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<h3>Neurons</h3> | <h3>Neurons</h3> | ||
− | < | + | 9 Jun 2017 <br> WO RG AM KP MM |
+ | <ul> | ||
+ | <li>Received HT-22 Immortalized Mouse Hippocampal Cell Line and related materials from West Point | ||
+ | </ul> | ||
+ | |||
+ | 13 Jun 2017 <br> WO RG AM KP MM PB | ||
+ | <ul> | ||
+ | <li>Unfroze neurons | ||
+ | <li>Plated neurons for amplification | ||
+ | </ul> | ||
+ | |||
+ | 15 Jun 2017 <br> WO RG AM KP MM PB | ||
+ | <ul> | ||
+ | <li>Change media | ||
+ | </ul> | ||
+ | |||
+ | 17 Jun 2017 <br> JG | ||
+ | <ul> | ||
+ | <li>Changed media | ||
+ | </ul> | ||
+ | |||
+ | 19 Jun 2017 <br> WO RG MM PB | ||
+ | <ul> | ||
+ | <li>Split cells - Passage 1 from receipt of neurons | ||
+ | </ul> | ||
+ | |||
+ | 21 Jun 2017 <br> WO RG MM PB | ||
+ | <ul> | ||
+ | <li>Changed media | ||
+ | <li>Froze excess cells | ||
+ | </ul> | ||
+ | |||
+ | 23 Jun 2017 <br> JG | ||
+ | <ul> | ||
+ | <li>Changed media | ||
+ | </ul> | ||
+ | |||
+ | 27 Jun 2017 <br> WO RG | ||
+ | <ul> | ||
+ | <li>Made and changed media | ||
+ | </ul> | ||
+ | |||
+ | 30 Jun 2017 <br> WO RG | ||
+ | <ul> | ||
+ | <li>Thawed and plated cells - Passage 2 | ||
+ | </ul> | ||
+ | |||
+ | 5 Jul 2017 <br> WO RG | ||
+ | <ul> | ||
+ | <li>Split cells - Passage 3 | ||
+ | </ul> | ||
+ | |||
+ | 7 Jul 2017 <br> WO RG | ||
+ | <ul> | ||
+ | <li>Changed media | ||
+ | </ul> | ||
+ | |||
+ | 10 Jul 2017 <br> WO RG | ||
+ | <ul> | ||
+ | <li>Split cells - Passage 4 | ||
+ | </ul> | ||
+ | |||
+ | name="Perfusion System with Bioreactor Design"></a> <br> | ||
<h3>Perfusion System with Bioreactor Design</h3> | <h3>Perfusion System with Bioreactor Design</h3> | ||
Revision as of 14:37, 11 July 2017
USMA-West_Point
Notebook
Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.
What should this page have?
- Chronological notes of what your team is doing.
- Brief descriptions of daily important events.
- Pictures of your progress.
- Mention who participated in what task.
Inspiration
You can see what others teams have done to organize their notes:
Perfusion System with Bioreactor Design
Programming and Electronics (Bioreactor focus)
Neurons
9 Jun 2017WO RG AM KP MM
- Received HT-22 Immortalized Mouse Hippocampal Cell Line and related materials from West Point
WO RG AM KP MM PB
- Unfroze neurons
- Plated neurons for amplification
WO RG AM KP MM PB
- Change media
JG
- Changed media
WO RG MM PB
- Split cells - Passage 1 from receipt of neurons
WO RG MM PB
- Changed media
- Froze excess cells
JG
- Changed media
WO RG
- Made and changed media
WO RG
- Thawed and plated cells - Passage 2
WO RG
- Split cells - Passage 3
WO RG
- Changed media
WO RG
- Split cells - Passage 4
Perfusion System with Bioreactor Design
23 May 2017WO
- Began bioreactor design based on former models to be used in a perfusion system
WO
- Used AutoDesk Inventor to design a general bioreactor
- Incorporated shape and general items such as holes for screws and cavity for neuronal culturing
- Determined location of a multielectrode array (MEA) in bioreactor design, where the neurons would be transfected and tested
WO
- Adjusted design of bioreactor design
- Began a custom design for a MEA
WO RG AM KP
- Continued MEA designing: location of electrodes, leads, contact pad spacing, materials
WO RM AM KP
- MEA
- Altered the electrode arrangement and size for a single well of a 96 well plate
- Predetermined specifications: Gold or TiN wires, embedded in glass
WO RG AM KP MM
- Determined custom MEA design specs, particularly spacing
WO RG AM KP MM
- Revised MEA spacing
WO RG AM KP MM
- Discussed the location of holes for media flow and a bubble trap
WO RG AM KP MM
- Fleshed out design for bubble trap
- Began design for clamp to keep pins on the multielectrode array (extension of bioreactor) - KP
WO RG AM KP MM
- Began discussion on removal of olfactant contaminated media while maintaining a perfusion system via syringes
- Continued clamp design-KP
- Requested a quote for custom MEA
WO RG AM KP MM
- Confirmed the use of a peristaltic pump for media flow
- Finalized MEA design
WO RG AM KP MM PB
- Contacted other MEA fabrication companies for quotes
WO RG AM KP MM PB
- Continued search of MEA vendors
- Worked on specifics regarding flow into and out of the bioreactor/media reservoirs - decided to use luer locks
WO RG AM KP MM PB
- Continued contacting MEA vendors
WO RG AM KP MM PB
- Determined best luer lock connectors for tubing
- Finished printing of clamp design
- Explored photomasking vendors
WO RG AM KP MM PB
- Determined design of media reservoirs to be conical flasks
- Explored pre-made MEA vendors
WO RG MM PB
- Determined design of media reservoirs to be conical flasks
- Explored pre-made MEA vendors
WO RG MM PB
- Ordered MEAs from Multichannel Systems
- Researched on systems to control the peristaltic pump from the computer
WO RG MM PB
- Find tubing for bioreactor and pump
WO RG MM PB
- Designed media reservoirs in Inventor, obtaining a cubic appearance, as opposed to the original conical tube appearance
WO RG MM PB
- Ordered tubing
WO RG MM PB
- Redesigned clamp system for pins using a platform and screws system
- Revised bioreactor design to have internal channels in one side of the bioreactor
WO RG MM PB
- Inserted a conical steeple for the bubble trap
- Made minor modifications to bioreactor design
WO RG
- Inserted threading into media reservoirs
- Made measurements of ordered o-rings to be fitted
WO RG
- Printed out models to help test fittings for the O-ring
WO RG
- Printed array to narrow down sizing for O-Ring
- Modified media reservoir due to space constraints
- Printed model MEAs to test pin setup and general fittings
WO RG
- Modified media reservoir for better fluid flow
WO RG
- Printed first part of bioreactor