Difference between revisions of "Team:USMA-West Point/Experiments"

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HT22 Media Preparation
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<ol>
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<li>Warm DMEM up (about 20 minutes in a 37 &#8451 water bath)
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<li>Add Fetal Bovine Serum (FBS)
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<li>Add Penicillin Streptomycin (Penstrep)
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<li>Final concentrations should be 10% FBS and 1% Penstrep
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</ol>
  
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<t>Plating Cells
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<ol>
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<li>Remove cells from liquid nitrogen
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<li>Warm up cells in a water bath until a small crystal remains
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<li>Pipette out media (1 mL) and place in a 10 mL Falcon tube
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<li>Add 5 mL of media to the tube
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<li>Centrifuge at 150 rcf for 5 min
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<li>Vacuum out media without disturbing pellet
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<li>Break pellet with 12 mL of media
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<li>Distribute 2 mL into each well of a 6 well plate
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<li>Incubate cells at 37 &#8451 and 5% CO2
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</ol>
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</p>
 
</html>
 
</html>

Revision as of 12:43, 31 July 2017

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USMA-West_Point

Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

What should this page contain?
  • Protocols
  • Experiments
  • Documentation of the development of your project
Inspiration


HT22 Media Preparation

  1. Warm DMEM up (about 20 minutes in a 37 &#8451 water bath)
  2. Add Fetal Bovine Serum (FBS)
  3. Add Penicillin Streptomycin (Penstrep)
  4. Final concentrations should be 10% FBS and 1% Penstrep
Plating Cells
  1. Remove cells from liquid nitrogen
  2. Warm up cells in a water bath until a small crystal remains
  3. Pipette out media (1 mL) and place in a 10 mL Falcon tube
  4. Add 5 mL of media to the tube
  5. Centrifuge at 150 rcf for 5 min
  6. Vacuum out media without disturbing pellet
  7. Break pellet with 12 mL of media
  8. Distribute 2 mL into each well of a 6 well plate
  9. Incubate cells at 37 &#8451 and 5% CO2