Difference between revisions of "Team:USMA-West Point/Notebook"

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{{USMA-West_Point}}
 
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<!DOCTYPE html>
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<html xmlns="http://www.w3.org/1999/xhtml">
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<head><meta name='robots' content='noindex, nofollow'>
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NOTEBOOK - Demonstrate</title>
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<div class="column full_size">
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<h1>Notebook</h1>
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<script type="text/javascript" src="//www.imcreator.com/all_js.js?v=1.4.1f"></script>
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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<div class="clear"></div>
 
  
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<body class="fast-animated-bg fixed-bg " data-ecommerce-solution="NONE" data-root-id="vbid-10868990-abbmfckb" data-root-style-id="style-10868990-akddbsak" data-default-currency="USD" data-osid="osid--51352934-bd71e811"  data-app-version="1.4.1f" data-caller="static"  data-ecommerce-dashboard="https://dashboard.shoprocket.co" data-static-server="//www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
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<h5>What should this page have?</h5>
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<ul>
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<div id="xprs" data-website-name="Demonstrate" class="xprs-holder" >
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<li>Chronological notes of what your team is doing.</li>
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<div class="main-page "  >
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<li> Brief descriptions of daily important events.</li>
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<div id="content">
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<li>Pictures of your progress. </li>
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<div  id="vbid-c03f00ff-lok1anrm"  class="master container style-c03f00ff-dzebo07f content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="NOTEBOOK"  data-itemslug="notebook" data-itemstyleid="style-c03f00ff-dzebo07f" data-margintop="" data-arranger="stripes" data-layout="multi" data-vbid="vbid-c03f00ff-lok1anrm" data-preset-type-id="ITEMS" data-preview-style="style-c03f00ff-dzebo07f" data-style="style-5a24e-kzdyurm3ju" data-absolute-path="/free/jpruneski8"  >
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<li>Mention who participated in what task.</li>
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<div class="layout-settings" style="display:none;" data-type="multi"></div>
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<div id="children">
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<div class="header-wrapper item-wrapper menus-wrapper ">
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<div class="preview-content-wrapper">
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<div class="preview-content-holder">
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<div class="left-div">
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<div class="logo-holder">
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<div class="item-details menu">
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<h5>Inspiration</h5>
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<p>You can see what others teams have done to organize their notes:</p>
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<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
  
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<ul>  
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</div>
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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</div>
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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</div>
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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<div class="right-div">
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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<div class="benet" style="min-height:inherit;"></div>
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</ul>
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</div>
 
  
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<div class="column full_size">
 
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<h5>Week of 11 September 2017</h5>
 
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<ul>
 
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<li>Attempted transfection of iGEM plasmid, and altered protocol as needed.</li>
 
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<li>Prepared bacteria culture plates for vector amplification.</li>
 
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<li>Purified DNA from cultured bacteria.</li>
 
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</ul>
 
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</br>
 
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<h5>Week of 18 September 2017</h5>
 
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<ul>
 
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<li>Continued transformation of iGEM plasmid.</li>
 
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<li>Prepared chloramphenicol resistant plates.</li>
 
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<li>Cultured psb1c3 cells and made stock.</li>
 
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<li>Created fluorescein calibration curve.</li>
 
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<li>Vectors amplified and purified.</li>
 
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</ul>
 
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</br>
 
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<h5>Week of 25 September 2017</h5>
 
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<ul>
 
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<li>Ran iGEM interlab protocol.</li>
 
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<li>Made and ran gel of plasmids.</li>
 
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<li>Created new glycerol stocks, using resistant bacteria for the process.</li>
 
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</ul>
 
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</br>
 
  
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<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
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</ul>
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<button class="hamburger links-menu-btn  small" type="button">
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</button>
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</div>
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</div>
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</div>
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<!-- LAYOUT SETTINGS -->
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<div class="layout-settings" style="display:none;" data-type="menu"
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data-MENU_OVERLAY="relative"
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data-MENU_SCROLL="false"
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data-ALWAYS_MINIFIED="false"
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data-MENU_POSITION="top"
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data-MENU_ALIGN="left"
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data-BACKGROUND_COLOR="rgb(201, 151, 0)"
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></div>
 
