Difference between revisions of "Team:USMA-West Point/Notebook"

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{{USMA-West_Point}}
 
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NOTEBOOK - Demonstrate</title>
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Notebook - iGem 01</title>
 
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<h1 id="vbid-b2f224f4-ybp6rqll" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Experiments<br></h1>
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<h3 id="vbid-b2f224f4-ciiltbxs" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Day to Day activity inside the lab<br></h3>
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data-ARRANGER_ITEM_MIN_WIDTH="230"
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data-ARRANGER_ITEMS_PER_PAGE="all"
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<h2 id="vbid-b2f224f4-swvwo2fc" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >January 2017<br></h2>
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<h3 id="vbid-b2f224f4-3axuxfta" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Transformation of Bacteria Cells</h3>
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<ol><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">OR vectors were purchased from OriGene and transfected into XL-10 Gold Ultracompetent cells by heat shocking at 42 degrees Celsius</span></span></span><br></li><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Cells were cultured in LB broth to amplify vectors</span></span></span><br></li><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">DNA gels were run with specific restriction enzymes to determine multiple cloning sites</span></span></span><br></li><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Participants</span></span></span><ol><li class="font_7" style="text-align: justify;"><a data-cke-saved-href="https://www.mcdb.ucla.edu/Research/Banerjee/protocols/maxiprep--Qiagen.pdf" href="https://www.mcdb.ucla.edu/Research/Banerjee/protocols/maxiprep--Qiagen.pdf"></a><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Dr. Alexander Mitropoulos, Dr. Ken Wickiser, Dr. Kamil Woronowicz, 2LT Dillon Macky, 2LT Ishmael Raheem, CDT Jason Hug, CDT Elizabeth Huuki, CDT Channah Mills, CDT Matthew McDonough, Isaac Dabkowski</span></span></span><br></li></ol></li></ol>
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<div  id="element-7e6ba4af3aa01cf" class="draggable-pic preview-element  magic-circle-holder load-high-res" data-menu-name="PREVIEW_DRAGGABLE_PIC" style="background-image:url(https://lh3.googleusercontent.com/aMY9ftvkOygcdEhYC8YrK74Lh6qpcpJbrQO38tKSrvBvYfmyk28s0WYvefinyAQUxtXJF04pgso523r6y_A=s1000);" src="https://lh3.googleusercontent.com/aMY9ftvkOygcdEhYC8YrK74Lh6qpcpJbrQO38tKSrvBvYfmyk28s0WYvefinyAQUxtXJF04pgso523r6y_A=s1000" data-orig-width="1463" data-orig-height="536" >
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<h2 id="vbid-bb2e3d32-e7fsajn5" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >February 2017<br></h2>
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<br class="lower-line-break" />
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<div class="preview-subtitle-holder removable-parent order-handle">
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<h3 id="vbid-bb2e3d32-wtn44f6j" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Western Blotting</h3>
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</div>
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<br class="lower-line-break" />
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<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
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<div id="vbid-bb2e3d32-dpy97wsn" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<ol><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">HT-22 cells were lysed in RIPA buffer with phosphate and protease inhibitors</span></span></span><br></p></li><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Protein concentration was measured for each OR</span></span></span><br></p></li><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Proteins were prepared with Laemmle sample buffer before loading into 4% Bis-Tris gels to measure protein purity and size</span></span></span><br></p></li><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Participants</span></span></span><ol><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Dr. Alexander Mitropoulos, Dr. Ken Wickiser, Dr. Kamil Woronowicz, 2LT Dillon Macky, 2LT Ishmael Raheem, CDT Jason Hug, CDT Elizabeth Huuki, CDT Channah Mills, CDT Matthew McDonough, Isaac Dabkowski</span></span></span><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">​</span></span></span><br></li></ol></li></ol>
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<div  id="vbid-c9477c3f-hpnty17e"  class="master item-box  gallery-box style-c9477c3f-db96lxak   button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-c9477c3f-db96lxak" data-preview-styleid='style-c9477c3f-db96lxak' data-preset-type-id="TEXT_BLOCK">
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
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<div  class="sub container style-d3269007-gm2iekds content flex custom " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-d3269007-gm2iekds" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-d3269007-nqff64ja" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-d3269007-gm2iekds" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/jpruneski8"  >
+
<div  class="sub container style-c9477c3f-db96lxak content flex   " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-c9477c3f-db96lxak" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-c9477c3f-hpnty17e" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-c9477c3f-db96lxak" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 
<!-- ARRANGER SETTINGS -->
 
