USMA-West_Point
InterLab
InterLab Results
Fluorescein Standard Curve Data
uM Fluorescein | 50.00 | 25 | 12.5 | 6.25 | 3.125 | 1.5625 | 0.78125 | 0.390625 | 0.1953125 | 0.09765625 | 0.048828125 | 0 |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Replicate 1 | 47320 | 33855 | 23725 | 14221 | 7934 | 3698 | 2048 | 1073 | 509 | 235 | 127 | 5 |
Replicate 2 | 42907 | 30572 | 18789 | 9977 | 5305 | 2626 | 1366 | 654 | 359 | 192 | 86 | 6 |
Replicate 3 | 42495 | 30452 | 18095 | 10220 | 5688 | 2955 | 1408 | 683 | 363 | 174 | 85 | 7 |
Replicate 4 | 43951 | 31123 | 19060 | 11395 | 5879 | 2878 | 1515 | 758 | 371 | 192 | 87 | 6 |
Arith. Mean | 44168.25 | 31500.5 | 19917.25 | 11453.25 | 6201.5 | 3039.25 | 1584.25 | 792 | 400.5 | 198.25 | 96.25 | 6 |
Arith. Std.Dev. | 2188.701499 | 1596.625713 | 2570.822226 | 1946.283017 | 1179.400554 | 461.0953444 | 315.4661894 | 192.3902284 | 72.50517223 | 25.92778432 | 20.51625372 | 0.816496581 |
Raw Plate Readings
Fluorescence Raw Readings:
Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 4518 | 4993 | 4653 | 4766 | 4542 | 4881 | 4363 | 4337 | 3917 |
Colony 1, Replicate 2 | 3170 | 3942 | 3840 | 3578 | 3573 | 3981 | 3528 | 3328 | 3018 |
Colony 1, Replicate 3 | 3503 | 4352 | 3832 | 3499 | 3491 | 4099 | 3518 | 3071 | 3011 |
Colony 1, Replicate 4 | 3394 | 4358 | 3625 | 3561 | 3531 | 3643 | 3224 | 3002 | 3250 |
Colony 2, Replicate 1 | 3245 | 4674 | 3538 | 3614 | 3384 | 3005 | 2798 | 3002 | 3090 |
Colony 2, Replicate 2 | 3506 | 4339 | 3414 | 3447 | 3402 | 3669 | 3398 | 3285 | 3087 |
Colony 2, Replicate 3 | 3539 | 4159 | 3728 | 3503 | 3273 | 3532 | 3639 | 3167 | 3136 |
Colony 2, Replicate 4 | 4163 | 5110 | 4287 | 4337 | 4215 | 4572 | 4562 | 3933 | 3824 |
Hour 2: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 4668 | 10019 | 5437 | 4451 | 4503 | 5924 | 6914 | 4276 | 3874 |
Colony 1, Replicate 2 | 3600 | 8032 | 4214 | 3758 | 3855 | 6042 | 5391 | 3829 | 3461 |
Colony 1, Replicate 3 | 3851 | 8095 | 4501 | 4209 | 3875 | 5661 | 5692 | 4013 | 3605 |
Colony 1, Replicate 4 | 3708 | 8608 | 4023 | 3760 | 3905 | 5399 | 5151 | 3955 | 3943 |
Colony 2, Replicate 1 | 3832 | 10245 | 4223 | 3958 | 3761 | 5076 | 4693 | 3674 | 3576 |
Colony 2, Replicate 2 | 4284 | 9802 | 3912 | 3795 | 4241 | 5050 | 5865 | 3998 | 3653 |
Colony 2, Replicate 3 | 3592 | 9224 | 4333 | 3743 | 4305 | 5932 | 5409 | 3829 | 3436 |
Colony 2, Replicate 4 | 4489 | 10702 | 4283 | 4350 | 4674 | 6221 | 6458 | 3965 | 3732 |
