Difference between revisions of "Team:USMA-West Point/Results"

(Prototype team page)
 
Line 1: Line 1:
 
{{USMA-West_Point}}
 
{{USMA-West_Point}}
−
<html>
 
  
 +
<!DOCTYPE html>
 +
<html xmlns="http://www.w3.org/1999/xhtml">
 +
<head><meta name='robots' content='noindex, nofollow'>
 +
<title>
 +
Results - iGem 01</title>
 +
<!-- META -->
 +
<meta charset="UTF-8">
 +
<meta name="viewport" content="width=device-width, maximum-scale=1"/>
 +
 +
 +
 +
 +
<script src="https://www.youtube.com/iframe_api"></script>
 +
<!-- CSS -->
 +
<link rel="stylesheet" href="//www.imcreator.com/css/common.css?v=1.4.1f">
 +
<link rel="stylesheet" type="text/css" href="//www.imcreator.com/all_css.css?v=1.4.1f" />
  
−
<div class="column full_size" >
+
 +
 +
<link rel="icon" type="image/png" href="https://lh3.googleusercontent.com/dd_e0xA19up9208Tv6odcjHEw6z4cKAA6fTgjZ9ynkKoSnr5R4vFxI7gZp6pnZH1Vi5T5f-fphjFsrLG=s30" />
 +
<link rel="apple-touch-icon" href="https://lh3.googleusercontent.com/dd_e0xA19up9208Tv6odcjHEw6z4cKAA6fTgjZ9ynkKoSnr5R4vFxI7gZp6pnZH1Vi5T5f-fphjFsrLG=s30" />
 +
 +
 +
 +
<link rel="stylesheet" type="text/css" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css" />
 +
 +
<link id="vbid-5734f2ef-jztngyge-STRIPE_DATA" rel="stylesheet" type="text/css"  href="//www.imcreator.com/stripe_data_css?page_id=vbid-5734f2ef-jztngyge-STRIPE_DATA&v=1.4.1f">
 +
 +
<link id="vbid-7d38c6a1-ojqdlra3-STRIPE_DATA" rel="stylesheet" type="text/css"  href="//www.imcreator.com/stripe_data_css?page_id=vbid-7d38c6a1-ojqdlra3-STRIPE_DATA&v=1.4.1f">
 +
 +
<link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/fonts.css?v=1.4.1f" />
 +
<link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/effects.css?v=1.4.1f" />
 +
<link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/lightbox.css?v=1.4.1f">
 +
 +
<link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/previewhelper.css?v=1.4.1f">
 +
 +
<link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/spimeview.css?v=1.4.1f">
 +
 +
<link id="vbid-5734f2ef-jztngyge-STATIC_STYLE" rel="stylesheet" type="text/css" href="//www.imcreator.com/static_style?v=1.4.1f&vbid=vbid-5734f2ef-jztngyge&caller=static">
 +
 +
 +
<!-- SCRIPT -->
 +
<script src="https://code.jquery.com/jquery-2.x-git.min.js" type="text/javascript"></script>
 +
<script src="https://maps.googleapis.com/maps/api/js?key=AIzaSyByWRZWFbQRxJM7SiNz2NURPDPJPqTtBQQ" type="text/javascript"></script>
 +
 +
<script src="//www.imcreator.com/js/xprs_helper.js?v=1.4.1f" type="text/javascript"></script>
 +
 +
<script src="https://imos004-dot-im--os.appspot.com/js/imos.js?v=1.4.1f" type="text/javascript"></script>
  
−
<h1>Results</h1>
+
<script type="text/javascript" src="//www.imcreator.com/all_js.js?v=1.4.1f"></script>
 +
<script src="//www.imcreator.com/js/lib/touchswipe/jquery.mobile.custom.min.js"></script>
 +
<script type="text/javascript">IMOS.pageView();</script>
 +
 +
 +
 +
 +
<script src="//www.imcreator.com/js/preview_helper.js?v=1.4.1f"  type="text/javascript"></script>
 +
 +
 +
<!-- User analytics -->
  
−
<p>Here you can describe the results of your project and your future plans. </p>
 
  
−
<h5>What should this page contain?</h5>
+
<!-- Serving analytics -->
−
<ul>
+
<script>
−
<li> Clearly and objectively describe the results of your work.</li>
+
var currentDomain =  window.location.host;
−
<li> Future plans for the project. </li>
+
if (currentDomain != "localhost:7000"){
−
<li> Considerations for replicating the experiments. </li>
+
var _gaq = _gaq || [];
−
</ul>
+
_gaq.push(['_setAccount', 'UA-58695071-1']);
 +
_gaq.push(['_setDomainName', currentDomain]);
 +
_gaq.push(['_setAllowLinker', true]);
 +
_gaq.push(['_trackPageview']);
 +
}
 +
</script>
 +
 +
<!-- Label config-->
 +
<script>
 +
 +
var LABEL_CONFIG = {};
 +
 +
</script></head>
  
−
<h5>You should also describe what your results mean: </h5>
+
<body class="fast-animated-bg pattern-bg " data-ecommerce-solution="NONE" data-root-id="vbid-7d38c6a1-ojqdlra3" data-root-style-id="style-7d38c6a1-d2bgnenh" data-default-currency="USD" data-osid="osid--ec2fa157-ff75c294"  data-app-version="1.4.1f" data-caller="static"  data-ecommerce-dashboard="https://dashboard.shoprocket.co" data-static-server="//www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com">
 +
 +
<div id="xprs" data-website-name="iGem 01" class="xprs-holder" >
 +
<div class="main-page "  >
 +
<div id="content">
 +
<div  id="vbid-5734f2ef-jztngyge"  class="master container style-5734f2ef-9z1ag17a content stripes  website-style " data-itemtype="folder" data-creator="" data-itemname="Results"  data-itemslug="results" data-itemstyleid="style-5734f2ef-9z1ag17a" data-margintop="" data-arranger="stripes" data-layout="multi" data-vbid="vbid-5734f2ef-jztngyge" data-preset-type-id="ITEMS" data-preview-style="style-5734f2ef-9z1ag17a" data-style="style-7f715-fyu5oogf7j" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="stripes"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="false"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT=""
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
 +
<!-- MENUS START -->
 +
<div  id="vbid-7d38c6a1-8otprari"  class="master item-box  header-box style-7d38c6a1-8jo8o4ao injected        " data-holder-type="header"" data-holder-type="header"  data-child-type="ORIGIN"  data-styleid="style-7d38c6a1-8jo8o4ao" data-preview-styleid='style-7d38c6a1-8jo8o4ao' data-preset-type-id="MENUS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="header-wrapper item-wrapper menus-wrapper ">
 +
 +
<div class="item-content leaf menu_layout header content" data-preview-style="style-7d38c6a1-8jo8o4ao" data-style="style-7f715-scjjarqgxu"  data-behavior-type="NOTHING" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-7d38c6a1-8otprari" data-bgimg="">
 +
<div class="preview-content-wrapper">
 +
<div class="preview-content-holder">
 +
<div class="left-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<div class="logo-holder">
 +
<!-- ICON TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
  
