Difference between revisions of "Team:Virginia/Part Collection"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<center><h1>Part Collection</h1></center> <br>
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<p>This part collection includes reporter circuits of newly introduced promoters, functional nitrification pathway enzymes coding sequence, and the final combination of promoters and protein coding sequence. Future team can build more complex nitrification circuits with additional desired functions based on our part collection. This collection should serve as a base for further research related to nitrification and denitrification pathways, as well as be considered as a significant step taken towards an ideally constructed circuit.</p>
  
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<h1>Basic Parts</h1>
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<li><a href="http://parts.igem.org/Part:BBa_K2272000">BBa_K2272000</a> - a promoter that is maximally induced under microaerobic condition and regulates the expression of VHb, a bacterial hemoglobin that acts as a terminal oxidase. </li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272001">BBa_K2272001</a> - a promoter that regulates the production of MnSOD (mitochondrial antioxidant manganese superoxide dismutase), which detoxify radical oxygen produced by mitochondrial respiration.</li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272002">BBa_K2272002</a> - this part encodes Hydroxylamine Oxidoreductase (HAO), which catalyzes the oxidation of hydroxylamine to nitrite</li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272003">BBa_K2272003</a> - this part encodes two cytochromes that facilitate electron transport during the nitrification process</li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272004">BBa_K2272004</a> - this sequence from <i>Paracoccus denitrificans</i> codes for a signal peptide that will export the tagged protein to the periplasm of the cell</li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272005">BBa_K2272005</a> - this sequence from <i>Nitrosomonas europaea</i> codes for a signal peptide that exports the tagged protein to the periplasm of the cell</li>
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<h1>Composite Parts</h1>
  
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<li><a href="http://parts.igem.org/Part:BBa_K2272007">BBa_K2272007</a> - a reporter circuit for basic part BBa_K2272005 </li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272009">BBa_K2272009</a> - another reporter circuit for BBa_K2272005</li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272010">BBa_K2272010</a> - a reporter circuit for basic part BBa_K2272004</li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272008">BBa_K2272008</a> - another reporter circuit for BBa_K2272004</li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272011">BBa_K22720011</a> - a reporter circuit for BBa_K2272005</li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272006">BBa_K2272006</a> - a reporter circuit for BBa_K2272004</li>
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<h1> Part Collection </h1>
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<li><a href="http://parts.igem.org/Part:BBa_K2272012">BBa_K2272012</a> - a reporter circuit for BBa_K2272001</li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272013">BBa_K2272013</a> - a reporter circuit for BBa_K2272000</li>
  
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<li><a href="http://parts.igem.org/Part:BBa_K2272014">BBa_K2272014</a> - Cytochrome cycA and cycX coding sequence regulated by arabinose-inducible promoter from the distribution kit, pBAD. </li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272015">BBa_K2272015</a> - Cytochrome cycA and cycX coding sequence regulated by the microaerobic conditioned promoter VHb. </li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272016">BBa_K2272016</a> - Cytochrome cycA and cycX coding sequence regulated by hypoxic conditioned promoter, MnSOD. </li>
  
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
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<li><a href="http://parts.igem.org/Part:BBa_K2272017">BBa_K2272017</a> - Coding sequence of hydroxylamine oxidoreductase regulated by hypoxic conditioned promoter, MnSOD. </li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272018">BBa_K2272018</a> - Coding sequence of hydroxylamine oxidoreductase regulated by arabinose-inducible promoter, pBAD. </li>
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<li><a href="http://parts.igem.org/Part:BBa_K2272019">BBa_K2272019</a> - an composite version of BBa_K1067002 and BBa_K1067004</li>
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While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.
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<h3>Best Part Collection Special Prize</h3>
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<p>Did your team make a lot of great parts? Is there a team that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection or a collection of awesome pigment parts? Tell the judges you should be evaluated for the Best Parts Collection award! To be eligible for this award, these parts must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts.
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If you have a collection of parts you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part numbers to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.</p>
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<center><groupparts>iGEM17 Virginia</groupparts></center>
  
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<h4>Note</h4>
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<p>This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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Revision as of 02:43, 1 November 2017



Part Collection



This part collection includes reporter circuits of newly introduced promoters, functional nitrification pathway enzymes coding sequence, and the final combination of promoters and protein coding sequence. Future team can build more complex nitrification circuits with additional desired functions based on our part collection. This collection should serve as a base for further research related to nitrification and denitrification pathways, as well as be considered as a significant step taken towards an ideally constructed circuit.


Basic Parts

  • BBa_K2272000 - a promoter that is maximally induced under microaerobic condition and regulates the expression of VHb, a bacterial hemoglobin that acts as a terminal oxidase.
  • BBa_K2272001 - a promoter that regulates the production of MnSOD (mitochondrial antioxidant manganese superoxide dismutase), which detoxify radical oxygen produced by mitochondrial respiration.
  • BBa_K2272002 - this part encodes Hydroxylamine Oxidoreductase (HAO), which catalyzes the oxidation of hydroxylamine to nitrite
  • BBa_K2272003 - this part encodes two cytochromes that facilitate electron transport during the nitrification process
  • BBa_K2272004 - this sequence from Paracoccus denitrificans codes for a signal peptide that will export the tagged protein to the periplasm of the cell
  • BBa_K2272005 - this sequence from Nitrosomonas europaea codes for a signal peptide that exports the tagged protein to the periplasm of the cell

Composite Parts


  • BBa_K2272012 - a reporter circuit for BBa_K2272001
  • BBa_K2272013 - a reporter circuit for BBa_K2272000
  • BBa_K2272014 - Cytochrome cycA and cycX coding sequence regulated by arabinose-inducible promoter from the distribution kit, pBAD.
  • BBa_K2272015 - Cytochrome cycA and cycX coding sequence regulated by the microaerobic conditioned promoter VHb.
  • BBa_K2272016 - Cytochrome cycA and cycX coding sequence regulated by hypoxic conditioned promoter, MnSOD.
  • BBa_K2272017 - Coding sequence of hydroxylamine oxidoreductase regulated by hypoxic conditioned promoter, MnSOD.
  • BBa_K2272018 - Coding sequence of hydroxylamine oxidoreductase regulated by arabinose-inducible promoter, pBAD.
  • BBa_K2272019 - an composite version of BBa_K1067002 and BBa_K1067004

<groupparts>iGEM17 Virginia</groupparts>