Difference between revisions of "Team:Worldshaper-Wuhan/InterLab"

 
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{{Worldshaper-Wuhan}}
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<section class="show-products-category-navigation page-borders-thick canvas-style-normal header-subtitle-none banner-alignment-center blog-layout-center project-layout-left-sidebar thumbnails-on-open-page-show-all social-icon-style-round  hide-info-footer  hide-article-author  event-show-past-events event-thumbnails event-thumbnail-size-32-standard event-date-label  event-list-show-cats event-list-date event-list-time event-list-address  event-icalgcal-links  event-excerpts event-item-back-link product-list-titles-under product-list-alignment-center product-item-size-11-square  product-gallery-size-11-square product-gallery-auto-crop show-product-price show-product-item-nav product-social-sharing newsletter-style-dark hide-opentable-icons opentable-style-dark small-button-style-solid small-button-shape-square medium-button-style-solid medium-button-shape-square large-button-style-solid large-button-shape-square image-block-poster-text-alignment-center image-block-card-dynamic-font-sizing image-block-card-content-position-center image-block-card-text-alignment-left image-block-overlap-dynamic-font-sizing image-block-overlap-content-position-center image-block-overlap-text-alignment-left image-block-collage-dynamic-font-sizing image-block-collage-content-position-top image-block-collage-text-alignment-left image-block-stack-dynamic-font-sizing image-block-stack-text-alignment-left button-style-outline button-corner-style-square tweak-product-quick-view-button-style-floating tweak-product-quick-view-button-position-bottom tweak-product-quick-view-lightbox-excerpt-display-truncate tweak-product-quick-view-lightbox-show-arrows tweak-product-quick-view-lightbox-show-close-button tweak-product-quick-view-lightbox-controls-weight-light native-currency-code-usd collection-type-page collection-5990133859cc681bb449c9ba collection-layout-default homepage mobile-style-available logo-image">
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<h1>InterLab</h1>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range.
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For teams participating in the <a href="https://2017.igem.org/Competition/InterLab_Study">InterLab study</a>, all work must be shown on this page.
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<h1 class="text-align-center">InterLab</h1>
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<div style="padding-bottom: 29.1971%; overflow: hidden;" class="image-block-wrapper  has-aspect-ratio" data-description="<p>OD600 reference point</p>
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<img src="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f69e3b0852291b22a4bf19/1509334596669/1.jpeg"  alt="OD600 reference point"  />
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<img class="thumb-image loaded" data-src="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f69e3b0852291b22a4bf19/1509334596669/1.jpeg" data-image="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f69e3b0852291b22a4bf19/1509334596669/1.jpeg" data-image-dimensions="1370x400" data-image-focal-point="0.5,0.5" data-load="false" data-image-id="59f69e3b0852291b22a4bf19" data-type="image" data-position-mode="standard" style="left: 0%; top: -0.0198042%; width: 100%; height: 100.04%; position: absolute;" alt="OD600 reference point" src="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f69e3b0852291b22a4bf19/1509334596669/1.jpeg?format=1000w" data-image-resolution="1000w"></div>
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<p>OD600 reference point</p>
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<div style="padding-bottom: 33.68%; overflow: hidden;" class="image-block-wrapper  has-aspect-ratio" data-description="<p>Fluorescein standard curve</p>
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<img src="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f69e4c8165f5c07f52252c/1509334739040/2.2.jpeg"  alt="Fluorescein standard curve"  />
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<img class="thumb-image loaded" data-src="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f69e4c8165f5c07f52252c/1509334739040/2.2.jpeg" data-image="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f69e4c8165f5c07f52252c/1509334739040/2.2.jpeg" data-image-dimensions="2500x842" data-image-focal-point="0.5,0.5" data-load="false" data-image-id="59f69e4c8165f5c07f52252c" data-type="image" data-position-mode="standard" style="left: -0.0451414%; top: 0%; width: 100.09%; height: 100%; position: absolute;" alt="Fluorescein standard curve" src="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f69e4c8165f5c07f52252c/1509334739040/2.2.jpeg?format=1000w" data-image-resolution="1000w"></div>
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<p>Fluorescein standard curve</p>
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<p>Worldshaper-Wuhan _InterLab_201</p>
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<div style="padding-bottom: 31.32%; overflow: hidden;" class="image-block-wrapper  has-aspect-ratio" data-description="<p>Fluorescence Measurement</p>
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" id="yui_3_17_2_1_1509335407202_204">
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<noscript>
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<img src="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f69e9cf9619ab1fcc63922/1509334688464/"  alt="Fluorescence Measurement"  />
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<img class="thumb-image loaded" data-src="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f69e9cf9619ab1fcc63922/1509334688464/" data-image="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f69e9cf9619ab1fcc63922/1509334688464/" data-image-dimensions="2500x783" data-image-focal-point="0.5,0.5" data-load="false" data-image-id="59f69e9cf9619ab1fcc63922" data-type="image" data-position-mode="standard" style="left: -0.0236935%; top: 0%; width: 100.047%; height: 100%; position: absolute;" alt="Fluorescence Measurement" src="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f69e9cf9619ab1fcc63922/1509334688464/?format=1000w" data-image-resolution="1000w"></div>
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<div class="image-caption-wrapper">
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<div class="image-caption">
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<p>Fluorescence Measurement</p>
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</div>
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</div>
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</div>
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<div class="sqs-block-content">
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<p>1.&nbsp;&nbsp;&nbsp; O0 Reference point</p>
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<p>
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Prepare a column of 4 wells with 100 µl 100% LUDOX and 4 wells containing 100 µl  . Repeat the measurement in all relevant modes used in your experiments.
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</p>
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<p>
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2.&nbsp;&nbsp;&nbsp; Protocol fluorescein fluorescence standard curve
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</p>
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<p>
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The same cuvettes, plates and volumes were used to avoid calibration errors. Next, we determined the fluorescein standard curve through dilution series in 4 different replicate rows. Using the settings for GFP, the 4 rows were measured and the results were used to determine the resulting curve.
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</p>
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<p>3.&nbsp;&nbsp;&nbsp; Test</p>
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<p>
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The protein GFP was analyzed using the InterLab parts found on Kit Plate 6. This test devices were transformed into the E. coli strain DH5 alpha. Using our defined parameters, we tested the expression of a positive control (BBa_I20270), negative control (BBa_R0040) and 6 different test devices.
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</p>
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<p>
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- BBa_J364000 (Test Device #1)
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<br>
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- BBa_J364001 (Test Device #2)
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<br>
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- BBa_J364002 (Test Device #3)
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<br>
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- BBa_J364003 (Test Device #4)
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<br>
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- BBa_J364004 (Test Device #5)
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<br>- BBa_J364005 (Test Device #6)</p>
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</div>
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Latest revision as of 08:30, 1 November 2017