Difference between revisions of "Team:Valencia UPV"

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PlantSynBio
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<h2 class="page-title" style="margin-top: 50px; margin-bottom: 30px; color:#cd545b">Project Description</h2>
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<p align="justify" style= "text-indent:30px; color:#005368">Ensuring regular access to high-quality food for everyone is one of the mainstays of society. Unfortunately, food security is constantly threatened by many unpredictable factors that involve agriculture. Controlling both development and behaviour of the plant could contribute to make crops more productive and sustainable. We, Valencia UPV team, propose <b>ChatterPlant</b>, a SynBio-based solution to communicate with plants. We designed an optogenetic circuit that works as interface between plants and humans. Bearing that in mind, we engineered <b>ChatterBox</b> that regulates precisely plant growth conditions while translating plant information into an intelligible one.</p>
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<h1 style="font-weight: 500 !important;"><span>ABSTRACT</span></h1>
<p align="justify" style= "text-indent:30px; color:#005368">Plant’s communication ability relies on the plant-human <b>interface</b> comprised by our designed optogenetic circuit. This <b>modular system</b> is triggered by red/far-red light inducible switch, thus allowing users to control expression of any desired pathway easily.  Since white light in aerial parts could interfere with the circuit signalling, these light stimuli would be introduced by illuminating plant’s roots. Furthermore, circuit's modularity allows to cover almost any users' necessities, personalizing the final element in the system. As a proof of concept, we resolve to regulate flowering periods in order to provide more control over crops. Thus, we intend to make a positive impact in global <b>food security</b> progress. However, we do not only intend to give orders to plants but also to acquire relevant information from them. Therefore, we generated a second communication channel that allows plants to send us information about their current status (i.g. metabolites levels, phytohormones levels, pathogen presence…). In our project, we chose to activate the expression of a fluorescent protein when infection occurs, making it easily detectable.</p>
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<p align="justify" style="text-indent:30px; margin-bottom: 50px; color:#005368">In order to achieve that control, we developed <b>ChatterBox</b> with the aim of translating our orders into light stimuli through roots and also tracking plant growth and development. Controlling external factors and regulating plant’s features allow us to increase plant versatility and adaptative capacity. Furthermore, a <b>detection system</b>  is permanently checking for stress signals, so we could immediately actuate in consequence. With this project, our team intends to help in developing a <b>non-climate dependent agriculture</b>, as well as in giving farmers more <b>control</b> over their crops. Thus,  we aspire to ensure food security through human-plant interaction.</p>
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<p class="jopesangria">Urban overpopulation, climate change and natural resources decrement are threatening <strong class="jopenegrita">food security</strong>. Ensuring season-less, accessible and local food production promotes a sustainable agriculture. Valencia_UPV provides a whole new system to <strong class="jopenegrita">control plant physiology</strong> at both genetic and environmental level.</p>
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<p class="jopesangria">ChatterPlant is a SynBio-based solution that works as plant-human interface allowing a <strong class="jopenegrita">bidirectional communication</strong>. First, a root-specific modular <strong class="jopenegrita">optogenetic</strong> circuit enables control on plants´ endogenous gene expression (e.g flowering). Then, a <strong class="jopenegrita">sensor</strong> circuit with color coded output provides specific information of stress conditions, accelerating corrective measures. The genetic setup is complemented with a <strong class="jopenegrita">hardware device</strong>, ChatterBox, specially designed to control plant’s growth conditions.</p>
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<p class="jopesangria">ChatterPlant’s possibilities can be improved gathering Plant SynBio knowledge. PlantLabCo is an <strong class="jopenegrita">open-access</strong> online platform which aims to unify Plant SynBio researchers’ work. Individual results can be published, supported by a <strong class="jopenegrita">modeling software</strong> tool integrated to ease the mathematical models’ generation of genetic circuits.</p>
 
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<h3>APPLIED DESIGN</h3>
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<h3>PLANTSYNBIO</h3>
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<p class="jopesangria">A genetic interface enables a bidirectional human-plant communication designing a modular optogenetic circuit and a color sensor system in plant chassis.</p>
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<p class="jopesangria">In our modeling, we focused on understanding and optimizing Chatterplant performance ensuring a smooth communication between plants and humans.</p>
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<h3>HARDWARE</h3>
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<p class="jopesangria">ChatterBox resulted from combining control over climatic conditions and SynBio with the objective of increasing sustainability.</p>
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<h3>SOFTWARE</h3>
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<p class="jopesangria">PlantLabCo is PlantSynBio's divulgation website, where we created a standard for information and tools for synthetic biology in plants.</p>
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<h3>HUMAN PRACTICES</h3>
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<p class="jopesangria">Several studies provided us with the information for development and integration of Chatterplant in the society.</p>
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Revision as of 20:23, 1 November 2017

ABSTRACT

Urban overpopulation, climate change and natural resources decrement are threatening food security. Ensuring season-less, accessible and local food production promotes a sustainable agriculture. Valencia_UPV provides a whole new system to control plant physiology at both genetic and environmental level.

ChatterPlant is a SynBio-based solution that works as plant-human interface allowing a bidirectional communication. First, a root-specific modular optogenetic circuit enables control on plants´ endogenous gene expression (e.g flowering). Then, a sensor circuit with color coded output provides specific information of stress conditions, accelerating corrective measures. The genetic setup is complemented with a hardware device, ChatterBox, specially designed to control plant’s growth conditions.

ChatterPlant’s possibilities can be improved gathering Plant SynBio knowledge. PlantLabCo is an open-access online platform which aims to unify Plant SynBio researchers’ work. Individual results can be published, supported by a modeling software tool integrated to ease the mathematical models’ generation of genetic circuits.