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<div  id="vbid-d3269007-ovjtodbr"  class="sub item-box  page-box style-d3269007-gm2iekds         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-d3269007-gm2iekds" data-preview-styleid='style-d3269007-gm2iekds' data-preset-type-id="UNRESOLVED">
+
<div  id="vbid-c9477c3f-pqgwwgha"  class="sub item-box  page-box style-c9477c3f-db96lxak         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-c9477c3f-db96lxak" data-preview-styleid='style-c9477c3f-db96lxak' data-preset-type-id="UNRESOLVED">
 
 
 
<div class="page-wrapper item-wrapper ">
 
<div class="page-wrapper item-wrapper ">
 
 
−
<div class="item-content leaf multi_layout page content -container" data-self="vbid-d3269007-ovjtodbr" data-preview-style="style-d3269007-gm2iekds" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-d3269007-ovjtodbr" data-bgimg="">
+
<div class="item-content leaf multi_layout page content -container" data-self="vbid-c9477c3f-pqgwwgha" data-preview-style="style-c9477c3f-db96lxak" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="3264" data-orig-thumb-width="2448" data-vbid="vbid-c9477c3f-pqgwwgha" data-bgimg="https://lh3.googleusercontent.com/QXojKjlZ5V8Irr8oAS8ejVLhmSv_64xDj_-N5yQ2GeexGs1rZcFVgiJNhnSWQ2Zxj3vFiz5P0yy37IvUwZY">
 
<div  class="multi-container preview image-cover" >
 
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<!-- <div class="benet" style="min-height:inherit;"></div> -->
 
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<div  id="element-a27df69d08403e5" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/QXojKjlZ5V8Irr8oAS8ejVLhmSv_64xDj_-N5yQ2GeexGs1rZcFVgiJNhnSWQ2Zxj3vFiz5P0yy37IvUwZY=s300);"  data-orig-width="2448" data-orig-height="3264" >
 
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<div id="vbid-d3269007-0aey4cii-holder"  class="preview-image-holder draggable-pic-holder removable-parent" data-menu-name="DRAGGABLE_PIC_HOLDER">
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<div  id="vbid-d3269007-0aey4cii" class="draggable-pic preview-element  magic-circle-holder load-high-res" data-menu-name="PREVIEW_DRAGGABLE_PIC" style="background-image:url(https://lh3.googleusercontent.com/8eTEMR5NAG8g62JWH-VQ0gT4ES_tRvQKt0HYpzo6pYnVhSAPXXG11sWPW6p8ds_M6ZK7D2E2lcZjLmvWGA=s1000);" src="https://lh3.googleusercontent.com/8eTEMR5NAG8g62JWH-VQ0gT4ES_tRvQKt0HYpzo6pYnVhSAPXXG11sWPW6p8ds_M6ZK7D2E2lcZjLmvWGA=s1000" data-orig-width="1460" data-orig-height="491" >
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<div class="preview-content-holder shrinker-content">
 
<div class="preview-content-holder shrinker-content">
 
 
 
