Difference between revisions of "Team:USMA-West Point/Notebook"
(Begin transcription of notes pertaining to the design of the bioreactor, MEA. MEA: Last Update 1 Jun 2017, Bioreactor: 8 Jun 2017, Fluidics: 9 Jun 2017) |
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| Line 66: | Line 66: | ||
1 Jun 2017 <br> WO RM AM KP | 1 Jun 2017 <br> WO RM AM KP | ||
<ul> | <ul> | ||
| − | <li>Altered the electrode arrangement and size for a single well of a 96 well plate | + | <li>MEA |
| + | <ul> | ||
| + | <li>Altered the electrode arrangement and size for a single well of a 96 well plate</li> | ||
| + | <li>Predetermined specifications: Gold or TiN wires, embedded in glass </li> | ||
</ul> | </ul> | ||
| + | </li> | ||
| + | </ul> | ||
| + | |||
| + | 2 Jun 2017<br> | ||
| + | WO RG AM KP MM | ||
| + | <ul> | ||
| + | <li>Determined custom MEA design specs, particularly spacing | ||
| + | </ul> | ||
| + | |||
| + | 5 Jun 2017<br> | ||
| + | WO RG AM KP MM | ||
| + | <ul> | ||
| + | <li>Revised MEA spacing | ||
| + | </ul> | ||
| + | |||
6 Jun 2017 <br> | 6 Jun 2017 <br> | ||
| − | WO RG AM KP | + | WO RG AM KP MM |
<ul> | <ul> | ||
<li>Discussed the location of holes for media flow and a bubble trap | <li>Discussed the location of holes for media flow and a bubble trap | ||
| Line 75: | Line 93: | ||
7 Jun 2017 <br> | 7 Jun 2017 <br> | ||
| − | WO RG AM KP | + | WO RG AM KP MM |
<ul> | <ul> | ||
<li>Fleshed out design for bubble trap | <li>Fleshed out design for bubble trap | ||
| Line 81: | Line 99: | ||
</ul> | </ul> | ||
| − | 8 Jun 2017 <br> WO RG AM KP | + | 8 Jun 2017 <br> WO RG AM KP MM |
<ul> | <ul> | ||
| − | <li>Began discussion on removal of olfactant contaminated media while maintaining a perfusion system | + | <li>Began discussion on removal of olfactant contaminated media while maintaining a perfusion system via syringes |
<li>Continued clamp design-KP | <li>Continued clamp design-KP | ||
| + | <li>Requested a quote for custom MEA | ||
</ul> | </ul> | ||
| − | 9 Jun 2017 <br> WO RG AM KP | + | 9 Jun 2017 <br> WO RG AM KP MM |
| − | <ul> | + | <ul> |
<li>Confirmed the use of a peristaltic pump for media flow | <li>Confirmed the use of a peristaltic pump for media flow | ||
| + | <li>Finalized MEA design | ||
</ul> | </ul> | ||
| + | |||
| + | 12 Jun 2017<br> | ||
| + | WO RG AM KP MM PB | ||
| + | <ul> | ||
| + | <li>Contacted other MEA fabrication companies for quotes | ||
| + | </ul> | ||
| + | |||
| + | 13 Jun 2017<br> | ||
| + | WO RG AM KP MM PB | ||
| + | <ul> | ||
| + | <li>Continued search of MEA vendors | ||
| + | <li>Worked on specifics regarding flow into and out of the bioreactor/media reservoirs - decided to use luer locks | ||
| + | </ul> | ||
| + | |||
| + | 14 Jun 2017<br> | ||
| + | WO RG AM KP MM PB | ||
| + | <ul> | ||
| + | <li>Continued contacting MEA vendors | ||
| + | </ul> | ||
| + | |||
| + | 15 Jun 2017<br> | ||
| + | WO RG AM KP MM PB | ||
| + | <ul> | ||
| + | <li>Determined best luer lock connectors for tubing | ||
| + | <li>Finished printing of clamp design | ||
| + | <li>Explored photomasking vendors | ||
| + | </ul> | ||
| + | |||
| + | 16 Jun 2017<br> | ||
| + | WO RG AM KP MM PB | ||
| + | <ul> | ||
| + | <li>Determined design of media reservoirs to be conical flasks | ||
| + | <li>Explored pre-made MEA vendors | ||
| + | </ul> | ||
| + | |||
| + | |||
</html> | </html> | ||
Revision as of 16:01, 10 July 2017
USMA-West_Point
Notebook
Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.
What should this page have?
- Chronological notes of what your team is doing.
- Brief descriptions of daily important events.
- Pictures of your progress.
- Mention who participated in what task.
Inspiration
You can see what others teams have done to organize their notes:
Perfusion System with Bioreactor Design
23 May 2017WO
- Began bioreactor design based on former models to be used in a perfusion system
WO
- Used AutoDesk Inventor to design a general bioreactor
- Incorporated shape and general items such as holes for screws and cavity for neuronal culturing
- Determined location of a multielectrode array (MEA) in bioreactor design, where the neurons would be transfected and tested
WO
- Adjusted design of bioreactor design
- Began a custom design for a MEA
WO RG AM KP
- Continued MEA designing: location of electrodes, leads, contact pad spacing, materials
WO RM AM KP
- MEA
- Altered the electrode arrangement and size for a single well of a 96 well plate
- Predetermined specifications: Gold or TiN wires, embedded in glass
WO RG AM KP MM
- Determined custom MEA design specs, particularly spacing
WO RG AM KP MM
- Revised MEA spacing
WO RG AM KP MM
- Discussed the location of holes for media flow and a bubble trap
WO RG AM KP MM
- Fleshed out design for bubble trap
- Began design for clamp to keep pins on the multielectrode array (extension of bioreactor) - KP
WO RG AM KP MM
- Began discussion on removal of olfactant contaminated media while maintaining a perfusion system via syringes
- Continued clamp design-KP
- Requested a quote for custom MEA
WO RG AM KP MM
- Confirmed the use of a peristaltic pump for media flow
- Finalized MEA design
WO RG AM KP MM PB
- Contacted other MEA fabrication companies for quotes
WO RG AM KP MM PB
- Continued search of MEA vendors
- Worked on specifics regarding flow into and out of the bioreactor/media reservoirs - decided to use luer locks
WO RG AM KP MM PB
- Continued contacting MEA vendors
WO RG AM KP MM PB
- Determined best luer lock connectors for tubing
- Finished printing of clamp design
- Explored photomasking vendors
WO RG AM KP MM PB
- Determined design of media reservoirs to be conical flasks
- Explored pre-made MEA vendors
