Difference between revisions of "Team:USMA-West Point/InterLab"

 
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{{USMA-West_Point}}
 
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<html xmlns="http://www.w3.org/1999/xhtml">
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<head><meta name='robots' content='noindex, nofollow'>
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<title>
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Experiments - iGem 01</title>
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<meta charset="UTF-8">
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<meta name="viewport" content="width=device-width, maximum-scale=1"/>
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<div class="column full_size">
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<h1>InterLab</h1>
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</br>
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<link rel="icon" type="image/png" href="https://lh3.googleusercontent.com/dd_e0xA19up9208Tv6odcjHEw6z4cKAA6fTgjZ9ynkKoSnr5R4vFxI7gZp6pnZH1Vi5T5f-fphjFsrLG=s30" />
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<h2>InterLab Results</h2>
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<h3>Raw Plate Readings</h3>
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<h4>Fluorescence Raw Readings:</h4>
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<p>
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<table class="tableizer-table">
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<thead><tr class="tableizer-firstrow"><th>Hour 0:</th><th>Neg. Control</th><th>Pos. Control</th><th>Device 1</th><th>Device 2</th><th>Device 3</th><th>Device 4</th><th>Device 5</th><th>Device 6</th><th>LB + Chlor (blank)</th></tr></thead><tbody>
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<script src="https://code.jquery.com/jquery-2.x-git.min.js" type="text/javascript"></script>
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  <tr><td>Colony 1, Replicate 1</td><td>4518</td><td>4993</td><td>4653</td><td>4766</td><td>4542</td><td>4881</td><td>4363</td><td>4337</td><td>3917</td></tr>
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<script src="https://maps.googleapis.com/maps/api/js?key=AIzaSyByWRZWFbQRxJM7SiNz2NURPDPJPqTtBQQ" type="text/javascript"></script>
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<tr><td>Colony 1, Replicate 2</td><td>3170</td><td>3942</td><td>3840</td><td>3578</td><td>3573</td><td>3981</td><td>3528</td><td>3328</td><td>3018</td></tr>
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<tr><td>Colony 1, Replicate 3</td><td>3503</td><td>4352</td><td>3832</td><td>3499</td><td>3491</td><td>4099</td><td>3518</td><td>3071</td><td>3011</td></tr>
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<tr><td>Colony 1, Replicate 4</td><td>3394</td><td>4358</td><td>3625</td><td>3561</td><td>3531</td><td>3643</td><td>3224</td><td>3002</td><td>3250</td></tr>
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<tr><td>Colony 2, Replicate 1</td><td>3245</td><td>4674</td><td>3538</td><td>3614</td><td>3384</td><td>3005</td><td>2798</td><td>3002</td><td>3090</td></tr>
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<script src="https://imos004-dot-im--os.appspot.com/js/imos.js?v=1.4.1f" type="text/javascript"></script>
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<tr><td>Colony 2, Replicate 2</td><td>3506</td><td>4339</td><td>3414</td><td>3447</td><td>3402</td><td>3669</td><td>3398</td><td>3285</td><td>3087</td></tr>
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<tr><td>Colony 2, Replicate 3</td><td>3539</td><td>4159</td><td>3728</td><td>3503</td><td>3273</td><td>3532</td><td>3639</td><td>3167</td><td>3136</td></tr>
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<tr><td>Colony 2, Replicate 4</td><td>4163</td><td>5110</td><td>4287</td><td>4337</td><td>4215</td><td>4572</td><td>4562</td><td>3933</td><td>3824</td></tr>
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<thead><tr class="tableizer-firstrow"><th>Hour 2:</th><th>Neg. Control</th><th>Pos. Control</th><th>Device 1</th><th>Device 2</th><th>Device 3</th><th>Device 4</th><th>Device 5</th><th>Device 6</th><th>LB + Chlor (blank)</th></tr></thead><tbody>
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<tr><td>Colony 1, Replicate 1</td><td>4668</td><td>10019</td><td>5437</td><td>4451</td><td>4503</td><td>5924</td><td>6914</td><td>4276</td><td>3874</td></tr>
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<tr><td>Colony 1, Replicate 2</td><td>3600</td><td>8032</td><td>4214</td><td>3758</td><td>3855</td><td>6042</td><td>5391</td><td>3829</td><td>3461</td></tr>
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<tr><td>Colony 1, Replicate 3</td><td>3851</td><td>8095</td><td>4501</td><td>4209</td><td>3875</td><td>5661</td><td>5692</td><td>4013</td><td>3605</td></tr>
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<tr><td>Colony 1, Replicate 4</td><td>3708</td><td>8608</td><td>4023</td><td>3760</td><td>3905</td><td>5399</td><td>5151</td><td>3955</td><td>3943</td></tr>
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  <tr><td>Colony 2, Replicate 1</td><td>3832</td><td>10245</td><td>4223</td><td>3958</td><td>3761</td><td>5076</td><td>4693</td><td>3674</td><td>3576</td></tr>
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<tr><td>Colony 2, Replicate 2</td><td>4284</td><td>9802</td><td>3912</td><td>3795</td><td>4241</td><td>5050</td><td>5865</td><td>3998</td><td>3653</td></tr>
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<tr><td>Colony 2, Replicate 3</td><td>3592</td><td>9224</td><td>4333</td><td>3743</td><td>4305</td><td>5932</td><td>5409</td><td>3829</td><td>3436</td></tr>
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<tr><td>Colony 2, Replicate 4</td><td>4489</td><td>10702</td><td>4283</td><td>4350</td><td>4674</td><td>6221</td><td>6458</td><td>3965</td><td>3732</td></tr>
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<thead><tr class="tableizer-firstrow"><th>Hour 4:</th><th>Neg. Control</th><th>Pos. Control</th><th>Device 1</th><th>Device 2</th><th>Device 3</th><th>Device 4</th><th>Device 5</th><th>Device 6</th><th>LB + Chlor (blank)</th></tr></thead><tbody>
 
