Difference between revisions of "Team:WHU-China/Description"

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<h5>Description of our operating bacteria</h5>
 
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<li>Nitratireductor pacificus strain pht-3BT (MCCC 1A01024T, CCTCC AB 209302T, LMG 25541T) was isolated from deep sea sediments of the western Pacific Ocean enriched with pyrene as the sole carbon and energy source. Cells were Gram-negative, short rods that were motile by means of flagella. They were first grown on marine agar 2216 (BD) medium, which produces smooth grey non-pigmented colonies with regular edges and slightly raised in the center, 2–3 mm in diameter after 3 days at 28 ℃ .We use MB medium as alternative. The most important feature of the cell is its production of RdhANP. </li>
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<h5>References</h5>
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<h5>Modularization: Overview</h5>
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Revision as of 03:38, 16 August 2017

Description

Part: BBa_

Brief introduction in RdhANP
  • RdhANP is a native protein of Nitratireducror pacificus pht-3B. It is codon-optimized for operated and expression in Bacillus magaterium. RdhANP can catalyze the reduction of ortho-halogenated phenolic compound, which endows it with biological dehalogenation function. Different from other reductive proteins that are membrane-associated and oxygen-sensitive in their family, RdhANP is a soluble cytoplasmic one and oxygen-tolerant, which is fairly good for heterologous expression in other chassis. However, this enzyme displays a strict requirement for o-halogenated phenolic substrates and B12 cofactor; it also should under reductive condition using either reduced methyl viologen or NADPH.
Description of our operating bacteria
  • Nitratireductor pacificus strain pht-3BT (MCCC 1A01024T, CCTCC AB 209302T, LMG 25541T) was isolated from deep sea sediments of the western Pacific Ocean enriched with pyrene as the sole carbon and energy source. Cells were Gram-negative, short rods that were motile by means of flagella. They were first grown on marine agar 2216 (BD) medium, which produces smooth grey non-pigmented colonies with regular edges and slightly raised in the center, 2–3 mm in diameter after 3 days at 28 ℃ .We use MB medium as alternative. The most important feature of the cell is its production of RdhANP.
Modularization: Overview
Inspiration

See how other teams have described and presented their projects: