-details menu">
 +
<!-- TITLE TEMPLATE -->
 +
 +
<div class="preview-title-holder removable-parent">
 +
 +
<a href="https://2017.igem.org/Team:USMA-West_Point" data-link-type="EXISTING"  target="_self" >
 +
 +
<h2 id="vbid-7d38c6a1-8ye3bio7" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >usMA-WEST POINT<br></h2>
 +
</a>
 +
</div>
 +
<br />
 +
 +
<!-- SUBTITLE TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
</div>
 +
<div class="right-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<!-- LINKS TEMPLATE -->
 +
<div class="preview-item-links ">
 +
<ul class="preview-links-wrapper">
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-7d38c6a1-0vahew5d"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Home</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
 +
 +
 +
<li>
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/vbid-f02482b7-t3dtwkay" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="c4532978"  class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Team</span>
 +
</a>
 +
<ul class="submenu">
 +
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Team" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="e14b181a" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Students, Advisors, PIs, and Instructors</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Collaborations" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="facc214a" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Collaboration</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
 +
</ul>
 +
</li class="removable-parent">
 +
 +
 +
 +
<li>
 +
 +
<span id="element-4cd1f662a476dc8"  class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Project</span>
 +
 +
<ul class="submenu">
 +
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Project" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="38c7042b" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Project</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Description" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="42d444bb" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Description</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Design" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="59bf92c2" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Design</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Experiments" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="487700ca" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Experiments</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Notebook" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="ab39f1bd" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Notebook</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/InterLab" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="21fa8523" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >InterLab Study</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Results" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="cff7eeb1" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Results</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Demonstrate" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="6157824b" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Demonstrate</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Attributions" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="a0b12bde" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Attribution</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<!-- [] -->
 +
</ul>
 +
</li>
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Deliverables" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="09c40e1e"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Deliverables</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
 +
 +
 +
<li>
 +
 +
<span id="element-78132adefe1a4d4"  class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Parts</span>
 +
 +
<ul class="submenu">
 +
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Parts" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="90d18fdb" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Parts</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Basic_Part" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="fa2fa5ec" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Basic Parts</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Part_Collection" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="37cd96f3" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Parts Collection</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<!-- [] -->
 +
</ul>
 +
</li>
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Safety" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="99ad6039"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
 +
 +
 +
<li>
 +
 +
<span id="element-b165365db185e95"  class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Human Practices </span>
 +
 +
<ul class="submenu">
 +
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/HP/Gold_Integrated" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="61c8e27f" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Integrated and Gold</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Engagement" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="f9e986dd" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Public Engagement</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
 +
</ul>
 +
</li class="removable-parent">
 +
 +
 +
 +
<li>
 +
 +
<span id="element-905a6c68c42aebb"  class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Awards</span>
 +
 +
<ul class="submenu">
 +
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Entrepreneurship" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="ca6cd864" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Entreneurship</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Applied_Design" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="bc7909bf" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Applied Design</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="https://2017.igem.org/Team:USMA-West_Point/Hardware" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="c5142c05" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Hardware</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
<!-- [1] -->
 +
</ul>
 +
</li>
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</ul>
 