</div>
 
</div>
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</div>
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</div>
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<!-- MENUS END -->
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<!-- TEXT_BLOCK START -->
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<div id="no-image" class="stripe-background load-high-res " ></div>
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<div class="gallery-wrapper item-wrapper text_block-wrapper ">
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<div  class="sub container style-324920b8-6rkb49z5 content flex  custom " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-324920b8-6rkb49z5" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-324920b8-qqcb9mt2" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-324920b8-6rkb49z5" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/jpruneski8"  >
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<!-- ARRANGER SETTINGS -->
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<div class="arranger-settings" style="display:none;"
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data-ARRANGER_TYPE="flex"
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data-ARRANGER_COLS="0"
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data-ARRANGER_ITEM_MAX_WIDTH="400"
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data-ARRANGER_ITEM_MIN_WIDTH="230"
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data-ARRANGER_ITEM_RATIO="1"
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data-ARRANGER_ITEM_SPACING="20"
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data-ARRANGER_ITEMS_PER_PAGE="all"
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data-ARRANGER_ORDER_TYPE="regular"
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data-AUTO_PLAY="true"
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data-AUTO_PLAY_DURATION="4"
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data-FLEX_ELEMENT_EFFECT="effect-fadein"
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></div>
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<div class="layout-settings" style="display:none;" data-type="multi"></div>
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<div id="children">
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<div id="items-holder-wrapper">
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<div id="items-holder">
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<div  id="vbid-324920b8-3icuax6a"  class="sub item-box  page-box style-324920b8-6rkb49z5          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-324920b8-6rkb49z5" data-preview-styleid='style-324920b8-6rkb49z5' data-preset-type-id="UNRESOLVED">
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<div class="page-wrapper item-wrapper ">
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-324920b8-3icuax6a" data-preview-style="style-324920b8-6rkb49z5" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-324920b8-3icuax6a" data-bgimg="">
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<div  class="multi-container preview image-cover" >
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<div class="Picture item-preview">
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<div class="preview-image-holder">
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<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
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</div>
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<div class="helper-div middle-center" >
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<!-- <div class="benet" style="min-height:inherit;"></div> -->
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<div class="pic-side">
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<div class="vertical-aligner">
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<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
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<div class="column full_size">
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<div id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="" data-orig-width="" data-orig-height="" >
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Abbreviations
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<!-- VIDEO TEMPLATE -->
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<ul>
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<li>AM - Angela Marsh</li>
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<li>JG - Dr. Joel Gaston</li>
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<li>KP - Kanak Patel</li>
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<li>MM - Matthew McDonough</li>
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<li>PB - Payton Boylston</li>
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<li>RG - Rayonna Gordon</li>
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<li>WO - Winston Ou</li>
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</ul>
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<a href="#Neurons">Neurons</a> <br>
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<a href="#Perfusion System with Bioreactor Design">Perfusion System with Bioreactor Design</a> <br>
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<a href="#Programming and Electronics">Programming and Electronics (Bioreactor focus)</a> <br>
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<a name="Neurons"></a> <br>
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<h3>Neurons</h3>
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9 Jun 2017 <br> WO RG AM KP MM
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<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
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<ul>
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<li>Received HT-22 Immortalized Mouse Hippocampal Cell Line and related materials from West Point
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</ul>
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13 Jun 2017 <br> WO RG AM KP MM PB
 
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<ul>
 
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<li>Unfroze neurons
 
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<li>Plated neurons for amplification
 
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</ul>
 
  
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15 Jun 2017 <br> WO RG AM KP MM PB
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<li>Change media
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</ul>
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17 Jun 2017 <br> JG
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<!-- RAW TEMPLATE -->
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<ul>
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<li>Changed media
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<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
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</ul>
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19 Jun 2017 <br> WO RG MM PB
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</div>
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<ul>
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<li>Split cells - Passage 1 from receipt of neurons
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</div>
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</ul>
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21 Jun 2017 <br> WO RG MM PB
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</div>
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<ul>  
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</div>
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<li>Changed media
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<div class="text-side shrinker-parent">
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<li>Froze excess cells
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<div class="vertical-aligner">
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</ul>
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<div class="item-details preview-content-wrapper  multi" style="position:relative;">
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<div class="draggable-div-holder"><div id="element-381e48d6d69b748-wrapper" class="draggable-pic-wrapper removable-parent">
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<div id="element-381e48d6d69b748-holder"  class="preview-image-holder draggable-pic-holder removable-parent" data-menu-name="DRAGGABLE_PIC_HOLDER">
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</div><div id="element-34189c69b2334b2-wrapper" class="draggable-pic-wrapper removable-parent">
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</div>
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</div>
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</div></div>
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<div class="preview-content-holder shrinker-content">
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<div class="gallery-wrapper item-wrapper text_block-wrapper ">
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<div  class="sub container style-41e9e943-6rkb49z5 content flex  custom " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-41e9e943-6rkb49z5" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-41e9e943-qqcb9mt2" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-41e9e943-6rkb49z5" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/jpruneski8"  >
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<!-- ARRANGER SETTINGS -->
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<div class="arranger-settings" style="display:none;"
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data-ARRANGER_TYPE="flex"
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data-ARRANGER_ITEM_MIN_WIDTH="230"
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data-ARRANGER_ITEM_RATIO="1"
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data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
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data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
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 +
 +
 +
<div  id="vbid-41e9e943-3icuax6a"  class="sub item-box  page-box style-41e9e943-6rkb49z5          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-41e9e943-6rkb49z5" data-preview-styleid='style-41e9e943-6rkb49z5' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-41e9e943-3icuax6a" data-preview-style="style-41e9e943-6rkb49z5" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-41e9e943-3icuax6a" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
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<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
23 Jun 2017 <br> JG
+
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
−
<ul>  
+
<!-- VIDEO TEMPLATE -->
−
<li>Changed media
+
−
</ul>
+
  