<!--  BY SPECIFIC ORDER -->
 
<!--  BY SPECIFIC ORDER -->
 +
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<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
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<h2 id="vbid-c9477c3f-y1ukkv8r" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >March 2017<br></h2>
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<br class="lower-line-break" />
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<div class="preview-subtitle-holder removable-parent order-handle">
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<h3 id="vbid-c9477c3f-o0cafyw3" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Confocal Microscopy</h3>
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<br class="lower-line-break" />
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<br class="upper-line-break" />
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<div class="preview-body-holder removable-parent order-handle">
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<div id="vbid-c9477c3f-rhbmmfpx" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<ol><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">HT-22 cells were fixed with 4% paraformaldehyde and immunohistochemistry with antibodies responsible for individual OR binding followed by secondary antibodies for visualization</span></span></span><br></p></li><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Participants</span></span></span><ol><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Dr. Alexander Mitropoulos, Dr. Ken Wickiser, Dr. Kamil Woronowicz, 2LT Dillon Macky, 2LT Ishmael Raheem, CDT Jason Hug, CDT Elizabeth Huuki, CDT Channah Mills, CDT Matthew McDonough, Isaac Dabkowski</span></span></span><br></li></ol></li></ol>
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<div  id="vbid-b3e50235-zvukijf2"  class="master item-box  gallery-box style-b3e50235-yb8q8j7l   button-effects btn_hover3    custom  " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-b3e50235-yb8q8j7l" data-preview-styleid='style-b3e50235-yb8q8j7l' data-preset-type-id="TEXT_BLOCK">
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<div  id="vbid-df58bf33-hpnty17e"  class="master item-box  gallery-box style-df58bf33-db96lxak   button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-df58bf33-db96lxak" data-preview-styleid='style-df58bf33-db96lxak' data-preset-type-id="TEXT_BLOCK">
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
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<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 
 
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<div  class="sub container style-b3e50235-yb8q8j7l content flex custom " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-b3e50235-yb8q8j7l" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-b3e50235-zvukijf2" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-b3e50235-yb8q8j7l" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/jpruneski8"  >
+
<div  class="sub container style-df58bf33-db96lxak content flex   " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-df58bf33-db96lxak" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-df58bf33-hpnty17e" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-df58bf33-db96lxak" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 
<!-- ARRANGER SETTINGS -->
 
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<div  id="vbid-b3e50235-ne8j6nq3"  class="sub item-box  page-box style-b3e50235-yb8q8j7l         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-b3e50235-yb8q8j7l" data-preview-styleid='style-b3e50235-yb8q8j7l' data-preset-type-id="UNRESOLVED">
+
<div  id="vbid-df58bf33-pqgwwgha"  class="sub item-box  page-box style-df58bf33-db96lxak         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-df58bf33-db96lxak" data-preview-styleid='style-df58bf33-db96lxak' data-preset-type-id="UNRESOLVED">
 
 
 
<div class="page-wrapper item-wrapper ">
 
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−
<div class="item-content leaf multi_layout page content -container" data-self="vbid-b3e50235-ne8j6nq3" data-preview-style="style-b3e50235-yb8q8j7l" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-b3e50235-ne8j6nq3" data-bgimg="">
+
<div class="item-content leaf multi_layout page content -container" data-self="vbid-df58bf33-pqgwwgha" data-preview-style="style-df58bf33-db96lxak" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-df58bf33-pqgwwgha" data-bgimg="">
 