</div>
 
</div>
 +
 +
 +
<button class="hamburger links-menu-btn  small" type="button">
 +
  <span class="hamburger-box">
 +
  <span class="hamburger-inner"></span>
 +
  </span>
 +
</button>
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="menu"
 +
data-MENU_OVERLAY="relative"
 +
data-MENU_SCROLL="true"
 +
data-ALWAYS_MINIFIED="false"
 +
data-MENU_POSITION="top"
 +
data-MENU_ALIGN="left"
 +
data-BACKGROUND_COLOR="rgb(201, 151, 0)"
 +
></div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- MENUS END -->
 +
 +
 +
 +
 +
<!-- PROMO START -->
 +
<div  id="vbid-beedfe5c-izcx32dg"  class="master item-box  gallery-box style-beedfe5c-okqhupa7    button-effects btn_hover3  items-enter-effects item_enter2    " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-beedfe5c-okqhupa7" data-preview-styleid='style-beedfe5c-okqhupa7' data-preset-type-id="PROMO">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper promo-wrapper ">
 +
 +
<div  class="sub container style-beedfe5c-okqhupa7 content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-beedfe5c-okqhupa7" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-beedfe5c-izcx32dg" data-preset-type-id="PROMO" data-preview-style="style-beedfe5c-okqhupa7" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-beedfe5c-ndocsvg9"  class="sub item-box  page-box style-beedfe5c-okqhupa7          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-beedfe5c-okqhupa7" data-preview-styleid='style-beedfe5c-okqhupa7' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-beedfe5c-ndocsvg9" data-preview-style="style-beedfe5c-okqhupa7" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="814" data-orig-thumb-width="3264" data-vbid="vbid-beedfe5c-ndocsvg9" data-bgimg="https://lh3.googleusercontent.com/Y_fHvvRrbYHtoqH1YU5OR8UrnViHDlVEXPdZ55NnjysVmgKGC13On5nKWAGPcBTgedmmNUL0dZY9MJ8NnA">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-beedfe5c-lgw88smf-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<div class="column half_size">
+
<div id="vbid-beedfe5c-lgw88smf" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/Y_fHvvRrbYHtoqH1YU5OR8UrnViHDlVEXPdZ55NnjysVmgKGC13On5nKWAGPcBTgedmmNUL0dZY9MJ8NnA=s300);"  data-orig-width="3264" data-orig-height="814" >
−
<h5>What should this page contain?</h5>
+
<!-- VIDEO TEMPLATE -->
−
<ul>
+
−
<li>Explanation of the engineering principles your team used in your design</li>
+
−
<li>Discussion of the design iterations your team went through</li>
+
−
<li>Experimental plan to test your designs</li>
+
−
</ul>
+
  
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 
</div>
 
</div>
 +
  
−
<div class="column half_size">
+
</div>
−
<h5>Inspiration</h5>
+
</div>
−
<ul>
+
<div class="text-side shrinker-parent">
−
<li><a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">2016 MIT</a></li>
+
<div class="vertical-aligner">
−
<li><a href="https://2016.igem.org/Team:BostonU/Proof">2016 BostonU</a></li>
+
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
−
<li><a href="https://2016.igem.org/Team:NCTU_Formosa/Design">2016 NCTU Formosa</a></li>
+
<div class="draggable-div-holder"></div>
−
</ul>
+
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h1 id="vbid-beedfe5c-vaaud8o9" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Design<br></h1>
 +
 
</div>
 
</div>
 +
<br class="lower-line-break" />
  
−
<h3>Bioreactor and Fluidics Design</h3>
+
−
<p>
+
−
Following the successes at West Point with Axion's Muse, a new challenge was brought upon to conduct the same tests but in a more economic fashion. We decided to engineer a similar system with the bioreactor as the main focus. The bioreactor is printed from FormLabs Form 2 printer using a non-cytotoxic Dental SG resin. We designed the bioreactor to allow for neurons to be cultured on a mulitelectrode array (MEA), to measure activity. The various ports allow media to flow through the system as well as the injection of the olfactant.
+
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-beedfe5c-d05vgqwq" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="font_7" style="text-align: center; line-height: 1.5em;"><span style="background-color:#000000;">The team has provided two sequences to produce odorant receptors in animal neurons that have been read on two different devices using fluorescence or field potential measurements and can be utilized with static or dynamic devices. This vision allows us to not only use receptors from the human genome but use receptors across the complete animal kingdom producing a device that expands upon any species’ current method of odorant detection. This vision allows us to identify all smells that have been selected throughout the entire animal kingdom and does not confusion perception with sensation. Better understanding this technology will allow the team to model specific odorant receptors that are presently unknown to detect odorants that currently do not have odorant receptors and are therefore “undetectable”.</span><br></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
  