−
27 Jun 2017 <br> WO RG
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
−
<ul>
+
−
<li>Made and changed media
+
−
</ul>
+
  
−
30 Jun 2017 <br> WO RG
 
−
<ul>
 
−
<li>Thawed and plated cells - Passage 2
 
−
</ul>
 
  
−
5 Jul 2017 <br> WO RG
+
<!-- MAP TEMPLATE -->
−
<ul>
+
−
<li>Split cells - Passage 3
+
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
−
</ul>
+
  
−
7 Jul 2017 <br> WO RG
+
<!-- RAW TEMPLATE -->
−
<ul>
+
−
<li>Changed media
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
−
</ul>
+
  
−
10 Jul 2017 <br> WO RG
+
</div>
−
<ul>
+
−
<li>Split cells - Passage 4
+
</div>
−
</ul>
+
  
−
13 Jul 2017 <br> WO RG
+
</div>
−
<ul>
+
</div>
−
<li>Changed media</li>
+
<div class="text-side shrinker-parent">
−
</ul>
+
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
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 +
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 +
</div>
 +
</div>
 +
</div></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
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 +
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 +
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 +
<!-- TEXT_BLOCK END -->
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<!-- TEXT_BLOCK START -->
 +
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 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-d3269007-gm2iekds content flex  custom " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-d3269007-gm2iekds" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-d3269007-nqff64ja" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-d3269007-gm2iekds" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/jpruneski8"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
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data-ARRANGER_ITEM_RATIO="1"
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data-ARRANGER_ITEM_SPACING="20"
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data-ARRANGER_ITEMS_PER_PAGE="all"
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data-ARRANGER_ORDER_TYPE="regular"
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data-AUTO_PLAY_DURATION="4"
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data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
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<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
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<div id="items-holder-wrapper">
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<div id="items-holder">
 +
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<div  id="vbid-d3269007-ovjtodbr"  class="sub item-box  page-box style-d3269007-gm2iekds          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-d3269007-gm2iekds" data-preview-styleid='style-d3269007-gm2iekds' data-preset-type-id="UNRESOLVED">
 +
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<div class="page-wrapper item-wrapper ">
 +
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-d3269007-ovjtodbr" data-preview-style="style-d3269007-gm2iekds" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-d3269007-ovjtodbr" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
17 Jul 2017 <br> JG
+
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
−
<ul>
+
<!-- VIDEO TEMPLATE -->
−
<li>Cells were killed</li>
+
−
</ul>
+
  
−
25 Jul 2017<br> WO RG
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
−
<ul>
+
−
<li>Plated Passage 2 HT22 cells that were previously frozen into a 6 well plate
+
−
</ul>
+
  
−
31 Jul 2017<br> WO JG
 
−
<ul>
 
−
<li>Coated MEA with polylysine
 
−
<li>Seeded 20,000 HT22 cells onto coated MEA
 
−
<li>Incubated said cells
 
−
</ul>
 
  
−
2 August 2017<br> WO RG
+
<!-- MAP TEMPLATE -->
−
<ul>
+
−
<li>Transfected cells on MEA with 3200ng of OR2W1</li>
+
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
−
<li>Incubated transfected cells on MEA in the bioreactor</li>
+
−
</ul> 
+
−
<a name="Perfusion System with Bioreactor Design"></a> <br>
+
−
<h3>Perfusion System with Bioreactor Design</h3>
+
  
−
23 May 2017
+
<!-- RAW TEMPLATE -->
−
<br> WO
+
−
<ul>
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
−
<li>Began bioreactor design based on former models to be used in a perfusion system
+
−
</ul>
+
  
−
25 May 2017
+
</div>
−
<br>WO
+
−
<ul>
+
</div>
−
<li>Used AutoDesk Inventor to design a general bioreactor
+
−
<li>Incorporated shape and general items such as holes for screws and cavity for neuronal culturing
+
−
<li>Determined location of a multielectrode array (MEA) in bioreactor design, where the neurons would be transfected and tested
+
−
</ul>
+
  
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"><div id="vbid-d3269007-0aey4cii-wrapper" class="draggable-pic-wrapper removable-parent">
 +
<div id="vbid-d3269007-0aey4cii-holder"  class="preview-image-holder draggable-pic-holder removable-parent" data-menu-name="DRAGGABLE_PIC_HOLDER">
 +
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 +
</div>
 +
</div>
 +
</div></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
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</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-b3e50235-zvukijf2"  class="master item-box  gallery-box style-b3e50235-yb8q8j7l    button-effects btn_hover3    custom  " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-b3e50235-yb8q8j7l" data-preview-styleid='style-b3e50235-yb8q8j7l' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-b3e50235-yb8q8j7l content flex  custom " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-b3e50235-yb8q8j7l" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-b3e50235-zvukijf2" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-b3e50235-yb8q8j7l" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/jpruneski8"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-b3e50235-ne8j6nq3"  class="sub item-box  page-box style-b3e50235-yb8q8j7l          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-b3e50235-yb8q8j7l" data-preview-styleid='style-b3e50235-yb8q8j7l' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-b3e50235-ne8j6nq3" data-preview-style="style-b3e50235-yb8q8j7l" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-b3e50235-ne8j6nq3" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
30 May 2017 <br> WO
+
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
−
<ul>
+
<!-- VIDEO TEMPLATE -->
−
<li>Adjusted design of bioreactor design
+
−
<li>Began a custom design for a MEA
+
−
</ul>
+
  
