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Revision as of 18:00, 7 October 2017
Safety
Please visit the main Safety page to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.
On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can go beyond the questions on the safety forms, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)
Safe Project Design
Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:
- Choosing a non-pathogenic chassis
- Choosing parts that will not harm humans / animals / plants
- Substituting safer materials for dangerous materials in a proof-of-concept experiment
- Including an "induced lethality" or "kill-switch" device
Safe Lab Work
What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!
Safe Shipment
Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?
Risk Groups
We chose Paraccocus denitrificans as our chassis device. It is a risk group 1 organism and is non-pathogenic. We ensured that all parts we are using come from Nitrosomonas europaea and Escherichia coli (K12 strain), both risk group 1 organisms.
Containment
Our device is designed to be implemented in a wastewater facility. Currently, multi-cultures are used in wastewater facilities, so containment for the bacteria has already been designed into the facilities. In most facilities, there are two methods to remove the bacteria from the cleaned water. The first method uses chlorine. One part of the facility will insert chlorine into the processed water, oxidizing cellular material of the bacteria. This procedure is often used because it is cost-effective and well-established. Another method of removal uses UV light. UV light prevents the bacteria from reproducing by damaging the DNA and RNA of the bacteria. This procedure is often used because it leaves no harmful residue in the water and is highly effective.
Our Lab
We are working in a Biosafety level 2 lab.