−
The fluidics component relies on a pressure system to maintain flow of media. Because the entire design is a closed system, pressure changes in one area will transmit to fluid flow, be it air or media. We utilize this property to ensure movement of media from the media reservoir to the waste without waste media cycling back through, instead the air is cycled. We also include the use of syringes and luer locks to facilitate any change in pressure, i.e. adding olfactant, by using them for pressure relief.   
+
−
</p>
+
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- PROMO END -->
 +
 +
 +
 +
 +
<!-- PROJECTS START -->
 +
<div  id="vbid-ad696b0e-rsuknvea"  class="master item-box  gallery-box style-ad696b0e-k2kudjdw          " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="NATIVE_ORDER"  data-styleid="style-ad696b0e-k2kudjdw" data-preview-styleid='style-ad696b0e-k2kudjdw' data-preset-type-id="PROJECTS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper projects-wrapper ">
 +
 +
<div  class="sub container style-ad696b0e-k2kudjdw content matrix  " data-itemtype="folder" data-creator="" data-itemname="LOADING"  data-itemslug="loading" data-itemstyleid="style-ad696b0e-k2kudjdw" data-margintop="" data-arranger="matrix" data-layout="multi" data-vbid="vbid-ad696b0e-rsuknvea" data-preset-type-id="PROJECTS" data-preview-style="style-ad696b0e-k2kudjdw" data-style="style-8ec4c-t1avbp6mmi" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="matrix"
 +
data-ARRANGER_COLS="2"
 +
data-ARRANGER_ITEM_MAX_WIDTH="1000"
 +
data-ARRANGER_ITEM_MIN_WIDTH="338.8"
 +
data-ARRANGER_ITEM_RATIO="1.0"
 +
data-ARRANGER_ITEM_SPACING="15"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="false"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT=""
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-ad696b0e-yc2zcu83"  class="sub item-box  page-box style-ad696b0e-k2kudjdw          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-ad696b0e-k2kudjdw" data-preview-styleid='style-ad696b0e-k2kudjdw' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-ad696b0e-yc2zcu83" data-preview-style="style-ad696b0e-k2kudjdw" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="1018" data-orig-thumb-width="1086" data-vbid="vbid-ad696b0e-yc2zcu83" data-bgimg="https://lh3.googleusercontent.com/NVLZPm-yLNdtvNRx-wIDOnLc-Vk6Yi73dgRNnUmynMJVdq9mq8Fr8RMW-eq54EVrNvnXsMofOUoHBXGpZUE">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div bottom-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-ad696b0e-mwktthqy-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 
 +
<div  id="vbid-ad696b0e-mwktthqy" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/NVLZPm-yLNdtvNRx-wIDOnLc-Vk6Yi73dgRNnUmynMJVdq9mq8Fr8RMW-eq54EVrNvnXsMofOUoHBXGpZUE=s300);"  data-orig-width="1086" data-orig-height="1018" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 
 +
 
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 
 +
</div>
 +
 +
</div>
 +
 +
 
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-subtitle-holder removable-parent order-handle">
 +
 +
<h3 id="element-1de54877194bb68" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Plasmid Design<br></h3>
 +
 
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-ad696b0e-x1mncu9i" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="font_8" style="text-align: justify; font-size: 15px;"><span style="color: rgb(0, 0, 0);">Plasmid design and development is a key component to the eNose Project. Here, we look at the gPCR found in the olfactory system responsible for detecting the sense of smell. Specifically, olfactory neurons contain receptor proteins known as G protein-coupled receptors that recognize when a specific odorant ligand is present causing the generation of an action potential from the cell that is easily measurable with current cell physiology tools and photonic systems.</span><br></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 
 +
 +
<div class="preview-form order-handle">
 +
 