−
31 May 2017 <br> WO RG AM KP
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
−
<ul>
+
−
<li>Continued MEA designing: location of electrodes, leads, contact pad spacing, materials
+
−
</ul>
+
  
−
1 Jun 2017 <br> WO RM AM KP
 
−
<ul>
 
−
<li>MEA
 
−
<ul>
 
−
<li>Altered the electrode arrangement and size for a single well of a 96 well plate</li>
 
−
<li>Predetermined specifications: Gold or TiN wires, embedded in glass </li>
 
−
</ul>
 
−
</li>
 
−
</ul>
 
  
−
2 Jun 2017<br>
+
<!-- MAP TEMPLATE -->
−
WO RG AM KP MM
+
−
<ul>
+
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
−
<li>Determined custom MEA design specs, particularly spacing
+
−
</ul>
+
  
−
5 Jun 2017<br>
+
<!-- RAW TEMPLATE -->
−
WO RG AM KP MM
+
−
<ul>
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
−
<li>Revised MEA spacing
+
−
</ul>
+
  
−
6 Jun 2017 <br>
+
</div>
−
WO RG AM KP MM
+
−
<ul>
+
</div>
−
<li>Discussed the location of holes for media flow and a bubble trap
+
−
</ul>
+
  
−
7 Jun 2017 <br>
+
</div>
−
WO RG AM KP MM
+
</div>
−
<ul>
+
<div class="text-side shrinker-parent">
−
<li>Fleshed out design for bubble trap
+
<div class="vertical-aligner">
−
<li>Began design for clamp to keep pins on the multielectrode array (extension of bioreactor) - KP
+
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
−
</ul>
+
<div class="draggable-div-holder"><div id="vbid-b3e50235-3ydgf1h8-wrapper" class="draggable-pic-wrapper removable-parent">
 +
<div id="vbid-b3e50235-3ydgf1h8-holder"  class="preview-image-holder draggable-pic-holder removable-parent" data-menu-name="DRAGGABLE_PIC_HOLDER">
 +
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 +
</div>
 +
</div>
 +
</div></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
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</div>
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</div>
 +
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</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
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<!-- TEXT_BLOCK START -->
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 +
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 +
 +
<div  class="sub container style-41d29184-t1qz2cig content flex  custom " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-41d29184-t1qz2cig" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-41d29184-s8ihtzis" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-41d29184-t1qz2cig" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/jpruneski8"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-41d29184-l9phqyhl"  class="sub item-box  page-box style-41d29184-t1qz2cig          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-41d29184-t1qz2cig" data-preview-styleid='style-41d29184-t1qz2cig' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-41d29184-l9phqyhl" data-preview-style="style-41d29184-t1qz2cig" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-41d29184-l9phqyhl" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
8 Jun 2017 <br> WO RG AM KP MM
+
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
−
<ul>
+
<!-- VIDEO TEMPLATE -->
−
<li>Began discussion on removal of olfactant contaminated media while maintaining a perfusion system via syringes
+
−
<li>Continued clamp design-KP
+
−
<li>Requested a quote for custom MEA
+
−
</ul>
+
  
−
9 Jun 2017 <br> WO RG AM KP MM
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
−
<ul>
+
−
<li>Confirmed the use of a peristaltic pump for media flow
+
−
<li>Finalized MEA design
+
−
</ul>
+
  
−
12 Jun 2017<br>
 
−
WO RG AM KP MM PB
 
−
<ul>
 
−
<li>Contacted other MEA fabrication companies for quotes
 
−
</ul>
 
  
−
13 Jun 2017<br>
+
<!-- MAP TEMPLATE -->
−
WO RG AM KP MM PB
+
−
<ul>
+
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
−
<li>Continued search of MEA vendors
+
−
<li>Worked on specifics regarding flow into and out of the bioreactor/media reservoirs - decided to use luer locks
+
−
</ul>
+
  
−
14 Jun 2017<br>
+
<!-- RAW TEMPLATE -->
−
WO RG AM KP MM PB
+
−
<ul>
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
−
<li>Continued contacting MEA vendors
+
−
</ul>
+
  
−
15 Jun 2017<br>
+
</div>
−
WO RG AM KP MM PB
+
−
<ul>
+
</div>
−
<li>Determined best luer lock connectors for tubing
+
−
<li>Finished printing of clamp design
+
−
<li>Explored photomasking vendors
+
−
</ul>
+
  