−
<ul>
+
</div>
−
<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
+
<div class="helper-div">
−
<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
+
<div class="item-details menu">
−
<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
+
<!-- TITLE TEMPLATE -->
−
</ul>
+
 +
<div class="preview-title-holder removable-parent">
 +
 +
<a href="/free/amitro02/igem-01" data-link-type="EXISTING"  target="_self" >
 +
 +
<h2 id="vbid-7d38c6a1-8ye3bio7" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >usMA-WEST POINT<br></h2>
 +
</a>
 +
</div>
 +
<br />
  
 +
<!-- SUBTITLE TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
</div>
 +
<div class="right-div">
 +
<div class="benet" style="min-height:inherit;"></div>
 +
<!-- LINKS TEMPLATE -->
 +
<div class="preview-item-links ">
 +
<ul class="preview-links-wrapper">
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="vbid-7d38c6a1-0vahew5d"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Home</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
 +
 +
 +
<li>
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/vbid-f02482b7-t3dtwkay" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="c4532978"  class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Team</span>
 +
</a>
 +
<ul class="submenu">
 +
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/team" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="e14b181a" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Students, Advisors, PIs, and Instructors</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/collaboration" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="facc214a" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Collaboration</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
 +
</ul>
 +
</li class="removable-parent">
 +
 +
 +
 +
<li>
 +
 +
<span id="element-4cd1f662a476dc8"  class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Project</span>
 +
 +
<ul class="submenu">
 +
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/project" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="38c7042b" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Project</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/description" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="42d444bb" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Description</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/design" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="59bf92c2" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Design</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/experiments" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="487700ca" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Experiments</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/notebook" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="ab39f1bd" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Notebook</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/interlab-study" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="21fa8523" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >InterLab Study</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/results" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="cff7eeb1" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Results</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/demonstrate" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="6157824b" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Demonstrate</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/attribution" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="a0b12bde" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Attribution</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<!-- [] -->
 +
</ul>
 +
</li>
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/deliverables" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="09c40e1e"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Deliverables</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
 +
 +
 +
<li>
 +
 +
<span id="element-78132adefe1a4d4"  class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Parts</span>
 +
 +
<ul class="submenu">
 +
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/basic-parts" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="fa2fa5ec" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Basic Parts</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/parts-collection" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="37cd96f3" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Parts Collection</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<!-- [] -->
 +
</ul>
 +
</li>
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/other" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="99ad6039"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span>
 +
</a>
 +
 +
</li>
 +
 +
 +
 +
 +
 +
 +
<li>
 +
 +
<span id="element-b165365db185e95"  class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Human Practices </span>
 +
 +
<ul class="submenu">
 +
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/human-practices" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="61c8e27f" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Integrated and Gold</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/public-engagement" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="f9e986dd" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Public Engagement</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
 +
</ul>
 +
</li class="removable-parent">
 +
 +
 +
 +
<li>
 +
 +
<span id="element-905a6c68c42aebb"  class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Awards</span>
 +
 +
<ul class="submenu">
 +
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/entreneurship" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="ca6cd864" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Entreneurship</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/extra" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="bc7909bf" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Applied design</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
 +
 +
<li class="removable-parent">
 +
 +
<a class="removable-parent" href="/free/amitro02/igem-01/hardware" data-link-type="EXISTING"  target="_self" >
 +
 +
<span id="c5142c05" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Hardware</span>
 +
</a>
 +
 +
</li>
 +
 +
</li>
 +
 +
 +
<!-- [1] -->
 +
</ul>
 +
</li>
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</ul>
 +
</div>
 +
 +
 +
<button class="hamburger links-menu-btn  small" type="button">
 +
  <span class="hamburger-box">
 +
  <span class="hamburger-inner"></span>
 +
  </span>
 +
</button>
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="menu"
 +
data-MENU_OVERLAY="relative"
 +
data-MENU_SCROLL="true"
 +
data-ALWAYS_MINIFIED="false"
 +
data-MENU_POSITION="top"
 +
data-MENU_ALIGN="left"
 +
data-BACKGROUND_COLOR="rgb(201, 151, 0)"
 +
></div>
 
</div>
 
</div>
 +
 +
</div>
 +
</div>
 +
<!-- MENUS END -->
 +
 +
 +
 +
 +
<!-- PROMO START -->
 +
<div  id="vbid-5734f2ef-saamffpj"  class="master item-box  gallery-box style-5734f2ef-tyqrxh4f    button-effects btn_hover3  items-enter-effects item_enter2    " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-5734f2ef-tyqrxh4f" data-preview-styleid='style-5734f2ef-tyqrxh4f' data-preset-type-id="PROMO">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper promo-wrapper ">
 +
 +
<div  class="sub container style-5734f2ef-tyqrxh4f content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-5734f2ef-tyqrxh4f" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-5734f2ef-saamffpj" data-preset-type-id="PROMO" data-preview-style="style-5734f2ef-tyqrxh4f" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-5734f2ef-t7bxpo6s"  class="sub item-box  page-box style-5734f2ef-tyqrxh4f          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-5734f2ef-tyqrxh4f" data-preview-styleid='style-5734f2ef-tyqrxh4f' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-5734f2ef-t7bxpo6s" data-preview-style="style-5734f2ef-tyqrxh4f" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="814" data-orig-thumb-width="3264" data-vbid="vbid-5734f2ef-t7bxpo6s" data-bgimg="https://lh3.googleusercontent.com/Y_fHvvRrbYHtoqH1YU5OR8UrnViHDlVEXPdZ55NnjysVmgKGC13On5nKWAGPcBTgedmmNUL0dZY9MJ8NnA">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-5734f2ef-hibgobtn-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
−
<div class="clear"></div>
+
<div id="vbid-5734f2ef-hibgobtn" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/Y_fHvvRrbYHtoqH1YU5OR8UrnViHDlVEXPdZ55NnjysVmgKGC13On5nKWAGPcBTgedmmNUL0dZY9MJ8NnA=s300);"  data-orig-width="3264" data-orig-height="814" >
 +
<!-- VIDEO TEMPLATE -->
 +
  
−
<div class="column half_size" >
+
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
  
  
−
<h5> Project Achievements </h5>
+
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
  
−
<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
+
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
  
−
<ul>
+
</div>
−
<li>A list of linked bullet points of the successful results during your project</li>
+
−
<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
+
</div>
−
</ul>
+
  
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h1 id="vbid-5734f2ef-4t0dorlc" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Results<br></h1>
 +
 
</div>
 
</div>
 +
<br class="lower-line-break" />
  
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-5734f2ef-gehbufaw" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="font_7" style="text-align: center; line-height: 1.5em;">Here we present results from three different components: 1) Plasmid design and development, 2) Animal Cell Nucleofection, and 3) Device design.<br></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
  