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 
 +
 
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-ad696b0e-cvrt4b16"  class="sub item-box  page-box style-ad696b0e-k2kudjdw          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-ad696b0e-k2kudjdw" data-preview-styleid='style-ad696b0e-k2kudjdw' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-ad696b0e-cvrt4b16" data-preview-style="style-ad696b0e-k2kudjdw" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="701" data-orig-thumb-width="1219" data-vbid="vbid-ad696b0e-cvrt4b16" data-bgimg="https://lh3.googleusercontent.com/3ADLEkdQv_SEHO34n2m1NqG30xCoLyVsvoBigCiKXPVB_6mfESJS_Z4GZStxBE_LlDMk13FEuXq10KYXLxw">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div bottom-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-ad696b0e-zs9d1939-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 
 +
<div  id="vbid-ad696b0e-zs9d1939" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/3ADLEkdQv_SEHO34n2m1NqG30xCoLyVsvoBigCiKXPVB_6mfESJS_Z4GZStxBE_LlDMk13FEuXq10KYXLxw=s300);"  data-orig-width="1219" data-orig-height="701" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 
 +
 
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 
 +
</div>
 +
 +
</div>
 +
 +
 
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-subtitle-holder removable-parent order-handle">
 +
 +
<h3 id="element-1206f18bb56ca9d" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Device Design<br></h3>
 +
 
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-ad696b0e-av6di5xz" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="font_8" style="font-size: 15px;"><span style="color: rgb(0, 0, 0);">The team has built two devices using two different sensing mechanisms: 1) a measured intensity change caused by fluorescence due to calcium binding, and 2) a measured voltage change generated by an action potential. An Arduino was programed and developed to measure low level fluorescent intensity from calcium binding compounds. The Arduino system can be read through Bluetooth or connected to a computer making it versatile for multiple applications. Connecting via Bluetooth allows the system to also collect time and location data to enhance detection capabilities. LED also offer an initial warning to alert the user of any potential threats. The second device is a peristaltic flow chamber with inputs and outputs to transport new media and odorant samples. The cells rest on a microelectrode array that measures field potential measurements during the voltage change of a cell action potential, which is a more sensitive and electronic measurement.</span><br></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 
 +
 +
<div class="preview-form order-handle">
 +
 
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 
 +
 
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-ad696b0e-hafjfxbs"  class="sub item-box  page-box style-ad696b0e-k2kudjdw          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-ad696b0e-k2kudjdw" data-preview-styleid='style-ad696b0e-k2kudjdw' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-ad696b0e-hafjfxbs" data-preview-style="style-ad696b0e-k2kudjdw" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="1511" data-orig-thumb-width="852" data-vbid="vbid-ad696b0e-hafjfxbs" data-bgimg="https://lh3.googleusercontent.com/PaFeFbUs3McmXhhmF9SRf0wHYN0RN81c1RmrrQT9mX4ndi2zQVrQenNdZ_CoWVbJeHIZEYI9T3UzsPirDg">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div bottom-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-ad696b0e-a2qaonqs-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 
 +
<div  id="vbid-ad696b0e-a2qaonqs" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/PaFeFbUs3McmXhhmF9SRf0wHYN0RN81c1RmrrQT9mX4ndi2zQVrQenNdZ_CoWVbJeHIZEYI9T3UzsPirDg=s300);"  data-orig-width="852" data-orig-height="1511" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 
 +
 
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 
 +
</div>
 +
 +
</div>
 +
 +
 
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-subtitle-holder removable-parent order-handle">
 +
 +
<h3 id="element-2f80a506aa32ecf" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Hand Held Photosensor Design</h3>
 +
 
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-ad696b0e-aff9jpp8" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="font_8" style="font-size: 15px;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">A hand held photosensor device was designed to measure the change in fluorescence caused by cell depolarization in the presence of calcium binding ion. A the device is small making it portable, light weight, and inexpensive that can be used with other computational systems to analyze odorant mixtures.</span></span></span></p><p class="font_8" style="font-size: 15px;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"><span class="wixGuard">​</span></span></span></span></p><p class="font_8" style="font-size: 15px;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">An Arduino system was utilized as the initial system to measure the voltage change during increased fluorescence caused by depolarization. The Arduino was Bluetooth compatible allowing voltage measurements to transfer to a portable mobile device while also providing location and time. Additionally, the data could be hard wired to a computer providing the change in voltage. </span></span></span></p><p class="font_8" style="font-size: 15px;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"><span class="wixGuard">​</span></span></span></span></p><p class="font_8" style="font-size: 15px;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Coding was used to determine fluorescent intensity and relay the information through a color display and through colored lights allowing the user to know when they were in the presence of a scent.</span></span></span><br></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 
 +
 +
<div class="preview-form order-handle">
 +
 