−
16 Jun 2017<br>
+
</div>
−
WO RG AM KP MM PB
+
</div>
−
<ul>
+
<div class="text-side shrinker-parent">
−
<li>Determined design of media reservoirs to be conical flasks  
+
<div class="vertical-aligner">
−
<li>Explored pre-made MEA vendors
+
<div class="item-details preview-content-wrapper multi" style="position:relative;">
−
</ul>
+
<div class="draggable-div-holder"><div id="vbid-41d29184-0ipm4six-wrapper" class="draggable-pic-wrapper removable-parent">
 +
<div id="vbid-41d29184-0ipm4six-holder"  class="preview-image-holder draggable-pic-holder removable-parent" data-menu-name="DRAGGABLE_PIC_HOLDER">
 +
<div  id="vbid-41d29184-0ipm4six" class="draggable-pic preview-element  magic-circle-holder load-high-res" data-menu-name="PREVIEW_DRAGGABLE_PIC" style="background-image:url(https://lh3.googleusercontent.com/88ya9aocur-91k-r5SyQYfEahxtPkP6b0k3edKViQH_CQp4BGRDaRnAtYuv3_2-KVKOZSamEUUU-RawQ8w=s1000);" src="https://lh3.googleusercontent.com/88ya9aocur-91k-r5SyQYfEahxtPkP6b0k3edKViQH_CQp4BGRDaRnAtYuv3_2-KVKOZSamEUUU-RawQ8w=s1000" data-orig-width="1452" data-orig-height="806" >
 +
</div>
 +
</div>
 +
</div></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
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</div>
 +
</div>
 +
 +
 +
 +
 +
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</div>
 +
</div>
 +
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 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-d4fef182-s8ihtzis"  class="master item-box  gallery-box style-d4fef182-t1qz2cig    button-effects btn_hover3    custom  " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-d4fef182-t1qz2cig" data-preview-styleid='style-d4fef182-t1qz2cig' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-d4fef182-t1qz2cig content flex  custom " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-d4fef182-t1qz2cig" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-d4fef182-s8ihtzis" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-d4fef182-t1qz2cig" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/jpruneski8"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-d4fef182-l9phqyhl"  class="sub item-box  page-box style-d4fef182-t1qz2cig          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-d4fef182-t1qz2cig" data-preview-styleid='style-d4fef182-t1qz2cig' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-d4fef182-l9phqyhl" data-preview-style="style-d4fef182-t1qz2cig" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-d4fef182-l9phqyhl" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
16 Jun 2017<br>
+
<div id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
−
WO RG MM PB
+
<!-- VIDEO TEMPLATE -->
−
<ul>
+
−
<li>Determined design of media reservoirs to be conical flasks  
+
−
<li>Explored pre-made MEA vendors
+
−
</ul>
+
  
−
19 Jun 2017<br>
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
−
WO RG MM PB
+
−
<ul>
+
−
<li>Ordered MEAs from Multichannel Systems
+
−
<li>Researched on systems to control the peristaltic pump from the computer
+
−
</ul>
+
  
−
20 Jun 2017<br>
 
−
WO RG MM PB
 
−
<ul>
 
−
<li>Find tubing for bioreactor and pump
 
−
</ul>
 
  
−
21 Jun 2017<br>
+
<!-- MAP TEMPLATE -->
−
WO RG MM PB
+
−
<ul>
+
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
−
<li>Designed media reservoirs in Inventor, obtaining a cubic appearance, as opposed to the original conical tube appearance
+
−
</ul>
+
  
−
22 Jun 2017<br>
+
<!-- RAW TEMPLATE -->
−
WO RG MM PB
+
−
<ul>
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
−
<li>Ordered tubing
+
−
</ul>
+
  
−
26 Jun 2017<br>
+
</div>
−
WO RG MM PB
+
−
<ul>
+
</div>
−
<li>Redesigned clamp system for pins using a platform and screws system
+
−
<li>Revised bioreactor design to have internal channels in one side of the bioreactor
+
−
</ul>
+
  