Hour 4: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 4555 | 22301 | 5946 | 4486 | 5307 | 13006 | 9913 | 4981 | 3676 |
Colony 1, Replicate 2 | 3884 | 17341 | 4727 | 3766 | 4061 | 10566 | 7998 | 3620 | 3053 |
Colony 1, Replicate 3 | 4459 | 17059 | 4574 | 3762 | 4424 | 10813 | 7914 | 3953 | 3270 |
Colony 1, Replicate 4 | 3749 | 15078 | 4809 | 4062 | 3801 | 9746 | 8122 | 4082 | 3477 |
Colony 2, Replicate 1 | 3968 | 21606 | 3888 | 3106 | 4626 | 8642 | 7060 | 3679 | 2999 |
Colony 2, Replicate 2 | 4252 | 22865 | 3669 | 3937 | 4864 | 8556 | 7699 | 3843 | 3157 |
Colony 2, Replicate 3 | 4082 | 21345 | 3680 | 3832 | 4616 | 9756 | 8910 | 3665 | 3379 |
Colony 2, Replicate 4 | 4875 | 23705 | 4335 | 4716 | 5121 | 10526 | 8817 | 4294 | 3424 |
Hour 6: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 5607 | 39670 | 8664 | 4790 | 6564 | 28592 | 13193 | 5553 | 3856 |
Colony 1, Replicate 2 | 4659 | 34413 | 7898 | 3912 | 6683 | 27619 | 12458 | 5442 | 3737 |
Colony 1, Replicate 3 | 4966 | 33715 | 7763 | 4222 | 5364 | 29942 | 11917 | 5102 | 3620 |
Colony 1, Replicate 4 | 5134 | 35922 | 7513 | 4479 | 5504 | 24153 | 13827 | 4456 | 3787 |
Colony 2, Replicate 1 | 5032 | 43982 | 4268 | 4519 | 4988 | 14225 | 9352 | 4567 | 3566 |
Colony 2, Replicate 2 | 6081 | 42484 | 5198 | 4387 | 5463 | 15398 | 12141 | 5172 | 3776 |
Colony 2, Replicate 3 | 5219 | 46343 | 4742 | 4460 | 5261 | 17009 | 11819 | 5425 | 3538 |
Colony 2, Replicate 4 | 6529 | 46660 | 4472 | 4727 | 5416 | 16835 | 13157 | 5423 | 4467 |
Abs600 Raw Readings:
Hour 0: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 0.1 | 0.093 | 0.093 | 0.089 | 0.09 | 0.092 | 0.093 | 0.092 | 0.087 |
Colony 1, Replicate 2 | 0.092 | 0.091 | 0.088 | 0.09 | 0.1 | 0.092 | 0.093 | 0.092 | 0.088 |
Colony 1, Replicate 3 | 0.09 | 0.091 | 0.092 | 0.089 | 0.093 | 0.092 | 0.092 | 0.091 | 0.087 |
Colony 1, Replicate 4 | 0.09 | 0.091 | 0.088 | 0.089 | 0.09 | 0.094 | 0.097 | 0.087 | 0.091 |
Colony 2, Replicate 1 | 0.111 | 0.096 | 0.109 | 0.117 | 0.093 | 0.106 | 0.094 | 0.092 | 0.133 |
Colony 2, Replicate 2 | 0.091 | 0.095 | 0.088 | 0.09 | 0.092 | 0.09 | 0.092 | 0.09 | 0.087 |
Colony 2, Replicate 3 | 0.091 | 0.094 | 0.089 | 0.09 | 0.091 | 0.092 | 0.092 | 0.093 | 0.087 |
Colony 2, Replicate 4 | 0.095 | 0.094 | 0.089 | 0.091 | 0.094 | 0.092 | 0.093 | 0.091 | 0.087 |
Hour 2: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 0.108 | 0.117 | 0.108 | 0.097 | 0.108 | 0.093 | 0.111 | 0.122 | 0.086 |