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 
 +
 
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-ad696b0e-ve1mdof8"  class="sub item-box  page-box style-ad696b0e-k2kudjdw          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-ad696b0e-k2kudjdw" data-preview-styleid='style-ad696b0e-k2kudjdw' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-ad696b0e-ve1mdof8" data-preview-style="style-ad696b0e-k2kudjdw" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="284" data-orig-thumb-width="934" data-vbid="vbid-ad696b0e-ve1mdof8" data-bgimg="https://lh3.googleusercontent.com/Lrt2KBRdlYe91BoNHlOaHUbn3C1b1PWlbWO2Y-Ez3fJV-Wue8eQ0eo2oD48iSEb_XQzvpcuJwasV2E4VoA">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div bottom-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-ad696b0e-ulaohltb-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 
 +
<div  id="vbid-ad696b0e-ulaohltb" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/Lrt2KBRdlYe91BoNHlOaHUbn3C1b1PWlbWO2Y-Ez3fJV-Wue8eQ0eo2oD48iSEb_XQzvpcuJwasV2E4VoA=s300);"  data-orig-width="934" data-orig-height="284" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 
 +
 
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 
 +
</div>
 +
 +
</div>
 +
 +
 
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-subtitle-holder removable-parent order-handle">
 +
 +
<h3 id="element-a76c2523502908a" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Microelectrode Array Bioreactor</h3>
 +
 
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-ad696b0e-dl49dqcv" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="font_8" style="font-size: 15px;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">A bioreactor was designed to create a closed system that would hold the MEA and be able to capture the neurons’ output signal the neurons. Autodesk Inventor was used to render two pieces with ports and internal channels and printed using a FormLabs Form 2 3D printer.</span></span></span></p><p class="font_8" style="font-size: 15px;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"><span class="wixGuard">​</span></span></span></span></p><p class="font_8" style="font-size: 15px;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">The MEA pad  consists of electrodes to which the cells adhere, leads that transport the signal, contact pads that transfer the signal to a circuit board, and a layer of insulation. Due to complications in manufacturing a custom design, we resorted to purchasing prefabricated MEAs. Two different MEA designs were purchased, standard and perforated.</span></span></span></p><p class="font_8" style="font-size: 15px;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"><span class="wixGuard">​</span></span></span></span></p><p class="font_8" style="font-size: 15px;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Since the bioreactor created a closed system, a perfusion system was developed to allow continual media flow keeping the cells alive without exposing them to the atmosphere, avoiding potential bacteria contamination. This was done by using a peristaltic pump which regulated the flow of media through the system. Olfactants were injected onto the cells through a syringe in the vented screw port at the top of the bioreactor. As olfactant was introduced, pressure was kept constant by withdrawing media and air from another syringe on the other vented screw port on the top of the bioreactor.</span></span></span><br></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 
 +
 +
<div class="preview-form order-handle">
 +
 
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 
 +
 
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-e2fbe4a7-6xblhugq"  class="sub item-box  page-box style-ad696b0e-k2kudjdw          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-ad696b0e-k2kudjdw" data-preview-styleid='style-ad696b0e-k2kudjdw' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-e2fbe4a7-6xblhugq" data-preview-style="style-ad696b0e-k2kudjdw" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="662" data-orig-thumb-width="713" data-vbid="vbid-e2fbe4a7-6xblhugq" data-bgimg="https://lh3.googleusercontent.com/QHQyQi9W7YWhFtMSB3_8GPDQlxtMiKaBY5hVIXhZrPPJz9VdLbJS41X1cKGtwpFqeUfsbdjiyZ0aU1z9mg">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div bottom-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-e2fbe4a7-qdpexqq9-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 
 +
<div  id="vbid-e2fbe4a7-qdpexqq9" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/QHQyQi9W7YWhFtMSB3_8GPDQlxtMiKaBY5hVIXhZrPPJz9VdLbJS41X1cKGtwpFqeUfsbdjiyZ0aU1z9mg=s300);"  data-orig-width="713" data-orig-height="662" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 
 +
 