−
27 Jun 2017<br>
+
</div>
−
WO RG MM PB
+
</div>
−
<ul>
+
<div class="text-side shrinker-parent">
−
<li>Inserted a conical steeple for the bubble trap
+
<div class="vertical-aligner">
−
<li>Made minor modifications to bioreactor design
+
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
−
</ul>
+
<div class="draggable-div-holder"><div id="vbid-d4fef182-0ipm4six-wrapper" class="draggable-pic-wrapper removable-parent">
 +
<div id="vbid-d4fef182-0ipm4six-holder"  class="preview-image-holder draggable-pic-holder removable-parent" data-menu-name="DRAGGABLE_PIC_HOLDER">
 +
<div  id="vbid-d4fef182-0ipm4six" class="draggable-pic preview-element  magic-circle-holder load-high-res" data-menu-name="PREVIEW_DRAGGABLE_PIC" style="background-image:url(https://lh3.googleusercontent.com/ON8D5NvqSjQqSB242cN86UVCNjbcePNyfccUwsEDp9CrK34ExlhOQgTonuh-VmGa8xV3fMCPdSpsQ-AZ4g=s1000);" src="https://lh3.googleusercontent.com/ON8D5NvqSjQqSB242cN86UVCNjbcePNyfccUwsEDp9CrK34ExlhOQgTonuh-VmGa8xV3fMCPdSpsQ-AZ4g=s1000" data-orig-width="1456" data-orig-height="567" >
 +
</div>
 +
</div>
 +
</div></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
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</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-81b639d6-awzas0d2"  class="master item-box  gallery-box style-81b639d6-sicx2q2b    button-effects btn_hover3    custom  " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-81b639d6-sicx2q2b" data-preview-styleid='style-81b639d6-sicx2q2b' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-81b639d6-sicx2q2b content flex  custom " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-81b639d6-sicx2q2b" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-81b639d6-awzas0d2" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-81b639d6-sicx2q2b" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/jpruneski8"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-81b639d6-mjxnkbik"  class="sub item-box  page-box style-81b639d6-sicx2q2b          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-81b639d6-sicx2q2b" data-preview-styleid='style-81b639d6-sicx2q2b' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-81b639d6-mjxnkbik" data-preview-style="style-81b639d6-sicx2q2b" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-81b639d6-mjxnkbik" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
30 Jun 2017<br>
+
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
−
WO RG
+
<!-- VIDEO TEMPLATE -->
−
<ul>
+
−
<li>Inserted threading into media reservoirs
+
−
<li>Made measurements of ordered o-rings to be fitted
+
−
</ul>
+
  
−
3 Jul 2017<br>
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
−
WO RG
+
−
<ul>
+
−
<li>Printed out models to help test fittings for the O-ring
+
−
</ul>
+
  
−
5 Jul 2017<br>
 
−
WO RG
 
−
<ul>
 
−
<li>Printed array to narrow down sizing for O-Ring
 
−
<li>Modified media reservoir due to space constraints
 
−
<li>Printed model MEAs to test pin setup and general fittings
 
−
</ul>
 
  
−
7 Jul 2017<br>
+
<!-- MAP TEMPLATE -->
−
WO RG
+
−
<ul>
+
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
−
<li>Modified media reservoir for better fluid flow
+
−
</ul>
+
  
−
10 Jul 2017<br>
+
<!-- RAW TEMPLATE -->
−
WO RG
+
−
<ul>
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
−
<li>Printed first part of bioreactor
+
−
</ul>
+
  
−
12 Jul 2017<br>
+
</div>
−
WO RG
+
−
<ul>
+
</div>
−
<li>Adjusted dimensions and reprinted first part of bioreactor
+
−
</ul>
+
  
−
13 Jul 2017<br> WO RG
+
</div>
−
<ul>
+
</div>
−
<li>Adjusted dimensions and printed a third version of the first part of bioreactor</li>
+
<div class="text-side shrinker-parent">
−
</ul>
+
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"><div id="vbid-81b639d6-jtlbmbjp-wrapper" class="draggable-pic-wrapper removable-parent">
 +
<div id="vbid-81b639d6-jtlbmbjp-holder"  class="preview-image-holder draggable-pic-holder removable-parent" data-menu-name="DRAGGABLE_PIC_HOLDER">
 +
<div  id="vbid-81b639d6-jtlbmbjp" class="draggable-pic preview-element  magic-circle-holder load-high-res" data-menu-name="PREVIEW_DRAGGABLE_PIC" style="background-image:url(https://lh3.googleusercontent.com/Ym1zx6miHkywz4JJEe9i9kEcudYTEQ0z6LuNzbgR96ubXMcW8S_GvU4Ee_1pbpv-VzF0J7MltGfFv-8ldL8=s1000);" src="https://lh3.googleusercontent.com/Ym1zx6miHkywz4JJEe9i9kEcudYTEQ0z6LuNzbgR96ubXMcW8S_GvU4Ee_1pbpv-VzF0J7MltGfFv-8ldL8=s1000" data-orig-width="1455" data-orig-height="755" >
 +
</div>
 +
</div>
 +
</div></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
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 +
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 +
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</div>
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<div class="helper-div middle-center" >
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<!-- <div class="benet" style="min-height:inherit;"></div> -->
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<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
14 Jul 2017<br> WO RG
+
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
−
<ul>
+
<!-- VIDEO TEMPLATE -->
−
<li>Printed array to determine necessary dimensions to fit luer locks </li>
+
−
</ul>
+
  
−
17 Jul 2017<br> WO RG
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
−
<ul>
+
−
<li>Applied dimension changes to bioreactor design and reprinted the first part of bioreactor</li>
+
−
</ul>
+
  
−
18 Jul 2017<br> WO RG
 
−
<ul>
 
−
<li>Determined screw size and lengths needed to apply appropriate pressure on pins</li>
 
