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− | |||
+ | <!DOCTYPE html> | ||
+ | <html xmlns="http://www.w3.org/1999/xhtml"> | ||
+ | <head><meta name='robots' content='noindex, nofollow'> | ||
+ | <title> | ||
+ | Results - iGem 01</title> | ||
+ | <!-- META --> | ||
+ | <meta charset="UTF-8"> | ||
+ | <meta name="viewport" content="width=device-width, maximum-scale=1"/> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <script src="https://www.youtube.com/iframe_api"></script> | ||
+ | <!-- CSS --> | ||
+ | <link rel="stylesheet" href="//www.imcreator.com/css/common.css?v=1.4.1f"> | ||
+ | <link rel="stylesheet" type="text/css" href="//www.imcreator.com/all_css.css?v=1.4.1f" /> | ||
− | < | + | |
+ | |||
+ | <link rel="icon" type="image/png" href="https://lh3.googleusercontent.com/dd_e0xA19up9208Tv6odcjHEw6z4cKAA6fTgjZ9ynkKoSnr5R4vFxI7gZp6pnZH1Vi5T5f-fphjFsrLG=s30" /> | ||
+ | <link rel="apple-touch-icon" href="https://lh3.googleusercontent.com/dd_e0xA19up9208Tv6odcjHEw6z4cKAA6fTgjZ9ynkKoSnr5R4vFxI7gZp6pnZH1Vi5T5f-fphjFsrLG=s30" /> | ||
+ | |||
+ | |||
+ | |||
+ | <link rel="stylesheet" type="text/css" href="https://maxcdn.bootstrapcdn.com/font-awesome/4.7.0/css/font-awesome.min.css" /> | ||
+ | |||
+ | <link id="vbid-5734f2ef-jztngyge-STRIPE_DATA" rel="stylesheet" type="text/css" href="//www.imcreator.com/stripe_data_css?page_id=vbid-5734f2ef-jztngyge-STRIPE_DATA&v=1.4.1f"> | ||
+ | |||
+ | <link id="vbid-7d38c6a1-ojqdlra3-STRIPE_DATA" rel="stylesheet" type="text/css" href="//www.imcreator.com/stripe_data_css?page_id=vbid-7d38c6a1-ojqdlra3-STRIPE_DATA&v=1.4.1f"> | ||
+ | |||
+ | <link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/fonts.css?v=1.4.1f" /> | ||
+ | <link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/effects.css?v=1.4.1f" /> | ||
+ | <link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/lightbox.css?v=1.4.1f"> | ||
+ | |||
+ | <link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/previewhelper.css?v=1.4.1f"> | ||
+ | |||
+ | <link rel="stylesheet" type="text/css" href="//www.imcreator.com/css/spimeview.css?v=1.4.1f"> | ||
+ | |||
+ | <link id="vbid-5734f2ef-jztngyge-STATIC_STYLE" rel="stylesheet" type="text/css" href="//www.imcreator.com/static_style?v=1.4.1f&vbid=vbid-5734f2ef-jztngyge&caller=static"> | ||
+ | |||
+ | |||
+ | <!-- SCRIPT --> | ||
+ | <script src="https://code.jquery.com/jquery-2.x-git.min.js" type="text/javascript"></script> | ||
+ | <script src="https://maps.googleapis.com/maps/api/js?key=AIzaSyByWRZWFbQRxJM7SiNz2NURPDPJPqTtBQQ" type="text/javascript"></script> | ||
+ | |||
+ | <script src="//www.imcreator.com/js/xprs_helper.js?v=1.4.1f" type="text/javascript"></script> | ||
+ | |||
+ | <script src="https://imos004-dot-im--os.appspot.com/js/imos.js?v=1.4.1f" type="text/javascript"></script> | ||
− | < | + | <script type="text/javascript" src="//www.imcreator.com/all_js.js?v=1.4.1f"></script> |
+ | <script src="//www.imcreator.com/js/lib/touchswipe/jquery.mobile.custom.min.js"></script> | ||
+ | <script type="text/javascript">IMOS.pageView();</script> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <script src="//www.imcreator.com/js/preview_helper.js?v=1.4.1f" type="text/javascript"></script> | ||
+ | |||
+ | |||
+ | <!-- User analytics --> | ||
− | |||
− | < | + | <!-- Serving analytics --> |
− | < | + | <script> |
− | + | var currentDomain = window.location.host; | |
− | < | + | if (currentDomain != "localhost:7000"){ |
− | + | var _gaq = _gaq || []; | |
− | </ | + | _gaq.push(['_setAccount', 'UA-58695071-1']); |
+ | _gaq.push(['_setDomainName', currentDomain]); | ||
+ | _gaq.push(['_setAllowLinker', true]); | ||
+ | _gaq.push(['_trackPageview']); | ||
+ | } | ||
+ | </script> | ||
+ | |||
+ | <!-- Label config--> | ||
+ | <script> | ||
+ | |||
+ | var LABEL_CONFIG = {}; | ||
+ | |||
+ | </script></head> | ||
− | < | + | <body class="fast-animated-bg pattern-bg " data-ecommerce-solution="NONE" data-root-id="vbid-7d38c6a1-ojqdlra3" data-root-style-id="style-7d38c6a1-d2bgnenh" data-default-currency="USD" data-osid="osid--ec2fa157-ff75c294" data-app-version="1.4.1f" data-caller="static" data-ecommerce-dashboard="https://dashboard.shoprocket.co" data-static-server="//www.imcreator.com" data-imos-server="https://imos004-dot-im--os.appspot.com"> |
+ | |||
+ | <div id="xprs" data-website-name="iGem 01" class="xprs-holder" > | ||
+ | <div class="main-page " > | ||
+ | <div id="content"> | ||
+ | <div id="vbid-5734f2ef-jztngyge" class="master container style-5734f2ef-9z1ag17a content stripes website-style " data-itemtype="folder" data-creator="" data-itemname="Results" data-itemslug="results" data-itemstyleid="style-5734f2ef-9z1ag17a" data-margintop="" data-arranger="stripes" data-layout="multi" data-vbid="vbid-5734f2ef-jztngyge" data-preset-type-id="ITEMS" data-preview-style="style-5734f2ef-9z1ag17a" data-style="style-7f715-fyu5oogf7j" data-absolute-path="/free/amitro02" > | ||
+ | <!-- ARRANGER SETTINGS --> | ||
+ | <div class="arranger-settings" style="display:none;" | ||
+ | data-ARRANGER_TYPE="stripes" | ||
+ | data-ARRANGER_COLS="0" | ||
+ | data-ARRANGER_ITEM_MAX_WIDTH="400" | ||
+ | data-ARRANGER_ITEM_MIN_WIDTH="230" | ||
+ | data-ARRANGER_ITEM_RATIO="1" | ||
+ | data-ARRANGER_ITEM_SPACING="20" | ||
+ | data-ARRANGER_ITEMS_PER_PAGE="all" | ||
+ | data-ARRANGER_ORDER_TYPE="regular" | ||
+ | data-AUTO_PLAY="false" | ||
+ | data-AUTO_PLAY_DURATION="4" | ||
+ | data-FLEX_ELEMENT_EFFECT="" | ||
+ | ></div> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
+ | <div id="children"> | ||
+ | |||
+ | |||
+ | |||
+ | <!-- MENUS START --> | ||
+ | <div id="vbid-7d38c6a1-8otprari" class="master item-box header-box style-7d38c6a1-8jo8o4ao injected " data-holder-type="header"" data-holder-type="header" data-child-type="ORIGIN" data-styleid="style-7d38c6a1-8jo8o4ao" data-preview-styleid='style-7d38c6a1-8jo8o4ao' data-preset-type-id="MENUS"> | ||
+ | <div id="no-image" class="stripe-background load-high-res " ></div> | ||
+ | <div class="header-wrapper item-wrapper menus-wrapper "> | ||
+ | |||
+ | <div class="item-content leaf menu_layout header content" data-preview-style="style-7d38c6a1-8jo8o4ao" data-style="style-7f715-scjjarqgxu" data-behavior-type="NOTHING" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-7d38c6a1-8otprari" data-bgimg=""> | ||
+ | <div class="preview-content-wrapper"> | ||
+ | <div class="preview-content-holder"> | ||
+ | <div class="left-div"> | ||
+ | <div class="benet" style="min-height:inherit;"></div> | ||
+ | <div class="logo-holder"> | ||
+ | <!-- ICON TEMPLATE --> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div> | ||
− | < | + | </div> |
− | < | + | <div class="helper-div"> |
− | < | + | <div class="item-details menu"> |
− | < | + | <!-- TITLE TEMPLATE --> |
− | </ | + | |
+ | <div class="preview-title-holder removable-parent"> | ||
+ | |||
+ | <a href="/free/amitro02/igem-01" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <h2 id="vbid-7d38c6a1-8ye3bio7" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >usMA-WEST POINT<br></h2> | ||
+ | </a> | ||
+ | </div> | ||
+ | <br /> | ||
+ | <!-- SUBTITLE TEMPLATE --> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="right-div"> | ||
+ | <div class="benet" style="min-height:inherit;"></div> | ||