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<tr><td>Colony 1, Replicate 1</td><td>4555</td><td>22301</td><td>5946</td><td>4486</td><td>5307</td><td>13006</td><td>9913</td><td>4981</td><td>3676</td></tr>
 
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<tr><td>Colony 1, Replicate 2</td><td>3884</td><td>17341</td><td>4727</td><td>3766</td><td>4061</td><td>10566</td><td>7998</td><td>3620</td><td>3053</td></tr>
 
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<tr><td>Colony 1, Replicate 3</td><td>4459</td><td>17059</td><td>4574</td><td>3762</td><td>4424</td><td>10813</td><td>7914</td><td>3953</td><td>3270</td></tr>
 
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<tr><td>Colony 1, Replicate 4</td><td>3749</td><td>15078</td><td>4809</td><td>4062</td><td>3801</td><td>9746</td><td>8122</td><td>4082</td><td>3477</td></tr>
 
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<tr><td>Colony 2, Replicate 1</td><td>3968</td><td>21606</td><td>3888</td><td>3106</td><td>4626</td><td>8642</td><td>7060</td><td>3679</td><td>2999</td></tr>
 
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<tr><td>Colony 2, Replicate 2</td><td>4252</td><td>22865</td><td>3669</td><td>3937</td><td>4864</td><td>8556</td><td>7699</td><td>3843</td><td>3157</td></tr>
 
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<tr><td>Colony 2, Replicate 3</td><td>4082</td><td>21345</td><td>3680</td><td>3832</td><td>4616</td><td>9756</td><td>8910</td><td>3665</td><td>3379</td></tr>
 
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<tr><td>Colony 2, Replicate 4</td><td>4875</td><td>23705</td><td>4335</td><td>4716</td><td>5121</td><td>10526</td><td>8817</td><td>4294</td><td>3424</td></tr>
 