−
<div class="column half_size" >
+
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- PROMO END -->
 +
 +
 +
 +
 +
<!-- PROJECTS START -->
 +
<div  id="vbid-6862ab34-aatxbqmn"  class="master item-box  gallery-box style-6862ab34-fsvthdyw          " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="NATIVE_ORDER"  data-styleid="style-6862ab34-fsvthdyw" data-preview-styleid='style-6862ab34-fsvthdyw' data-preset-type-id="PROJECTS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper projects-wrapper ">
 +
 +
<div  class="sub container style-6862ab34-fsvthdyw content matrix  " data-itemtype="folder" data-creator="" data-itemname="LOADING"  data-itemslug="loading" data-itemstyleid="style-6862ab34-fsvthdyw" data-margintop="" data-arranger="matrix" data-layout="multi" data-vbid="vbid-6862ab34-aatxbqmn" data-preset-type-id="PROJECTS" data-preview-style="style-6862ab34-fsvthdyw" data-style="style-8ec4c-t1avbp6mmi" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="matrix"
 +
data-ARRANGER_COLS="3"
 +
data-ARRANGER_ITEM_MAX_WIDTH="1000"
 +
data-ARRANGER_ITEM_MIN_WIDTH="219.1"
 +
data-ARRANGER_ITEM_RATIO="1.0"
 +
data-ARRANGER_ITEM_SPACING="15"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="false"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT=""
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<!-- STRIPE HEADER -->
 +
<div class="stripe-header-wrapper">
 +
<div  id="vbid-6862ab34-dz8jy3lh"  class="stripe-header sub item-box page-box style-6862ab34-fsvthdyw      " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-6862ab34-fsvthdyw" data-preview-styleid='style-6862ab34-fsvthdyw' data-preset-type-id="UNRESOLVED">
 +
<div class="page-wrapper item-wrapper ">
 +
<div class="item-content leaf blocks_layout page content" data-self="vbid-6862ab34-dz8jy3lh" data-preview-style="style-6862ab34-fsvthdyw" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-6862ab34-dz8jy3lh" data-bgimg="">
 +
<div class="helper-div middle" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="text-side ">
 +
<div class="vertical-aligner">
 +
<div class="item-details blocks-preview-content-wrapper  blocks shrinker-parent" style="position:relative;">
 +
<div class="blocks-preview-content-holder shrinker-content">
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="blocks-preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-6862ab34-k86zhhid" class="preview-element blocks-preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="BLOCKS_PREVIEW_TITLE" >Plasmid Design and Development</h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
  
−
<h5>Inspiration</h5>
 
−
<p>See how other teams presented their results.</p>
 
−
<ul>
 
−
<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
−
</ul>
 
  
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="blocks-preview-body-holder removable-parent order-handle">
 +
 +
<div id="element-8a604c4bc121774" class="preview-element blocks-preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="BLOCKS_PREVIEW_BODY">
 +
<p><span style="color: rgb(0, 0, 0);">Plasmid design and development is a key component of the eNose project. Since odorant receptor protein function was tested in a mammalian cell line, the plasmids used to drive expression of these proteins were specifically designed for mammalian cells. For example, the odorant receptor proteins OR10G4 and OR2W1 were expressed using the pCMV6 and pCI plasmids, respectively, and both contain a cytomegalovirus (CMV) promoter region for high gene expression levels in mammalian cells.</span></p>
 +
</div>
 +
 
</div>
 
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="blocks"></div>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-6862ab34-pl59vaem"  class="sub item-box  page-box style-6862ab34-fsvthdyw          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-6862ab34-fsvthdyw" data-preview-styleid='style-6862ab34-fsvthdyw' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-6862ab34-pl59vaem" data-preview-style="style-6862ab34-fsvthdyw" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="1018" data-orig-thumb-width="1086" data-vbid="vbid-6862ab34-pl59vaem" data-bgimg="https://lh3.googleusercontent.com/GmUVauvSM4q1F8T6RGDs41OYsq9bN0V0gNH92lCbNYmijgfqG28kjTgGhwnwqUaMHduQ1e8mgPd4k2AQGA">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div bottom-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-6862ab34-oxn9qu7b-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
  