Colony 1, Replicate 2 | 0.107 | 0.107 | 0.108 | 0.096 | 0.107 | 0.094 | 0.11 | 0.124 | 0.085 |
Colony 1, Replicate 3 | 0.107 | 0.106 | 0.103 | 0.095 | 0.105 | 0.091 | 0.107 | 0.116 | 0.084 |
Colony 1, Replicate 4 | 0.11 | 0.112 | 0.102 | 0.099 | 0.106 | 0.092 | 0.106 | 0.12 | 0.087 |
Colony 2, Replicate 1 | 0.114 | 0.115 | 0.096 | 0.105 | 0.134 | 0.105 | 0.115 | 0.126 | 0.085 |
Colony 2, Replicate 2 | 0.12 | 0.112 | 0.098 | 0.107 | 0.131 | 0.104 | 0.111 | 0.116 | 0.085 |
Colony 2, Replicate 3 | 0.118 | 0.113 | 0.1 | 0.103 | 0.119 | 0.105 | 0.107 | 0.119 | 0.088 |
Colony 2, Replicate 4 | 0.119 | 0.111 | 0.098 | 0.105 | 0.126 | 0.107 | 0.111 | 0.119 | 0.087 |
Hour 4: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 0.207 | 0.232 | 0.328 | 0.277 | 0.236 | 0.124 | 0.218 | 0.265 | 0.084 |
Colony 1, Replicate 2 | 0.21 | 0.205 | 0.304 | 0.268 | 0.214 | 0.121 | 0.21 | 0.24 | 0.083 |
Colony 1, Replicate 3 | 0.215 | 0.198 | 0.303 | 0.272 | 0.21 | 0.117 | 0.201 | 0.247 | 0.085 |
Colony 1, Replicate 4 | 0.202 | 0.194 | 0.317 | 0.272 | 0.206 | 0.115 | 0.208 | 0.264 | 0.085 |
Colony 2, Replicate 1 | 0.227 | 0.217 | 0.255 | 0.293 | 0.259 | 0.181 | 0.221 | 0.24 | 0.084 |
Colony 2, Replicate 2 | 0.227 | 0.214 | 0.238 | 0.301 | 0.254 | 0.18 | 0.203 | 0.235 | 0.084 |
Colony 2, Replicate 3 | 0.226 | 0.205 | 0.231 | 0.285 | 0.243 | 0.184 | 0.205 | 0.234 | 0.088 |
Colony 2, Replicate 4 | 0.219 | 0.196 | 0.232 | 0.301 | 0.235 | 0.18 | 0.208 | 0.236 | 0.087 |
Hour 6: | Neg. Control | Pos. Control | Device 1 | Device 2 | Device 3 | Device 4 | Device 5 | Device 6 | LB + Chlor (blank) |
---|---|---|---|---|---|---|---|---|---|
Colony 1, Replicate 1 | 0.369 | 0.388 | 0.564 | 0.544 | 0.402 | 0.218 | 0.394 | 0.413 | 0.087 |
Colony 1, Replicate 2 | 0.378 | 0.395 | 0.579 | 0.52 | 0.435 | 0.235 | 0.4 | 0.441 | 0.089 |
Colony 1, Replicate 3 | 0.368 | 0.399 | 0.576 | 0.549 | 0.407 | 0.239 | 0.369 | 0.452 | 0.086 |
Colony 1, Replicate 4 | 0.332 | 0.377 | 0.562 | 0.492 | 0.408 | 0.235 | 0.411 | 0.406 | 0.09 |
Colony 2, Replicate 1 | 0.411 | 0.437 | 0.51 | 0.564 | 0.443 | 0.376 | 0.42 | 0.445 | 0.087 |
Colony 2, Replicate 2 | 0.426 | 0.413 | 0.539 | 0.55 | 0.442 | 0.377 | 0.425 | 0.454 | 0.087 |
Colony 2, Replicate 3 | 0.419 | 0.456 | 0.528 | 0.565 | 0.443 | 0.374 | 0.436 | 0.459 | 0.088 |
Colony 2, Replicate 4 | 0.429 | 0.436 | 0.538 | 0.575 | 0.422 | 0.376 | 0.409 | 0.471 | 0.089 |