Difference between revisions of "Team:Virginia/Improve"

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<center><h1>Improve</h1></center> <br>
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<p>Improved part from Virginia 2017:
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<a href="http://parts.igem.org/Part:BBa_K2272019">BBa_K2272019</a></p><br>
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The two parts from iGEM13 DTU-Denmark, <a href="http://parts.igem.org/Part:BBa_K1067002" width="100" height="100">BBa_K1067002</a> and <a href="http://parts.igem.org/Part:BBa_K1067004" width="100" height="100">BBa_K1067004</a>, are protein coding sequences extracted from the genome of Nitrosomonas europaea that code for nitrification enzymes: hydroxylamine oxidoreductase and cytochrome cycA&X. The homolog developed by DTU-Denmark used what we believed to be an improper coding sequence for a one of the subunits, HAO-B.  HAO-B is a putative protein, that at the time may not have been believed to be significant.  However, after reviewing the sequence registered in the BLAST database, we believed that HAO-B played a fundamental role in the mechanism of HAO. Since the two parts work hand in hand to nitrify ammonium, we improved the parts from DTU-Denmark by combining the two parts, inserting conditionally inducible promoters, and using signal peptides to transport the enzymes to their designated location in the cell.</p>
  
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<h3>★  ALERT! </h3>
 
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
 
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
 
 
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<h1>Improve</h1>
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Regisrty. Please include a link to your improved part on this page.</p>
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<h3>Gold Medal Criterion #2</h3>
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<p><b>Standard Tracks:</b> Improve the function of an existing BioBrick Part. The original part must NOT be from your 2017 part number range. If you change the original part sequence, you must submit a new part. In addition, both the new and original part pages must reference each other. This working part must be different from the part documented in bronze #4 and silver #1.
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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Latest revision as of 14:57, 1 November 2017



Improve




    Improved part from Virginia 2017: BBa_K2272019


    The two parts from iGEM13 DTU-Denmark, BBa_K1067002 and BBa_K1067004, are protein coding sequences extracted from the genome of Nitrosomonas europaea that code for nitrification enzymes: hydroxylamine oxidoreductase and cytochrome cycA&X. The homolog developed by DTU-Denmark used what we believed to be an improper coding sequence for a one of the subunits, HAO-B. HAO-B is a putative protein, that at the time may not have been believed to be significant. However, after reviewing the sequence registered in the BLAST database, we believed that HAO-B played a fundamental role in the mechanism of HAO. Since the two parts work hand in hand to nitrify ammonium, we improved the parts from DTU-Denmark by combining the two parts, inserting conditionally inducible promoters, and using signal peptides to transport the enzymes to their designated location in the cell.