Difference between revisions of "Team:Washington/Wetlab"

Latest revision as of 02:36, 2 November 2017

Washington iGEM

Wetlab

Introduction

The Washington iGEM wetlab team is in charge of the molecular biology side of the project. We developed a dCas9-sgRNA system designed to be inducible in yeast. Taking advantage of the violacein pathway as a proxy, we produced different colors to track metabolic activity. We also created and characterized Biobricks as well as Interlab.

About

Click on a circle to learn more!

gRNA Expression

We used the CRISPR/dCas9 system to regulate the violacein pathway in yeast using inducible promoters.

Design

Our team encountered multiple design constraints in the violacein pathway and ultimately found that the CRISPR/dCas9 system paired with sgRNAs controlled by the promoters ZAP1 and CUP1 was the most effective method.

InterLab

This year, our team successfully completed InterLab.

BioBricks

We created yeast promoter BioBricks!

Conclusion

This season, Washington iGEM’s new wetlab team had a productive summer and fall. Having received an influx of new members we happily trained and worked hard alongside one another. For our main project, we were able to create the sgRNA expression system and successfully integrated it in yeast. We created and characterized two novel inducible promoter biobricks for yeast. Additionally, for the first time ever, we were able to participate in the iGEM 2017 Interlab collaboration alongside other iGEM teams across the globe.