+ | <!-- LINKS TEMPLATE --> | ||
+ | <div class="preview-item-links "> | ||
+ | <ul class="preview-links-wrapper"> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="vbid-7d38c6a1-0vahew5d" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Home</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/vbid-f02482b7-t3dtwkay" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="c4532978" class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Team</span> | ||
+ | </a> | ||
+ | <ul class="submenu"> | ||
+ | |||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/team" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="e14b181a" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Students, Advisors, PIs, and Instructors</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/collaboration" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="facc214a" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Collaboration</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </ul> | ||
+ | </li class="removable-parent"> | ||
+ | |||
+ | |||
+ | |||
+ | <li> | ||
+ | |||
+ | <span id="element-4cd1f662a476dc8" class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Project</span> | ||
+ | |||
+ | <ul class="submenu"> | ||
+ | |||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/project" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="38c7042b" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Project</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/description" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="42d444bb" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Description</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/design" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="59bf92c2" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Design</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/experiments" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="487700ca" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Experiments</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/notebook" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="ab39f1bd" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Notebook</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/interlab-study" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="21fa8523" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >InterLab Study</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/results" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="cff7eeb1" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Results</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/demonstrate" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="6157824b" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Demonstrate</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/attribution" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="a0b12bde" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Attribution</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <!-- [] --> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/deliverables" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="09c40e1e" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Deliverables</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li> | ||
+ | |||
+ | <span id="element-78132adefe1a4d4" class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Parts</span> | ||
+ | |||
+ | <ul class="submenu"> | ||
+ | |||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/basic-parts" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="fa2fa5ec" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Basic Parts</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/parts-collection" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="37cd96f3" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Parts Collection</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <!-- [] --> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/other" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="99ad6039" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >Safety</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li> | ||
+ | |||
+ | <span id="element-b165365db185e95" class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Human Practices </span> | ||
+ | |||
+ | <ul class="submenu"> | ||
+ | |||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/human-practices" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="61c8e27f" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Integrated and Gold</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/public-engagement" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="f9e986dd" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Public Engagement</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </ul> | ||
+ | </li class="removable-parent"> | ||
+ | |||
+ | |||
+ | |||
+ | <li> | ||
+ | |||
+ | <span id="element-905a6c68c42aebb" class="preview-element Link item-link magic-circle-holder text-element custom submenu-title" data-menu-name="PREVIEW_LINK" >Awards</span> | ||
+ | |||
+ | <ul class="submenu"> | ||
+ | |||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/entreneurship" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="ca6cd864" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Entreneurship</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/extra" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="bc7909bf" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Applied design</span> | ||
+ | </a> | ||
+ | |||
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+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <li class="removable-parent"> | ||
+ | |||
+ | <a class="removable-parent" href="/free/amitro02/igem-01/hardware" data-link-type="EXISTING" target="_self" > | ||
+ | |||
+ | <span id="c5142c05" class="preview-element Link item-link magic-circle-holder text-element custom submenu-link" data-menu-name="PREVIEW_LINK" >Hardware</span> | ||
+ | </a> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | </li> | ||
+ | |||
+ | |||
+ | <!-- [1] --> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
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+ | data-MENU_POSITION="top" | ||
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</div> | </div> | ||
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+ | <!-- ARRANGER SETTINGS --> | ||
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+ | data-FLEX_ELEMENT_EFFECT="effect-fadein" | ||
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+ | <div class="multi-container preview image-cover" > | ||
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+ | <div class="helper-div middle-center" > | ||
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− | < | + | <!-- MAP TEMPLATE --> |
+ | |||
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− | < | + | <!-- RAW TEMPLATE --> |
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− | + | ||
− | + | </div> | |
− | + | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="text-side shrinker-parent"> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div class="item-details preview-content-wrapper multi" style="position:relative;"> | ||