<div  class="multi-container preview image-cover" >
 
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<div id="vbid-b3e50235-3ydgf1h8-holder"  class="preview-image-holder draggable-pic-holder removable-parent" data-menu-name="DRAGGABLE_PIC_HOLDER">
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<div  id="vbid-b3e50235-3ydgf1h8" class="draggable-pic preview-element  magic-circle-holder load-high-res" data-menu-name="PREVIEW_DRAGGABLE_PIC" style="background-image:url(https://lh3.googleusercontent.com/kuyajy7-DAfnr0vpO_IApEksrdmBI_p0IVrDGWBamNMcpYKltAWhRdYYTtLXSlwA9aJfTOe4nBJKq__Vxg=s1000);" src="https://lh3.googleusercontent.com/kuyajy7-DAfnr0vpO_IApEksrdmBI_p0IVrDGWBamNMcpYKltAWhRdYYTtLXSlwA9aJfTOe4nBJKq__Vxg=s1000" data-orig-width="1463" data-orig-height="408" >
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<div class="preview-title-holder removable-parent order-handle">
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<h2 id="vbid-df58bf33-y1ukkv8r" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >April 2017<br></h2>
 +
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</div>
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<br class="lower-line-break" />
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<h3 id="vbid-df58bf33-o0cafyw3" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Action Potential Measurements</h3>
 +
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</div>
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<div class="preview-body-holder removable-parent order-handle">
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<div id="vbid-df58bf33-rhbmmfpx" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
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<ol><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"></span></span></span><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Nucleofected HT-22 cells were stimulated with individual odorants</span></span></span><br></p></li><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">The neurons generated action potentials that were measured via a microelectrode array</span></span></span><br></p></li><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"></span></span></span><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Participants</span></span></span><ol><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Dr. Alexander Mitropoulos, Dr. Ken Wickiser, Dr. Kamil Woronowicz, 2LT Dillon Macky, 2LT Ishmael Raheem, CDT Jason Hug, CDT Elizabeth Huuki, CDT Channah Mills, CDT Matthew McDonough, Isaac Dabkowski</span></span></span><br></li></ol></li></ol>
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<div  class="sub container style-3d4ff3cd-2p5gmo8r content flex   " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-3d4ff3cd-2p5gmo8r" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-3d4ff3cd-p2ohw055" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-3d4ff3cd-2p5gmo8r" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 
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<div  id="vbid-3d4ff3cd-o0ba9iw8"  class="sub item-box  page-box style-3d4ff3cd-2p5gmo8r         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-3d4ff3cd-2p5gmo8r" data-preview-styleid='style-3d4ff3cd-2p5gmo8r' data-preset-type-id="UNRESOLVED">
 
 
 
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-3d4ff3cd-o0ba9iw8" data-preview-style="style-3d4ff3cd-2p5gmo8r" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="2592" data-orig-thumb-width="3872" data-vbid="vbid-3d4ff3cd-o0ba9iw8" data-bgimg="https://lh3.googleusercontent.com/nQjKEktNrFksk3oBSUBL3If8TXXgu24pvw-GIvFjuubu69TyFY8NZ70THN-wjFOgWzmB74i6JQav4HMj">
 
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<h2 id="vbid-3d4ff3cd-kekeilno" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >May 2017<br></h2>
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<ol><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"></span></span></span><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Presented research at United States Military Academy Projects Day, a day where students present projects concerning research in various fields</span></span></span><br></p></li><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Reports put together for eNOSE</span></span></span><br></p></li><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"></span></span></span><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Participants</span></span></span><ol><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Dr. Alexander Mitropoulos, Dr. Ken Wickiser, Dr. Kamil Woronowicz, 2LT Dillon Macky, 2LT Ishmael Raheem, CDT Jason Hug, CDT Elizabeth Huuki, CDT Channah Mills, CDT Matthew McDonough, Isaac Dabkowski</span></span></span><br></li></ol></li></ol>
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<div  class="sub container style-252908aa-svxsoqiw content flex   " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-252908aa-svxsoqiw" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-252908aa-g32bqwa3" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-252908aa-svxsoqiw" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 
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<div  id="vbid-252908aa-p6hwyodf"  class="sub item-box  page-box style-252908aa-svxsoqiw         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-252908aa-svxsoqiw" data-preview-styleid='style-252908aa-svxsoqiw' data-preset-type-id="UNRESOLVED">
 
 
 
