Difference between revisions of "Team:WHU-China/Description"

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              <a>Project</a>
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                <li id="nv11"><a href="#">Description</a></li>
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                <li id="nv12"><a href="#">Design</a></li>
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              <a>Human Practices</a>
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<h6>Description</h6>
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  <li><a href="#Brief">1 Brief introduction in RdhANP</a></li>
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  <li><a href="#Description">2 Description of our operating bacteria</a></li>
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  <li><a href="#Modularization">3 Modularization: Overview</a></li>
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  <li><a href="#protocol">4 Our protocol</a></li>
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  <li><a href="#Appendix">5 Appendix</a></li>
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          <h1 id="Brief">Brief introduction in RdhANP</h1>
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          <p>RdhANP is a native protein of Nitratireducror pacificus pht-3B. It is codon-optimized for operated and expression in Bacillus magaterium. RdhANP can catalyze the reduction of ortho-halogenated phenolic compound, which endows it with biological dehalogenation function. Different from other reductive proteins that are membrane-associated and oxygen-sensitive in their family, RdhANP is a soluble cytoplasmic one and oxygen-tolerant, which is fairly good for heterologous expression in other chassis. However, this enzyme displays a strict requirement for o-halogenated phenolic substrates and B12 cofactor; it also should under reductive condition using either reduced methyl viologen or NADPH. </p>
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<td><strong>#1 Integrated Human Practices</strong><br>Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project.</td>
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<td>Faced with the people's overwhelming concerns regarding the application of genetic engineering, we strived to improve upon our project design of Noro-catcher and introduced a new module to increase its safety. We also have conducted a lecture/workshop to students of age 13-18, and observed that their overall acceptance of genetic engineering increased by introducing them to basic knowledge on the matter. Visit <a href="https://2016.igem.org/Team:Kyoto/Integrated_Practices" target="_blank">Integrated Practices</a> and <a href="https://2016.igem.org/Team:Kyoto/HP/Gold" target="_blank">HP/Gold</a> for details.  </td>
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<td><strong>#2 Improve a previous part or project</strong><br>Improve the function OR characterization of an existing BioBrick Part or Device and enter this information in the Registry. Please see the Registry help page on how to document a contribution to an existing part. This part must NOT be from your 2016 part number range.</td>
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<td>We enhanced iGEM12_Penn's INPNC-MCS to INPNC-6xHistag and improved its function. Visit <a href="http://parts.igem.org/Part:BBa_K811005" target="_blank">the original parts page</a> and <a href="http://parts.igem.org/Part:BBa_K1933001" target="_blank">our enhanced parts page</a> for details</td>
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<td><strong>#3 Proof of concept</strong><br>Demonstrate a functional proof of concept of your project. Your proof of concept must consist of a BioBrick device; a single BioBrick part cannot constitute a proof of concept. (biological materials may not be taken outside the lab).</td>
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<td>Our ultimate goal was to create <i>E. coli</i> which can bind to NoV, and improved upon surface expression systems INPNC and BclA to achieve this. In detail, we enabled the purification and detection of the fusion protein by the addition of His tag, regardless of the passenger proteins placed downstream. Furthermore, we observed the binding of scFv surface expressing <i>E. coli</i> to NoVLP using SEM. Visit our <a href="https://2016.igem.org/Team:Kyoto/Proof" target="_blank">Proof of concept </a>and <a href="http://parts.igem.org/Part:BBa_K1933200" target="_blank">Parts</a> for details.  </td>
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Revision as of 05:50, 24 August 2017

Description

Brief introduction in RdhANP

RdhANP is a native protein of Nitratireducror pacificus pht-3B. It is codon-optimized for operated and expression in Bacillus magaterium. RdhANP can catalyze the reduction of ortho-halogenated phenolic compound, which endows it with biological dehalogenation function. Different from other reductive proteins that are membrane-associated and oxygen-sensitive in their family, RdhANP is a soluble cytoplasmic one and oxygen-tolerant, which is fairly good for heterologous expression in other chassis. However, this enzyme displays a strict requirement for o-halogenated phenolic substrates and B12 cofactor; it also should under reductive condition using either reduced methyl viologen or NADPH.