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+ | <div class="preview-content-holder shrinker-content"> | ||
+ | |||
+ | <!-- BY SPECIFIC ORDER --> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <br class="upper-line-break" /> | ||
+ | <div class="preview-title-holder removable-parent order-handle"> | ||
+ | |||
+ | <h1 id="vbid-5734f2ef-4t0dorlc" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Results<br></h1> | ||
+ | |||
</div> | </div> | ||
+ | <br class="lower-line-break" /> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <br class="upper-line-break" /> | ||
+ | <div class="preview-body-holder removable-parent order-handle"> | ||
+ | |||
+ | <div id="vbid-5734f2ef-gehbufaw" class="preview-element preview-body magic-circle-holder text-element quick-text-style-menu allow-mobile-hide" data-menu-name="PREVIEW_BODY"> | ||
+ | <p class="font_7" style="text-align: center; line-height: 1.5em;">Here we present results from three different components: 1) Plasmid design and development, 2) Animal Cell Nucleofection, and 3) Device design.<br></p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <br class="lower-line-break" /> | ||
− | <div class=" | + | |
+ | |||
+ | |||
+ | |||
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+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- LAYOUT SETTINGS --> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <!-- PROMO END --> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <!-- PROJECTS START --> | ||
+ | <div id="vbid-6862ab34-aatxbqmn" class="master item-box gallery-box style-6862ab34-fsvthdyw " data-holder-type="gallery"" data-holder-type="gallery" data-child-type="NATIVE_ORDER" data-styleid="style-6862ab34-fsvthdyw" data-preview-styleid='style-6862ab34-fsvthdyw' data-preset-type-id="PROJECTS"> | ||
+ | <div id="no-image" class="stripe-background load-high-res " ></div> | ||
+ | <div class="gallery-wrapper item-wrapper projects-wrapper "> | ||
+ | |||
+ | <div class="sub container style-6862ab34-fsvthdyw content matrix " data-itemtype="folder" data-creator="" data-itemname="LOADING" data-itemslug="loading" data-itemstyleid="style-6862ab34-fsvthdyw" data-margintop="" data-arranger="matrix" data-layout="multi" data-vbid="vbid-6862ab34-aatxbqmn" data-preset-type-id="PROJECTS" data-preview-style="style-6862ab34-fsvthdyw" data-style="style-8ec4c-t1avbp6mmi" data-absolute-path="/free/amitro02" > | ||
+ | <!-- ARRANGER SETTINGS --> | ||
+ | <div class="arranger-settings" style="display:none;" | ||
+ | data-ARRANGER_TYPE="matrix" | ||
+ | data-ARRANGER_COLS="3" | ||
+ | data-ARRANGER_ITEM_MAX_WIDTH="1000" | ||
+ | data-ARRANGER_ITEM_MIN_WIDTH="219.1" | ||
+ | data-ARRANGER_ITEM_RATIO="1.0" | ||
+ | data-ARRANGER_ITEM_SPACING="15" | ||
+ | data-ARRANGER_ITEMS_PER_PAGE="all" | ||
+ | data-ARRANGER_ORDER_TYPE="regular" | ||
+ | data-AUTO_PLAY="false" | ||
+ | data-AUTO_PLAY_DURATION="4" | ||
+ | data-FLEX_ELEMENT_EFFECT="" | ||
+ | ></div> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
+ | <div id="children"> | ||
+ | |||
+ | |||
+ | <!-- STRIPE HEADER --> | ||
+ | <div class="stripe-header-wrapper"> | ||
+ | <div id="vbid-6862ab34-dz8jy3lh" class="stripe-header sub item-box page-box style-6862ab34-fsvthdyw " data-holder-type="page"" data-holder-type="page" data-child-type="STYLE" data-styleid="style-6862ab34-fsvthdyw" data-preview-styleid='style-6862ab34-fsvthdyw' data-preset-type-id="UNRESOLVED"> | ||
+ | <div class="page-wrapper item-wrapper "> | ||
+ | <div class="item-content leaf blocks_layout page content" data-self="vbid-6862ab34-dz8jy3lh" data-preview-style="style-6862ab34-fsvthdyw" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-6862ab34-dz8jy3lh" data-bgimg=""> | ||
+ | <div class="helper-div middle" > | ||
+ | <!-- <div class="benet" style="min-height:inherit;"></div> --> | ||
+ | <div class="text-side "> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div class="item-details blocks-preview-content-wrapper blocks shrinker-parent" style="position:relative;"> | ||
+ | <div class="blocks-preview-content-holder shrinker-content"> | ||
+ | <!-- BY SPECIFIC ORDER --> | ||
+ | |||
+ | |||
+ | |||
+ | <br class="upper-line-break" /> | ||
+ | <div class="blocks-preview-title-holder removable-parent order-handle"> | ||
+ | |||
+ | <h2 id="vbid-6862ab34-k86zhhid" class="preview-element blocks-preview-title magic-circle-holder inner-page text-element quick-text-style-menu allow-mobile-hide" data-menu-name="BLOCKS_PREVIEW_TITLE" >Plasmid Design and Development</h2> | ||
+ | |||
+ | </div> | ||
+ | <br class="lower-line-break" /> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
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+ | |||
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+ | <p><span style="color: rgb(0, 0, 0);">Plasmid design and development is a key component of the eNose project. Since odorant receptor protein function was tested in a mammalian cell line, the plasmids used to drive expression of these proteins were specifically designed for mammalian cells. For example, the odorant receptor proteins OR10G4 and OR2W1 were expressed using the pCMV6 and pCI plasmids, respectively, and both contain a cytomegalovirus (CMV) promoter region for high gene expression levels in mammalian cells.</span></p> | ||
+ | </div> | ||
+ | |||
</div> | </div> | ||
+ | <br class="lower-line-break" /> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- LAYOUT SETTINGS --> | ||
+ | <div class="layout-settings" style="display:none;" data-type="blocks"></div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="items-holder-wrapper"> | ||
+ | <div id="items-holder"> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div id="vbid-6862ab34-pl59vaem" class="sub item-box page-box style-6862ab34-fsvthdyw " data-holder-type="page"" data-holder-type="page" data-child-type="STYLE" data-styleid="style-6862ab34-fsvthdyw" data-preview-styleid='style-6862ab34-fsvthdyw' data-preset-type-id="UNRESOLVED"> | ||
+ | |||
+ | <div class="page-wrapper item-wrapper "> | ||
+ | |||
+ | <div class="item-content leaf multi_layout page content -container" data-self="vbid-6862ab34-pl59vaem" data-preview-style="style-6862ab34-fsvthdyw" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="1018" data-orig-thumb-width="1086" data-vbid="vbid-6862ab34-pl59vaem" data-bgimg="https://lh3.googleusercontent.com/GmUVauvSM4q1F8T6RGDs41OYsq9bN0V0gNH92lCbNYmijgfqG28kjTgGhwnwqUaMHduQ1e8mgPd4k2AQGA"> | ||
+ | <div class="multi-container preview image-cover" > | ||
+ | <div class="Picture item-preview"> | ||
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+ | <div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style=""> | ||
+ | </div> | ||
+ | <div class="helper-div bottom-center" > | ||
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+ | <div class="pic-side"> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div id="vbid-6862ab34-oxn9qu7b-holder" class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER"> | ||
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+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div> | ||
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+ | |||
+ | <!-- MAP TEMPLATE --> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div> | ||
+ | |||