−
</ul>
 
  
−
19 Jul 2017<br> WO RG
+
<!-- MAP TEMPLATE -->
−
<ul>
+
−
<li>Printed out top and bottom of bioreactor and clamp</li>
+
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
−
<li>Perforated MEA Bioreactor modified</li>
+
−
<li>Verified operation of the pump</li>
+
−
<li>Tested calibration of pump</li>
+
−
</ul>
+
  
−
20 Jul 2017<br> WO RG
+
<!-- RAW TEMPLATE -->
−
<ul>
+
−
<li>Printed bioreactor top in Dental SG and media reservoir in clear
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
−
<li>Tested perfusion system with bioreactor and MEA to test for leakage and overall flow
+
−
</ul>
+
  
−
21 Jul 2017<br> WO RG
+
</div>
−
<ul>
+
−
<li>Printed bioreactor bottom in Dental SG and waste reservoir in clear
+
</div>
−
<li>Revised bioreactor design for better hole sizing and placement
+
−
<li>Tested perfusion system with bioreactor, media reservoir, and MEA to test for leakage and overall flow
+
−
<li>Redesigned reservoir for more efficient drainage
+
−
</ul>
+
  
−
24 Jul 2017<br> WO RG
+
</div>
−
<ul>
+
</div>
−
<li>Printed bioreactor top in Dental SG
+
<div class="text-side shrinker-parent">
−
<li>Tested perfusion system with all components to test for leakage and overall flow
+
<div class="vertical-aligner">
−
</ul>
+
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"><div id="vbid-08054b31-mm5iya37-wrapper" class="draggable-pic-wrapper removable-parent">
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 +
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 +
</div>
 +
</div>
 +
</div></div>
 +
<div class="preview-content-holder shrinker-content">
 +
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<!--  BY SPECIFIC ORDER -->
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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<div class="page-wrapper item-wrapper ">
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-796d2780-lb4wfnuc" data-preview-style="style-796d2780-zbcbyf6s" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-796d2780-lb4wfnuc" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
25 Jul 2017<br> WO RG
+
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
−
<ul>
+
<!-- VIDEO TEMPLATE -->
−
<li>Printed both reservoirs in Dental SG
+
−
<li>Tested perfusion system with Dental SG bioreactor top/bottom and MEA to test for leakage and overall flow
+
−
<li>Cured bioreactor pieces in UV
+
−
</ul>
+
  
−
26 Jul 2017<br> WO RG
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
−
<ul>
+
−
<li>Performed dry run with cured pieces
+
−
<li>Autoclaved epoxy-containing piece
+
−
</ul>
+
  
−
27 Jul 2017<br> WO RG
 
−
<ul>
 
−
<li>Autoclaved entire perfusion/bioreactor system
 
−
</ul>
 
  
−
2 August 2017<br> WO RG
+
<!-- MAP TEMPLATE -->
−
<ul>
+
−
<li>Enclosed MEA with transfected cells in between top and bottom piece of bioreactor</li>
+
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
−
<li>Placed in incubator</li>
+
−
</ul>
+
  
−
3 August 2017<br> WO RG
+
<!-- RAW TEMPLATE -->
−
<ul>
+
−
<li>Ran first trial: recorded impedance, started recording voltage at 1,000Hz and injected 0.5mL of 2mM 1-hexanol olfactant, waited 7 minutes and inject 0.5mL more of olfactant, waited 7 minutes, stopped recording, pumped in 1mL of fresh media</li>
+
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
−
<li>Bioreactor and reservoirs were placed back in incubator</li>
+
−
</ul>
+
  
−
4 August 2017<br>WO RG
+
</div>
−
<ul>
+
−
<li>Ran two trials:recorded impedance, started recording voltage at 20,000Hz and injected 0.5mL of 2mM 1-hexanol olfactant, after a total of 1 minute stopped recording, pumped out olfactant with 1mL of fresh media</li>
+
</div>
−
<li>ran an additional trial without recording voltage reading data, analyzed data for a total of 10 minutes</li>
+
−
<li>ran an additional trial without recording voltage reading data injecting 20mM of olfactant, analyzed data for a total of 10 minutes</li>
+
−
</ul> 
+
−
<a name="Programming and Electronics"></a> <br>
+
−
<h3>Programming and Electronics (Bioreactor focus)</h3>
+
  
−
23 May 2017 <br>
+
</div>
−
WO
+
</div>
−
<ul>
+
<div class="text-side shrinker-parent">
−
<li>Introduced to labview - use to take voltage readings from electrodes
+
<div class="vertical-aligner">
−
<li>Determined to set peak threshold to +/- 2 SD of base activity
+
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
−
</ul>
+
<div class="draggable-div-holder"><div id="vbid-796d2780-nyuhkbt2-wrapper" class="draggable-pic-wrapper removable-parent">
−
 
