Revision as of 03:35, 16 August 2017

Description

Part: BBa_

Brief introduction in RdhANP

RdhANP is a native protein of Nitratireducror pacificus pht-3B. It is codon-optimized for operated and expression in Bacillus magaterium. RdhANP can catalyze the reduction of ortho-halogenated phenolic compound, which endows it with biological dehalogenation function. Different from other reductive proteins that are membrane-associated and oxygen-sensitive in their family, RdhANP is a soluble cytoplasmic one and oxygen-tolerant, which is fairly good for heterologous expression in other chassis. However, this enzyme displays a strict requirement for o-halogenated phenolic substrates and B12 cofactor; it also should under reductive condition using either reduced methyl viologen or NADPH.

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 <h6>Brief introduction in RdhANP</h6>
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-<li>Halogenated phenols are a group of organic molecules, characterized by benzene rings with hydroxyl groups and halogen atoms. In general, halogenated phenols tend to be more toxic and less biodegradable than their nonhalogenated counterparts, to note that phenols, cresols, and catechols do not bioaccumulate to any great extent. Used in large quantities for diverse purposes and moderately soluble in water, fugitive phenolics are inevitable and these can be moved around readily by water. It’s been reported that halogenated phenols have accumulated in various anoxic subsurface environments as a consequence of improper disposal. What’s even worse, increasing levels of halogenated phenol detected in humans and wildlife are of particular concern. With an original dehalogenase named RdhANP from Nitratireductor pacificus, we aim to engineer selected microbes to build a dehalogenater, to remove halogen atoms from halogenated phenols, thus reducing their toxicity and facilitate their subsequent treatment. Compared with other enzymes in the dehalogenase subfamily, which are usually membrane-associated and oxygen-sensitive, RdhANP is a soluble, oxygen-tolerant reductive dehalogenase depending on cobalamin (B12) and electron donors (i.e NADPH) for its catalytic activity. After successful heterologous expression of RdhANP, we’d design our dehalogenator to function efficiently in suitable devices.</li>
+<li>RdhANP is a native protein of Nitratireducror pacificus pht-3B. It is codon-optimized for operated and expression in Bacillus magaterium. RdhANP can catalyze the reduction of ortho-halogenated phenolic compound, which endows it with biological dehalogenation function. Different from other reductive proteins that are membrane-associated and oxygen-sensitive in their family, RdhANP is a soluble cytoplasmic one and oxygen-tolerant, which is fairly good for heterologous expression in other chassis. However, this enzyme displays a strict requirement for o-halogenated phenolic substrates and B12 cofactor; it also should under reductive condition using either reduced methyl viologen or NADPH.</li>
 </ul>