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 		  		<h4 class="subSection">Introduction</h4>
-		  		Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod
+		  		<p>The Washington iGEM wetlab team is in charge of the molecular biology side of the project. We developed a dCas9-sgRNA system designed to be inducible in yeast. Taking advantage of the violacein pathway as a proxy, we produced different colors to track metabolic activity. We also created and characterized Biobricks as well as Interlab.</p>
-		  		tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam,
-		  		quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo
-		  		consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse
-		  		cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non
-		  		proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
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 				  	<div class="row justify-content-md-center">
 				  		<div class="col-md-auto" style="text-align: center">
-				  			<div class="col" style="margin: 3px"><div class="engagementContent rounded-circle" style="margin: auto; background-color:white"><a href="/Team:Washington/gRNA_Expression"><span><div class="rounded-circle pictureEngagement" style="background:url('https://static.igem.org/mediawiki/2017/f/ff/T--Washington--PCBDesign.jpg') no-repeat; background-position: 48% 50%; background-size: 400px auto" ></div></span></a></div></div>
+				  			<div class="col" style="margin: 3px"><div class="engagementContent rounded-circle" style="margin: auto; background-color:white"><a href="/Team:Washington/gRNA_Expression"><span><div class="rounded-circle pictureEngagement" style="background:url('https://static.igem.org/mediawiki/2017/5/53/T--Washington--ViolaceinPathway.png') no-repeat; background-position: 65% 50%; background-size: 250px auto" ></div></span></a></div></div>
-							<p style="text-align: center"><b>gRNA Expression</b><div class="container" style="max-width: 250px">Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod
+							<p style="text-align: center"><b>gRNA Expression</b><div class="container" style="max-width: 250px">We used the CRISPR/dCas9 system to regulate the violacein pathway in yeast using inducible promoters.</div><br/></p><br />
-							tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam,
-							quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo
-							consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse
-							cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non
-							proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</div><br/></p><br />
 						</div>
 						<div class="col-md-auto" style="text-align: center">
-				  			<div class="col" style="margin: 3px"><div class="engagementContent rounded-circle" style="margin: auto; background-color:white"><a href="/Team:Washington/Design#Wetlab"><span><div class="rounded-circle pictureEngagement" style="background:url('https://static.igem.org/mediawiki/2017/3/31/T--Washington--DesignDrawing2.png') no-repeat; background-position: 48% 50%; background-size: 250px auto" ></div></span></a></div></div>
+				  			<div class="col" style="margin: 3px"><div class="engagementContent rounded-circle" style="margin: auto; background-color:white"><a href="/Team:Washington/Design#Wetlab"><span><div class="rounded-circle pictureEngagement" style="background:url('https://static.igem.org/mediawiki/2017/8/8f/T--Washington--SGRNADiagram.jpg') no-repeat; background-position: 48% 50%; background-size: 250px auto" ></div></span></a></div></div>
-							<p style="text-align: center"><b>Design</b><div class="container" style="max-width: 250px">This year, the UW iGEM team has created the Chromastat: an affordable, modular, and autonomous turbidostat + color detector.</div><br/></p><br />
+							<p style="text-align: center"><b>Design</b><div class="container" style="max-width: 250px">Our team encountered multiple design constraints in the violacein pathway and ultimately found that the CRISPR/dCas9 system paired with sgRNAs controlled by the promoters ZAP1 and CUP1 was the most effective method.</div><br/></p><br />
 						</div>
 						<div class="col-md-auto" style="text-align: center">
-				  			<div class="col" style="margin: 3px"><div class="engagementContent rounded-circle" style="margin: auto; background-color:white"><a href="/Team:Washington/InterLab"><span><div class="rounded-circle pictureEngagement" style="background:url('https://static.igem.org/mediawiki/2017/c/c5/T--Washington--DesignDrawing1.png') no-repeat; background-position: 48% 50%; background-size: 250px auto" ></div></span></a></div></div>
+				  			<div class="col" style="margin: 3px"><div class="engagementContent rounded-circle" style="margin: auto; background-color:white"><a href="/Team:Washington/InterLab"><span><div class="rounded-circle pictureEngagement" style="background:url('https://static.igem.org/mediawiki/2017/d/d9/T--Washington--GraphForTile.png') no-repeat; background-position: 48% 50%; background-size: 250px auto" ></div></span></a></div></div>
-							<p style="text-align: center"><b>InterLab</b><div class="container" style="max-width: 250px">Our software is both easy-to-use and easy to modify. User-friendliness was a high priority - much more so than similar ad hoc scientific tools. By using Oracle standard Java software development idioms and thorough JavaDoc code documentation, we’ve created a codebase that is easily adjustable by anyone with a modicum of Java experience.</div><br/></p><br />
+							<p style="text-align: center"><b>InterLab</b><div class="container" style="max-width: 250px">This year, our team successfully completed InterLab.</div><br/></p><br />
 						</div>
 						<div class="col-md-auto" style="text-align: center">
-				  			<div class="col" style="margin: 3px"><div class="engagementContent rounded-circle" style="margin: auto; background-color:white"><a href="/Team:Washington/BioBricks"><span><div class="rounded-circle pictureEngagement" style="background:url('https://static.igem.org/mediawiki/2017/c/c5/T--Washington--DesignDrawing1.png') no-repeat; background-position: 48% 50%; background-size: 250px auto" ></div></span></a></div></div>
+				  			<div class="col" style="margin: 3px"><div class="engagementContent rounded-circle" style="margin: auto; background-color:white"><a href="/Team:Washington/BioBricks"><span><div class="rounded-circle pictureEngagement" style="background:url('https://static.igem.org/mediawiki/2017/f/f0/T--Washington--BioBricksTile.png') no-repeat; background-position: 0% 50%; background-size: 250px auto" ></div></span></a></div></div>
-							<p style="text-align: center"><b>BioBricks</b><div class="container" style="max-width: 250px">Our software is both easy-to-use and easy to modify. User-friendliness was a high priority - much more so than similar ad hoc scientific tools. By using Oracle standard Java software development idioms and thorough JavaDoc code documentation, we’ve created a codebase that is easily adjustable by anyone with a modicum of Java experience.</div><br/></p><br />
+							<p style="text-align: center"><b>BioBricks</b><div class="container" style="max-width: 250px">We created yeast promoter BioBricks!</div><br/></p><br />
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 					</div>
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   				  <div id="Conclusion" class="jumpSection"></div>
 			  	  <h4 class="subSection">Conclusion</h4>
-  				  <p> "Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum." </p>
+  				  <p>This season, Washington iGEM’s new wetlab team had a productive summer and fall. Having received an influx of new members we happily trained and worked hard alongside one another. For our main project, we were able to create the sgRNA expression system and successfully integrated it in yeast. We created and characterized two novel inducible promoter biobricks for yeast. Additionally, for the first time ever, we were able to participate in the iGEM 2017 Interlab collaboration alongside other iGEM teams across the globe. </p>
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 		</div>