Difference between revisions of "Team:WHU-China/Description"
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Revision as of 05:51, 24 August 2017
Description
- 1 Brief introduction in RdhANP
- 2 Description of our operating bacteria
- 3 Modularization: Overview
- 4 Our protocol
- 5 Appendix
Brief introduction in RdhANP
RdhANP is a native protein of Nitratireducror pacificus pht-3B. It is codon-optimized for operated and expression in Bacillus magaterium. RdhANP can catalyze the reduction of ortho-halogenated phenolic compound, which endows it with biological dehalogenation function. Different from other reductive proteins that are membrane-associated and oxygen-sensitive in their family, RdhANP is a soluble cytoplasmic one and oxygen-tolerant, which is fairly good for heterologous expression in other chassis. However, this enzyme displays a strict requirement for o-halogenated phenolic substrates and B12 cofactor; it also should under reductive condition using either reduced methyl viologen or NADPH.