 +
<div  id="vbid-6862ab34-oxn9qu7b" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/GmUVauvSM4q1F8T6RGDs41OYsq9bN0V0gNH92lCbNYmijgfqG28kjTgGhwnwqUaMHduQ1e8mgPd4k2AQGA=s300);"  data-orig-width="1086" data-orig-height="1018" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 +
 +
<div class="preview-form order-handle">
 +
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-6862ab34-fhxskqns"  class="sub item-box  page-box style-6862ab34-fsvthdyw          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-6862ab34-fsvthdyw" data-preview-styleid='style-6862ab34-fsvthdyw' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-6862ab34-fhxskqns" data-preview-style="style-6862ab34-fsvthdyw" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="996" data-orig-thumb-width="1049" data-vbid="vbid-6862ab34-fhxskqns" data-bgimg="https://lh3.googleusercontent.com/2wJ8oDa_IKchzZ_15nHnTy2LGDQbWujeavpleuqnx8yqsiQN2nLDCqFzVh-rEikelEObck4g5gSLLh3v2ew">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div bottom-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-6862ab34-trna2tpa-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-6862ab34-trna2tpa" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/2wJ8oDa_IKchzZ_15nHnTy2LGDQbWujeavpleuqnx8yqsiQN2nLDCqFzVh-rEikelEObck4g5gSLLh3v2ew=s300);"  data-orig-width="1049" data-orig-height="996" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 +
 +
<div class="preview-form order-handle">
 +
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-6862ab34-pdgrzays"  class="sub item-box  page-box style-6862ab34-fsvthdyw          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-6862ab34-fsvthdyw" data-preview-styleid='style-6862ab34-fsvthdyw' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-6862ab34-pdgrzays" data-preview-style="style-6862ab34-fsvthdyw" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="1191" data-orig-thumb-width="855" data-vbid="vbid-6862ab34-pdgrzays" data-bgimg="https://lh3.googleusercontent.com/oWxTg5xYHS6yJukTKELa5dKxAMZg_sN9VdzA2seb7KYMjw7r_IE5nkH-xMk-hZg1ydVYm7XUNsuCnDP6xkE">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div bottom-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-6862ab34-tubzmf7g-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-6862ab34-tubzmf7g" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/oWxTg5xYHS6yJukTKELa5dKxAMZg_sN9VdzA2seb7KYMjw7r_IE5nkH-xMk-hZg1ydVYm7XUNsuCnDP6xkE=s300);"  data-orig-width="855" data-orig-height="1191" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 +
 +
<div class="preview-form order-handle">
 +
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- PROJECTS END -->
 +
 +
 +
 +
 +
<!-- PROJECTS START -->
 +
<div  id="vbid-2a27a910-ffov8ogy"  class="master item-box  gallery-box style-2a27a910-2asblupt          " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="NATIVE_ORDER"  data-styleid="style-2a27a910-2asblupt" data-preview-styleid='style-2a27a910-2asblupt' data-preset-type-id="PROJECTS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper projects-wrapper ">
 +
 +
<div  class="sub container style-2a27a910-2asblupt content matrix  " data-itemtype="folder" data-creator="" data-itemname="LOADING"  data-itemslug="loading" data-itemstyleid="style-2a27a910-2asblupt" data-margintop="" data-arranger="matrix" data-layout="multi" data-vbid="vbid-2a27a910-ffov8ogy" data-preset-type-id="PROJECTS" data-preview-style="style-2a27a910-2asblupt" data-style="style-8ec4c-t1avbp6mmi" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="matrix"
 +
data-ARRANGER_COLS="3"
 +
data-ARRANGER_ITEM_MAX_WIDTH="1000"
 +
data-ARRANGER_ITEM_MIN_WIDTH="338.8"
 +
data-ARRANGER_ITEM_RATIO="1.0"
 +
data-ARRANGER_ITEM_SPACING="15"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="false"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT=""
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<!-- STRIPE HEADER -->
 +
<div class="stripe-header-wrapper">
 +
<div  id="vbid-2a27a910-gg22i4be"  class="stripe-header sub item-box page-box style-2a27a910-2asblupt      " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-2a27a910-2asblupt" data-preview-styleid='style-2a27a910-2asblupt' data-preset-type-id="UNRESOLVED">
 +
<div class="page-wrapper item-wrapper ">
 +
<div class="item-content leaf blocks_layout page content" data-self="vbid-2a27a910-gg22i4be" data-preview-style="style-2a27a910-2asblupt" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-2a27a910-gg22i4be" data-bgimg="">
 +
<div class="helper-div middle" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="text-side ">
 +
<div class="vertical-aligner">
 +
<div class="item-details blocks-preview-content-wrapper  blocks shrinker-parent" style="position:relative;">
 +
<div class="blocks-preview-content-holder shrinker-content">
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="blocks-preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-2a27a910-q8tctoi7" class="preview-element blocks-preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="BLOCKS_PREVIEW_BODY">
 +
<p class="font_8"><span style="color: rgb(0, 0, 0);">Shown are figures of the PCR that amplified the coding sequences for OR2W1 and OR10G4, and images of gels for the restriction enzyme digests confirming the production of the OR10G4 in pSB1C3 and OR2W1 in pSB1C3 deliverables.</span></p><p class="font_8"><span style="color: rgb(0, 0, 0);"><span class="wixGuard">​</span></span></p><p class="font_8"><span style="color: rgb(0, 0, 0);">For the PCR reactions, the expected bands for the OR10G4 and OR2W1 are both about 0.95kb (above).</span></p><p class="font_8"><span style="color: rgb(0, 0, 0);"><span class="wixGuard">​</span></span></p><p class="font_8"><span style="color: rgb(0, 0, 0);">For the OR10G4 restriction enzyme digest reactions, the expected products are (Below): 2.3kb and 0.7kb for the EcoRI and BamHI reaction, 2.3kb and 0.7kb for the BlpI and PstI reaction.</span></p><p class="font_8"><br></p><p class="font_8"><span style="color: rgb(0, 0, 0);">For the OR2W1 restriction enzyme digest reactions, the expected products are (left):2.2kb and 0.8kb for the BglII and PstI reaction, 1.8kb and 1.2kb for the MscI reaction.</span></p><p class="font_8"><span style="color: rgb(0, 0, 0);"><span class="wixGuard">​</span></span></p><p class="font_8"><span style="color: rgb(0, 0, 0);">Additionally, since olfactory receptors are from the same family of proteins, other sequences were examined under restriction enzyme digest but not inserted into the pSB1C3 plasmid (below).</span></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="blocks"></div>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-2a27a910-oal9bp0r"  class="sub item-box  page-box style-2a27a910-2asblupt          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-2a27a910-2asblupt" data-preview-styleid='style-2a27a910-2asblupt' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-2a27a910-oal9bp0r" data-preview-style="style-2a27a910-2asblupt" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="1742" data-orig-thumb-width="867" data-vbid="vbid-2a27a910-oal9bp0r" data-bgimg="https://lh3.googleusercontent.com/HRE7sHYap4xcQtL-Y17xARv4vBTZ44y_TIZgL95YbWUYA52NCJsBzgmEPce6VupZdS-dobW1AAD9gRG-EQ">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div bottom-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-2a27a910-piwxyxqa-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-2a27a910-piwxyxqa" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/HRE7sHYap4xcQtL-Y17xARv4vBTZ44y_TIZgL95YbWUYA52NCJsBzgmEPce6VupZdS-dobW1AAD9gRG-EQ=s300);"  data-orig-width="867" data-orig-height="1742" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 +
 +
<div class="preview-form order-handle">
 +
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div  id="vbid-2a27a910-y70abt8o"  class="sub item-box  page-box style-2a27a910-2asblupt          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-2a27a910-2asblupt" data-preview-styleid='style-2a27a910-2asblupt' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-2a27a910-y70abt8o" data-preview-style="style-2a27a910-2asblupt" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="1753" data-orig-thumb-width="895" data-vbid="vbid-2a27a910-y70abt8o" data-bgimg="https://lh3.googleusercontent.com/602Ea2D1TPGR-PXfdK57eQJ-UDbQ3UKINAMtsB-GwQbVNp6jF46aKnUyvGCniIvJeAJybHGx111ofMBe5o0">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div bottom-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-2a27a910-ccibivql-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-2a27a910-ccibivql" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/602Ea2D1TPGR-PXfdK57eQJ-UDbQ3UKINAMtsB-GwQbVNp6jF46aKnUyvGCniIvJeAJybHGx111ofMBe5o0=s300);"  data-orig-width="895" data-orig-height="1753" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
 +
<!--  BY NATIVE ORDER -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div>
 +
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div>
 +
 +
 +
<div class="preview-form order-handle">
 +
 +
<div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div>
 +
</div>
 +
 +
<div class="preview-item-links order-handle ">
 +
<div class="preview-links-wrapper">
 +
 +