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 
 +
</div>
 +
 +
</div>
 +
 +
 
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-subtitle-holder removable-parent order-handle">
 +
 +
<h3 id="element-fa13db05ad1c598" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Signal Processing<br></h3>
 +
 
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-e2fbe4a7-36qx5g7b" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<span style="color: rgb(0, 0, 0);"></span><p class="font_8" style="font-size: 15px;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">The search for alternative methods to detect libraries of volatile chemicals is an ongoing endeavor, and for the past 20 years improvements to biosensing has enhanced in speed, sensitivity, and cost. However, these methods are still chemically blind resulting in anosmia of several odors that surround us. </span></span></span></p><p class="font_8" style="font-size: 15px;"><br></p><p class="font_8" style="font-size: 15px;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Signal processing will be the method to analyze the action potential of the electrical signal and remove perception from sensation. Measuring the intensity of an action potential can create electrical fingerprints based on voltage intensity and specific olfactory receptor firing in the presence of an analyte. Therefore, removing the animal and leaving the necessary cells can improve detection specificity by measuring action potentials from olfactory receptor binding of a ligand to individual neurons to produce living biosensors that are selectively persistent in a local environment and can detect analytes with novel capabilities.</span></span></span><br></p><span style="color: rgb(0, 0, 0);"></span>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 
 +
 +
<div class="preview-form order-handle">
 +
 
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 
 +
 
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- PROJECTS END -->
 +
 +
 +
 +
 +
<!-- FOOTERS START -->
 +
<div  id="vbid-7d38c6a1-vhgfqwrt"  class="master item-box  gallery-box style-7d38c6a1-sr6fbz1e injected        " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="ORIGIN"  data-styleid="style-7d38c6a1-sr6fbz1e" data-preview-styleid='style-7d38c6a1-sr6fbz1e' data-preset-type-id="FOOTERS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper footers-wrapper ">
 +
 +
<div  class="sub container style-7d38c6a1-sr6fbz1e content flex   " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-7d38c6a1-sr6fbz1e" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-7d38c6a1-vhgfqwrt" data-preset-type-id="FOOTERS" data-preview-style="style-7d38c6a1-sr6fbz1e" data-style="style-f3095-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-7d38c6a1-eqit56l5"  class="sub item-box  page-box style-7d38c6a1-sr6fbz1e          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-7d38c6a1-sr6fbz1e" data-preview-styleid='style-7d38c6a1-sr6fbz1e' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-7d38c6a1-eqit56l5" data-preview-style="style-7d38c6a1-sr6fbz1e" data-style="style-f3095-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-7d38c6a1-eqit56l5" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 
 +
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 
 +
 
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 
 +
</div>
 +
 +
</div>
 +
 +
 
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-7d38c6a1-jkaeeacm" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >DEPARTMENT OF CHEMISTRY AND LIFE SCIENCE<br></h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-subtitle-holder removable-parent order-handle">
 +
 +
<h3 id="vbid-7d38c6a1-1dorqubw" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">BARTLETT HALL, ROOM 400, WEST POINT, NY 10996, USA</h3>
 +
 
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
 +
 +
 +
<div class="preview-item-links order-handle removable-parent" style="display:inline-block;">
 +
 
 +
<span id="vbid-7d38c6a1-ynlsfdhm"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >TEL: 845.938.3915</span>
 +
 