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<!-- Serving analytics -->
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<thead><tr class="tableizer-firstrow"><th>Hour 6:</th><th>Neg. Control</th><th>Pos. Control</th><th>Device 1</th><th>Device 2</th><th>Device 3</th><th>Device 4</th><th>Device 5</th><th>Device 6</th><th>LB + Chlor (blank)</th></tr></thead><tbody>
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<tr><td>Colony 1, Replicate 1</td><td>5607</td><td>39670</td><td>8664</td><td>4790</td><td>6564</td><td>28592</td><td>13193</td><td>5553</td><td>3856</td></tr>
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<tr><td>Colony 1, Replicate 2</td><td>4659</td><td>34413</td><td>7898</td><td>3912</td><td>6683</td><td>27619</td><td>12458</td><td>5442</td><td>3737</td></tr>
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<tr><td>Colony 1, Replicate 3</td><td>4966</td><td>33715</td><td>7763</td><td>4222</td><td>5364</td><td>29942</td><td>11917</td><td>5102</td><td>3620</td></tr>
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<tr><td>Colony 1, Replicate 4</td><td>5134</td><td>35922</td><td>7513</td><td>4479</td><td>5504</td><td>24153</td><td>13827</td><td>4456</td><td>3787</td></tr>
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<tr><td>Colony 2, Replicate 1</td><td>5032</td><td>43982</td><td>4268</td><td>4519</td><td>4988</td><td>14225</td><td>9352</td><td>4567</td><td>3566</td></tr>
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<tr><td>Colony 2, Replicate 2</td><td>6081</td><td>42484</td><td>5198</td><td>4387</td><td>5463</td><td>15398</td><td>12141</td><td>5172</td><td>3776</td></tr>
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<tr><td>Colony 2, Replicate 3</td><td>5219</td><td>46343</td><td>4742</td><td>4460</td><td>5261</td><td>17009</td><td>11819</td><td>5425</td><td>3538</td></tr>
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<tr><td>Colony 2, Replicate 4</td><td>6529</td><td>46660</td><td>4472</td><td>4727</td><td>5416</td><td>16835</td><td>13157</td><td>5423</td><td>4467</td></tr>
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</tbody></table>
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</p>
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</br>
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<h3>Abs600 Raw Readings:</h3>
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</div>
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<p>
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<div class="helper-div">
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<a href="https://2017.igem.org/Team:USMA-West_Point" data-link-type="EXISTING"  target="_self" >
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<h2 id="vbid-7d38c6a1-8ye3bio7" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >usMA-WEST POINT<br></h2>
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<table class="tableizer-table">
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<thead><tr class="tableizer-firstrow"><th>Hour 0:</th><th>Neg. Control</th><th>Pos. Control</th><th>Device 1</th><th>Device 2</th><th>Device 3</th><th>Device 4</th><th>Device 5</th><th>Device 6</th><th>LB + Chlor (blank)</th></tr></thead><tbody>
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<tr><td>Colony 1, Replicate 1</td><td>0.1</td><td>0.093</td><td>0.093</td><td>0.089</td><td>0.09</td><td>0.092</td><td>0.093</td><td>0.092</td><td>0.087</td></tr>
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−
<tr><td>Colony 1, Replicate 2</td><td>0.092</td><td>0.091</td><td>0.088</td><td>0.09</td><td>0.1</td><td>0.092</td><td>0.093</td><td>0.092</td><td>0.088</td></tr>
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−
<tr><td>Colony 1, Replicate 3</td><td>0.09</td><td>0.091</td><td>0.092</td><td>0.089</td><td>0.093</td><td>0.092</td><td>0.092</td><td>0.091</td><td>0.087</td></tr>
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−
<tr><td>Colony 1, Replicate 4</td><td>0.09</td><td>0.091</td><td>0.088</td><td>0.089</td><td>0.09</td><td>0.094</td><td>0.097</td><td>0.087</td><td>0.091</td></tr>
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<tr><td>Colony 2, Replicate 1</td><td>0.111</td><td>0.096</td><td>0.109</td><td>0.117</td><td>0.093</td><td>0.106</td><td>0.094</td><td>0.092</td><td>0.133</td></tr>
+
−
<tr><td>Colony 2, Replicate 2</td><td>0.091</td><td>0.095</td><td>0.088</td><td>0.09</td><td>0.092</td><td>0.09</td><td>0.092</td><td>0.09</td><td>0.087</td></tr>
+
−
<tr><td>Colony 2, Replicate 3</td><td>0.091</td><td>0.094</td><td>0.089</td><td>0.09</td><td>0.091</td><td>0.092</td><td>0.092</td><td>0.093</td><td>0.087</td></tr>
+
−
<tr><td>Colony 2, Replicate 4</td><td>0.095</td><td>0.094</td><td>0.089</td><td>0.091</td><td>0.094</td><td>0.092</td><td>0.093</td><td>0.091</td><td>0.087</td></tr>
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<thead><tr class="tableizer-firstrow"><th>Hour 2:</th><th>Neg. Control</th><th>Pos. Control</th><th>Device 1</th><th>Device 2</th><th>Device 3</th><th>Device 4</th><th>Device 5</th><th>Device 6</th><th>LB + Chlor (blank)</th></tr></thead><tbody>
