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− | < | + | <center><h1>Improve</h1></center> <br> |
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− | < | + | <p>Improved part from Virginia 2017: |
− | + | <a href="http://parts.igem.org/Part:BBa_K2272019">BBa_K2272019</a></p><br> | |
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− | The two parts from | + | The two parts from iGEM13 DTU-Denmark, <a href="http://parts.igem.org/Part:BBa_K1067002" width="100" height="100">BBa_K1067002</a> and <a href="http://parts.igem.org/Part:BBa_K1067004" width="100" height="100">BBa_K1067004</a>, are protein coding sequences extracted from the genome of Nitrosomonas europaea that code for nitrification enzymes: hydroxylamine oxidoreductase and cytochrome cycA&X. The homolog developed by DTU-Denmark used what we believed to be an improper coding sequence for a one of the subunits, HAO-B. HAO-B is a putative protein, that at the time may not have been believed to be significant. However, after reviewing the sequence registered in the BLAST database, we believed that HAO-B played a fundamental role in the mechanism of HAO. Since the two parts work hand in hand to nitrify ammonium, we improved the parts from DTU-Denmark by combining the two parts, inserting conditionally inducible promoters, and using signal peptides to transport the enzymes to their designated location in the cell.</p> |
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<img src="https://static.igem.org/mediawiki/parts/4/44/Virginia_pvhb_hao_cyt.png"> | <img src="https://static.igem.org/mediawiki/parts/4/44/Virginia_pvhb_hao_cyt.png"> | ||
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Latest revision as of 14:57, 1 November 2017
Improve
Improved part from Virginia 2017: BBa_K2272019
The two parts from iGEM13 DTU-Denmark, BBa_K1067002 and BBa_K1067004, are protein coding sequences extracted from the genome of Nitrosomonas europaea that code for nitrification enzymes: hydroxylamine oxidoreductase and cytochrome cycA&X. The homolog developed by DTU-Denmark used what we believed to be an improper coding sequence for a one of the subunits, HAO-B. HAO-B is a putative protein, that at the time may not have been believed to be significant. However, after reviewing the sequence registered in the BLAST database, we believed that HAO-B played a fundamental role in the mechanism of HAO. Since the two parts work hand in hand to nitrify ammonium, we improved the parts from DTU-Denmark by combining the two parts, inserting conditionally inducible promoters, and using signal peptides to transport the enzymes to their designated location in the cell.