Difference between revisions of "Team:USMA-West Point/Notebook"

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</ul>
 
</ul>
  
 +
16 Jun 2017<br>
 +
WO RG MM PB
 +
<ul>
 +
<li>Determined design of media reservoirs to be conical flasks 
 +
<li>Explored pre-made MEA vendors
 +
</ul>
  
 +
19 Jun 2017<br>
 +
WO RG MM PB
 +
<ul>
 +
<li>Ordered MEAs from Multichannel Systems
 +
<li>Researched on systems to control the peristaltic pump from the computer
 +
</ul>
 +
 +
20 Jun 2017<br>
 +
WO RG MM PB
 +
<ul>
 +
<li>Find tubing for bioreactor and pump
 +
</ul>
 +
 +
21 Jun 2017<br>
 +
WO RG MM PB
 +
<ul>
 +
<li>Designed media reservoirs in Inventor, obtaining a cubic appearance, as opposed to the original conical tube appearance
 +
</ul>
 +
 +
22 Jun 2017<br>
 +
WO RG MM PB
 +
<ul>
 +
<li>Ordered tubing
 +
</ul>
 +
 +
26 Jun 2017<br>
 +
WO RG MM PB
 +
<ul>
 +
<li>Redesigned clamp system for pins using a platform and screws system
 +
<li>Revised bioreactor design to have internal channels in one side of the bioreactor
 +
</ul>
 +
 +
27 Jun 2017<br>
 +
WO RG MM PB
 +
<ul>
 +
<li>Inserted a conical steeple for the bubble trap
 +
<li>Made minor modifications to bioreactor design
 +
</ul>
 +
 +
30 Jun 2017<br>
 +
WO RG
 +
<ul>
 +
<li>Inserted threading into media reservoirs
 +
<li>Made measurements of ordered o-rings to be fitted
 +
</ul>
 +
 +
3 Jul 2017<br>
 +
WO RG
 +
<ul>
 +
<li>Printed out models to help test fittings for the O-ring
 +
</ul>
 +
 +
5 Jul 2017<br>
 +
WO RG
 +
<ul>
 +
<li>Printed array to narrow down sizing for O-Ring
 +
<li>Modified media reservoir due to space constraints
 +
<li>Printed model MEAs to test pin setup and general fittings
 +
</ul>
 +
 +
7 Jul 2017<br>
 +
WO RG
 +
<ul>
 +
<li>Modified media reservoir for better fluid flow
 +
</ul>
 
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</html>

Revision as of 16:34, 10 July 2017

USMA-West_Point

Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

What should this page have?
  • Chronological notes of what your team is doing.
  • Brief descriptions of daily important events.
  • Pictures of your progress.
  • Mention who participated in what task.
Inspiration

You can see what others teams have done to organize their notes:

Perfusion System with Bioreactor Design

23 May 2017
WO
  • Began bioreactor design based on former models to be used in a perfusion system
25 May 2017
WO
  • Used AutoDesk Inventor to design a general bioreactor
  • Incorporated shape and general items such as holes for screws and cavity for neuronal culturing
  • Determined location of a multielectrode array (MEA) in bioreactor design, where the neurons would be transfected and tested
30 May 2017
WO
  • Adjusted design of bioreactor design
  • Began a custom design for a MEA
31 May 2017
WO RG AM KP
  • Continued MEA designing: location of electrodes, leads, contact pad spacing, materials
1 Jun 2017
WO RM AM KP
  • MEA
    • Altered the electrode arrangement and size for a single well of a 96 well plate
    • Predetermined specifications: Gold or TiN wires, embedded in glass
2 Jun 2017
WO RG AM KP MM
  • Determined custom MEA design specs, particularly spacing
5 Jun 2017
WO RG AM KP MM
  • Revised MEA spacing
6 Jun 2017
WO RG AM KP MM
  • Discussed the location of holes for media flow and a bubble trap
7 Jun 2017
WO RG AM KP MM
  • Fleshed out design for bubble trap
  • Began design for clamp to keep pins on the multielectrode array (extension of bioreactor) - KP
8 Jun 2017
WO RG AM KP MM
  • Began discussion on removal of olfactant contaminated media while maintaining a perfusion system via syringes
  • Continued clamp design-KP
  • Requested a quote for custom MEA
9 Jun 2017
WO RG AM KP MM
  • Confirmed the use of a peristaltic pump for media flow
  • Finalized MEA design
12 Jun 2017
WO RG AM KP MM PB
  • Contacted other MEA fabrication companies for quotes
13 Jun 2017
WO RG AM KP MM PB
  • Continued search of MEA vendors
  • Worked on specifics regarding flow into and out of the bioreactor/media reservoirs - decided to use luer locks
14 Jun 2017
WO RG AM KP MM PB
  • Continued contacting MEA vendors
15 Jun 2017
WO RG AM KP MM PB
  • Determined best luer lock connectors for tubing
  • Finished printing of clamp design
  • Explored photomasking vendors
16 Jun 2017
WO RG AM KP MM PB
  • Determined design of media reservoirs to be conical flasks
  • Explored pre-made MEA vendors
16 Jun 2017
WO RG MM PB
  • Determined design of media reservoirs to be conical flasks
  • Explored pre-made MEA vendors
19 Jun 2017
WO RG MM PB
  • Ordered MEAs from Multichannel Systems
  • Researched on systems to control the peristaltic pump from the computer
20 Jun 2017
WO RG MM PB
  • Find tubing for bioreactor and pump
21 Jun 2017
WO RG MM PB
  • Designed media reservoirs in Inventor, obtaining a cubic appearance, as opposed to the original conical tube appearance
22 Jun 2017
WO RG MM PB
  • Ordered tubing
26 Jun 2017
WO RG MM PB
  • Redesigned clamp system for pins using a platform and screws system
  • Revised bioreactor design to have internal channels in one side of the bioreactor
27 Jun 2017
WO RG MM PB
  • Inserted a conical steeple for the bubble trap
  • Made minor modifications to bioreactor design
30 Jun 2017
WO RG
  • Inserted threading into media reservoirs
  • Made measurements of ordered o-rings to be fitted
3 Jul 2017
WO RG
  • Printed out models to help test fittings for the O-ring
5 Jul 2017
WO RG
  • Printed array to narrow down sizing for O-Ring
  • Modified media reservoir due to space constraints
  • Printed model MEAs to test pin setup and general fittings
7 Jul 2017
WO RG
  • Modified media reservoir for better fluid flow