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<h2 id="vbid-252908aa-x6d0iiuy" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >June 2017<br></h2>
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<h3 id="vbid-252908aa-ywwepk8k" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Further Trials and Biosensor Design</h3>
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<ol><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"></span></span></span><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Cells amplified</span></span></span><br></p></li><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Focused on designing a biosensor</span></span></span></p><ul><li style="line-height: 1.8em; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Bioreactor</span></span></span></li><li style="line-height: 1.8em; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Microelectrode array (MEA)</span></span></span></li><li style="line-height: 1.8em; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Perfusion system</span></span></span></li><li style="line-height: 1.8em; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Signal Processor</span></span></span><br></li></ul></li><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"></span></span></span><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Participants</span></span></span><ol><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Dr. Kirk Pirlo, Dr. Joel Gaston, Winston Ou, Rayonna Gordon, CDT Angela Marsh, CDT Kanak Pal, CDT Payton Boylston, CDT Matthew McDonough</span></span></span><br></li></ol></li></ol>
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 +
<div id="no-image" class="stripe-background load-high-res " ></div>
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<div  class="sub container style-11f2600f-2p5gmo8r content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-11f2600f-2p5gmo8r" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-11f2600f-p2ohw055" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-11f2600f-2p5gmo8r" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
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<div class="arranger-settings" style="display:none;"
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data-ARRANGER_TYPE="flex"
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data-ARRANGER_COLS="0"
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data-ARRANGER_ITEM_MAX_WIDTH="400"
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data-ARRANGER_ITEM_MIN_WIDTH="230"
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data-ARRANGER_ITEM_RATIO="1"
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data-ARRANGER_ITEM_SPACING="20"
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data-ARRANGER_ITEMS_PER_PAGE="all"
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data-ARRANGER_ORDER_TYPE="regular"
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data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
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<div class="layout-settings" style="display:none;" data-type="multi"></div>
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<div id="children">
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<div id="items-holder-wrapper">
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<div  id="vbid-11f2600f-o0ba9iw8"  class="sub item-box  page-box style-11f2600f-2p5gmo8r          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-11f2600f-2p5gmo8r" data-preview-styleid='style-11f2600f-2p5gmo8r' data-preset-type-id="UNRESOLVED">
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<div class="page-wrapper item-wrapper ">
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-11f2600f-o0ba9iw8" data-preview-style="style-11f2600f-2p5gmo8r" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="3264" data-orig-thumb-width="2448" data-vbid="vbid-11f2600f-o0ba9iw8" data-bgimg="https://lh3.googleusercontent.com/AluMrQaeiwQMRltyNlt7xgSZuwHBVkR56wCu3pFGPgFfi2XAPso-IQt7lgTfiRHQ4ivON3Ie3T8iVQPOtA">
 +
<div  class="multi-container preview image-cover" >
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<div class="Picture item-preview">
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<!-- <div class="benet" style="min-height:inherit;"></div> -->
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<div id="element-27405ce1421a02a-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 
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<div  id="element-27405ce1421a02a" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/AluMrQaeiwQMRltyNlt7xgSZuwHBVkR56wCu3pFGPgFfi2XAPso-IQt7lgTfiRHQ4ivON3Ie3T8iVQPOtA=s300);"  data-orig-width="2448" data-orig-height="3264" >
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<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 
<div class="preview-content-holder shrinker-content">
 
<div class="preview-content-holder shrinker-content">
 
 
 
<!--  BY SPECIFIC ORDER -->
 
<!--  BY SPECIFIC ORDER -->
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<div class="preview-title-holder removable-parent order-handle">
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<h2 id="vbid-11f2600f-kekeilno" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >July 2017<br></h2>
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 +
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<h3 id="vbid-11f2600f-bb0e8gwp" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Finalizing Design</h3>
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<br class="lower-line-break" />
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<br class="upper-line-break" />
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<div class="preview-body-holder removable-parent order-handle">
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<div id="vbid-11f2600f-4adbl1bh" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<ol><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"></span></span></span><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Finalized biosensor design as necessary parts were delivered</span></span></span><br></p></li><li style="line-height: 1.8em; text-align: left;">Prepared for trials with updated biosensor</li><li style="line-height: 1.8em; text-align: left;">Additional transformations<span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"></span></span></span><br></li><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"></span></span></span><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Participants</span></span></span><ol><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Dr. Kirk Pirlo, Dr. Joel Gaston, Winston Ou, Rayonna Gordon, Dr. Alex Mitropoulos</span></span></span><br></li></ol></li></ol>
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<div  id="vbid-81b639d6-awzas0d2"  class="master item-box  gallery-box style-81b639d6-sicx2q2b   button-effects btn_hover3    custom  " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-81b639d6-sicx2q2b" data-preview-styleid='style-81b639d6-sicx2q2b' data-preset-type-id="TEXT_BLOCK">
+
<div  id="vbid-329bdd0e-4vsb460y"  class="master item-box  gallery-box style-329bdd0e-q8mrvipb   button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-329bdd0e-q8mrvipb" data-preview-styleid='style-329bdd0e-q8mrvipb' data-preset-type-id="TEXT_BLOCK">
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
<div id="no-image" class="stripe-background load-high-res " ></div>
 
