Difference between revisions of "Team:UrbanTundra Edmonton/InterLab"
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<h2>Background</h2> | <h2>Background</h2> | ||
<p>This year, our team participated in the fourth InterLab Measurement Study which aimed to establish and test a standardized protocol to measure GFP that can produce common and consistent numbers from all across the world. Doing our part, we used a plate reader, which we were given access to from the UAlberta iGEM team along with support from members of the UAlberta iGEM team to measure GFP fluorescence. Our team followed the protocols outlined in the iGEM InterLab page (link).</p> | <p>This year, our team participated in the fourth InterLab Measurement Study which aimed to establish and test a standardized protocol to measure GFP that can produce common and consistent numbers from all across the world. Doing our part, we used a plate reader, which we were given access to from the UAlberta iGEM team along with support from members of the UAlberta iGEM team to measure GFP fluorescence. Our team followed the protocols outlined in the iGEM InterLab page (link).</p> | ||
| − | <h2>Materials | + | <h2>Materials</h2> |
<p><b>Plasmids used:</b><br>Devices (from InterLab Measurement Kit):<br>Positive control<br>Negative control<br>Test Device 1: J23101+I13504<br>Test Device 2: J23106+I13504<br>Test Device 3: J23117+I13504<br>Test Device 4: J23101.BCD2.E0040.B0015<br>Test Device 5: J23106.BCD2.E0040.B0015<br>Test Device 6: J23117.BCD2.E0040.B0015</p> | <p><b>Plasmids used:</b><br>Devices (from InterLab Measurement Kit):<br>Positive control<br>Negative control<br>Test Device 1: J23101+I13504<br>Test Device 2: J23106+I13504<br>Test Device 3: J23117+I13504<br>Test Device 4: J23101.BCD2.E0040.B0015<br>Test Device 5: J23106.BCD2.E0040.B0015<br>Test Device 6: J23117.BCD2.E0040.B0015</p> | ||
| − | + | <p><b>Strain used:</b><br>Competent cells (Escherichia coli strain DH5α)</p> | |
</div> | </div> | ||
</div> | </div> | ||
Revision as of 05:26, 1 November 2017
InterLab
Background
This year, our team participated in the fourth InterLab Measurement Study which aimed to establish and test a standardized protocol to measure GFP that can produce common and consistent numbers from all across the world. Doing our part, we used a plate reader, which we were given access to from the UAlberta iGEM team along with support from members of the UAlberta iGEM team to measure GFP fluorescence. Our team followed the protocols outlined in the iGEM InterLab page (link).
Materials
Plasmids used:
Devices (from InterLab Measurement Kit):
Positive control
Negative control
Test Device 1: J23101+I13504
Test Device 2: J23106+I13504
Test Device 3: J23117+I13504
Test Device 4: J23101.BCD2.E0040.B0015
Test Device 5: J23106.BCD2.E0040.B0015
Test Device 6: J23117.BCD2.E0040.B0015
Strain used:
Competent cells (Escherichia coli strain DH5α)
