Team:USMA-West Point/Notebook

USMA-West_Point

Notebook

Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.

What should this page have?
  • Chronological notes of what your team is doing.
  • Brief descriptions of daily important events.
  • Pictures of your progress.
  • Mention who participated in what task.
Inspiration

You can see what others teams have done to organize their notes:

Neurons
Perfusion System with Bioreactor Design
Programming and Electronics (Bioreactor focus)

Neurons

9 Jun 2017
WO RG AM KP MM
  • Received HT-22 Immortalized Mouse Hippocampal Cell Line and related materials from West Point
13 Jun 2017
WO RG AM KP MM PB
  • Unfroze neurons
  • Plated neurons for amplification
15 Jun 2017
WO RG AM KP MM PB
  • Change media
17 Jun 2017
JG
  • Changed media
19 Jun 2017
WO RG MM PB
  • Split cells - Passage 1 from receipt of neurons
21 Jun 2017
WO RG MM PB
  • Changed media
  • Froze excess cells
23 Jun 2017
JG
  • Changed media
27 Jun 2017
WO RG
  • Made and changed media
30 Jun 2017
WO RG
  • Thawed and plated cells - Passage 2
5 Jul 2017
WO RG
  • Split cells - Passage 3
7 Jul 2017
WO RG
  • Changed media
10 Jul 2017
WO RG
  • Split cells - Passage 4
13 Jul 2017
WO RG
  • Changed media
17 Jul 2017
JG
  • Cells were killed

Perfusion System with Bioreactor Design

23 May 2017
WO
  • Began bioreactor design based on former models to be used in a perfusion system
25 May 2017
WO
  • Used AutoDesk Inventor to design a general bioreactor
  • Incorporated shape and general items such as holes for screws and cavity for neuronal culturing
  • Determined location of a multielectrode array (MEA) in bioreactor design, where the neurons would be transfected and tested
30 May 2017
WO
  • Adjusted design of bioreactor design
  • Began a custom design for a MEA
31 May 2017
WO RG AM KP
  • Continued MEA designing: location of electrodes, leads, contact pad spacing, materials
1 Jun 2017
WO RM AM KP
  • MEA
    • Altered the electrode arrangement and size for a single well of a 96 well plate
    • Predetermined specifications: Gold or TiN wires, embedded in glass
2 Jun 2017
WO RG AM KP MM
  • Determined custom MEA design specs, particularly spacing
5 Jun 2017
WO RG AM KP MM
  • Revised MEA spacing
6 Jun 2017
WO RG AM KP MM
  • Discussed the location of holes for media flow and a bubble trap
7 Jun 2017
WO RG AM KP MM
  • Fleshed out design for bubble trap
  • Began design for clamp to keep pins on the multielectrode array (extension of bioreactor) - KP
8 Jun 2017
WO RG AM KP MM
  • Began discussion on removal of olfactant contaminated media while maintaining a perfusion system via syringes
  • Continued clamp design-KP
  • Requested a quote for custom MEA
9 Jun 2017
WO RG AM KP MM
  • Confirmed the use of a peristaltic pump for media flow
  • Finalized MEA design
12 Jun 2017
WO RG AM KP MM PB
  • Contacted other MEA fabrication companies for quotes
13 Jun 2017
WO RG AM KP MM PB
  • Continued search of MEA vendors
  • Worked on specifics regarding flow into and out of the bioreactor/media reservoirs - decided to use luer locks
14 Jun 2017
WO RG AM KP MM PB
  • Continued contacting MEA vendors
15 Jun 2017
WO RG AM KP MM PB
  • Determined best luer lock connectors for tubing
  • Finished printing of clamp design
  • Explored photomasking vendors
16 Jun 2017
WO RG AM KP MM PB
  • Determined design of media reservoirs to be conical flasks
  • Explored pre-made MEA vendors
16 Jun 2017
WO RG MM PB
  • Determined design of media reservoirs to be conical flasks
  • Explored pre-made MEA vendors
19 Jun 2017
WO RG MM PB
  • Ordered MEAs from Multichannel Systems
  • Researched on systems to control the peristaltic pump from the computer
20 Jun 2017
WO RG MM PB
  • Find tubing for bioreactor and pump
21 Jun 2017
WO RG MM PB
  • Designed media reservoirs in Inventor, obtaining a cubic appearance, as opposed to the original conical tube appearance
22 Jun 2017
WO RG MM PB
  • Ordered tubing
26 Jun 2017
WO RG MM PB
  • Redesigned clamp system for pins using a platform and screws system
  • Revised bioreactor design to have internal channels in one side of the bioreactor
27 Jun 2017
WO RG MM PB
  • Inserted a conical steeple for the bubble trap
  • Made minor modifications to bioreactor design
30 Jun 2017
WO RG
  • Inserted threading into media reservoirs
  • Made measurements of ordered o-rings to be fitted
3 Jul 2017
WO RG
  • Printed out models to help test fittings for the O-ring
5 Jul 2017
WO RG
  • Printed array to narrow down sizing for O-Ring
  • Modified media reservoir due to space constraints
  • Printed model MEAs to test pin setup and general fittings
7 Jul 2017
WO RG
  • Modified media reservoir for better fluid flow
10 Jul 2017
WO RG
  • Printed first part of bioreactor
12 Jul 2017
WO RG
  • Adjusted dimensions and reprinted first part of bioreactor
13 Jul 2017
WO RG
  • Adjusted dimensions and printed a third version of the first part of bioreactor
14 Jul 2017
WO RG
  • Printed array to determine necessary dimensions to fit luer locks
17 Jul 2017
WO RG
  • Applied dimension changes to bioreactor design and reprinted the first part of bioreactor
18 Jul 2017
WO RG
  • Determined screw size and lengths needed to apply appropriate pressure on pins
19 Jul 2017
WO RG
  • Printed out top and bottom of bioreactor and clamp
  • Perforated MEA Bioreactor modified
  • Verified operation of the pump
  • Tested calibration of pump

Programming and Electronics (Bioreactor focus)

23 May 2017
WO
  • Introduced to labview - use to take voltage readings from electrodes
  • Determined to set peak threshold to +/- 2 SD of base activity
25 May 2017
WO
  • Built time-averaging method to determine frequency and amplitude of peaks
  • Generated preliminary statistics for data
  • Built code to write to a file
30 May 2017
WO
  • Inserted method to adjust thresholds before data collection
29 Jun 2017
WO
  • Adjusted labview program to read in data from electrodes using Intan codes (as opposed to theoretical inputs)
  • Finished labview program - can select filename/type
12 Jun 2017
WO
  • Implemented impedance program from Intan to determine the presence of cells on electrodes
13 Jul 2017
WO RG
  • Soldered pins to electrode adapter boards
19 Jul 2017
WO RG
  • Tested program that ran impedance tests and voltage readings
  • Soldered more electrode adapters