Team:XJTLU-CHINA/Demonstrate
Demonstration
Aimed at examining the efficacy of the antimicrobial peptides on killing S. aureus (each experiment below were repeated 3 times).
For the whole circuit construct, we did following experiments:
1. Gel electrophoresis
1. Expression of LL-37 in bacteria by dot blotting
3. Inhibition Ring
This experiment qualitatively evaluate the effective of Anti-microbial peptides (AMPs). The result shows that the AMPs has effective on Staphylococcus aureus.
Figure 1 shows the AMPs has effective on Staphylococcus aureus, It seems that all of three AMPs has effective on Staphylococcus aureus.
2. Determination of the minimal inhibitory concentration (MIC) of the three AMPs.
Both single peptide of the three AMPs and the mixture of them have conspicuous lethal effect on S. aureus.
3. Absorbance (cell density) of the biofilm of S. aureus against different concentrations of the three individual AMPs or a mixture of them
The biofilm formation of S. aureus was to a great extent inhibited by these three AMPs.
Experience and conclusions:
- Peptides has shown their antimicrobial activity on S. aureus. And they can successfully expressed in E. coli.
- AgrA, which is an essential protein in quorum sensing construct, signifies toxicity to cells during growth.
- We have trouble in obtaining enough plasmid DNA extracted from L. lactis after transformation, probably due to either low copy number or low yield from the extraction protocol.
Collaborators and Supporters
