Difference between revisions of "Team:USMA-West Point/InterLab"

Line 1: Line 1:
 
{{USMA-West_Point}}
 
{{USMA-West_Point}}
  
−
<!DOCTYPE html>
+
 
 
<html xmlns="http://www.w3.org/1999/xhtml">
 
<html xmlns="http://www.w3.org/1999/xhtml">
 
<head><meta name='robots' content='noindex, nofollow'>
 
<head><meta name='robots' content='noindex, nofollow'>
Line 380: Line 380:
 
<ul class="submenu">
 
<ul class="submenu">
 
 
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/basic-parts2" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="90d18fdb" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Parts</span>
 +
</a>
 
 
 
</li>
 
</li>
Line 672: Line 686:
 
 
 
<div id="element-f9330594bb729d2" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 
<div id="element-f9330594bb729d2" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
−
<p>Reliable and repeatable measurement is a key component to all engineering disciplines. The same holds true for synthetic biology, which has also been called <b>engineering</b> biology. However, the ability to repeat measurements in different labs has been difficult. The Measurement Committee, through the InterLab study, has been developing a robust measurement procedure for green fluorescent protein (GFP) over the last three years. We chose GFP as the measurement marker for this study since it's one of the most used markers in synthetic biology and, as a result, most laboratories are equipped to measure this protein.</p>
+
<p><span style="background-color:#000000;">Reliable and repeatable measurement is a key component to all engineering disciplines. The same holds true for synthetic biology, which has also been called </span><b><span style="background-color:#000000;">engineering</span></b><span style="background-color:#000000;"> biology. However, the ability to repeat measurements in different labs has been difficult. The Measurement Committee, through the InterLab study, has been developing a robust measurement procedure for green fluorescent protein (GFP) over the last three years. We chose GFP as the measurement marker for this study since it's one of the most used markers in synthetic biology and, as a result, most laboratories are equipped to measure this protein.</span><br></p>
 
</div>
 
</div>
 
 

Revision as of 11:12, 1 November 2017

HOME
TEAM
PROJECT
Project
Description
Design
Experiments
Notebook
InterLab
Results
Demonstrate
Attributions
DELIVERABLES
PARTS
Parts
Basic Parts
Part Collection
SAFETY
HUMAN PRACTICES
Integrated and Silver/Gold
Public Engagement
AWARDS
Entrepreneurship
Applied Design
Hardware
JUDGING FORM

USMA-West_Point


Experiments - iGem 01

usMA-WEST POINT


Interlab Study



Reliable and repeatable measurement is a key component to all engineering disciplines. The same holds true for synthetic biology, which has also been called engineering biology. However, the ability to repeat measurements in different labs has been difficult. The Measurement Committee, through the InterLab study, has been developing a robust measurement procedure for green fluorescent protein (GFP) over the last three years. We chose GFP as the measurement marker for this study since it's one of the most used markers in synthetic biology and, as a result, most laboratories are equipped to measure this protein.



Fluorescein Standard Curve



Standard Curves



Raw Absorbance 600 Readings



(Log Scale) Fluorescein Standard Curve



Fluorescein Standard Curve



Raw Fluorescence Readings



DEPARTMENT OF CHEMISTRY AND LIFE SCIENCE



BARTLETT HALL, ROOM 400, WEST POINT, NY 10996, USA


TEL: 845.938.3915