+ | <!-- RAW TEMPLATE --> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="text-side shrinker-parent"> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div class="item-details preview-content-wrapper multi" style="position:relative;"> | ||
+ | <div class="draggable-div-holder"></div> | ||
+ | <div class="preview-content-holder shrinker-content"> | ||
+ | |||
+ | |||
+ | <!-- BY NATIVE ORDER --> | ||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div> | ||
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+ | |||
+ | |||
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+ | <div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | <div class="preview-form order-handle"> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div> | ||
+ | </div> | ||
+ | |||
+ | <div class="preview-item-links order-handle "> | ||
+ | <div class="preview-links-wrapper"> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- LAYOUT SETTINGS --> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div id="vbid-6862ab34-fhxskqns" class="sub item-box page-box style-6862ab34-fsvthdyw " data-holder-type="page"" data-holder-type="page" data-child-type="STYLE" data-styleid="style-6862ab34-fsvthdyw" data-preview-styleid='style-6862ab34-fsvthdyw' data-preset-type-id="UNRESOLVED"> | ||
+ | |||
+ | <div class="page-wrapper item-wrapper "> | ||
+ | |||
+ | <div class="item-content leaf multi_layout page content -container" data-self="vbid-6862ab34-fhxskqns" data-preview-style="style-6862ab34-fsvthdyw" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="996" data-orig-thumb-width="1049" data-vbid="vbid-6862ab34-fhxskqns" data-bgimg="https://lh3.googleusercontent.com/2wJ8oDa_IKchzZ_15nHnTy2LGDQbWujeavpleuqnx8yqsiQN2nLDCqFzVh-rEikelEObck4g5gSLLh3v2ew"> | ||
+ | <div class="multi-container preview image-cover" > | ||
+ | <div class="Picture item-preview"> | ||
+ | <div class="preview-image-holder"> | ||
+ | <div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style=""> | ||
+ | </div> | ||
+ | <div class="helper-div bottom-center" > | ||
+ | <!-- <div class="benet" style="min-height:inherit;"></div> --> | ||
+ | <div class="pic-side"> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div id="vbid-6862ab34-trna2tpa-holder" class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER"> | ||
+ | |||
+ | <div id="vbid-6862ab34-trna2tpa" class="inner-pic preview-element magic-circle-holder load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/2wJ8oDa_IKchzZ_15nHnTy2LGDQbWujeavpleuqnx8yqsiQN2nLDCqFzVh-rEikelEObck4g5gSLLh3v2ew=s300);" data-orig-width="1049" data-orig-height="996" > | ||
+ | <!-- VIDEO TEMPLATE --> | ||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | <!-- MAP TEMPLATE --> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div> | ||
+ | |||
+ | <!-- RAW TEMPLATE --> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="text-side shrinker-parent"> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div class="item-details preview-content-wrapper multi" style="position:relative;"> | ||
+ | <div class="draggable-div-holder"></div> | ||
+ | <div class="preview-content-holder shrinker-content"> | ||
+ | |||
+ | |||
+ | <!-- BY NATIVE ORDER --> | ||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | <div class="preview-form order-handle"> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div> | ||
+ | </div> | ||
+ | |||
+ | <div class="preview-item-links order-handle "> | ||
+ | <div class="preview-links-wrapper"> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- LAYOUT SETTINGS --> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div id="vbid-6862ab34-pdgrzays" class="sub item-box page-box style-6862ab34-fsvthdyw " data-holder-type="page"" data-holder-type="page" data-child-type="STYLE" data-styleid="style-6862ab34-fsvthdyw" data-preview-styleid='style-6862ab34-fsvthdyw' data-preset-type-id="UNRESOLVED"> | ||
+ | |||
+ | <div class="page-wrapper item-wrapper "> | ||
+ | |||
+ | <div class="item-content leaf multi_layout page content -container" data-self="vbid-6862ab34-pdgrzays" data-preview-style="style-6862ab34-fsvthdyw" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="1191" data-orig-thumb-width="855" data-vbid="vbid-6862ab34-pdgrzays" data-bgimg="https://lh3.googleusercontent.com/oWxTg5xYHS6yJukTKELa5dKxAMZg_sN9VdzA2seb7KYMjw7r_IE5nkH-xMk-hZg1ydVYm7XUNsuCnDP6xkE"> | ||
+ | <div class="multi-container preview image-cover" > | ||
+ | <div class="Picture item-preview"> | ||
+ | <div class="preview-image-holder"> | ||
+ | <div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style=""> | ||
+ | </div> | ||
+ | <div class="helper-div bottom-center" > | ||
+ | <!-- <div class="benet" style="min-height:inherit;"></div> --> | ||
+ | <div class="pic-side"> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div id="vbid-6862ab34-tubzmf7g-holder" class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER"> | ||
+ | |||
+ | <div id="vbid-6862ab34-tubzmf7g" class="inner-pic preview-element magic-circle-holder load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/oWxTg5xYHS6yJukTKELa5dKxAMZg_sN9VdzA2seb7KYMjw7r_IE5nkH-xMk-hZg1ydVYm7XUNsuCnDP6xkE=s300);" data-orig-width="855" data-orig-height="1191" > | ||
+ | <!-- VIDEO TEMPLATE --> | ||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | <!-- MAP TEMPLATE --> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div> | ||
+ | |||
+ | <!-- RAW TEMPLATE --> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="text-side shrinker-parent"> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div class="item-details preview-content-wrapper multi" style="position:relative;"> | ||
+ | <div class="draggable-div-holder"></div> | ||
+ | <div class="preview-content-holder shrinker-content"> | ||
+ | |||
+ | |||
+ | <!-- BY NATIVE ORDER --> | ||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | <div class="preview-form order-handle"> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div> | ||
+ | </div> | ||
+ | |||
+ | <div class="preview-item-links order-handle "> | ||
+ | <div class="preview-links-wrapper"> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- LAYOUT SETTINGS --> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <!-- PROJECTS END --> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <!-- PROJECTS START --> | ||
+ | <div id="vbid-2a27a910-ffov8ogy" class="master item-box gallery-box style-2a27a910-2asblupt " data-holder-type="gallery"" data-holder-type="gallery" data-child-type="NATIVE_ORDER" data-styleid="style-2a27a910-2asblupt" data-preview-styleid='style-2a27a910-2asblupt' data-preset-type-id="PROJECTS"> | ||
+ | <div id="no-image" class="stripe-background load-high-res " ></div> | ||
+ | <div class="gallery-wrapper item-wrapper projects-wrapper "> | ||
+ | |||
+ | <div class="sub container style-2a27a910-2asblupt content matrix " data-itemtype="folder" data-creator="" data-itemname="LOADING" data-itemslug="loading" data-itemstyleid="style-2a27a910-2asblupt" data-margintop="" data-arranger="matrix" data-layout="multi" data-vbid="vbid-2a27a910-ffov8ogy" data-preset-type-id="PROJECTS" data-preview-style="style-2a27a910-2asblupt" data-style="style-8ec4c-t1avbp6mmi" data-absolute-path="/free/amitro02" > | ||
+ | <!-- ARRANGER SETTINGS --> | ||