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<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 
 
−
<div  class="sub container style-81b639d6-sicx2q2b content flex custom " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-81b639d6-sicx2q2b" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-81b639d6-awzas0d2" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-81b639d6-sicx2q2b" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/jpruneski8"  >
+
<div  class="sub container style-329bdd0e-q8mrvipb content flex   " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-329bdd0e-q8mrvipb" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-329bdd0e-4vsb460y" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-329bdd0e-q8mrvipb" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 
<!-- ARRANGER SETTINGS -->
 
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<div  id="vbid-81b639d6-mjxnkbik"  class="sub item-box  page-box style-81b639d6-sicx2q2b         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-81b639d6-sicx2q2b" data-preview-styleid='style-81b639d6-sicx2q2b' data-preset-type-id="UNRESOLVED">
+
<div  id="vbid-329bdd0e-hlefawyr"  class="sub item-box  page-box style-329bdd0e-q8mrvipb         " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-329bdd0e-q8mrvipb" data-preview-styleid='style-329bdd0e-q8mrvipb' data-preset-type-id="UNRESOLVED">
 
 
 
<div class="page-wrapper item-wrapper ">
 
<div class="page-wrapper item-wrapper ">
 
 
−
<div class="item-content leaf multi_layout page content -container" data-self="vbid-81b639d6-mjxnkbik" data-preview-style="style-81b639d6-sicx2q2b" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-81b639d6-mjxnkbik" data-bgimg="">
+
<div class="item-content leaf multi_layout page content -container" data-self="vbid-329bdd0e-hlefawyr" data-preview-style="style-329bdd0e-q8mrvipb" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-329bdd0e-hlefawyr" data-bgimg="">
 