+
−
<tr><td>Colony 1, Replicate 1</td><td>0.108</td><td>0.117</td><td>0.108</td><td>0.097</td><td>0.108</td><td>0.093</td><td>0.111</td><td>0.122</td><td>0.086</td></tr>
+
−
<tr><td>Colony 1, Replicate 2</td><td>0.107</td><td>0.107</td><td>0.108</td><td>0.096</td><td>0.107</td><td>0.094</td><td>0.11</td><td>0.124</td><td>0.085</td></tr>
+
−
<tr><td>Colony 1, Replicate 3</td><td>0.107</td><td>0.106</td><td>0.103</td><td>0.095</td><td>0.105</td><td>0.091</td><td>0.107</td><td>0.116</td><td>0.084</td></tr>
+
−
<tr><td>Colony 1, Replicate 4</td><td>0.11</td><td>0.112</td><td>0.102</td><td>0.099</td><td>0.106</td><td>0.092</td><td>0.106</td><td>0.12</td><td>0.087</td></tr>
+
−
<tr><td>Colony 2, Replicate 1</td><td>0.114</td><td>0.115</td><td>0.096</td><td>0.105</td><td>0.134</td><td>0.105</td><td>0.115</td><td>0.126</td><td>0.085</td></tr>
+
−
<tr><td>Colony 2, Replicate 2</td><td>0.12</td><td>0.112</td><td>0.098</td><td>0.107</td><td>0.131</td><td>0.104</td><td>0.111</td><td>0.116</td><td>0.085</td></tr>
+
−
<tr><td>Colony 2, Replicate 3</td><td>0.118</td><td>0.113</td><td>0.1</td><td>0.103</td><td>0.119</td><td>0.105</td><td>0.107</td><td>0.119</td><td>0.088</td></tr>
+
−
<tr><td>Colony 2, Replicate 4</td><td>0.119</td><td>0.111</td><td>0.098</td><td>0.105</td><td>0.126</td><td>0.107</td><td>0.111</td><td>0.119</td><td>0.087</td></tr>
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<thead><tr class="tableizer-firstrow"><th>Hour 4:</th><th>Neg. Control</th><th>Pos. Control</th><th>Device 1</th><th>Device 2</th><th>Device 3</th><th>Device 4</th><th>Device 5</th><th>Device 6</th><th>LB + Chlor (blank)</th></tr></thead><tbody>
+
−
<tr><td>Colony 1, Replicate 1</td><td>0.207</td><td>0.232</td><td>0.328</td><td>0.277</td><td>0.236</td><td>0.124</td><td>0.218</td><td>0.265</td><td>0.084</td></tr>
+
−
<tr><td>Colony 1, Replicate 2</td><td>0.21</td><td>0.205</td><td>0.304</td><td>0.268</td><td>0.214</td><td>0.121</td><td>0.21</td><td>0.24</td><td>0.083</td></tr>
+
−
<tr><td>Colony 1, Replicate 3</td><td>0.215</td><td>0.198</td><td>0.303</td><td>0.272</td><td>0.21</td><td>0.117</td><td>0.201</td><td>0.247</td><td>0.085</td></tr>
+
−
<tr><td>Colony 1, Replicate 4</td><td>0.202</td><td>0.194</td><td>0.317</td><td>0.272</td><td>0.206</td><td>0.115</td><td>0.208</td><td>0.264</td><td>0.085</td></tr>
+
−
<tr><td>Colony 2, Replicate 1</td><td>0.227</td><td>0.217</td><td>0.255</td><td>0.293</td><td>0.259</td><td>0.181</td><td>0.221</td><td>0.24</td><td>0.084</td></tr>
+
−
<tr><td>Colony 2, Replicate 2</td><td>0.227</td><td>0.214</td><td>0.238</td><td>0.301</td><td>0.254</td><td>0.18</td><td>0.203</td><td>0.235</td><td>0.084</td></tr>
+
−
<tr><td>Colony 2, Replicate 3</td><td>0.226</td><td>0.205</td><td>0.231</td><td>0.285</td><td>0.243</td><td>0.184</td><td>0.205</td><td>0.234</td><td>0.088</td></tr>
+
−
<tr><td>Colony 2, Replicate 4</td><td>0.219</td><td>0.196</td><td>0.232</td><td>0.301</td><td>0.235</td><td>0.18</td><td>0.208</td><td>0.236</td><td>0.087</td></tr>
+
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table.tableizer-table {
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<table class="tableizer-table">
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<thead><tr class="tableizer-firstrow"><th>Hour 6:</th><th>Neg. Control</th><th>Pos. Control</th><th>Device 1</th><th>Device 2</th><th>Device 3</th><th>Device 4</th><th>Device 5</th><th>Device 6</th><th>LB + Chlor (blank)</th></tr></thead><tbody>
+
−
<tr><td>Colony 1, Replicate 1</td><td>0.369</td><td>0.388</td><td>0.564</td><td>0.544</td><td>0.402</td><td>0.218</td><td>0.394</td><td>0.413</td><td>0.087</td></tr>
+
−
<tr><td>Colony 1, Replicate 2</td><td>0.378</td><td>0.395</td><td>0.579</td><td>0.52</td><td>0.435</td><td>0.235</td><td>0.4</td><td>0.441</td><td>0.089</td></tr>
+
−
<tr><td>Colony 1, Replicate 3</td><td>0.368</td><td>0.399</td><td>0.576</td><td>0.549</td><td>0.407</td><td>0.239</td><td>0.369</td><td>0.452</td><td>0.086</td></tr>
+
−
<tr><td>Colony 1, Replicate 4</td><td>0.332</td><td>0.377</td><td>0.562</td><td>0.492</td><td>0.408</td><td>0.235</td><td>0.411</td><td>0.406</td><td>0.09</td></tr>
+
−
<tr><td>Colony 2, Replicate 1</td><td>0.411</td><td>0.437</td><td>0.51</td><td>0.564</td><td>0.443</td><td>0.376</td><td>0.42</td><td>0.445</td><td>0.087</td></tr>
+
−
<tr><td>Colony 2, Replicate 2</td><td>0.426</td><td>0.413</td><td>0.539</td><td>0.55</td><td>0.442</td><td>0.377</td><td>0.425</td><td>0.454</td><td>0.087</td></tr>
+
−
<tr><td>Colony 2, Replicate 3</td><td>0.419</td><td>0.456</td><td>0.528</td><td>0.565</td><td>0.443</td><td>0.374</td><td>0.436</td><td>0.459</td><td>0.088</td></tr>
+
−
<tr><td>Colony 2, Replicate 4</td><td>0.429</td><td>0.436</td><td>0.538</td><td>0.575</td><td>0.422</td><td>0.376</td><td>0.409</td><td>0.471</td><td>0.089</td></tr>
+
−
</tbody></table>
+
−
</p>
+
  