+ | <div class="arranger-settings" style="display:none;" | ||
+ | data-ARRANGER_TYPE="matrix" | ||
+ | data-ARRANGER_COLS="3" | ||
+ | data-ARRANGER_ITEM_MAX_WIDTH="1000" | ||
+ | data-ARRANGER_ITEM_MIN_WIDTH="338.8" | ||
+ | data-ARRANGER_ITEM_RATIO="1.0" | ||
+ | data-ARRANGER_ITEM_SPACING="15" | ||
+ | data-ARRANGER_ITEMS_PER_PAGE="all" | ||
+ | data-ARRANGER_ORDER_TYPE="regular" | ||
+ | data-AUTO_PLAY="false" | ||
+ | data-AUTO_PLAY_DURATION="4" | ||
+ | data-FLEX_ELEMENT_EFFECT="" | ||
+ | ></div> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
+ | <div id="children"> | ||
+ | |||
+ | |||
+ | <!-- STRIPE HEADER --> | ||
+ | <div class="stripe-header-wrapper"> | ||
+ | <div id="vbid-2a27a910-gg22i4be" class="stripe-header sub item-box page-box style-2a27a910-2asblupt " data-holder-type="page"" data-holder-type="page" data-child-type="STYLE" data-styleid="style-2a27a910-2asblupt" data-preview-styleid='style-2a27a910-2asblupt' data-preset-type-id="UNRESOLVED"> | ||
+ | <div class="page-wrapper item-wrapper "> | ||
+ | <div class="item-content leaf blocks_layout page content" data-self="vbid-2a27a910-gg22i4be" data-preview-style="style-2a27a910-2asblupt" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="" data-orig-thumb-width="" data-vbid="vbid-2a27a910-gg22i4be" data-bgimg=""> | ||
+ | <div class="helper-div middle" > | ||
+ | <!-- <div class="benet" style="min-height:inherit;"></div> --> | ||
+ | <div class="text-side "> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div class="item-details blocks-preview-content-wrapper blocks shrinker-parent" style="position:relative;"> | ||
+ | <div class="blocks-preview-content-holder shrinker-content"> | ||
+ | <!-- BY SPECIFIC ORDER --> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <br class="upper-line-break" /> | ||
+ | <div class="blocks-preview-body-holder removable-parent order-handle"> | ||
+ | |||
+ | <div id="vbid-2a27a910-q8tctoi7" class="preview-element blocks-preview-body magic-circle-holder text-element quick-text-style-menu allow-mobile-hide" data-menu-name="BLOCKS_PREVIEW_BODY"> | ||
+ | <p class="font_8"><span style="color: rgb(0, 0, 0);">Shown are figures of the PCR that amplified the coding sequences for OR2W1 and OR10G4, and images of gels for the restriction enzyme digests confirming the production of the OR10G4 in pSB1C3 and OR2W1 in pSB1C3 deliverables.</span></p><p class="font_8"><span style="color: rgb(0, 0, 0);"><span class="wixGuard"></span></span></p><p class="font_8"><span style="color: rgb(0, 0, 0);">For the PCR reactions, the expected bands for the OR10G4 and OR2W1 are both about 0.95kb (above).</span></p><p class="font_8"><span style="color: rgb(0, 0, 0);"><span class="wixGuard"></span></span></p><p class="font_8"><span style="color: rgb(0, 0, 0);">For the OR10G4 restriction enzyme digest reactions, the expected products are (Below): 2.3kb and 0.7kb for the EcoRI and BamHI reaction, 2.3kb and 0.7kb for the BlpI and PstI reaction.</span></p><p class="font_8"><br></p><p class="font_8"><span style="color: rgb(0, 0, 0);">For the OR2W1 restriction enzyme digest reactions, the expected products are (left):2.2kb and 0.8kb for the BglII and PstI reaction, 1.8kb and 1.2kb for the MscI reaction.</span></p><p class="font_8"><span style="color: rgb(0, 0, 0);"><span class="wixGuard"></span></span></p><p class="font_8"><span style="color: rgb(0, 0, 0);">Additionally, since olfactory receptors are from the same family of proteins, other sequences were examined under restriction enzyme digest but not inserted into the pSB1C3 plasmid (below).</span></p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <br class="lower-line-break" /> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- LAYOUT SETTINGS --> | ||
+ | <div class="layout-settings" style="display:none;" data-type="blocks"></div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="items-holder-wrapper"> | ||
+ | <div id="items-holder"> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div id="vbid-2a27a910-oal9bp0r" class="sub item-box page-box style-2a27a910-2asblupt " data-holder-type="page"" data-holder-type="page" data-child-type="STYLE" data-styleid="style-2a27a910-2asblupt" data-preview-styleid='style-2a27a910-2asblupt' data-preset-type-id="UNRESOLVED"> | ||
+ | |||
+ | <div class="page-wrapper item-wrapper "> | ||
+ | |||
+ | <div class="item-content leaf multi_layout page content -container" data-self="vbid-2a27a910-oal9bp0r" data-preview-style="style-2a27a910-2asblupt" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="1742" data-orig-thumb-width="867" data-vbid="vbid-2a27a910-oal9bp0r" data-bgimg="https://lh3.googleusercontent.com/HRE7sHYap4xcQtL-Y17xARv4vBTZ44y_TIZgL95YbWUYA52NCJsBzgmEPce6VupZdS-dobW1AAD9gRG-EQ"> | ||
+ | <div class="multi-container preview image-cover" > | ||
+ | <div class="Picture item-preview"> | ||
+ | <div class="preview-image-holder"> | ||
+ | <div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style=""> | ||
+ | </div> | ||
+ | <div class="helper-div bottom-center" > | ||
+ | <!-- <div class="benet" style="min-height:inherit;"></div> --> | ||
+ | <div class="pic-side"> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div id="vbid-2a27a910-piwxyxqa-holder" class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER"> | ||
+ | |||
+ | <div id="vbid-2a27a910-piwxyxqa" class="inner-pic preview-element magic-circle-holder load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/HRE7sHYap4xcQtL-Y17xARv4vBTZ44y_TIZgL95YbWUYA52NCJsBzgmEPce6VupZdS-dobW1AAD9gRG-EQ=s300);" data-orig-width="867" data-orig-height="1742" > | ||
+ | <!-- VIDEO TEMPLATE --> | ||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | <!-- MAP TEMPLATE --> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div> | ||
+ | |||
+ | <!-- RAW TEMPLATE --> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="text-side shrinker-parent"> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div class="item-details preview-content-wrapper multi" style="position:relative;"> | ||
+ | <div class="draggable-div-holder"></div> | ||
+ | <div class="preview-content-holder shrinker-content"> | ||
+ | |||
+ | |||
+ | <!-- BY NATIVE ORDER --> | ||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | <div class="preview-form order-handle"> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div> | ||
+ | </div> | ||
+ | |||
+ | <div class="preview-item-links order-handle "> | ||
+ | <div class="preview-links-wrapper"> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='SOCIAL' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- LAYOUT SETTINGS --> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div id="vbid-2a27a910-y70abt8o" class="sub item-box page-box style-2a27a910-2asblupt " data-holder-type="page"" data-holder-type="page" data-child-type="STYLE" data-styleid="style-2a27a910-2asblupt" data-preview-styleid='style-2a27a910-2asblupt' data-preset-type-id="UNRESOLVED"> | ||
+ | |||
+ | <div class="page-wrapper item-wrapper "> | ||
+ | |||
+ | <div class="item-content leaf multi_layout page content -container" data-self="vbid-2a27a910-y70abt8o" data-preview-style="style-2a27a910-2asblupt" data-style="style-8ec4c-ed91kjv1sl" data-orig-thumb-height="1753" data-orig-thumb-width="895" data-vbid="vbid-2a27a910-y70abt8o" data-bgimg="https://lh3.googleusercontent.com/602Ea2D1TPGR-PXfdK57eQJ-UDbQ3UKINAMtsB-GwQbVNp6jF46aKnUyvGCniIvJeAJybHGx111ofMBe5o0"> | ||
