Difference between revisions of "Team:Virginia/Improve"
| Line 8: | Line 8: | ||
<br></br> | <br></br> | ||
<ul> | <ul> | ||
| + | <p>Improved part from Virginia 2017: | ||
| + | <a href="http://parts.igem.org/Part:BBa_K2272019">BBa_K2272019</a></p><br> | ||
<p> | <p> | ||
The two parts from iGEM13 DTU-Denmark, <a href="http://parts.igem.org/Part:BBa_K1067002" width="100" height="100">BBa_K1067002</a> and <a href="http://parts.igem.org/Part:BBa_K1067004" width="100" height="100">BBa_K1067004</a>, are protein coding sequences extracted from the genome of Nitrosomonas europaea that code for nitrification enzymes: hydroxylamine oxidoreductase and cytochrome cycA&X. The homolog developed by DTU-Denmark used what we believed to be an improper coding sequence for a one of the subunits, HAO-B. HAO-B is a putative protein, that at the time may not have been believed to be significant. However, after reviewing the sequence registered in the BLAST database, we believed that HAO-B played a fundamental role in the mechanism of HAO. Since the two parts work hand in hand to nitrify ammonium, we improved the parts from DTU-Denmark by combining the two parts, inserting conditionally inducible promoters, and using signal peptides to transport the enzymes to their designated location in the cell.</p> | The two parts from iGEM13 DTU-Denmark, <a href="http://parts.igem.org/Part:BBa_K1067002" width="100" height="100">BBa_K1067002</a> and <a href="http://parts.igem.org/Part:BBa_K1067004" width="100" height="100">BBa_K1067004</a>, are protein coding sequences extracted from the genome of Nitrosomonas europaea that code for nitrification enzymes: hydroxylamine oxidoreductase and cytochrome cycA&X. The homolog developed by DTU-Denmark used what we believed to be an improper coding sequence for a one of the subunits, HAO-B. HAO-B is a putative protein, that at the time may not have been believed to be significant. However, after reviewing the sequence registered in the BLAST database, we believed that HAO-B played a fundamental role in the mechanism of HAO. Since the two parts work hand in hand to nitrify ammonium, we improved the parts from DTU-Denmark by combining the two parts, inserting conditionally inducible promoters, and using signal peptides to transport the enzymes to their designated location in the cell.</p> | ||
| Line 13: | Line 15: | ||
</div> | </div> | ||
<div class="column half_size"> | <div class="column half_size"> | ||
| − | <img src="https://static.igem.org/mediawiki/parts/ | + | <img src="https://static.igem.org/mediawiki/parts/4/44/Virginia_pvhb_hao_cyt.png"> |
| − | + | ||
</ul> | </ul> | ||
| Line 21: | Line 22: | ||
<br></br> | <br></br> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
</div> | </div> | ||
Latest revision as of 14:57, 1 November 2017
Improve
Improved part from Virginia 2017: BBa_K2272019
The two parts from iGEM13 DTU-Denmark, BBa_K1067002 and BBa_K1067004, are protein coding sequences extracted from the genome of Nitrosomonas europaea that code for nitrification enzymes: hydroxylamine oxidoreductase and cytochrome cycA&X. The homolog developed by DTU-Denmark used what we believed to be an improper coding sequence for a one of the subunits, HAO-B. HAO-B is a putative protein, that at the time may not have been believed to be significant. However, after reviewing the sequence registered in the BLAST database, we believed that HAO-B played a fundamental role in the mechanism of HAO. Since the two parts work hand in hand to nitrify ammonium, we improved the parts from DTU-Denmark by combining the two parts, inserting conditionally inducible promoters, and using signal peptides to transport the enzymes to their designated location in the cell.