<div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
<div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- PROJECTS END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-f9fd6004-yoc8wzdr"  class="master item-box  gallery-box style-f9fd6004-hpejdaa5    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-f9fd6004-hpejdaa5" data-preview-styleid='style-f9fd6004-hpejdaa5' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-f9fd6004-hpejdaa5 content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-f9fd6004-hpejdaa5" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-f9fd6004-yoc8wzdr" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-f9fd6004-hpejdaa5" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-f9fd6004-our3bqlf"  class="sub item-box  page-box style-f9fd6004-hpejdaa5          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-f9fd6004-hpejdaa5" data-preview-styleid='style-f9fd6004-hpejdaa5' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-f9fd6004-our3bqlf" data-preview-style="style-f9fd6004-hpejdaa5" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-f9fd6004-our3bqlf" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-f9fd6004-bi6y1lze" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="font_8" style="line-height: 1.8em;line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);">For construction of iGEM deliverables containing odorant receptor proteins in the pSB1C3 plasmid, the coding sequences for OR10G4 and OR2W1 were amplified by PCR using the respective pCMV6 and pCI mammalian expression plasmids as templates. The PCR reactions were expected to generate bands of approximately 0.95kb for both OR10G4 and OR2W1 coding sequences, and the image of the gel for the PCR products shows bands at this expected size. These PCR products were amplified using primers that incorporated EcoRI and PstI restriction endonuclease sites at the 5’ and 3’ ends of the PCR products. Digesting both the PCR products and the pre-linearized pSB1C3 plasmid supplied by iGEM with EcoRI and PstI restriction endonucleases, the PCR products were ligated into the pSB1C3 plasmid. The ligation reaction was used to transform chemically competent DH5alpha cells. To screen for colonies containing the appropriately inserted odorant receptor gene and pSB1C3 plasmid, colonies were used to inoculate small cultures. The plasmids were harvested from the cultures by mini-prep plasmid isolation kits, and the isolated plasmids were digested with two sets of restriction endonuclease reactions. </span></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-e1e92f27-cmzxvy05"  class="master item-box  gallery-box style-e1e92f27-hbfm8aer    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-e1e92f27-hbfm8aer" data-preview-styleid='style-e1e92f27-hbfm8aer' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-e1e92f27-hbfm8aer content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-e1e92f27-hbfm8aer" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-e1e92f27-cmzxvy05" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-e1e92f27-hbfm8aer" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-e1e92f27-bhsp2xpk"  class="sub item-box  page-box style-e1e92f27-hbfm8aer          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-e1e92f27-hbfm8aer" data-preview-styleid='style-e1e92f27-hbfm8aer' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-e1e92f27-bhsp2xpk" data-preview-style="style-e1e92f27-hbfm8aer" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="285" data-orig-thumb-width="997" data-vbid="vbid-e1e92f27-bhsp2xpk" data-bgimg="https://lh3.googleusercontent.com/jkf-5NFNrz7IJV3YMFNOQXWmTDTXXqjnFkq_CwgSTXs8oByIUvRGCp4q672L3L5PSTAVOqUfrApFLlQN">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="element-5bacffa42df66f2-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="element-5bacffa42df66f2" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/jkf-5NFNrz7IJV3YMFNOQXWmTDTXXqjnFkq_CwgSTXs8oByIUvRGCp4q672L3L5PSTAVOqUfrApFLlQN=s300);"  data-orig-width="997" data-orig-height="285" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-79d95830-cmzxvy05"  class="master item-box  gallery-box style-79d95830-hbfm8aer    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-79d95830-hbfm8aer" data-preview-styleid='style-79d95830-hbfm8aer' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-79d95830-hbfm8aer content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-79d95830-hbfm8aer" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-79d95830-cmzxvy05" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-79d95830-hbfm8aer" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-79d95830-bhsp2xpk"  class="sub item-box  page-box style-79d95830-hbfm8aer          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-79d95830-hbfm8aer" data-preview-styleid='style-79d95830-hbfm8aer' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-79d95830-bhsp2xpk" data-preview-style="style-79d95830-hbfm8aer" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="315" data-orig-thumb-width="333" data-vbid="vbid-79d95830-bhsp2xpk" data-bgimg="https://lh3.googleusercontent.com/j-4iz1xFp0ygLwMvkCJIZoFTrcYRQo3PoBnZw5zVaZLceiO_yM5oaV6vAt0Yv4-wmRuvxmlOhovmacCs0Q">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-left" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="element-6f95f460076125a-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="element-6f95f460076125a" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/j-4iz1xFp0ygLwMvkCJIZoFTrcYRQo3PoBnZw5zVaZLceiO_yM5oaV6vAt0Yv4-wmRuvxmlOhovmacCs0Q=s300);"  data-orig-width="333" data-orig-height="315" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="element-a2ede40f1f1fdb0" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Animal Cell Nucleofection</h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-79d95830-nrrlvpof" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="font_8" style="line-height: 1.8em;line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);">Transferring the olfactory receptor into animal cells is critical component to the development of a working biosensor. However, determining the production of the olfactory receptor was determined by three methods: 1) Western Blotting to determine if the olfactory receptor protein was produced, 2) immunohistochemistry ensure the receptor was transported to the membrane, and 3) physiological response of cell action potentials in the presence of specific odorants.</span><br></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-fca7af2b-wdug7ril"  class="master item-box  gallery-box style-fca7af2b-rmga8dub    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-fca7af2b-rmga8dub" data-preview-styleid='style-fca7af2b-rmga8dub' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-fca7af2b-rmga8dub content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-fca7af2b-rmga8dub" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-fca7af2b-wdug7ril" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-fca7af2b-rmga8dub" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-fca7af2b-uci1vwwx"  class="sub item-box  page-box style-fca7af2b-rmga8dub          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-fca7af2b-rmga8dub" data-preview-styleid='style-fca7af2b-rmga8dub' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-fca7af2b-uci1vwwx" data-preview-style="style-fca7af2b-rmga8dub" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="826" data-orig-thumb-width="975" data-vbid="vbid-fca7af2b-uci1vwwx" data-bgimg="https://lh3.googleusercontent.com/uoYV_wqDyzIA_MV77L_X93gpNb0rAp64iWphTW5EyYluZhV_MozanQohEMuuWTykbkW-1mHAEEeilFSEqQ">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-right" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-fca7af2b-ymbtlvpb-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-fca7af2b-ymbtlvpb" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/uoYV_wqDyzIA_MV77L_X93gpNb0rAp64iWphTW5EyYluZhV_MozanQohEMuuWTykbkW-1mHAEEeilFSEqQ=s300);"  data-orig-width="975" data-orig-height="826" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-fca7af2b-xzmbq6fa" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Western Blot<br></h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-fca7af2b-dqcuugre" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="font_8" style="line-height: 1.8em;line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);">Sample ORs were selected and analyzed via Western Blotting to determine the size and purity of the antigen protein. The image below is a Western Blot of  HT-22 Cells. From left to right: Ladder, Ladder, Control, GPF, OR5P3, OR5A1, OR51E1, OR02C1 OR10G4, and OR2W1. The green bands at ~37 kDa prove that OR proteins were produced after 3 days in culture meaning that primary and secondary antibodies matched and bound to target OR antigens.