+ | <div class="multi-container preview image-cover" > | ||
+ | <div class="Picture item-preview"> | ||
+ | <div class="preview-image-holder"> | ||
+ | <div id="no-image" class="background-image-div preview-element image-source magic-circle-holder unfold-left load-high-res" data-menu-name="BACKGROUND_IMAGE" style=""> | ||
+ | </div> | ||
+ | <div class="helper-div bottom-center" > | ||
+ | <!-- <div class="benet" style="min-height:inherit;"></div> --> | ||
+ | <div class="pic-side"> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div id="vbid-2a27a910-ccibivql-holder" class="preview-image-holder inner-pic-holder" data-menu-name="PREVIEW_INLINE_IMAGE_HOLDER"> | ||
+ | |||
+ | <div id="vbid-2a27a910-ccibivql" class="inner-pic preview-element magic-circle-holder load-high-res " data-menu-name="PREVIEW_INLINE_IMAGE" style="background-image:url(https://lh3.googleusercontent.com/602Ea2D1TPGR-PXfdK57eQJ-UDbQ3UKINAMtsB-GwQbVNp6jF46aKnUyvGCniIvJeAJybHGx111ofMBe5o0=s300);" data-orig-width="895" data-orig-height="1753" > | ||
+ | <!-- VIDEO TEMPLATE --> | ||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='VIDEO' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | <!-- MAP TEMPLATE --> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div> | ||
+ | |||
+ | <!-- RAW TEMPLATE --> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <div class="text-side shrinker-parent"> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div class="item-details preview-content-wrapper multi" style="position:relative;"> | ||
+ | <div class="draggable-div-holder"></div> | ||
+ | <div class="preview-content-holder shrinker-content"> | ||
+ | |||
+ | |||
+ | <!-- BY NATIVE ORDER --> | ||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='ICON' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='TITLE' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='SUBTITLE' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='DIVIDER' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='BODY' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | |||
+ | <div class="element-placeholder" data-elementtype='PRICE' style="display:none;"></div> | ||
+ | |||
+ | |||
+ | <div class="preview-form order-handle"> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='FIELD' style="display:none;"></div> | ||
+ | </div> | ||
+ | |||
+ | <div class="preview-item-links order-handle "> | ||
+ | <div class="preview-links-wrapper"> | ||
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+ | <div class="element-placeholder" data-elementtype='LINK' style="display:none;"></div> | ||
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+ | </div> | ||
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+ | |||
+ | |||
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+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- LAYOUT SETTINGS --> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <!-- PROJECTS END --> | ||
+ | |||
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+ | |||
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+ | <div id="no-image" class="stripe-background load-high-res " ></div> | ||
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+ | |||
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+ | data-ARRANGER_ITEM_MIN_WIDTH="230" | ||
+ | data-ARRANGER_ITEM_RATIO="1" | ||
+ | data-ARRANGER_ITEM_SPACING="20" | ||
+ | data-ARRANGER_ITEMS_PER_PAGE="all" | ||
+ | data-ARRANGER_ORDER_TYPE="regular" | ||
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+ | <p class="font_8" style="line-height: 1.8em;line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);">For construction of iGEM deliverables containing odorant receptor proteins in the pSB1C3 plasmid, the coding sequences for OR10G4 and OR2W1 were amplified by PCR using the respective pCMV6 and pCI mammalian expression plasmids as templates. The PCR reactions were expected to generate bands of approximately 0.95kb for both OR10G4 and OR2W1 coding sequences, and the image of the gel for the PCR products shows bands at this expected size. These PCR products were amplified using primers that incorporated EcoRI and PstI restriction endonuclease sites at the 5’ and 3’ ends of the PCR products. Digesting both the PCR products and the pre-linearized pSB1C3 plasmid supplied by iGEM with EcoRI and PstI restriction endonucleases, the PCR products were ligated into the pSB1C3 plasmid. The ligation reaction was used to transform chemically competent DH5alpha cells. To screen for colonies containing the appropriately inserted odorant receptor gene and pSB1C3 plasmid, colonies were used to inoculate small cultures. The plasmids were harvested from the cultures by mini-prep plasmid isolation kits, and the isolated plasmids were digested with two sets of restriction endonuclease reactions. </span></p> | ||
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+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
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+ | <div id="no-image" class="stripe-background load-high-res " ></div> | ||
+ | <div class="gallery-wrapper item-wrapper text_block-wrapper "> | ||
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+ | <!-- ARRANGER SETTINGS --> | ||
+ | <div class="arranger-settings" style="display:none;" | ||
+ | data-ARRANGER_TYPE="flex" | ||
+ | data-ARRANGER_COLS="0" | ||
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+ | data-ARRANGER_ITEMS_PER_PAGE="all" | ||
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+ | data-AUTO_PLAY="true" | ||
+ | data-AUTO_PLAY_DURATION="4" | ||
+ | data-FLEX_ELEMENT_EFFECT="effect-fadein" | ||
+ | ></div> | ||
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+ | <div class="helper-div middle-center" > | ||
+ | <!-- <div class="benet" style="min-height:inherit;"></div> --> | ||
+ | <div class="pic-side"> | ||
+ | <div class="vertical-aligner"> | ||
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+ | <!-- VIDEO TEMPLATE --> | ||
+ | |||
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+ | |||
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+ | |||
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+ | |||
+ | |||
+ | </div> | ||
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+ | <div class="text-side shrinker-parent"> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div class="item-details preview-content-wrapper multi" style="position:relative;"> | ||
+ | <div class="draggable-div-holder"></div> | ||
+ | <div class="preview-content-holder shrinker-content"> | ||
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+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
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+ | <!-- LAYOUT SETTINGS --> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
+ | |||
+ | </div> | ||
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+ | |||
+ | |||
+ | |||
+ | |||
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+ | |||
+ | |||
+ | |||
+ | |||
+ | <!-- TEXT_BLOCK START --> | ||
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+ | <div id="no-image" class="stripe-background load-high-res " ></div> | ||
