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| − | <h2>Wiki of InterLab by UST_Beijing</h2><br>
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| − | <div class="panel panel-transparent">
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| − | <div class="panel-heading" role="tab">
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| − | <h4 class="panel-title"><h3>Background</h3></h4>
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| − | <div id="Background" class="panel-collapse collapse in">
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| − | <div class="panel-body">
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| − | <p class="text-justify" align="left">
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| − | “All of the 2017 iGEM teams are invited and encouraged to participate in the Fourth International InterLaboratory Measurement Study in synthetic biology.” The goal of our team is to use the InterLab protocol and Measurement Kit provided by iGEM official to do measurements which can help to establish a GFP measurement protocol based on engineering principles that anyone with a plate reader can use in their lab. The specific task for us is to measure GFP fluorescence and standard curve by using plate reader.
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| − | <div class="panel-heading">
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| − | <h4 class="panel-title"><h3>Materials & Methods</h3></h4>
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| − | </div>
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| − | <div id="Abstract" class="panel-collapse collapse in">
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| − | <h4 style="border-bottom:1px solid black; padding-top:6px;padding-bottom: 12px">Plasmids used</h4>
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| − | <p class="text-justify" style="text-align:center" >
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| − | Kit Plate 7 <br/>
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| − | Positive Control (BBa_I20270): well 21B<br/>
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| − | Negative Control (BBa_R0040): well 21D<br/>
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| − | Test Device 1 (BBa_J364000): well 21F<br/>
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| − | Test Device 2 (BBa_J364001): well 21H<br/>
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| − | Test Device 3 (BBa_J364002): well 21J<br/>
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| − | Test Device 4 (BBa_J364003): well 21L<br/>
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| − | Test Device 5 (BBa_J364004): well 21N<br/>
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| − | Test Device 6 (BBa_J364005): well 21P<br/>
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| − | <br/>
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| | </p> | | </p> |
| | <h4 style="border-bottom:1px solid black; padding-top:20px;padding-bottom: 12px">Strain used</h4> | | <h4 style="border-bottom:1px solid black; padding-top:20px;padding-bottom: 12px">Strain used</h4> |
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| − | <h4 style="border-bottom:1px solid black; padding-top:20px;padding-bottom: 12px">Materials</h4>
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| − | FITC Standard: one tube with dried down FITC for creating a FITC standard <br/>
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| − | • LUDOX: one tube with 30% colloidal silica suspended in 1mL of water <br/>
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| − | • 1xPBS (phosphate buffered saline) <br/>
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| − | • LB (Luria Bertani) media <br/>
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| − | • Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH) <br/>
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| − | • 50 ml Falcon tube (or equivalent) or 250 ml shake flask for cell growth <br/>
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| − | • 1.5 ml eppendorf tubes for sample storage <br/>
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| − | • Ice bucket with ice <br/>
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| − | • Pipettes <br/>
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| − | • 96 well plate <br/>
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| | </p> | | </p> |
| − | <h4 style="border-bottom:1px solid black; padding-top:20px;padding-bottom: 12px">Machines</h4>
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| − | <p class="text-justify" style="text-align:center" >
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| − | Enspire 2300-001M<br/>
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| − | Sigma Laborzentrifugen<br/>
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| − | Thermo Scientific Nanodrop 2000<br/>
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| − | EVOS AMG microscope<br/>
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| − | </p>
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| − | <h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Software</h4>
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| − | <p class="text-justify" style="text-align:center" >
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| − | Microsoft Excel<br/>
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| − | </p>
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| − | <h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Methods</h4>
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| − | <p class="text-justify" style="text-align:center" >
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| − | Calibration<br/>
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| − | OD600 Reference point<br/>
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| − | FITC fluorescence standard curve<br/>
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| − | Test Device 1 (BBa_J364000): well 21F<br/>
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| − | Test Device 2 (BBa_J364001): well 21H<br/>
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| − | Test Device 3 (BBa_J364002): well 21J<br/>
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| − | Test Device 4 (BBa_J364003): well 21L<br/>
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| − | Test Device 5 (BBa_J364004): well 21N<br/>
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| − | Test Device 6 (BBa_J364005): well 21P<br/>
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| − | </p>
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| | <h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Cell measurement</h4> | | <h4 style="border-bottom:1px solid black; padding-top: 20px;padding-bottom: 12px">Cell measurement</h4> |