</span><br></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-5a2a12a1-chqdlofs"  class="master item-box  gallery-box style-5a2a12a1-hem4fpm2    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-5a2a12a1-hem4fpm2" data-preview-styleid='style-5a2a12a1-hem4fpm2' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-5a2a12a1-hem4fpm2 content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-5a2a12a1-hem4fpm2" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-5a2a12a1-chqdlofs" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-5a2a12a1-hem4fpm2" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-5a2a12a1-7bw6yzbn"  class="sub item-box  page-box style-5a2a12a1-hem4fpm2          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-5a2a12a1-hem4fpm2" data-preview-styleid='style-5a2a12a1-hem4fpm2' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-5a2a12a1-7bw6yzbn" data-preview-style="style-5a2a12a1-hem4fpm2" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="1083" data-orig-thumb-width="1027" data-vbid="vbid-5a2a12a1-7bw6yzbn" data-bgimg="https://lh3.googleusercontent.com/U5eQ19FXrgby_pktE9AuVPRTn1gw1ru-7MJb3cIWFbvpBi0M7NHUSwSIyfYYDtV1W8zRrCepJ4ECVgzvuA">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-left" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-5a2a12a1-5skxobjw-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-5a2a12a1-5skxobjw" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/U5eQ19FXrgby_pktE9AuVPRTn1gw1ru-7MJb3cIWFbvpBi0M7NHUSwSIyfYYDtV1W8zRrCepJ4ECVgzvuA=s300);"  data-orig-width="1027" data-orig-height="1083" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-5a2a12a1-blubujj3" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Immunohistochemistry<br></h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-5a2a12a1-ff2lsin5" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="font_8" style="line-height: 1.8em;line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);">OR transfected cells were also captured, displayed and analyzed via fluorescence and confocal microscopy. Primary immunohistochemistry exploits a target OR antigen, while secondary immunohistochemistry proves the initial attachment of the primary antibody. The images below show both fluorescence (top) and confocal (bottom) microscopy.</span><br></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-a6a68d49-gpcjtyfd"  class="master item-box  gallery-box style-a6a68d49-c5togdxp    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-a6a68d49-c5togdxp" data-preview-styleid='style-a6a68d49-c5togdxp' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-a6a68d49-c5togdxp content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-a6a68d49-c5togdxp" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-a6a68d49-gpcjtyfd" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-a6a68d49-c5togdxp" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-a6a68d49-7yzoe8fz"  class="sub item-box  page-box style-a6a68d49-c5togdxp          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-a6a68d49-c5togdxp" data-preview-styleid='style-a6a68d49-c5togdxp' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-a6a68d49-7yzoe8fz" data-preview-style="style-a6a68d49-c5togdxp" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="798" data-orig-thumb-width="1552" data-vbid="vbid-a6a68d49-7yzoe8fz" data-bgimg="https://lh3.googleusercontent.com/xeSxAKqcQpCHXzqN1ZspkCFKvzFCnz5I1ucnF5ZZQfsxtzhslloWtD1fpzVgkoTsqmGeaW736Vmgx18e-zc">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-a6a68d49-4wmhf9bp-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-a6a68d49-4wmhf9bp" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/xeSxAKqcQpCHXzqN1ZspkCFKvzFCnz5I1ucnF5ZZQfsxtzhslloWtD1fpzVgkoTsqmGeaW736Vmgx18e-zc=s300);"  data-orig-width="1552" data-orig-height="798" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-7e3671b9-4r2l0fh3"  class="master item-box  gallery-box style-7e3671b9-9eb964jp    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-7e3671b9-9eb964jp" data-preview-styleid='style-7e3671b9-9eb964jp' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-7e3671b9-9eb964jp content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-7e3671b9-9eb964jp" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-7e3671b9-4r2l0fh3" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-7e3671b9-9eb964jp" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-7e3671b9-3s6xin9f"  class="sub item-box  page-box style-7e3671b9-9eb964jp          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-7e3671b9-9eb964jp" data-preview-styleid='style-7e3671b9-9eb964jp' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-7e3671b9-3s6xin9f" data-preview-style="style-7e3671b9-9eb964jp" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="1110" data-orig-thumb-width="1199" data-vbid="vbid-7e3671b9-3s6xin9f" data-bgimg="https://lh3.googleusercontent.com/rWFOx79k7Rjp0XhGapMOGCPfyP9KqpC6EmwgDvdLJtNzq0DLoVX1JFlWSKFORVhzmIQbz4WO14UFWSxMZg">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-left" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-7e3671b9-tjcmadc1-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-7e3671b9-tjcmadc1" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/rWFOx79k7Rjp0XhGapMOGCPfyP9KqpC6EmwgDvdLJtNzq0DLoVX1JFlWSKFORVhzmIQbz4WO14UFWSxMZg=s300);"  data-orig-width="1199" data-orig-height="1110" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-7e3671b9-qh1fhrsb" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Physiological Response<br></h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-7e3671b9-ik0osf1h" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="font_8" style="line-height: 1.8em;line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);">HT-22 cells were cultured on an Axion Biosystems Microelectrode Array cell culture dish to measure field potential measurements of depolarization. HT-22 cells nucleofected with OR 2W1 were presented with hexanol, a positive ligand for the OR 2W1 receptor. Multiple additions were made in 1 minute increments  revealing the resetting capability and max depolarization of the HT-22 cells.</span><br></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-d3d8cab0-thaf28me"  class="master item-box  gallery-box style-d3d8cab0-9lu3hgn3    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-d3d8cab0-9lu3hgn3" data-preview-styleid='style-d3d8cab0-9lu3hgn3' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-d3d8cab0-9lu3hgn3 content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-d3d8cab0-9lu3hgn3" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-d3d8cab0-thaf28me" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-d3d8cab0-9lu3hgn3" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-d3d8cab0-6amrtax9"  class="sub item-box  page-box style-d3d8cab0-9lu3hgn3          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-d3d8cab0-9lu3hgn3" data-preview-styleid='style-d3d8cab0-9lu3hgn3' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-d3d8cab0-6amrtax9" data-preview-style="style-d3d8cab0-9lu3hgn3" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="342" data-orig-thumb-width="194" data-vbid="vbid-d3d8cab0-6amrtax9" data-bgimg="https://lh3.googleusercontent.com/YU26kQ2PX4Tu4gd2yWLV9SgkKPc2mIeQKaHotmPH-ZowVN4Q3PGqbHjJF51L0rtBX5bV2QGrumouptUo">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-right" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-d3d8cab0-dzmhjmhd-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-d3d8cab0-dzmhjmhd" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/YU26kQ2PX4Tu4gd2yWLV9SgkKPc2mIeQKaHotmPH-ZowVN4Q3PGqbHjJF51L0rtBX5bV2QGrumouptUo=s300);"  data-orig-width="194" data-orig-height="342" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-d3d8cab0-la0t59sc" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Handheld Photosensor Device<br></h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-d3d8cab0-ozbffr0k" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="font_8" style="line-height: 1.8em;line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);">Two prototypes have been designed and developed capable of utilizing different methods of biosensor detection. One of the devices utilizes a photo sensor connected to an Arduino, translating the cell action potentials into plain text. </span><br></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- TEXT_BLOCK START -->
 +
<div  id="vbid-b5ab922d-asjkfogu"  class="master item-box  gallery-box style-b5ab922d-vjkbyikp    button-effects btn_hover4      " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="STYLE"  data-styleid="style-b5ab922d-vjkbyikp" data-preview-styleid='style-b5ab922d-vjkbyikp' data-preset-type-id="TEXT_BLOCK">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper text_block-wrapper ">
 +
 +