+ | <div class="gallery-wrapper item-wrapper text_block-wrapper "> | ||
+ | |||
+ | <div class="sub container style-79d95830-hbfm8aer content flex " data-itemtype="folder" data-creator="" data-itemname="LOADING Copy" data-itemslug="loading-copy" data-itemstyleid="style-79d95830-hbfm8aer" data-margintop="" data-arranger="flex" data-layout="multi" data-vbid="vbid-79d95830-cmzxvy05" data-preset-type-id="TEXT_BLOCK" data-preview-style="style-79d95830-hbfm8aer" data-style="style-4e411-j0xhjgzynh" data-absolute-path="/free/amitro02" > | ||
+ | <!-- ARRANGER SETTINGS --> | ||
+ | <div class="arranger-settings" style="display:none;" | ||
+ | data-ARRANGER_TYPE="flex" | ||
+ | data-ARRANGER_COLS="0" | ||
+ | data-ARRANGER_ITEM_MAX_WIDTH="400" | ||
+ | data-ARRANGER_ITEM_MIN_WIDTH="230" | ||
+ | data-ARRANGER_ITEM_RATIO="1" | ||
+ | data-ARRANGER_ITEM_SPACING="20" | ||
+ | data-ARRANGER_ITEMS_PER_PAGE="all" | ||
+ | data-ARRANGER_ORDER_TYPE="regular" | ||
+ | data-AUTO_PLAY="true" | ||
+ | data-AUTO_PLAY_DURATION="4" | ||
+ | data-FLEX_ELEMENT_EFFECT="effect-fadein" | ||
+ | ></div> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
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+ | <div class="helper-div middle-left" > | ||
+ | <!-- <div class="benet" style="min-height:inherit;"></div> --> | ||
+ | <div class="pic-side"> | ||
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+ | <!-- VIDEO TEMPLATE --> | ||
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+ | |||
+ | <!-- MAP TEMPLATE --> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='MAP' style="display:none;"></div> | ||
+ | |||
+ | <!-- RAW TEMPLATE --> | ||
+ | |||
+ | <div class="element-placeholder" data-elementtype='RAW' style="display:none;"></div> | ||
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+ | |||
+ | |||
+ | </div> | ||
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+ | <div class="text-side shrinker-parent"> | ||
+ | <div class="vertical-aligner"> | ||
+ | <div class="item-details preview-content-wrapper multi" style="position:relative;"> | ||
+ | <div class="draggable-div-holder"></div> | ||
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+ | <!-- BY SPECIFIC ORDER --> | ||
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+ | |||
+ | |||
+ | |||
+ | <br class="upper-line-break" /> | ||
+ | <div class="preview-title-holder removable-parent order-handle"> | ||
+ | |||
+ | <h2 id="element-a2ede40f1f1fdb0" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Animal Cell Nucleofection</h2> | ||
+ | |||
+ | </div> | ||
+ | <br class="lower-line-break" /> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <br class="upper-line-break" /> | ||
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+ | <p class="font_8" style="line-height: 1.8em;line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);">Transferring the olfactory receptor into animal cells is critical component to the development of a working biosensor. However, determining the production of the olfactory receptor was determined by three methods: 1) Western Blotting to determine if the olfactory receptor protein was produced, 2) immunohistochemistry ensure the receptor was transported to the membrane, and 3) physiological response of cell action potentials in the presence of specific odorants.</span><br></p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <br class="lower-line-break" /> | ||
+ | |||
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+ | |||
+ | |||
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+ | |||
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+ | |||
+ | |||
+ | </div> | ||
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+ | </div> | ||
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+ | </div> | ||
+ | </div> | ||
+ | <!-- LAYOUT SETTINGS --> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
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+ | |||
+ | |||
+ | |||
+ | |||
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+ | <p class="font_8" style="line-height: 1.8em;line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);">Sample ORs were selected and analyzed via Western Blotting to determine the size and purity of the antigen protein. The image below is a Western Blot of HT-22 Cells. From left to right: Ladder, Ladder, Control, GPF, OR5P3, OR5A1, OR51E1, OR02C1 OR10G4, and OR2W1. The green bands at ~37 kDa prove that OR proteins were produced after 3 days in culture meaning that primary and secondary antibodies matched and bound to target OR antigens.</span><br></p> | ||
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+ | |||
+ | </div> | ||
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+ | <!-- BY SPECIFIC ORDER --> | ||
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+ | |||
+ | |||
+ | |||
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+ | |||
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+ | |||
+ | |||
+ | |||
+ | |||
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+ | <p class="font_8" style="line-height: 1.8em;line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);">OR transfected cells were also captured, displayed and analyzed via fluorescence and confocal microscopy. Primary immunohistochemistry exploits a target OR antigen, while secondary immunohistochemistry proves the initial attachment of the primary antibody. The images below show both fluorescence (top) and confocal (bottom) microscopy.</span><br></p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
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+ | <!-- LAYOUT SETTINGS --> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
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+ | <h2 id="vbid-7e3671b9-qh1fhrsb" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Physiological Response<br></h2> | ||
+ | |||
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+ | |||
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+ | |||
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+ | <p class="font_8" style="line-height: 1.8em;line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);">HT-22 cells were cultured on an Axion Biosystems Microelectrode Array cell culture dish to measure field potential measurements of depolarization. HT-22 cells nucleofected with OR 2W1 were presented with hexanol, a positive ligand for the OR 2W1 receptor. Multiple additions were made in 1 minute increments revealing the resetting capability and max depolarization of the HT-22 cells.</span><br></p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <br class="lower-line-break" /> | ||
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+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
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+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
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+ | |||
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+ | |||
+ | |||
+ | <br class="upper-line-break" /> | ||
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+ | |||
+ | <h2 id="vbid-d3d8cab0-la0t59sc" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Handheld Photosensor Device<br></h2> | ||
+ | |||
+ | </div> | ||
+ | <br class="lower-line-break" /> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <br class="upper-line-break" /> | ||