<div  class="multi-container preview image-cover" >
 
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−
<div class="draggable-div-holder"><div id="vbid-81b639d6-jtlbmbjp-wrapper" class="draggable-pic-wrapper removable-parent">
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−
<div id="vbid-81b639d6-jtlbmbjp-holder" class="preview-image-holder draggable-pic-holder removable-parent" data-menu-name="DRAGGABLE_PIC_HOLDER">
+
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−
<div id="vbid-81b639d6-jtlbmbjp" class="draggable-pic preview-element magic-circle-holder load-high-res" data-menu-name="PREVIEW_DRAGGABLE_PIC" style="background-image:url(https://lh3.googleusercontent.com/Ym1zx6miHkywz4JJEe9i9kEcudYTEQ0z6LuNzbgR96ubXMcW8S_GvU4Ee_1pbpv-VzF0J7MltGfFv-8ldL8=s1000);" src="https://lh3.googleusercontent.com/Ym1zx6miHkywz4JJEe9i9kEcudYTEQ0z6LuNzbgR96ubXMcW8S_GvU4Ee_1pbpv-VzF0J7MltGfFv-8ldL8=s1000" data-orig-width="1455" data-orig-height="755" >
+
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<!--  BY SPECIFIC ORDER -->
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<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
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<h2 id="vbid-329bdd0e-vz89kiwg" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >August 2017<br></h2>
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<br class="upper-line-break" />
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<div class="preview-subtitle-holder removable-parent order-handle">
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<h3 id="vbid-329bdd0e-jg7x8bnq" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">Trials with Updated Device</h3>
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<br class="upper-line-break" />
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<div class="preview-body-holder removable-parent order-handle">
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<div id="vbid-329bdd0e-2em3oyaf" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<ol><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"></span></span></span><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Ran successful trials with updated biosensor</span></span></span><br></p></li><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Collected more data for device</span></span></span><br></p></li><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"></span></span></span><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Participants</span></span></span><ol><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Dr. Kirk Pirlo, Dr. Joel Gaston, Winston Ou, Rayonna Gordon, Dr. Alex Mitropoulos</span></span></span><br></li></ol></li></ol>
 +
</div>
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<div  id="vbid-b49c473a-iiz4ntn0"  class="master item-box  gallery-box style-b49c473a-xhfftgs0    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-b49c473a-xhfftgs0" data-preview-styleid='style-b49c473a-xhfftgs0' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
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<div  class="sub container style-b49c473a-xhfftgs0 content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-b49c473a-xhfftgs0" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-b49c473a-iiz4ntn0" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-b49c473a-xhfftgs0" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
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<div id="children">
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<div id="items-holder-wrapper">
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<div  id="vbid-b49c473a-tcz6jwws"  class="sub item-box  page-box style-b49c473a-xhfftgs0          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-b49c473a-xhfftgs0" data-preview-styleid='style-b49c473a-xhfftgs0' data-preset-type-id="UNRESOLVED">
 +
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<div class="page-wrapper item-wrapper ">
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<div class="item-content leaf multi_layout page content -container" data-self="vbid-b49c473a-tcz6jwws" data-preview-style="style-b49c473a-xhfftgs0" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="3264" data-orig-thumb-width="2448" data-vbid="vbid-b49c473a-tcz6jwws" data-bgimg="https://lh3.googleusercontent.com/G2aBW4VCh3XH_V4ORO1OqUT0BQG4t8XlC751DlICfhxYXv2mGHmSWqanmCHejjRWB08JrB-h4QXc5zP7">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
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<div class="preview-image-holder">
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<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
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</div>
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<div class="helper-div middle-left" >
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<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
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<div class="vertical-aligner">
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<div id="element-a28a1c708b88dda-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 
 +
<div  id="element-a28a1c708b88dda" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/G2aBW4VCh3XH_V4ORO1OqUT0BQG4t8XlC751DlICfhxYXv2mGHmSWqanmCHejjRWB08JrB-h4QXc5zP7=s300);"  data-orig-width="2448" data-orig-height="3264" >
 +
<!-- VIDEO TEMPLATE -->
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<ol><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;"></span></span></span><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Completed Interlab protocol and collected data</span></span></span><br></p></li><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Developed iGEM plasmid by transfection and amplification</span></span></span></p></li><li style="line-height: 1.8em;"><p class="font_8" style="line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Ran gel of plasmids to evaluate</span></span></span><br></p></li><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Participants</span></span></span><ol><li class="font_7" style="text-align: justify;"><span style="color: rgb(0, 0, 0);"><span style="font-size: 15px;"><span style="font-family: helvetica-w01-light,helvetica-w02-light,sans-serif;">Dr. Alexander Mitropoulos, Dr. Ken Wickiser, Dr. Kamil Woronowicz, Dr. John Cave, CDT Jason Hug, CDT Zachary Andersen, CDT Kylor Keisewetter, CDT Alana Appel, CDT Elizabeth Huuki​, CDT Channah Mills, CDT Matthew McDonough, CDT James Pruneski</span></span></span><br></li></ol></li></ol>
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Revision as of 04:40, 1 November 2017

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USMA-West_Point

<!DOCTYPE html> Notebook - iGem 01

usMA-WEST POINT


Experiments



Day to Day activity inside the lab



January 2017



Transformation of Bacteria Cells



  1. OR vectors were purchased from OriGene and transfected into XL-10 Gold Ultracompetent cells by heat shocking at 42 degrees Celsius
  2. Cells were cultured in LB broth to amplify vectors
  3. DNA gels were run with specific restriction enzymes to determine multiple cloning sites
  4. Participants
    1. Dr. Alexander Mitropoulos, Dr. Ken Wickiser, Dr. Kamil Woronowicz, 2LT Dillon Macky, 2LT Ishmael Raheem, CDT Jason Hug, CDT Elizabeth Huuki, CDT Channah Mills, CDT Matthew McDonough, Isaac Dabkowski


February 2017



Western Blotting



  1. HT-22 cells were lysed in RIPA buffer with phosphate and protease inhibitors

  2. Protein concentration was measured for each OR

  3. Proteins were prepared with Laemmle sample buffer before loading into 4% Bis-Tris gels to measure protein purity and size