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-social-wrapper removable-parent order-handle"  >
 +
<div id="vbid-7d38c6a1-bdvlps7u" class="preview-element preview-social-holder magic-circle-holder" data-menu-name="PREVIEW_SOCIAL" data-theme="8" >
 +
 +
<div id="FACEBOOK" class='link-entry ' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="FACEBOOK" data-img-url="//www.imcreator.com/images/socialmedia/8facebook.png">
 +
<a class='social-link-url' href="https://www.facebook.com/West-Point-Department-of-Chemistry-and-Life-Science-385389195808/" target='_blank'><img class='preview-link-img ' src="//www.imcreator.com/images/socialmedia/8facebook.png" ></a>
 +
</div>
 +
 +
<div id="TWITTER" class='link-entry ' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="TWITTER" data-img-url="//www.imcreator.com/images/socialmedia/8twitter.png">
 +
<a class='social-link-url' href="https://twitter.com/WestPoint_USMA?ref_src=twsrc%5Egoogle%7Ctwcamp%5Eserp%7Ctwgr%5Eauthor" target='_blank'><img class='preview-link-img ' src="//www.imcreator.com/images/socialmedia/8twitter.png" ></a>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<br class="lower-line-break" />
 +
 
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- FOOTERS END -->
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="light-box-wrapper  space-layer" style="display:none;">
 +
<div class="light-box-image-holder">
 +
<div class="light-box-image animated">
 +
 +
</div>
 +
<div class="lightbox-text-wrapper ">
 +
<div class="lightbox-text-holder animated">
 +
<div class="lightbox-title"></div>
 +
<div class="lightbox-subtitle"></div>
 +
<div id="paginator" style="display:none;">
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
    <img src="https://lh3.googleusercontent.com/EWqW7DEI4kOTRMLjK2-ObFHp-EYBt5apFYZ1LVFAhLtTLjigCRfx5hCCTKbIjIm68VQ00p9twloHJ9w8=s50" class="download-gallery-btn clickable" style="display: none;">
 +
<img src="https://lh3.googleusercontent.com/TgRyMQvJ3_h9RmOnu7AlhIE7NLOOBsRoBounARrs8fQv8HCRPaFtpBneSqJOSZpI6l7He_bAZKN179JBig=s50" class="close-lightbox-btn clickable" style="opacity: 1;">
 +
<img src="https://lh3.googleusercontent.com/43-pXHjwrpmVO8Oean-6BD0uzARvcqUQrpdi7Yw2bxaXwEoP21UdN5kW6Ks9pdOxf7ropMUrh0djgYPwYPU=s50" class="lightbox-arrow lightbox-left clickable top-layer" >
 +
<img src="https://lh3.googleusercontent.com/9rwgVnDglPdPFugSu98fhDmxzjXC9KovZ_7BuHkXPIv6jvg9S96flGnhL_e4y8mIpPpZQstfqEV-WitY=s50" class="lightbox-arrow lightbox-right clickable top-layer" >
 +
</div>
  
 +
</div>
 +
 +
<script src="//www.imcreator.com/js/lightbox.js?v=1.4.1f"  type="text/javascript"></script>
 +
<script src="//www.imcreator.com/js/spimeengine.js?v=1.4.1f"  type="text/javascript"></script>
 +
 +
 +
<!--  SHOPROCKET  ECOMMERCE  -->
 +
<script>
 +
var JSR = jQuery.noConflict();
 +
var $ = jQuery.noConflict();
 +
</script>
 +
 +
<input type="hidden" name="sr-companyid" id="sr-companyid" value="">
 +
</body>
  
 +
 
</html>
 
</html>

Latest revision as of 23:15, 1 November 2017

HOME
TEAM
PROJECT
Project
Description
Design
Experiments
Notebook
InterLab
Results
Demonstrate
Attributions
DELIVERABLES
PARTS
Parts
Basic Parts
Part Collection
SAFETY
HUMAN PRACTICES
Integrated and Silver/Gold
Public Engagement
AWARDS
Entrepreneurship
Applied Design
Hardware
JUDGING FORM

USMA-West_Point


Design - iGem 01

usMA-WEST POINT


Design



The team has provided two sequences to produce odorant receptors in animal neurons that have been read on two different devices using fluorescence or field potential measurements and can be utilized with static or dynamic devices. This vision allows us to not only use receptors from the human genome but use receptors across the complete animal kingdom producing a device that expands upon any species’ current method of odorant detection. This vision allows us to identify all smells that have been selected throughout the entire animal kingdom and does not confusion perception with sensation. Better understanding this technology will allow the team to model specific odorant receptors that are presently unknown to detect odorants that currently do not have odorant receptors and are therefore “undetectable”.