+ | <div class="preview-body-holder removable-parent order-handle"> | ||
+ | |||
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+ | <p class="font_8" style="line-height: 1.8em;line-height: 1.8em; font-size: 15px; text-align: left;"><span style="color: rgb(0, 0, 0);">Two prototypes have been designed and developed capable of utilizing different methods of biosensor detection. One of the devices utilizes a photo sensor connected to an Arduino, translating the cell action potentials into plain text. </span><br></p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <br class="lower-line-break" /> | ||
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+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
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+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
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+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
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+ | <!-- ARRANGER SETTINGS --> | ||
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+ | ></div> | ||
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+ | |||
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+ | |||
+ | |||
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+ | |||
+ | <h2 id="vbid-b5ab922d-ft2uybjm" class="preview-element preview-title magic-circle-holder inner-page text-element quick-text-style-menu allow-mobile-hide" data-menu-name="PREVIEW_TITLE" >Microelectrode Array Bioreactor<br></h2> | ||
+ | |||
+ | </div> | ||
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+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
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+ | <p class="font_8" style="line-height: 1.5em;"><span style="color: rgb(0, 0, 0);">In a controlled environment, neurons with enhanced olfactory receptors are cultured on a microelectrode array. While media is flowed through via a peristaltic pump, certain olfactants are injected. When the neurons detect these olfactants, they produce an electrical signal, which is then transferred to a computer for interpretation.</span></p><p class="font_8" style="line-height: 1.5em;"><span style="color: rgb(0, 0, 0);"><span class="wixGuard"></span></span></p><p class="font_8" style="line-height: 1.5em;"><span style="color: rgb(0, 0, 0);">After conducting multiple trials, it was shown that there was no significant increase in voltage reading the olfactant was introduced into the closed system. This may have been because the plasmid is not being expressed sufficiently, or that 24 hours after transfection was not the optimal time to conduct trials. There was also no reference electrode, which could have altered the computers interpretation of the voltage data</span><br></p> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <br class="lower-line-break" /> | ||
+ | |||
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+ | |||
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+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- LAYOUT SETTINGS --> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
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+ | |||
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+ | <!-- ARRANGER SETTINGS --> | ||
+ | <div class="arranger-settings" style="display:none;" | ||
+ | data-ARRANGER_TYPE="flex" | ||
+ | data-ARRANGER_COLS="0" | ||
+ | data-ARRANGER_ITEM_MAX_WIDTH="400" | ||
+ | data-ARRANGER_ITEM_MIN_WIDTH="230" | ||
+ | data-ARRANGER_ITEM_RATIO="1" | ||
+ | data-ARRANGER_ITEM_SPACING="20" | ||
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+ | data-AUTO_PLAY="true" | ||
+ | data-AUTO_PLAY_DURATION="4" | ||
+ | data-FLEX_ELEMENT_EFFECT="effect-fadein" | ||
+ | ></div> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
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+ | |||
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+ | <div class="text-side shrinker-parent"> | ||
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+ | <div class="draggable-div-holder"></div> | ||
+ | <div class="preview-content-holder shrinker-content"> | ||
+ | |||
+ | <!-- BY SPECIFIC ORDER --> | ||
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+ | |||
+ | |||
+ | <br class="upper-line-break" /> | ||
+ | <div class="preview-title-holder removable-parent order-handle"> | ||
+ | |||
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+ | |||
+ | </div> | ||
+ | <br class="lower-line-break" /> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <br class="upper-line-break" /> | ||
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+ | |||
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+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <div class="preview-item-links order-handle removable-parent" style="display:inline-block;"> | ||
+ | |||
+ | <span id="vbid-7d38c6a1-ynlsfdhm" class="preview-element Link item-link magic-circle-holder text-element custom" data-menu-name="PREVIEW_LINK" >TEL: 845.938.3915</span> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <br class="upper-line-break" /> | ||
+ | <div class="preview-social-wrapper removable-parent order-handle" > | ||
+ | <div id="vbid-7d38c6a1-bdvlps7u" class="preview-element preview-social-holder magic-circle-holder" data-menu-name="PREVIEW_SOCIAL" data-theme="8" > | ||
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+ | <a class='social-link-url' href="https://www.facebook.com/West-Point-Department-of-Chemistry-and-Life-Science-385389195808/" target='_blank'><img class='preview-link-img ' src="//www.imcreator.com/images/socialmedia/8facebook.png" ></a> | ||
+ | </div> | ||
+ | |||
+ | <div id="TWITTER" class='link-entry ' data-menu-name="PREVIEW_SOCIAL" style='display:inline-block;' data-title="TWITTER" data-img-url="//www.imcreator.com/images/socialmedia/8twitter.png"> | ||
+ | <a class='social-link-url' href="https://twitter.com/WestPoint_USMA?ref_src=twsrc%5Egoogle%7Ctwcamp%5Eserp%7Ctwgr%5Eauthor" target='_blank'><img class='preview-link-img ' src="//www.imcreator.com/images/socialmedia/8twitter.png" ></a> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
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+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- LAYOUT SETTINGS --> | ||
+ | <div class="layout-settings" style="display:none;" data-type="multi"></div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <!-- FOOTERS END --> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
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+ | <div class="light-box-image-holder"> | ||
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+ | <script src="//www.imcreator.com/js/lightbox.js?v=1.4.1f" type="text/javascript"></script> | ||
+ | <script src="//www.imcreator.com/js/spimeengine.js?v=1.4.1f" type="text/javascript"></script> | ||
+ | |||
+ | |||
+ | <!-- SHOPROCKET ECOMMERCE --> | ||
+ | <script> | ||
+ | var JSR = jQuery.noConflict(); | ||
+ | var $ = jQuery.noConflict(); | ||
+ | </script> | ||
+ | |||
+ | <input type="hidden" name="sr-companyid" id="sr-companyid" value=""> | ||
+ | </body> | ||
+ | |||
</html> | </html> |
Revision as of 04:41, 1 November 2017
USMA-West_Point
<!DOCTYPE html>