<div  class="sub container style-b5ab922d-vjkbyikp content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-b5ab922d-vjkbyikp" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-b5ab922d-asjkfogu" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-b5ab922d-vjkbyikp" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-b5ab922d-zhtxv6c3"  class="sub item-box  page-box style-b5ab922d-vjkbyikp          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-b5ab922d-vjkbyikp" data-preview-styleid='style-b5ab922d-vjkbyikp' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-b5ab922d-zhtxv6c3" data-preview-style="style-b5ab922d-vjkbyikp" data-style="style-4e411-yq39oaomol" data-orig-thumb-height="2448" data-orig-thumb-width="3264" data-vbid="vbid-b5ab922d-zhtxv6c3" data-bgimg="https://lh3.googleusercontent.com/QHSAwkW0XSySx6g622HYaMSOpJFXXV0r9s8rlo8o1-v80ve4capgKL7f8qPZQ6bpCxG-g_FLblO6dAxCCA">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-left" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="vbid-b5ab922d-x3xhy95r-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="vbid-b5ab922d-x3xhy95r" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/QHSAwkW0XSySx6g622HYaMSOpJFXXV0r9s8rlo8o1-v80ve4capgKL7f8qPZQ6bpCxG-g_FLblO6dAxCCA=s300);"  data-orig-width="3264" data-orig-height="2448" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-b5ab922d-ft2uybjm" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Microelectrode Array Bioreactor<br></h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-body-holder removable-parent order-handle">
 +
 +
<div id="vbid-b5ab922d-sxwurjya" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_BODY">
 +
<p class="font_8" style="line-height: 1.5em;"><span style="color: rgb(0, 0, 0);">In a controlled environment, neurons with enhanced olfactory receptors are cultured on a microelectrode array. While media is flowed through via a peristaltic pump, certain olfactants are injected. When the neurons detect these olfactants, they produce an electrical signal, which is then transferred to a computer for interpretation.</span></p><p class="font_8" style="line-height: 1.5em;"><span style="color: rgb(0, 0, 0);"><span class="wixGuard">​</span></span></p><p class="font_8" style="line-height: 1.5em;"><span style="color: rgb(0, 0, 0);">After conducting multiple trials, it was shown that there was no significant increase in voltage reading the olfactant was introduced into the closed system. This may have been because the plasmid is not being expressed sufficiently, or that 24 hours after transfection was not the optimal time to conduct trials. There was also no reference electrode, which could have altered the computers interpretation of the voltage data</span><br></p>
 +
</div>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- TEXT_BLOCK END -->
 +
 +
 +
 +
 +
<!-- FOOTERS START -->
 +
<div  id="vbid-7d38c6a1-vhgfqwrt"  class="master item-box  gallery-box style-7d38c6a1-sr6fbz1e injected        " data-holder-type="gallery"" data-holder-type="gallery"  data-child-type="ORIGIN"  data-styleid="style-7d38c6a1-sr6fbz1e" data-preview-styleid='style-7d38c6a1-sr6fbz1e' data-preset-type-id="FOOTERS">
 +
<div id="no-image" class="stripe-background load-high-res " ></div>
 +
<div class="gallery-wrapper item-wrapper footers-wrapper ">
 +
 +
<div  class="sub container style-7d38c6a1-sr6fbz1e content flex  " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy"  data-itemslug="loading-copy" data-itemstyleid="style-7d38c6a1-sr6fbz1e" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-7d38c6a1-vhgfqwrt" data-preset-type-id="FOOTERS" data-preview-style="style-7d38c6a1-sr6fbz1e" data-style="style-f3095-j0xhjgzynh" data-absolute-path="/free/amitro02"  >
 +
<!-- ARRANGER SETTINGS -->
 +
<div class="arranger-settings" style="display:none;"
 +
data-ARRANGER_TYPE="flex"
 +
data-ARRANGER_COLS="0"
 +
data-ARRANGER_ITEM_MAX_WIDTH="400"
 +
data-ARRANGER_ITEM_MIN_WIDTH="230"
 +
data-ARRANGER_ITEM_RATIO="1"
 +
data-ARRANGER_ITEM_SPACING="20"
 +
data-ARRANGER_ITEMS_PER_PAGE="all"
 +
data-ARRANGER_ORDER_TYPE="regular"
 +
data-AUTO_PLAY="true"
 +
data-AUTO_PLAY_DURATION="4"
 +
data-FLEX_ELEMENT_EFFECT="effect-fadein"
 +
></div>
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
<div id="children">
 +
 +
 +
<div id="items-holder-wrapper">
 +
<div id="items-holder">
 +
 +
 +
 +
 +
<div  id="vbid-7d38c6a1-eqit56l5"  class="sub item-box  page-box style-7d38c6a1-sr6fbz1e          " data-holder-type="page"" data-holder-type="page"  data-child-type="STYLE"  data-styleid="style-7d38c6a1-sr6fbz1e" data-preview-styleid='style-7d38c6a1-sr6fbz1e' data-preset-type-id="UNRESOLVED">
 +
 +
<div class="page-wrapper item-wrapper ">
 +
 +
<div class="item-content leaf multi_layout page content -container" data-self="vbid-7d38c6a1-eqit56l5" data-preview-style="style-7d38c6a1-sr6fbz1e" data-style="style-f3095-yq39oaomol" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-7d38c6a1-eqit56l5" data-bgimg="">
 +
<div  class="multi-container preview image-cover" >
 +
<div class="Picture item-preview">
 +
<div class="preview-image-holder">
 +
<div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style="">
 +
</div>
 +
<div class="helper-div middle-center" >
 +
<!-- <div class="benet" style="min-height:inherit;"></div> -->
 +
<div class="pic-side">
 +
<div class="vertical-aligner">
 +
<div id="no-image-holder"  class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER">
 +
 +
<div  id="no-image" class="inner-pic preview-element  magic-circle-holder  load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style=""  data-orig-width="" data-orig-height="" >
 +
<!-- VIDEO TEMPLATE -->
 +
 +
 +
<div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div>
 +
 +
 +
<!-- MAP TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div>
 +
 +
<!-- RAW TEMPLATE -->
 +
 +
<div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div>
 +
 +
</div>
 +
 +
</div>
 +
 +
 +
</div>
 +
</div>
 +
<div class="text-side shrinker-parent">
 +
<div class="vertical-aligner">
 +
<div class="item-details preview-content-wrapper  multi" style="position:relative;">
 +
<div class="draggable-div-holder"></div>
 +
<div class="preview-content-holder shrinker-content">
 +
 +
<!--  BY SPECIFIC ORDER -->
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-title-holder removable-parent order-handle">
 +
 +
<h2 id="vbid-7d38c6a1-jkaeeacm" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >DEPARTMENT OF CHEMISTRY AND LIFE SCIENCE<br></h2>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-subtitle-holder removable-parent order-handle">
 +
 +
<h3 id="vbid-7d38c6a1-1dorqubw" class="preview-element preview-subtitle magic-circle-holder text-element quick-text-style-menu  allow-mobile-hide" data-menu-name="PREVIEW_SUBTITLE">BARTLETT HALL, ROOM 400, WEST POINT, NY 10996, USA</h3>
 +
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
 +
<div class="preview-item-links order-handle removable-parent" style="display:inline-block;">
 +
 +
<span id="vbid-7d38c6a1-ynlsfdhm"  class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >TEL: 845.938.3915</span>
 +
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<br class="upper-line-break" />
 +
<div class="preview-social-wrapper removable-parent order-handle"  >
 +
<div id="vbid-7d38c6a1-bdvlps7u" class="preview-element preview-social-holder magic-circle-holder" data-menu-name="PREVIEW_SOCIAL" data-theme="8" >
 +
 +
<div id="FACEBOOK" class='link-entry ' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="FACEBOOK" data-img-url="//www.imcreator.com/images/socialmedia/8facebook.png">
 +
<a class='social-link-url' href="https://www.facebook.com/West-Point-Department-of-Chemistry-and-Life-Science-385389195808/" target='_blank'><img class='preview-link-img ' src="//www.imcreator.com/images/socialmedia/8facebook.png" ></a>
 +
</div>
 +
 +
<div id="TWITTER" class='link-entry ' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="TWITTER" data-img-url="//www.imcreator.com/images/socialmedia/8twitter.png">
 +
<a class='social-link-url' href="https://twitter.com/WestPoint_USMA?ref_src=twsrc%5Egoogle%7Ctwcamp%5Eserp%7Ctwgr%5Eauthor" target='_blank'><img class='preview-link-img ' src="//www.imcreator.com/images/socialmedia/8twitter.png" ></a>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<br class="lower-line-break" />
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<!-- LAYOUT SETTINGS -->
 +
<div class="layout-settings" style="display:none;" data-type="multi"></div>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!-- FOOTERS END -->
 +
 +
 +
 +
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
<div class="light-box-wrapper  space-layer" style="display:none;">
 +
<div class="light-box-image-holder">
 +
<div class="light-box-image animated">
 +
 +
</div>
 +
<div class="lightbox-text-wrapper ">
 +
<div class="lightbox-text-holder animated">
 +
<div class="lightbox-title"></div>
 +
<div class="lightbox-subtitle"></div>
 +
<div id="paginator" style="display:none;">
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
    <img src="https://lh3.googleusercontent.com/EWqW7DEI4kOTRMLjK2-ObFHp-EYBt5apFYZ1LVFAhLtTLjigCRfx5hCCTKbIjIm68VQ00p9twloHJ9w8=s50" class="download-gallery-btn clickable" style="display: none;">
 +
<img src="https://lh3.googleusercontent.com/TgRyMQvJ3_h9RmOnu7AlhIE7NLOOBsRoBounARrs8fQv8HCRPaFtpBneSqJOSZpI6l7He_bAZKN179JBig=s50" class="close-lightbox-btn clickable" style="opacity: 1;">
 +
<img src="https://lh3.googleusercontent.com/43-pXHjwrpmVO8Oean-6BD0uzARvcqUQrpdi7Yw2bxaXwEoP21UdN5kW6Ks9pdOxf7ropMUrh0djgYPwYPU=s50" class="lightbox-arrow lightbox-left clickable top-layer" >
 +
<img src="https://lh3.googleusercontent.com/9rwgVnDglPdPFugSu98fhDmxzjXC9KovZ_7BuHkXPIv6jvg9S96flGnhL_e4y8mIpPpZQstfqEV-WitY=s50" class="lightbox-arrow lightbox-right clickable top-layer" >
 +
</div>
  
