The purpose of our iGEM project is to modify the secondary sewage treatment process in a way that eliminates the inefficiencies created by the dynamic between aerobic nitrifying bacteria and heterotrophic denitrifying bacteria. Sewage Treatment systems employ processes designed around bacteria that occupy specific niches to treat human wastewater. The Biological Nutrient Removal stage in wastewater treatment uses bacteria capable of nitrification and denitrification to remove dissolved nitrogen from secondary effluent sewage. The process of denitrification has been found in both aerobic and anaerobic organisms, and major steps of the denitrification pathway have been elucidated in multiple strains (Potter; Brons; Costa; Gavira; Hasegawa; Moreno-Vivián) . Denitrification is the progressive anaerobic reduction of nitrates to nitrites to nitric oxide, nitrous oxide, and eventually nitrogen gas (Peng et al 2006). Nitrification involves the oxidation of ammonia into hydroxylamine and hydroxylamine into nitrites (Arp et al 2002). Our primary goal was to bring the capabilities of nitrification and denitrification into a single cell.