1.    O0 Reference point

Prepare a column of 4 wells with 100 µl 100% LUDOX and 4 wells containing 100 µl . Repeat the measurement in all relevant modes used in your experiments.

2.    Protocol fluorescein fluorescence standard curve

The same cuvettes, plates and volumes were used to avoid calibration errors. Next, we determined the fluorescein standard curve through dilution series in 4 different replicate rows. Using the settings for GFP, the 4 rows were measured and the results were used to determine the resulting curve.

3.    Test

The protein GFP was analyzed using the InterLab parts found on Kit Plate 6. This test devices were transformed into the E. coli strain DH5 alpha. Using our defined parameters, we tested the expression of a positive control (BBa_I20270), negative control (BBa_R0040) and 6 different test devices.

- BBa_J364000 (Test Device #1)
- BBa_J364001 (Test Device #2)
- BBa_J364002 (Test Device #3)
- BBa_J364003 (Test Device #4)
- BBa_J364004 (Test Device #5)
- BBa_J364005 (Test Device #6)