  4. Participants
    1. Dr. Alexander Mitropoulos, Dr. Ken Wickiser, Dr. Kamil Woronowicz, 2LT Dillon Macky, 2LT Ishmael Raheem, CDT Jason Hug, CDT Elizabeth Huuki, CDT Channah Mills, CDT Matthew McDonough, Isaac Dabkowski​


March 2017



Confocal Microscopy



  1. HT-22 cells were fixed with 4% paraformaldehyde and immunohistochemistry with antibodies responsible for individual OR binding followed by secondary antibodies for visualization

  2. Participants
    1. Dr. Alexander Mitropoulos, Dr. Ken Wickiser, Dr. Kamil Woronowicz, 2LT Dillon Macky, 2LT Ishmael Raheem, CDT Jason Hug, CDT Elizabeth Huuki, CDT Channah Mills, CDT Matthew McDonough, Isaac Dabkowski


April 2017



Action Potential Measurements



  1. Nucleofected HT-22 cells were stimulated with individual odorants

  2. The neurons generated action potentials that were measured via a microelectrode array

  3. Participants
    1. Dr. Alexander Mitropoulos, Dr. Ken Wickiser, Dr. Kamil Woronowicz, 2LT Dillon Macky, 2LT Ishmael Raheem, CDT Jason Hug, CDT Elizabeth Huuki, CDT Channah Mills, CDT Matthew McDonough, Isaac Dabkowski


May 2017



USMA Projects Day



  1. Presented research at United States Military Academy Projects Day, a day where students present projects concerning research in various fields

  2. Reports put together for eNOSE

  3. Participants
    1. Dr. Alexander Mitropoulos, Dr. Ken Wickiser, Dr. Kamil Woronowicz, 2LT Dillon Macky, 2LT Ishmael Raheem, CDT Jason Hug, CDT Elizabeth Huuki, CDT Channah Mills, CDT Matthew McDonough, Isaac Dabkowski


June 2017



Further Trials and Biosensor Design



  1. Cells amplified

  2. Focused on designing a biosensor

    • Bioreactor
    • Microelectrode array (MEA)
    • Perfusion system
    • Signal Processor
  3. Participants
    1. Dr. Kirk Pirlo, Dr. Joel Gaston, Winston Ou, Rayonna Gordon, CDT Angela Marsh, CDT Kanak Pal, CDT Payton Boylston, CDT Matthew McDonough


July 2017



Finalizing Design



  1. Finalized biosensor design as necessary parts were delivered

  2. Prepared for trials with updated biosensor
  3. Additional transformations
  4. Participants
    1. Dr. Kirk Pirlo, Dr. Joel Gaston, Winston Ou, Rayonna Gordon, Dr. Alex Mitropoulos


August 2017



Trials with Updated Device



  1. Ran successful trials with updated biosensor

  2. Collected more data for device

  3. Participants
    1. Dr. Kirk Pirlo, Dr. Joel Gaston, Winston Ou, Rayonna Gordon, Dr. Alex Mitropoulos


September 2017



Interlab Protocol and iGEM Plasmid



  1. Completed Interlab protocol and collected data

  2. Developed iGEM plasmid by transfection and amplification

  3. Ran gel of plasmids to evaluate

  4. Participants
    1. Dr. Alexander Mitropoulos, Dr. Ken Wickiser, Dr. Kamil Woronowicz, Dr. John Cave, CDT Jason Hug, CDT Zachary Andersen, CDT Kylor Keisewetter, CDT Alana Appel, CDT Elizabeth Huuki​, CDT Channah Mills, CDT Matthew McDonough, CDT James Pruneski


October 2017



Preparation for iGEM



  1. Finalized results

  2. Design and develop parts

  3. Prepared iGEM wiki and presentation

  4. Participants
    1. Dr. Alexander Mitropoulos, Dr. Ken Wickiser, Dr. Kamil Woronowicz, Dr. John Cave, CDT Jason Hug, CDT Zachary Andersen, CDT Kylor Keisewetter, CDT Alana Appel, CDT Elizabeth Huuki​, CDT Channah Mills, CDT Matthew McDonough, CDT James Pruneski


DEPARTMENT OF CHEMISTRY AND LIFE SCIENCE



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TEL: 845.938.3915