Plasmid Design



Plasmid design and development is a key component to the eNose Project. Here, we look at the gPCR found in the olfactory system responsible for detecting the sense of smell. Specifically, olfactory neurons contain receptor proteins known as G protein-coupled receptors that recognize when a specific odorant ligand is present causing the generation of an action potential from the cell that is easily measurable with current cell physiology tools and photonic systems.



Device Design



The team has built two devices using two different sensing mechanisms: 1) a measured intensity change caused by fluorescence due to calcium binding, and 2) a measured voltage change generated by an action potential. An Arduino was programed and developed to measure low level fluorescent intensity from calcium binding compounds. The Arduino system can be read through Bluetooth or connected to a computer making it versatile for multiple applications. Connecting via Bluetooth allows the system to also collect time and location data to enhance detection capabilities. LED also offer an initial warning to alert the user of any potential threats. The second device is a peristaltic flow chamber with inputs and outputs to transport new media and odorant samples. The cells rest on a microelectrode array that measures field potential measurements during the voltage change of a cell action potential, which is a more sensitive and electronic measurement.



Hand Held Photosensor Design



A hand held photosensor device was designed to measure the change in fluorescence caused by cell depolarization in the presence of calcium binding ion. A the device is small making it portable, light weight, and inexpensive that can be used with other computational systems to analyze odorant mixtures.

​

An Arduino system was utilized as the initial system to measure the voltage change during increased fluorescence caused by depolarization. The Arduino was Bluetooth compatible allowing voltage measurements to transfer to a portable mobile device while also providing location and time. Additionally, the data could be hard wired to a computer providing the change in voltage.

​

Coding was used to determine fluorescent intensity and relay the information through a color display and through colored lights allowing the user to know when they were in the presence of a scent.



Microelectrode Array Bioreactor



A bioreactor was designed to create a closed system that would hold the MEA and be able to capture the neurons’ output signal the neurons. Autodesk Inventor was used to render two pieces with ports and internal channels and printed using a FormLabs Form 2 3D printer.

​

The MEA pad consists of electrodes to which the cells adhere, leads that transport the signal, contact pads that transfer the signal to a circuit board, and a layer of insulation. Due to complications in manufacturing a custom design, we resorted to purchasing prefabricated MEAs. Two different MEA designs were purchased, standard and perforated.

​

Since the bioreactor created a closed system, a perfusion system was developed to allow continual media flow keeping the cells alive without exposing them to the atmosphere, avoiding potential bacteria contamination. This was done by using a peristaltic pump which regulated the flow of media through the system. Olfactants were injected onto the cells through a syringe in the vented screw port at the top of the bioreactor. As olfactant was introduced, pressure was kept constant by withdrawing media and air from another syringe on the other vented screw port on the top of the bioreactor.



Signal Processing



The search for alternative methods to detect libraries of volatile chemicals is an ongoing endeavor, and for the past 20 years improvements to biosensing has enhanced in speed, sensitivity, and cost. However, these methods are still chemically blind resulting in anosmia of several odors that surround us.


Signal processing will be the method to analyze the action potential of the electrical signal and remove perception from sensation. Measuring the intensity of an action potential can create electrical fingerprints based on voltage intensity and specific olfactory receptor firing in the presence of an analyte. Therefore, removing the animal and leaving the necessary cells can improve detection specificity by measuring action potentials from olfactory receptor binding of a ligand to individual neurons to produce living biosensors that are selectively persistent in a local environment and can detect analytes with novel capabilities.



DEPARTMENT OF CHEMISTRY AND LIFE SCIENCE



BARTLETT HALL, ROOM 400, WEST POINT, NY 10996, USA


TEL: 845.938.3915