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<p><span style="background-color:#000000;">Reliable and repeatable measurement is a key component to all engineering disciplines. The same holds true for synthetic biology, which has also been called </span><b><span style="background-color:#000000;">engineering</span></b><span style="background-color:#000000;"> biology. However, the ability to repeat measurements in different labs has been difficult. The Measurement Committee, through the InterLab study, has been developing a robust measurement procedure for green fluorescent protein (GFP) over the last three years. We chose GFP as the measurement marker for this study since it's one of the most used markers in synthetic biology and, as a result, most laboratories are equipped to measure this protein.</span><br></p>
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USMA-West_Point


Experiments - iGem 01

usMA-WEST POINT


Interlab Study



Reliable and repeatable measurement is a key component to all engineering disciplines. The same holds true for synthetic biology, which has also been called engineering biology. However, the ability to repeat measurements in different labs has been difficult. The Measurement Committee, through the InterLab study, has been developing a robust measurement procedure for green fluorescent protein (GFP) over the last three years. We chose GFP as the measurement marker for this study since it's one of the most used markers in synthetic biology and, as a result, most laboratories are equipped to measure this protein.



Fluorescein Standard Curve



Standard Curves



Raw Absorbance 600 Readings



(Log Scale) Fluorescein Standard Curve



Fluorescein Standard Curve



Raw Fluorescence Readings



DEPARTMENT OF CHEMISTRY AND LIFE SCIENCE



BARTLETT HALL, ROOM 400, WEST POINT, NY 10996, USA


TEL: 845.938.3915