+
<div id="vbid-796d2780-nyuhkbt2-holder"  class="preview-image-holder draggable-pic-holder removable-parent" data-menu-name="DRAGGABLE_PIC_HOLDER">
−
25 May 2017 <br>
+
<div  id="vbid-796d2780-nyuhkbt2" class="draggable-pic preview-element  magic-circle-holder load-high-res" data-menu-name="PREVIEW_DRAGGABLE_PIC" style="background-image:url(https://lh3.googleusercontent.com/1vffzX1dkHnOSU3TpVhke8yHp465uIZYhKPECc0dkNUAJ7EIjq0YKvlb1R7PbFgSRk0bEIzpf-0s1nbybA=s1000);" src="https://lh3.googleusercontent.com/1vffzX1dkHnOSU3TpVhke8yHp465uIZYhKPECc0dkNUAJ7EIjq0YKvlb1R7PbFgSRk0bEIzpf-0s1nbybA=s1000" data-orig-width="1466" data-orig-height="565" >
−
WO
+
</div>
−
<ul>
+
</div>
−
<li>Built time-averaging method to determine frequency and amplitude of peaks
+
</div></div>
−
<li>Generated preliminary statistics for data
+
<div class="preview-content-holder shrinker-content">
−
<li>Built code to write to a file
+
−
</ul>
+
<!--  BY SPECIFIC ORDER -->
−
 
+
−
30 May 2017 <br>
+
−
WO
+
−
<ul>
+
−
<li>Inserted method to adjust thresholds before data collection
+
−
</ul>
+
−
 
+
</div>
−
29 Jun 2017 <br>
+
</div>
−
WO
+
</div>
−
<ul>
+
</div>
−
<li>Adjusted labview program to read in data from electrodes using Intan codes (as opposed to theoretical inputs)
+
</div>
−
<li>Finished labview program - can select filename/type
+
</div>
−
</ul>
+
</div>
−
 
+
</div>
−
12 Jun 2017 <br>
+
</div>
−
WO
+
<!-- LAYOUT SETTINGS -->
−
<ul>
+
<div class="layout-settings" style="display:none;" data-type="multi"></div>
−
<li>Implemented impedance program from Intan to determine the presence of cells on electrodes
+
−
</ul>
+
</div>
−
 
+
</div>
−
13 Jul 2017<br> WO RG
+
−
<ul>  
+
−
<li>Soldered pins to electrode adapter boards</li>
+
−
</ul>
+
−
 
+
−
19 Jul 2017<br> WO RG
+
</div>
−
<ul>
+
</div>
−
<li>Tested program that ran impedance tests and voltage readings</li>
+
−
<li>Soldered more electrode adapters</li>
+
−
</ul>
+
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
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<div class="light-box-wrapper  space-layer" style="display:none;">
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<div class="light-box-image-holder">
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<div class="light-box-image animated">
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 +
<div class="lightbox-text-wrapper ">
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<div class="lightbox-text-holder animated">
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<div class="lightbox-title"></div>
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<div class="lightbox-subtitle"></div>
 +
<div id="paginator" style="display:none;">
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</div>
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</div>
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</div>
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</div>
 +
    <img src="https://lh3.googleusercontent.com/EWqW7DEI4kOTRMLjK2-ObFHp-EYBt5apFYZ1LVFAhLtTLjigCRfx5hCCTKbIjIm68VQ00p9twloHJ9w8=s50" class="download-gallery-btn clickable" style="display: none;">
 +
<img src="https://lh3.googleusercontent.com/TgRyMQvJ3_h9RmOnu7AlhIE7NLOOBsRoBounARrs8fQv8HCRPaFtpBneSqJOSZpI6l7He_bAZKN179JBig=s50" class="close-lightbox-btn clickable" style="opacity: 1;">
 +
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 +
<img src="https://lh3.googleusercontent.com/9rwgVnDglPdPFugSu98fhDmxzjXC9KovZ_7BuHkXPIv6jvg9S96flGnhL_e4y8mIpPpZQstfqEV-WitY=s50" class="lightbox-arrow lightbox-right clickable top-layer" >
 +
</div>
  
−
3 August 2017<br>WO RG
+
</div>
−
<ul>
+
−
<li>Soldered pins to electrode adapter board ensuring to have pins on the ground and reference channels on adapter board</li>
+
<script src="//www.imcreator.com/js/lightbox.js?v=1.4.1f"  type="text/javascript"></script>
−
</ul>
+
<script src="//www.imcreator.com/js/spimeengine.js?v=1.4.1f"  type="text/javascript"></script>
 +
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<!--  SHOPROCKET  ECOMMERCE  -->
 +
<script>
 +
var JSR = jQuery.noConflict();
 +
var $ = jQuery.noConflict();
 +
</script>
 +
 +
<input type="hidden" name="sr-companyid" id="sr-companyid" value="">
 +
</body>
  
−
4 August 2017<br>WO RG
+
−
<ul>
+
−
<li>Set voltage reading program to read at 20,000Hz</li>
+
−
</ul>
+
 
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Revision as of 03:02, 1 November 2017

<!DOCTYPE html> NOTEBOOK - Demonstrate