 +
</div>
 +
 +
<script src="//www.imcreator.com/js/lightbox.js?v=1.4.1f"  type="text/javascript"></script>
 +
<script src="//www.imcreator.com/js/spimeengine.js?v=1.4.1f"  type="text/javascript"></script>
 +
 +
 +
<!--  SHOPROCKET  ECOMMERCE  -->
 +
<script>
 +
var JSR = jQuery.noConflict();
 +
var $ = jQuery.noConflict();
 +
</script>
 +
 +
<input type="hidden" name="sr-companyid" id="sr-companyid" value="">
 +
</body>
  
 +
 
</html>
 
</html>

Revision as of 04:41, 1 November 2017

HOME
TEAM
PROJECT
Project
Description
Design
Experiments
Notebook
InterLab
Results
Demonstrate
Attributions
DELIVERABLES
PARTS
Parts
Basic Parts
Part Collection
SAFETY
HUMAN PRACTICES
Integrated and Silver/Gold
Public Engagement
AWARDS
Entrepreneurship
Applied Design
Hardware
JUDGING FORM

USMA-West_Point

<!DOCTYPE html> Results - iGem 01

usMA-WEST POINT


Results



Here we present results from three different components: 1) Plasmid design and development, 2) Animal Cell Nucleofection, and 3) Device design.



Plasmid Design and Development



Plasmid design and development is a key component of the eNose project. Since odorant receptor protein function was tested in a mammalian cell line, the plasmids used to drive expression of these proteins were specifically designed for mammalian cells. For example, the odorant receptor proteins OR10G4 and OR2W1 were expressed using the pCMV6 and pCI plasmids, respectively, and both contain a cytomegalovirus (CMV) promoter region for high gene expression levels in mammalian cells.



Shown are figures of the PCR that amplified the coding sequences for OR2W1 and OR10G4, and images of gels for the restriction enzyme digests confirming the production of the OR10G4 in pSB1C3 and OR2W1 in pSB1C3 deliverables.

​

For the PCR reactions, the expected bands for the OR10G4 and OR2W1 are both about 0.95kb (above).

​

For the OR10G4 restriction enzyme digest reactions, the expected products are (Below): 2.3kb and 0.7kb for the EcoRI and BamHI reaction, 2.3kb and 0.7kb for the BlpI and PstI reaction.


For the OR2W1 restriction enzyme digest reactions, the expected products are (left):2.2kb and 0.8kb for the BglII and PstI reaction, 1.8kb and 1.2kb for the MscI reaction.

​

Additionally, since olfactory receptors are from the same family of proteins, other sequences were examined under restriction enzyme digest but not inserted into the pSB1C3 plasmid (below).



For construction of iGEM deliverables containing odorant receptor proteins in the pSB1C3 plasmid, the coding sequences for OR10G4 and OR2W1 were amplified by PCR using the respective pCMV6 and pCI mammalian expression plasmids as templates. The PCR reactions were expected to generate bands of approximately 0.95kb for both OR10G4 and OR2W1 coding sequences, and the image of the gel for the PCR products shows bands at this expected size. These PCR products were amplified using primers that incorporated EcoRI and PstI restriction endonuclease sites at the 5’ and 3’ ends of the PCR products. Digesting both the PCR products and the pre-linearized pSB1C3 plasmid supplied by iGEM with EcoRI and PstI restriction endonucleases, the PCR products were ligated into the pSB1C3 plasmid. The ligation reaction was used to transform chemically competent DH5alpha cells. To screen for colonies containing the appropriately inserted odorant receptor gene and pSB1C3 plasmid, colonies were used to inoculate small cultures. The plasmids were harvested from the cultures by mini-prep plasmid isolation kits, and the isolated plasmids were digested with two sets of restriction endonuclease reactions.



Animal Cell Nucleofection



Transferring the olfactory receptor into animal cells is critical component to the development of a working biosensor. However, determining the production of the olfactory receptor was determined by three methods: 1) Western Blotting to determine if the olfactory receptor protein was produced, 2) immunohistochemistry ensure the receptor was transported to the membrane, and 3) physiological response of cell action potentials in the presence of specific odorants.



Western Blot



Sample ORs were selected and analyzed via Western Blotting to determine the size and purity of the antigen protein. The image below is a Western Blot of HT-22 Cells. From left to right: Ladder, Ladder, Control, GPF, OR5P3, OR5A1, OR51E1, OR02C1 OR10G4, and OR2W1. The green bands at ~37 kDa prove that OR proteins were produced after 3 days in culture meaning that primary and secondary antibodies matched and bound to target OR antigens.



Immunohistochemistry



OR transfected cells were also captured, displayed and analyzed via fluorescence and confocal microscopy. Primary immunohistochemistry exploits a target OR antigen, while secondary immunohistochemistry proves the initial attachment of the primary antibody. The images below show both fluorescence (top) and confocal (bottom) microscopy.



Physiological Response



HT-22 cells were cultured on an Axion Biosystems Microelectrode Array cell culture dish to measure field potential measurements of depolarization. HT-22 cells nucleofected with OR 2W1 were presented with hexanol, a positive ligand for the OR 2W1 receptor. Multiple additions were made in 1 minute increments revealing the resetting capability and max depolarization of the HT-22 cells.



Handheld Photosensor Device



Two prototypes have been designed and developed capable of utilizing different methods of biosensor detection. One of the devices utilizes a photo sensor connected to an Arduino, translating the cell action potentials into plain text.



Microelectrode Array Bioreactor



In a controlled environment, neurons with enhanced olfactory receptors are cultured on a microelectrode array. While media is flowed through via a peristaltic pump, certain olfactants are injected. When the neurons detect these olfactants, they produce an electrical signal, which is then transferred to a computer for interpretation.

​

After conducting multiple trials, it was shown that there was no significant increase in voltage reading the olfactant was introduced into the closed system. This may have been because the plasmid is not being expressed sufficiently, or that 24 hours after transfection was not the optimal time to conduct trials. There was also no reference electrode, which could have altered the computers interpretation of the voltage data



DEPARTMENT OF CHEMISTRY AND LIFE SCIENCE



BARTLETT HALL, ROOM 400, WEST POINT, NY 10996, USA


TEL: 845.938.3915