Difference between revisions of "Team:Worldshaper-Wuhan/Model"
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<ul> | <ul> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/ | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/solution-and-design">Solution and Design</a> |
</li> | </li> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/ | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/Results">Results</a> |
</li> | </li> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/ | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/Model">Model</a> |
</li> | </li> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/ | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/Parts">Parts</a> |
</li> | </li> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/ | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/Demonstrate">Measurement</a> |
</li> | </li> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/ | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/procedure">Procedure</a> |
</li> | </li> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/ | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/Notebook">Notebook</a> |
</li> | </li> | ||
</ul> | </ul> | ||
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| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/Engagement"> | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/Engagement">Survey Result</a> |
</li> | </li> | ||
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<ul> | <ul> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/ | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/solution-and-design">Solution and Design</a> |
</li> | </li> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/ | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/Results">Results</a> |
</li> | </li> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/ | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/Model">Model</a> |
</li> | </li> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/ | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/Parts">Parts</a> |
</li> | </li> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/ | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/Demonstrate">Measurement</a> |
</li> | </li> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/ | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/procedure">Procedure</a> |
</li> | </li> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/ | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/Notebook">Notebook</a> |
</li> | </li> | ||
</ul> | </ul> | ||
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<li class="page-collection"> | <li class="page-collection"> | ||
| − | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/Engagement"> | + | <a href="https://2017.igem.org/Team:Worldshaper-Wuhan/Engagement">Survey Result</a> |
</li> | </li> | ||
<li class="page-collection"> | <li class="page-collection"> | ||
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<div class="sqs-block html-block sqs-block-html"> | <div class="sqs-block html-block sqs-block-html"> | ||
<div class="sqs-block-content"> | <div class="sqs-block-content"> | ||
| − | <h1 class="text-align-center"> | + | <h1 class="text-align-center">Model</h1> |
</div> | </div> | ||
| − | <div class="sqs-block-content"> | + | <div class="sqs-block-content"> |
| + | |||
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| + | <div style="padding-bottom: 102.279%; overflow: hidden;" class="image-block-wrapper has-aspect-ratio" data-description="<p> <em>Fig. Model for the effect of miR-21 sponge in cells. After introduction of the sponge transgene, sponge RNAs are expressed at a high level and attract miR-21, inhibiting the expression of eGFP reporter. The expression of miR-21 should be identified by their eGFP reporter expression.</em> | ||
| + | </p> | ||
| + | " id="yui_3_17_2_1_1509520478643_116"> | ||
| + | <noscript> | ||
| + | <img src="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f7dd8eec212d5a70e1bcf0/1509416775371/a.jpg" alt="Fig. Model for the effect of miR-21 sponge in cells. After introduction of the sponge transgene, sponge RNAs are expressed at a high level and attract miR-21, inhibiting the expression of eGFP reporter. The expression of miR-21 should be identified by their eGFP reporter expression." /> | ||
| + | </noscript> | ||
| + | <img class="thumb-image loaded" data-src="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f7dd8eec212d5a70e1bcf0/1509416775371/a.jpg" data-image="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f7dd8eec212d5a70e1bcf0/1509416775371/a.jpg" data-image-dimensions="1141x1167" data-image-focal-point="0.5,0.5" data-load="false" data-image-id="59f7dd8eec212d5a70e1bcf0" data-type="image" data-position-mode="standard" style="left: 0%; top: -0.00794987%; width: 100%; height: 100.016%; position: absolute;" alt="Fig. Model for the effect of miR-21 sponge in cells. After introduction of the sponge transgene, sponge RNAs are expressed at a high level and attract miR-21, inhibiting the expression of eGFP reporter. The expression of miR-21 should be identified by their eGFP reporter expression." src="https://static1.squarespace.com/static/598d6f47ff7c50fa2d55f1dc/t/59f7dd8eec212d5a70e1bcf0/1509416775371/a.jpg?format=1000w" data-image-resolution="1000w"></div> | ||
| + | |||
| + | <div class="image-caption-wrapper"> | ||
| + | <div class="image-caption"> | ||
| + | <p> <em>Fig. Model for the effect of miR-21 sponge in cells. After introduction of the sponge transgene, sponge RNAs are expressed at a high level and attract miR-21, inhibiting the expression of eGFP reporter. The expression of miR-21 should be identified by their eGFP reporter expression.</em> | ||
| + | </p> | ||
| + | </div> | ||
| + | </div> | ||
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| + | </div> | ||
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| + | <div class="sqs-block html-block sqs-block-html" data-block-type="2" id="block-8f5488099244885f0a60"> | ||
| + | <div class="sqs-block-content"> | ||
| + | <p> <strong>Model</strong> | ||
| + | </p> | ||
| + | <p> | ||
| + | We created a model which can measure the expression of miRNA in cells by using miRNA sponges. This model is very novel because it is the first time of using miRNA sponges to monitor the expression levels of miRNA. The principal of the model based on characteristic of sponge RNAs, which can attact miRNA and inhibit the expression of miRNA-target (Figure). | ||
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| − | + | <p>Wuhan, China . New York, USA</p> | |
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Latest revision as of 07:18, 1 November 2017
